Page 1 ProStar 325 UV-Vis Variable Wavelength Detector Service Manual Installation Category II Pollution Degree 2 Safety Class 1 (EN 61010-1) Publication Number 85 102009 00...
Page 2 ProStar325 Service Manual This page is intentionally left blank. Publication date 8 May 2003...
Page 3 ProStar325 Service Manual Varian has offices in most countries. The major offices for optical spectroscopy products are listed below: Varian Australia Pty Ltd (Manufacturing site) 679 Springvale Road, Mulgrave, Victoria 3170 Australia International telephone: + 61 3 9560 7133 International fax: + 61 3 9560 7950 Varian Inc.
Page 4: Table Of Contents
General Information ..........9 1.1 How to use this manual 1.1.1 Finding Information 1.2 General Information 1.3 Design features 1.4 Fundamentals of the ProStar 325 detector 1.5 Detector performance testing 1.6 Safety practices and hazards 1.6.1 Electrical hazards 1.6.2 Lamp source 1.6.3...
Page 5 Inspection for transit damage 2.10 Computer interface 2.10.1 Computer requirements 2.10.2 Ethernet 2.10.3 Recommended printer/plotter 2.11 Setting up your PC 2.11.1 ProStar 325 Verify software 2.12 Operator training Installation ............30 3.1 Introduction 3.2 Pre-installation 3.3 Installation times 3.4 Before you start 3.5 Installation...
Page 6 ProStar325 Service Manual 3.18 Front Panel LEDs 3.19 Detector States 3.20 Instrument performance tests 3.21 Flowcell 3.21.1 Tubing 3.21.2 Flowcell storage 3.21.3 Cleaning the flowcell 3.21.4 Flowcell types 3.22 Back pressure restrictor 3.23 Extended range 3.24 Source lamps 3.25 Cleaning 3.26 Excessive Noise and/or Drift Electronics ............64 4.1 Introduction...
Page 7 ProStar325 Service Manual 4.9.2 Dual Photodiode PWB 4.9.3 LED – IrDA PWB 4.9.4 Power Supplies 4.9.5 Fan replacement 4.9.6 Fuses Optics..............85 5.1 Introduction 5.2 Optics module 5.2.1 UV lamp assembly 5.2.2 Visible lamp assembly 5.2.3 Monochromator 5.2.4 Optical path 5.3 Removal and replacement procedures 5.3.1 UV lamp source 5.3.2...
Page 8 ProStar325 Service Manual 7.6.3 Menu task bars 7.6.4 Status bar 7.7 Main Display 7.8 Method Development 7.8.1 Building a method 7.8.2 Modifying a method 7.8.3 Downloading a method 7.8.4 Saving a method 7.9 Detector Status 7.9.1 Main 7.9.2 Internal Method 7.9.3 Logs 7.9.4...
Page 9 ProStar325 Service Manual Publication date 8 May 2003...
Page 10: General Information
All users of the ProStar 325 detector must be familiar with the correct operating procedures. Site preparation and guidelines are described in the pre-installation section of this manual and in the Hardware Operation Manual publication number 85-102016-00.
Page 11: Design Features
README file, Service Alerts or Service notes. General Information This section provides an overview of the ProStar 325 detector and contains important information on; • Design features • Fundamentals of the ProStar 325 •...
Page 12: Fundamentals Of The Prostar 325 Detector
The detector’s advanced optical design, electronics, photo diode detectors and range of precision dual-path flowcells, make the ProStar 325 a versatile detector capable of operating in different modes. The detector can come in four different configurations; single-wavelength with deuterium lamp...
Page 13 ProStar325 Service Manual variations of a common base model that can be configured for each individual application. If fitted with the optional Vis lamp, the detector operates across the entire spectrum from 190nm to 900nm. Both lamps are continuum lamps and only one should be selected at a time. Wavelength selection is provided by a holographic grating driven by a stepper motor drive mechanism.
Page 14: Detector Performance Testing
Observe all relevant safety practices at all times. 1.6.1 Electrical hazards The ProStar 325 detectors contain electrical circuits, devices, and components operating at dangerous voltages. Contact with these circuits, devices and components can cause death, serious injury or painful electrical shock.
