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Varian ProStar 335 Driver Manual

Photodiode array detector control
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Varian Analytical Instruments
2700 Mitchell Drive
Walnut Creek, CA 94598-1675/USA
ProStar 335 Photodiode
Array Detector Control
Galaxie Driver Manual
©Varian, Inc. 2002

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Summary of Contents for Varian ProStar 335

  • Page 1 Varian Analytical Instruments 2700 Mitchell Drive Walnut Creek, CA 94598-1675/USA ProStar 335 Photodiode Array Detector Control Galaxie Driver Manual ©Varian, Inc. 2002...

  • Page 2: Table Of Contents

    Configuring the Varian ProStar 335 Photodiode Array Detector........ 15 Configuring the Synopsis ................... 21 Building a Method.................. 24 Creating a New Method ....................24 Control Method Section for the Varian ProStar 335 Photodiode Array Detector....27 Signal Control ......................29 Purity Control......................31 Analog Control......................33 Peak Sensor Control ....................

  • Page 4: Introduction

    General This document describes the installation and configuration of the Varian ProStar 335 Photodiode Array Detector driver. It also explains how to build a control method for this driver, how to start and view a running acquisition and what actions are available during an analysis.

  • Page 5: Installation And Configuration

    Browser by running Install.exe from the root directory of the CD. 2. From the CD Browser click on the Install button and then click on the Drivers for Varian LCs or GCs button. 3. Then click on the Drivers for Varian LCs button. The following screen will appear:...
  • Page 6 Agreement and enter your driver Serial Number in the corresponding field. The serial number is provided on your driver serial number card and is different from the Galaxie serial number. Note that the serial number is case sensitive. ProStar 335 Photodiode Array Detector Control Manual - 5 -...
  • Page 7 5. If you want to reinstall or upgrade your drivers, you will need to run the setup (steps 1, 2 and 3) again. The following screen will appear: ProStar 335 Photodiode Array Detector Control Manual - 6 -...

  • Page 8: Configuring The Varian Prostar 335 Communications

    Use this button only when directed by a technical support representative. Configuring the Varian ProStar 335 Communications Ethernet Communications The Varian ProStar 335 Photodiode Array Detector uses Ethernet as the method of communications for instrument control. ProStar 335 Photodiode Array Detector Control Manual - 7 -...
  • Page 9 Ethernet network and needs to be given a unique IP address to identify the instrument. A static IP address can be also configured in the Varian Prostar 335 with the driver. A BOOTP Server is used to send IP addresses to TCP/IP devices.
  • Page 10 IP address and will list their Ethernet (MAC) addresses. The following screen appears: Power on the needed instruments; their Ethernet (MAC) addresses will appear a few seconds later. ProStar 335 Photodiode Array Detector Control Manual - 9 -...
  • Page 11 Network interface previously assigned to the instrument. NOTE: Do not check the Star 800 MIB box when assigning a Varian Prostar 335 Photodiode Array detector. To set up 800 MIB BOOTP, use the Star 800 MIB Configuration program from the START / PROGRAMS / GALAXIE menu.

  • Page 12: Configuring The Driver

    1. Select the Stations/Systems tab and then select the Systems item on the top tree view. Right click and a pop-up menu appears. 2. Select New to open the Create / Edit System wizard. ProStar 335 Photodiode Array Detector Control Manual - 11 -...
  • Page 13 Then, press Next. 5. In the last screen of this wizard (shown below), install the device to be configured in the desired instrument by clicking ProStar 335 Photodiode Array Detector Control Manual - 12 -...
  • Page 14 Select the Varian ProStar 335 Photodiode Array Detector driver. 6. Click on the OK button. The Create / Edit System screen shown above appears again with the name of the newly installed device. ProStar 335 Photodiode Array Detector Control Manual - 13 -...
  • Page 15 7. When all the required devices are installed, click on the OK button to accept the list. A new screen appears and asks if the instrument devices should be configured. If the answer is Yes, the instrument device configuration screens appear. ProStar 335 Photodiode Array Detector Control Manual - 14 -...

