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Summary of Contents for Sartorius Stedim Biotech Sartoclear Dinamics Series
- Page 1 Manual Sartoclear Dynamics ® 85037-548-07 Vers. 02 | 2015...
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Page 2: Table Of Contents
Contents Introduction ................Liability . -
Page 3: Introduction
Liability The products described in this document are intended to be used for the clarification of mammalian cell cultures. Sartorius Stedim Biotech cannot assume liability if Sartoclear ® products are subjected to improper use. The use of this product for other applications or in another manner as described in this manual may result in personal injury or malfunction of the products. -
Page 4: Reference List
Reference List Sartoclear Dynamics ® DE Bags DE Content Quantity Order Number 0.5 Kg 29Z-B00005 1.5 Kg 29Z-B00015 5 Kg 29Z-B00050 10 Kg 29Z-B00100 Sartoclear Dynamics ® Order Number 29Z-K00001 Sartoclear Dynamics KIT contents: ® Filterline Powder Transfer Adapter Zipper Clamps for powder transfer Flexel 3D Magmix Bags... -
Page 5: Additional Equipment To De Bags
Additional Equipment for DE Bags Description Order Number Fork lift (D) 2ZG---0003 Adapter (D1) 2ZG---0004 Sartoclear Dynamics Filter ® Description Order Number Sartoclear Dynamics Filter 29Z-H00001 ® Description Order Number Sartoclear ® Manifold Set 29Z-S00001 Reference List... -
Page 6: Sartoclear ® Pilot Filter Holder
Sartoclear Pilot Filter Holder ® Description Order Number Sartoclear ® Pilot Filter Holder for 1 – 6 + 0.21 m ² 2ZGL--0005 Sartoclear Dynamics filters Drip Pan for Sartoclear Pilot Filter Holder 2ZGL--0008 ® Reference List... -
Page 7: Directions For Use
Directions for Use Material Sartoclear Dynamics ® is a group of products which together form a complete unit operation. A complete list for reference can be found above. Installation descriptions Filters (E) and the Sartoclear 1. Installation of the Sartoclear Dynamics ®... - Page 8 Place the required amount of Sartoclear Dynamics Filters between the ® INLET and the OUTLET plate. The text “THIS SIDE UP” and the Order and Lot# should be visible from above. The optimal amount of Sartoclear Dynamics filters is relative to the total wet cell ®...
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Page 9: Installation Of The Powder Transfer Adapter Kit
Clamp the Sartoclear Dynamics Filters together with the IN- and OUTLET plates ® with a clamping force of 25 kN. Connect the inlet and outlet tube to the inlet plates with 1½” tri-clamp (TC) After applying a clamping force of 25 kN the silicone seals inside the Sartoclear Dynamics ®... -
Page 10: Process Descriptions
For installation of the Magmix Bag please follow the instructions for installation of the Magmix Bag. If you need to calibrate the pH sensor, follow the directions for use of “pH probe calibration and insertion in a Flexel ® 3D Bag for LevMixer ®... -
Page 11: Addition Of The Filter-Aid
Addition of the filter-aid Connect the Filterline (B1) to the Palletank ® for Magmix Bags. Open the quick connector on the Magmix Bag and connect the Filterline. Take care that the vent filter does not get in contact with the liquid. Directions for Use... - Page 12 In order to minimize the shear stress on cells it is recommended to add the filter-aid just before the start of the filtration. After powder addition it takes only 5 – 10 minutes to suspend the DE. KIT (B) to connect the Sartoclear Dynamics Use the Sartoclear Dynamics ®...
- Page 13 Take the Zipper (B3) and insert the rails of the Sartoclear Dynamics DE Bag ® in the Zipper. Around 1 cm of the rails should come out. (See also 3d-print on the Zipper for a correct insertion of the rails). Insert the rails of the Powder Transfer Adapter in the other inlet of the Zipper so that the rails of the Sartoclear Dynamics ®...
- Page 14 Bend your fingers and pull so that the rail opens. The rail should open easily. The port of the connected Sartoclear Dynamics ® DE Bag and the Magmix Bag is now opened. Push the yellow folding plates inwards so that the plastic film completely covers the rails to prevent that DE will stick to the rails.
- Page 15 The Filterline (B1) must be opened before you release the powder. As soon as the Clamps for powder transfer are positioned correctly the plastic bar can be opened to release the powder. Gently shake the DE Bag up and down to speed up the transfer of DE Directions for Use...
- Page 16 In order to speed up powder uptake you may temporarily increase the stirring speed of the Magmix drive unit. See also table 1 for the recommended mixing speed per suspension volume for suspending the DE in the cell culture fluid. Table 1: Recommended mixing speed per volume Suspension volume (cells &...
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Page 17: Filtration
Disconnect the emptied DE Bag by pulling the Zipper backwards. The Powder Transfer Adapter and the emptied DE Bag will be closed and disconnected. Check if the rail of the powder transfer is completely closed before you connect a new DE Bag. A maximum of 5 DE Bags per Powder Transfer Adapter can be used. Start the filtration after the DE is completely suspended (5 –... -
Page 18: Recovery Optimization
For an easier handling of your emptied Magmix Bag after use you can close the vent filter at 50% volume capacity so that the Magmix Bag shrinks during processing. Switch the pump to constant pressure mode if the pressure reached 1.5 bar (21.75 psi) Stop the filtration when the flow rate goes down to 150 L/m In cases the filtrate has to be neutralized for the subsequent unit operation it... -
Page 19: Disassembling
Disassembling In order to prevent spilling of fluid on the floor an optional drip pan (2ZGL--0008) can be used. Release the pressure from the hydraulic clamping system (see the manual of your filter holder) For Sartoclear Pilot Filter Holder (on the picture) the pressure can be released ®... - Page 20 Lift and pull the filters in such a way that the rounded edge on the bottom edge of the filter is supported by the rail that is closest to you. Pull the filter out of the holder until the second groove reaches the first rail. Directions for Use...
- Page 21 Push the filter down so that the second grip can be reached for easy handling. Directions for Use...
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Page 22: Disposal
Return of used products In the case where a return of the materials to Sartorius Stedim Biotech is necessary, please ensure these materials are properly sterilized prior to shipment back to Sartorius Stedim Biotech. - Page 24 Sartorius Stedim Biotech GmbH. The status of the information, specifi- cations and illustrations in this manual is indicated by the date given below.
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