Page 15: Lamp Source
Application of the wrong supply voltage can create a fire hazard and a potentially serious shock hazard and could seriously damage the ProStar 325 system and any attached ancillary equipment. Replace blown fuses with fuses of the size and rating as stipulated in the text adjacent to the fuse holder at the rear of the detector or, as specified in the manual.
Page 16: Other Precautions
Always switch off the detector before changing a lamp. 1.6.4 Other precautions Do not block the rear ventilation grills on the ProStar 325. Make sure you provide adequate space behind the detector for air circulation, electrical, and communications cables. Warning: The use of the ProStar 325 system may involve materials, solvents and solutions that are flammable, corrosive, toxic or otherwise hazardous.
Page 17: Service Policy
ProStar325 Service Manual 1.7.2 Service policy In the normal course of field servicing, it is Varian’s policy to troubleshoot and repair to a modular level at the detector’s location. Software diagnostic routines are available to aid in fault isolation. These are to be used in conjunction with conventional test equipment to effect on-site repairs.
Page 18: Oem Warranty
1.7.5 OEM Warranty Personal Computers (PC’s), printers, modems other PC accessories and OEM accessories used with the ProStar 325 are not covered by the Varian detector warranty. Generally this type of equipment is sourced locally and therefore local supplier or vendor warrantees will apply.
Page 19 ProStar325 Service Manual Publication date 8 May 2003...
Page 20: Pre-Installation
Pre-installation Checklist contained in this section. Strike out any entries not appropriate to your system, and then send a copy of the checklist to the Varian Sales and Service Office or local Varian agent dealing with the supply of the equipment.
Page 21 ProStar325 Service Manual A ‘Note’ is used to give advice or information. Read all warnings and cautions carefully and observe them at all times. A triangular symbol indicates a warning. The meanings of the symbols that may appear alongside warnings in the documentation or on the detector are;...
Page 22 ProStar325 Service Manual Information symbols The following symbols appear on the ProStar 325 detector to provide you with additional information; Mains power on Mains power off Fuse Single phase alternating current Focus Vertical adjustment Horizontal adjustment When attached to the rear of the product, indicates...
Page 23 Consult the dealer or an experienced radio/TV technician for help CE-compliant products ProStar 325 detectors have been designed to comply with the requirements of the Electro-magnetic Compatibility (EMC) Directive and the Low Voltage (safety) Directive (commonly referred to as the LVD) of the European Union.
Page 24: Pre-Installation Checklist
All applicable preparations have been completed. Please arrange for the installation to be completed. I fully understand that charges may be levied if the installation by Varian Service personnel is delayed due to facilities not being prepared in accordance with the information contained in this manual.
Page 25: Pre-Installation Complete
All preparations have been completed. To arrange installation of the detector, complete the details on the back of this sheet and return a copy of the form to the Varian Office shown below. Note: The remainder of this manual contains the environmental requirements and technical specifications for the ProStar 325 detector, and should not be discarded.
Page 26 20–25 °C and is held constant to within ±1 °C through the working day. Caution The ProStar 325 detector is designed for operation in clean air conditions. The laboratory must be free of all contaminants that could have a degrading effect on the detector’s components.
Page 27: Workbench
ProStar325 Service Manual Humidity Temp (%RH) (°C) Condition Altitude non – condensing Non-operating 0–2133m 5–45 20–80 (transport) (0–7000') Non-operating & meeting sea level 90–95 dielectric strength tests Operating but ≤80 necessarily 0–2000m 5–31 ≤[80–3.33(t– meeting (0–6562') 31–40 31)] performance specifications 0–853m Operating (0–2800')
Page 28: Electrical Specifications
Do not plan to use extension cords or outlet adaptors. All ProStar 325 detectors are supplied with a 2metre (6’ 6") long power cord and three-pin plug assembly that is compatible with common standards applicable in the local area.
Page 29: Fuses
Insurance As the carrier’s liability ceases when the equipment is delivered, Varian recommends that the detector owner arrange a separate insurance to cover transportation from the delivery point to the installation site. The delivery point will vary according to the carrier, the shipping method and in some cases the terms of sale.
Page 30: Inspection For Transit Damage
Varian Sales Office. All contents of the shipping packages should be assembled together when installation is to be carried out by Varian Service personnel. Do not discard any packaging components or filler materials. 2.10 Computer interface 2.10.1...
Page 31 6 or Windows XP® Windows XP® Varian can supply a PC for the ProStar 325 in the recommended configuration. Consult your local salesperson for details. The operating system software will be pre-loaded. All software disks and manuals are to be supplied to the customer.