  • Page 16: Configuring The Varian Prostar 335 Photodiode Array Detector

    The toolbar allows you to easily scroll through the configuration windows: to select the first configuration window. to select the previous configuration window. to select the next configuration window. to select the final configuration window. ProStar 335 Photodiode Array Detector Control Manual - 15 -...
  • Page 17 In the IP Address field, enter the IP address of the instrument. Press the button to search the subnet for all connected Varian Prostar 335 instrument. The following window will be displayed. ProStar 335 Photodiode Array Detector Control Manual - 16 -...
  • Page 18 Check the Enable Ready In box to enable this option. When this option is enable the Varian Prostar 335 waits a ready in signal to go to the ready state. NOTE: For more details about the BOOTP configuration, refer to the Ethernet Communications section.
  • Page 19 The detector will always operate in extended range mode with a prep flow cell. ProStar 335 Photodiode Array Detector Control Manual - 18 -...
  • Page 20 Ethernet communication using BOOTP. To set the Ethernet Parameters fill the following fields. IP Address: Enter the static IP Address Subnet Mask: Enter the subnet mask Default Gateway: Enter the default gateway ProStar 335 Photodiode Array Detector Control Manual - 19 -...
  • Page 21 Click on the button to send the new Ethernet Parameters to the instrument. Then reboot the instrument to apply the new Ethernet Parameters. ProStar 335 Photodiode Array Detector Control Manual - 20 -...

  • Page 22: Configuring The Synopsis

    Click on to define a background color, or on make the background transparent. Click on the icon to change the grid color. ProStar 335 Photodiode Array Detector Control Manual - 21 -...
  • Page 23 (wavelength, signal, etc.) depending on the installed devices. For example, the following pictures represent the synopsis of the Varian ProStar 335: Galaxie Configuration Manager screen Galaxie Chromatography Data System screen ProStar 335 Photodiode Array Detector Control Manual - 22 -...
  • Page 24 Control and View boxes in the Galaxie Configuration Manager Projects Properties. For more details about the creation and configuration of a system, refer to the Galaxie Configuration Manager User’s Guide. Click on the button to print the current configuration. ProStar 335 Photodiode Array Detector Control Manual - 23 -...

  • Page 25: Building A Method

    1. To create a new method, select the FILE / NEW METHOD option from the Galaxie Chromatography Data System main menu. A wizard guide will appear and provide assistance during the first steps of method creation: ProStar 335 Photodiode Array Detector Control Manual - 24 -...
  • Page 26 Varian ProStar 335 Photodiode Array Detector is detailed. Other parameters such as Injection Volume, Vial and Rack number may be necessary and are defined in the acquisition section. ProStar 335 Photodiode Array Detector Control Manual - 25 -...
  • Page 27 NOTE: For more details about the programming of each method section, turn to the Galaxie Chromatography Data System User’s Guide. ProStar 335 Photodiode Array Detector Control Manual - 26 -...

  • Page 28: Control Method Section For The Varian Prostar 335 Photodiode Array Detector

    The following screen appears: Press the button to display the System Control Method Advanced Tools window: to send the current method to the instrument. ProStar 335 Photodiode Array Detector Control Manual - 27 -...
  • Page 29 ProStar 335 Photodiode Array Detector Control Manual - 28 -...

  • Page 30: Signal Control

    Wavelength field. The entered value must be greater than 190 Enter the ending wavelength for spectral collection in the Max wavelength field. The entered value must be less than 950 nm. ProStar 335 Photodiode Array Detector Control Manual - 29 -...
  • Page 31 Galaxie run. Check the Lamp Off at the end sequence to enable this option. When this option is enable the Varian Prostar 335 lamps will be turned off at the end of a Galaxie sequence.

  • Page 32: Purity Control

    For a given wavelength range, the Purity value is the average wavelength weighted by the square of the absorbance. ∑ λ ² Purity ∑ ² With $L DEVRUEDQFH DW ZDYHOHQJWK i ProStar 335 Photodiode Array Detector Control Manual - 31 -...
  • Page 33 Enter the viewing wavelength for the purity calculation in the Viewing wavelength field. The entered value must be between the min and max purity wavelength. Enter a threshold value for the purity calculation in the threshold field. ProStar 335 Photodiode Array Detector Control Manual - 32 -...

  • Page 34: Analog Control

    Peak Ticks are small spikes added to the analog output to indicate when the Peak relay is activated. Enter the time program for the Wavelength, the Attenuation, and the Autozero parameters. ProStar 335 Photodiode Array Detector Control Manual - 33 -...
  • Page 35 Use the following buttons to edit the table: to add a line to the table. to delete a line in the table. to clear the entire table. ProStar 335 Photodiode Array Detector Control Manual - 34 -...

