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Summary of Contents for Parker PICOSPRITZER III
- Page 1 Parker Hannifin Precision Fluidics Division 45 Route 46 East Pine Brook, NJ 07058 973-575-4844 http://www.parker.com/ppf ® PICOSPRITZER III Manual Pressure Systems for Ejection of Picoliter Volumes in Cell Research...
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Page 2: Safety
PICOSPRITZER III SAFETY Please read this entire set of instructions before attempting to use this manual for intracellular or extracellular ejections. This instrument should only be used as specified by the manufacturer. Use other than as specified may present a safety hazard. It is intended for research purposes only. Not for use on humans or for diagnostic purposes. -
Page 3: Table Of Contents
The system comes complete with high speed valve(s) and the necessary tubing assemblies (less pipette and holder). It is designed to fit a standard 19" relay rack. The Picospritzer III comes fully adjusted and ready to use. http://www.parker.com/ppf Page 3... -
Page 4: Operating Specifications
DO may be reduced by simply rotating the pressure NOT stretch the tubing out tight between the valve control knob counter-clockwise. housing and the pipette holder. http://www.parker.com/ppf Page 4... - Page 5 (or “snoop”) and watching for the formation of bubbles INDICATORS The Picospritzer III contains four LED indicators. at various connections. It is essential to have leak “ON” is the power indicator. The “PULSE” indicator...
- Page 6 Standard pipette holders are available for pipettes For additional flexibility in experimental use, the with diameters of 1.0, 1.2, 1.5 and 2.0 mm. Picospritzer III has a front panel BNC jack for each channel which permits independent activation of the two channels from external sources. The...
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Page 7: Background
(1, 3, 4). Brain Research 77:431 (1974) http://www.parker.com/ppf Page 7... - Page 8 Calibration chart provided courtesy of Dr. Joyce K. Ono, Department of Biological Science, California 15. Amaral, D.G. and Price, J.L. “An air pressure State University system for the injection of tracer substances into the brain.” Jrnl. Neuroscience Methods. 9:35-34 (1983) http://www.parker.com/ppf Page 8...
- Page 9 The X-intercept (15 MSEC) represents the mechanical lag time of the particular solenoid valve. The points of this graph are highly correlated (R=.97) with the independently determined specific activity (6.5 x 106 CPM/mL) of the radioactive solution. http://www.parker.com/ppf Page 9...
- Page 10 (70 MEGOHMS) containing 1 M ACh. The point to an input resistance test pulse. dose-responsive curve is shifted to the right because of desensitization from ACh leaking out of iontophoretic pipette. Each point is the mean response + standard error of the mean. http://www.parker.com/ppf Page 10...
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Page 11: Picospritzer® Iii Models
*XX should be replaced by your Pipette’s OD. Example: P/N 050-0010-GSK-KIT is for a 1.0 mm pipette holder. Each Kit comes with 10 replacement gaskets and 1 replacement nut. PICOSPRITZER® ACCESSORIES Pushbutton & Cable Assembly P/N 050-0000-800-1 Footswitch & Cable Assembly P/N 050-0000-801-1 http://www.parker.com/ppf Page 11... - Page 12 Note: Single channel and vacuum loading models have only one valve box and the “tee” fitting is omitted. PICOSPRTIZER® III MODELS A. Single Channel (Basic Model): The PICOSPRITZER III comes complete except for pipettes and pipette holders. One valve box and cable is supplied as well as the tubing required to connect it to the front panel mounted regulator assembly and to a pipette holder.
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