Page 32: Ethernet
ProStar325 Service Manual 2.10.2 Ethernet The ProStar 325 detector requires an Ethernet connection to the PC via a shielded twisted pair Ethernet crossover cable Category 5 or better (provided with the detector). If connection is required to an Ethernet network, then a shielded twisted pair Ethernet non-crossover cable Category 5 or better will be required (not supplied).
Page 33: Operator Training
Service Office. As a guide to the time required for installation by Varian service, a typical ProStar 325 system will be completed and ready for use within four hours. The installation time can be kept to a minimum by ensuring proper preparation of the site and arrangement of all units to allow for easy access.
Page 34 ProStar325 Service Manual Publication date 8 May 2003...
Page 35: Installation
• ProStar325 Hardware operation manual (85 102015 00) • Declaration of conformity • Factory test certificates Instructions for installing the ProStar 325 detector and verifying its performance using the Verify software, together with an overview of the system, are included in this section. Detailed operating procedures are provided in the Hardware Operation Manual and also included in the on-line help.
Page 36: Pre-Installation
ProStar325 Service Manual Pre-installation Before receiving the ProStar detector the customer will have been provided with a Hardware manual (part number 85 102015 00); which describes the environmental and operating requirements of the ProStar system and details the facilities which must be provided to ensure that the system can be effective.
Page 37: Installation Times
Only the lamps and flowcell assembly can be removed by the operator. All other panels and covers may be removed only by Varian-trained, qualified or authorized Customer Support Representatives. Publication date 8 May 2003...
Page 38: Installation
ProStar325 Service Manual Installation A quick reference guide for CSR’s to install the ProStar325 detector is given below to ensure that you complete each step of the installation procedure. You may need to refer to this information if you re-locate the instrument. At the end of this section there is an Installation Acceptance Checklist which you must complete during installation of the detector.
Page 39: Installation Of Door Cap
If the ProStar 325 is the top module in the stack, the door cap should be installed prior to installing the door on the module. If the ProStar 325 is not at the top of the stack, do not install the cap and proceed to the next section describing door installation.
Page 40 ProStar325 Service Manual Magnetic door latch Adhesive side Figure 1 Door and Door Cap Orientation Publication date 8 May 2003...
Page 41: Installation And Removal Of Door
Figure 2 Door and Door Cap Assembly Installation and Removal of Door The module door may be attached to the front of the ProStar 325 to cover the tubing connections to the flowcell, see below. Latch Plate...
Page 42: Software Installation
ProStar325 Service Manual Orientate the door so that the magnetic door latch lines up with the latch plate. Insert the top hinge pin into the top hinge. Gently press down on the top of the door (as shown in Figure 3) and slide the lower hinge pin into the lower hinge and stop pressing.
Page 43: Ethernet Connection
Detectors and Workstations. The term Ethernet refers to the cables and interface cards that are used to connect devices on the network. Several types of Ethernet cables exist, and if you are connecting your ProStar 325 Publication date 8 May 2003...
Page 44: Where To Begin
TCP/IP. Since the ProStar 325 Detector uses TCP/IP to communicate with the Star Workstation, the Workstation PC and the ProStar 325 Detector need to be given unique IP addresses — the addresses that are used to identify each networked TCP/IP device.
Page 45: Installing And Configuring The Ethernet Card In Your Pc
Ethernet Board from the computer to remove the incorrect Ethernet drivers. You must configure your computer’s network settings to allow communication with the ProStar 325 Detector. You do so by running the Windows Control Panel. 1. Run the Windows Control Panel by selecting it from the Start menu (under Settings).
Page 46 ProStar325 Service Manual Double-click on the Network Connection icon to set your Network options. 3. Double click the LAN icon and the following LAN Connection Properties dialog box is displayed. If TCP/IP is already listed in the list of network components, skip to the Note after step 5…...
Page 47 ProStar325 Service Manual 4. The Select Network Component Type dialog box is displayed. Select Protocol and click on the Add button. 5. The Select Network Protocol dialog box is displayed. Select TCP/IP as the protocol. Select Microsoft as the manufacturer. Click OK.