  • Page 36: Peak Sensor Control

    The time entered for the Duration will be dictated by the specifications of the particular sample collector or column switching valve used. Generally a duration of 0.5 seconds will satisfy most applications. ProStar 335 Photodiode Array Detector Control Manual - 35 -...
  • Page 37 When this function is activated the signal going above or below a threshold activate the Level Sense relay. It is necessary to adjust ProStar 335 Photodiode Array Detector Control Manual - 36 -...
  • Page 38 On Time 2.5 min, Off Time 12.7 min. This parameter cannot be set at initial conditions (time 0.00). Time slicing may also be triggered by ProStar 335 peak or level sensing. On Peak triggering turns on time slicing when a peak is detected, Off Peak triggering turns off time slicing when any peak event is detected.

  • Page 39: Relay Control

    Each step of the program includes time and state for each relay’s new values. Use the following buttons to edit the table: to add a line to the table. ProStar 335 Photodiode Array Detector Control Manual - 38 -...
  • Page 40 ProStar 335 Photodiode Array Detector Control Manual - 39 -...

  • Page 41: Starting And Viewing An Acquisition

    Quick Start (Single Acquisition). 1. Press the button. In the Quick Start window, select the system, the method and optionally the project if connected in "All project" mode. 2. Press the OK button. ProStar 335 Photodiode Array Detector Control Manual - 40 -...
  • Page 42 In a multiple injector system, the same parameters entered for the first injector must be entered for the other injectors before starting the acquisition. ProStar 335 Photodiode Array Detector Control Manual - 41 -...

  • Page 43: Sequence

    3. Start the sequence by clicking on the button. NOTE: For more details about how to start an acquisition, refer to the Galaxie Chromatography Data System User’s Guide. ProStar 335 Photodiode Array Detector Control Manual - 42 -...

  • Page 44: Running Acquisition Status

    A toolbar allows you to easily scroll through the status windows: to select the first status window. to move back to the previous status window. to move forward to the next status window. to select the final status window. ProStar 335 Photodiode Array Detector Control Manual - 43 -...
  • Page 45 The light colors change depending on the detector state (Lamp On/Off, Method Ready/Not Ready/Monitoring, etc.). Click on to start or stop the D2 lamp of the detector. Click on to start or stop the visible lamp of the detector. ProStar 335 Photodiode Array Detector Control Manual - 44 -...
  • Page 46 Check on uncheck the Relay x On check box to close or open the corresponding relay. Click on the button to send the analog outputs and relays parameters to the instrument. ProStar 335 Photodiode Array Detector Control Manual - 45 -...
  • Page 47 Error log or the Run log from the instrument. Select with the dropdown list which log you want to upload. About Window Click on the button to calibrate the instrument with the lamp when required. ProStar 335 Photodiode Array Detector Control Manual - 46 -...
  • Page 48 The lights indicate the state of the detector. Red light indicates if a fault has occurred. The current wavelength is displayed. Click on the button the start the autozero of the detector. ProStar 335 Photodiode Array Detector Control Manual - 47 -...
  • Page 49 ƒ Not ready: contains the not ready information sent by the instrument. ƒ Events: contains the events and errors that have occurred on the instrument, listed below: ProStar 335 Photodiode Array Detector Control Manual - 48 -...

  • Page 50: Appendix

    Peak Sensor Functions This section describes the four different functions that activate the Peak Sensor relays of the ProStar 335 Detector. These functions are named Peak Sense, Level Sense, Timeslice, and Pulse Relay. The Peak Sensor relay is only activated in the following states: ready, running and stopped.
  • Page 51 Select the peak of interest and then Width 50% and the corresponding value is displayed (in minute). Based on your assessment of what may constitute noise, or unwanted peaks adjust the S/N value either up or down from the preset 8. ProStar 335 Photodiode Array Detector Control Manual - 50 -...
  • Page 52 The Peak Sense function will continue to detect peak start, peak end, and peak valley, until it is turned off by time programming or the end time of the run is reached. Peak Sense Activation by Time ProStar 335 Photodiode Array Detector Control Manual - 51 -...

  • Page 53: Level Sense Function

    The Peak Sense or Level Sense functions do not have to be on in order to use the Time Slice function that is triggered by Peak Sense or Level Sense. ProStar 335 Photodiode Array Detector Control Manual - 52 -...

  • Page 54: Pulse Relay Function

    It is used to send a single relay actuation to the sample collector at a specified time for any purpose you may determine (suppose you estimate that your sample receiver will fill ProStar 335 Photodiode Array Detector Control Manual - 53 -...
  • Page 55 3 minutes. You may set the Pulse function to advance the sample collector at 2.75 minutes to the next sample receiver). ProStar 335 Photodiode Array Detector Control Manual - 54 -...

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