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Bead Size and Optimal Operation
The size of paramagnetic beads affects their time to pellet, suspension
dynamics, and performance .
Adjust Protocols for Large Paramagnetic Beads
Larger paramagnetic beads exert greater magnetic force than smaller
beads, resulting in a faster time to pellet . This dynamic may cause larger
paramagnetic beads to pull off the bead capture strip prematurely . This
can be avoided by raising the height of the bead capture strip . Refer to
Adjust the Collection Height
on page 22 .
Figure 22: Magnetic Intensity and Paramagnetic Bead Size
Adjust Protocols for Small Paramagnetic Beads
Smaller paramagnetic beads exert less magnetic force than larger beads,
resulting in a slower time to pellet . This dynamic may result in longer
capture times . Gilson recommends waiting a few moments after the bead
discoloration in the wells has cleared before advancing to the next well
column .
TIME TO PELLET
Figure 23: Paramagnetic Bead Size and Time to Pellet
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EXTRACTMAN®
Operation
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