Page 48: Configuring Tcp/Ip Parameters With No Company Network
ProStar325 Service Manual 3.9.4 Configuring TCP/IP Parameters with No Company Network The following three steps assume you have completed steps 1 through 5 from the previous procedure. 1. Select TCP/IP from the network component list and click on the Properties button. The TCP/IP Properties dialog box is displayed.
Page 49 ProStar325 Service Manual Ensure that no entries have been made in your Gateway, and that Domain Name Server (DNS) and WINS Configuration have been disabled. These entries are not used in an isolated network and may cause problems if they are present.
Page 50: Configuring Tcp/Ip Parameters For A Company Network
ProStar325 Service Manual 3.9.5 Configuring TCP/IP Parameters for a Company Network The following three steps assume you have completed steps 1 through 5 from the procedure Installing and Configuring the Ethernet Card in Your PC. 1. Select TCP/IP from the network component list and click on the Properties button.
Page 51 ProStar325 Service Manual Contact your Network Administrator to see what the appropriate settings are for your Gateway, and whether Domain Name Server (DNS) and WINS Configuration are needed. 3. Your Network Administrator may also instruct you to specify parameters in the Bindings and Advanced tabs. 4.
Page 52: Hand Held Pc Connection
ProStar325 Service Manual 3.10 Hand Held PC connection The ProStar325 detector has the capability of being controlled by a hand held pc via its inbuilt infra-red link. The detector’s receiver window is located next to the three LED’s on the front panel.
Page 53: Instrument Setup
ProStar325 Service Manual 3.11 Instrument setup Cabling The monitor, printer/plotter and detector are all connected to the PC by cables that plug into the back of the PC. Consult your monitor and printer/plotter manuals for details of their cabling requirements. J1 Comm: RJ-45 type connector used to interface the ProStar325 to the PC by using a 100ohm shielded twisted pair Ethernet crossover cable Category 5 or better.
Page 54: Synchronization Signals At P9 And J10
ProStar325 Service Manual 3.12 Synchronization Signals at P9 and J10 These signals are used to synchronize the operation of a group of instruments that are not interfaced to a Workstation via a GPIB interface. P9 and J10 pin designations are shown below. The SYNC signals come in four pairs and define how the detector will operate in an HPLC system.
Page 55 1. The ProStar 325 discovers an internal fault condition that warrants aborting the run. 2. The ProStar 325 receives a Fault In signal and it has no internal fault condition itself. Autozero: This edge-triggered contact closure causes an autozero adjustment.
Page 56: Rear Panel Accessory Interface Pwb
The ProStar 325 UV/Vis Detector can be operated with any line voltage in the range of 100-240Vac +/-10%, 50-60 Hz. +/-1Hz.
Page 57: Fuses
ProStar325 Service Manual It is important for operator safety and proper operation of the ProStar 325 UV/Vis Detector that you use the power cord supplied, together with a properly grounded 3-wire power strip. 3.16 Fuses The ProStar325 contains two fuses which are located at the back of the instrument.
Page 58: Detector States
ProStar325 Service Manual LEDs Status Color Power Initializing Yellow flashing Power On Green Lamp Lamp On Green Fault Ready/Run Not Ready Ready/Stopped Green Method Running Yellow 3.19 Detector States The detector has 6 states NOT READY, INITIALISING, MONITOR, READY, RUNNING and STOPPED. Transition between the states will depend on the current state and a number of signals, specifically SYNC signals, and/or a number of internal events which can also cause state transitions.
Page 59 ProStar325 Service Manual NOT READY LAMP ON – Detector is not ready for starting a run. It will be in this state after a lamp on and EnableIn is high, and SyncInReady is also high. It will not leave this state unless it receives one of the following;...
Page 60: Instrument Performance Tests
ProStar325 Service Manual Detector SYNC signal outputs SYNC signal inputs State Enabl Read Start Fault Enable Ready Ready Start Fault e Out y Out (Now (Enabl (Enabl Softwa e In e In active switch) active active ignore ignore ignore ignore ignore READY active...
Page 61 ProStar325 Service Manual Wavelength accuracy Wavelength precision (repeatability) If any of the tests fail to meet the specifications you can refer to the Troubleshooting and Diagnostics Chapter of this manual to help diagnose and correct the problem. To start the installation tests using the Verify software: •...
Page 62 ProStar325 Service Manual • Click OK to continue. • Click START to start tests. • Enter in the customer details and instrument serial number and type. Each test will be run in succession, and the results for each test are displayed in the Report area immediately after the test is completed.
