Slide Preparation; Tissue Preparation; Staining - Leica Aperio GT 450 User Manual

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When preparing slides for scanning, you must use the procedures required by the vendors of the stains and reagents

you are using.

Well prepared slides are crucial to a successful scan. After reading this section, if you have questions about whether

your slide preparation techniques will provide good scan quality, contact Leica Biosystems Technical Services.

Always consult the material safety data sheets for the stains and re-agents you are using, as well as your institution's

biosafety policies and procedures regarding safely handling biological materials.

It is the responsibility of the lab to verify the quality of the tissue preparation, the physical characteristics of the slide, and

the staining quality.

For optimal scanner performance, slides must be well-prepared.

Reproducibility of the slide staining is important for consistent and accurate diagnosis.

Make sure that the variations of the staining process are controlled and eliminated to the greatest extent possible.

Use appropriate morphological studies and controls as specified in the reagent manufacturer's instructions.

Avoid excessive stain residue, as this may affect scanner performance.

Slide Preparation

Many scanning issues are avoided by checking slide quality. Ensure the following:

Slides are very clean and in good condition. Wipe them with a clean cotton cloth (don't use chemical

cleaners). Make sure the slides have no dirt, no fingerprints, no markings, no writing, no extra mounting media, no

broken slides, no chips, no scratches, etc.

Slides are fully cured (not "wet").

All slides have coverslips. Coverslips are required when using the Aperio GT 450 scanner.

There is no mounting adhesive around the edges of a slide. This can cause it to stick or catch in the scanner

stage area.

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