Page 63: Flowcell
ProStar325 Service Manual 3.21 Flowcell The ProStar325 can be fitted with any one of four flowcells (ordered separately). The customer’s chosen flowcell type is packed internally in the detector. The four types are; Part Number Description Volum 02 101818 00 FLOWCELL 9x0 15ul ANALYTICAL...
Page 64: Tubing
ProStar325 Service Manual • The replacement procedure is the opposite of this. Use care to align the flowcell on locating pins. 3.21.1 Tubing Each flowcell comes with a set of nuts & ferrules that are fitted to peek tubing and connected to the flowcell as shown. Part number Description Used on all...
Page 65 ProStar325 Service Manual Fit the nuts and ferrules on the peek tube as shown below. Screw these two tubes into the body of the flowcell. Do not over tighten as this will damage the threads. Each flowcell has an inlet and outlet connection. Publication date 8 May 2003...
Page 66: Flowcell Storage
ProStar325 Service Manual 3.21.2 Flowcell storage Prior to storage flush out the flowcell with Methanol or IPA. Leave the solvent inside the flowcell during storage by connecting a short length of tube between the inlet and outlet connections of the flowcell. To avoid possible degradation in performance due to dust, store flowcells in sealed plastic bags whenever the flowcell is not installed in the detector.
Page 67: Flowcell Types
ProStar325 Service Manual diluting one part of concentrated phosphoric acid with three parts of HPLC grade water. 3. After pumping 50ml of the 25% acid through the cell at 1-2 ml/min, rinse by pumping 100 to 200ml of HPLC grade water through the system.
Page 68: Back Pressure Restrictor
ProStar325 Service Manual 4x0.15 Super 4x0 Micro Prep 3.22 Back pressure restrictor The outlet port of the analytical and semi micro flowcells should be connected to a back pressure restrictor (provided in the ship kit). Also, the prep 9x1 prep flowcell should also be connected to a back pressure restrictor when it is used for analytical measurements.
Page 69: Extended Range
ProStar325 Service Manual 3.23 Extended range The ProStar325 detector can extend the working Absorbance range by calibrating the path length ratio between the long and short path cells. Extended range calculations are standard with all detectors. For cell configurations that do not support dual path operations, an error will be reported based upon the cell ratio calculated to be above 100.
Page 70: Cleaning
ProStar325 Service Manual and allow the lamps to cool. Caution Care must also be taken when removing lamps. Touching the glass envelope of either the UV or Visible lamp will reduce its efficiency. Never touch the glass surfaces of new lamps. Always handle a lamp by its base.
Page 71 ProStar325 Service Manual Publication date 8 May 2003...
Page 72 ProStar325 Service Manual Publication date 8 May 2003...
Page 73: Introduction
ProStar325 Service Manual Electronics Introduction The electronics system in the ProStar 325 detector provides the interface between the optical measurement system, including any accessories. Communications is either through an external host computer connected via Ethernet or Pocket PC communicating through a standard IrDa interface running as a Star workstation.
Page 74: Deuterium (D ) Lamp Power Supply
ProStar325 Service Manual The Processor PWB assembly contains a number of independent power supply circuits for regulating the voltage supplies needed by the instrument. Most of these supplies use linear regulators. Consequently the Processor PWB only requires low voltage input supplies for producing all the necessary voltages.
Page 75 ProStar325 Service Manual mode Q8 is regulating the current and the voltage feedback is from the D2 lamp’s cathode via D15. Warning If the polycarbonate safety cover is removed, the D2 supply voltage is exposed. Contact with this voltage may cause electric shock or death.
Page 76: Visible Lamp Power Supply
ProStar325 Service Manual Return an error 4.2.3 Visible lamp power supply The visible lamp supply is a continuous current buck converter operating at 200kHz. One side of the lamp is connected to +24V and the other side is regulated to be 12V down from that supply. U56 is a current mode controller.
Page 77: Processor Pwb Functions
ProStar325 Service Manual The use of an eight-layer PWB ensures that radiation from the printed tracks is reduced, because the tracks act as microstrip transmission lines relative to the contiguous groundplane. Small ferrites have been fitted to many signal lines that leave the Processor PWB.
Page 78 ProStar325 Service Manual JTAG output port IrDA interface Diagnostic functions Serial ports, I/O RS232 The memory is addressed by the 18 address lines from the Central Processing Unit (CPU). Additional “chip select” lines are internally decoded in the microcontroller to address individual blocks of memory.
Page 79: Non Volatile Data Storage
ProStar325 Service Manual When the instrument is powered up, the outputs of the Xilinx are all open circuit and have weak pullup resistors. The Xilinx need to be configured by downloading them with code called “Gateware”. When the microcontroller starts its boot sequence, it downloads the gateware along serial port lines.
Page 80: Signal Processing And A To D Conversion
ProStar325 Service Manual • Equipment name / Detector alias The contents of the CMOS can be read and changed through user software interface. In order that the CMOS memory is not lost when the battery is replaced, then replace the battery with the mains power on. 4.3.2.2 EEPROM The EEPROM contains the following information;...
Page 81: Peak Sensing
ProStar325 Service Manual to the inactive state (as defined by the value of the “active sense” parameter stored in the detector). Upon being triggered, relay activation will occur at the time interval equal to the “peak sense duration” parameter as stored in the method. The peak sense relay can be activated from any of the following sources (only one source can be active at any one time).
Page 82 ProStar325 Service Manual • Peak Valley – negative (down) • Peak End – positive (up) • Pulse – negative (down) • Time Slice – positive (up) • Level - positive (up) A tick mark will appear at the analog output in real time immediately after the event is detected, whereas peak sense relay activation will occur only after the time interval of the “peak sense duration”...
Page 83: Calibration
ProStar325 Service Manual 4.3.7 Calibration The electronics on the Processor PWB do not require any manual calibration. The instrument does however perform self- calibration at power-on. 4.3.7.1 Gain Calibration A Gain calibration is performed by the detector at power on. It is applied across the entire wavelength range at 2nm intervals.
Page 84: Initialization Sequence
ProStar325 Service Manual read at that wavelength). The results from wavelength#2 optional collections are available in the status message as well as the method run data stream. Autozeroing can be requested at both wavelengths, however, peak sense is not valid in Dual Wavelength mode. Bunching of data is also not available in dual wavelength collection.
Page 85: Diagnostic Select Switch
ProStar325 Service Manual Diagnostic select switch A 16-position thumbwheel switch is used to select service diagnostic routines. DESCRIPTION NORMAL INSTRUMENT OPERATION FLASH MEMORY CRC/ CHECKSUM BOOT BLOCK Continuously checks the boot block program. Display ‘10’ if OK Display ‘11’ if ERROR RAM INTEGRITY test Continuously loops through all SDRAM Display ‘20’...
Page 86: Dual Photodiode Pre-Amp Pwb
ProStar325 Service Manual DESCRIPTION DEUTERIUM LAMP test Enables the filament and then switches on the lamp. Displays ‘76’ during filament on Display “70” when lamp is operating VIS LAMP test (if fitted. This test will do nothing if there is no Vis lamp option present) Displays ‘86’...
Page 87: Led - Irda Display Pwb
ProStar325 Service Manual LED – IrDA display PWB The LED/IrDa display PWB contains a small IrDa Transceiver incorporating a photodiode when receiving data and an LED when transmitting data. There are three bi-color LEDs that indicate detector status. These are controlled by signals from the FPGA.
Page 88 ProStar325 Service Manual • Locate and remove the two screws at the rear of the detector used to secure the fan baffle. Then, swing the baffle to the right as shown. screws Publication date 8 May 2003...
Page 89 ProStar325 Service Manual • Locate and remove the two screws on the rear panel • Remove the optics module as described in the Optics section of this manual. • Remove the earth lug screw located on the Processor PWB. • Remove the single screw holding down the power supply module.
Page 90 ProStar325 Service Manual mains supply input cables are still connected. Place the power supply module in the service position as shown below, and fasten in this position using the screw which originally held it in place. The Processor PWB will now be exposed. •...
Page 91: Dual Photodiode Pwb
ProStar325 Service Manual The Processor PWB replacement procedure is the opposite of the removal procedure. Note: Whenever this PWB is replaced, you are required to perform a wavelength & 0%T calibration. Refer to the Diagnostics section for details. 4.9.2 Dual Photodiode PWB •...
Page 92: Led - Irda Pwb
ProStar325 Service Manual 4.9.3 LED – IrDA PWB • Remove the detector’s main cover. • The LED – IrDA PWB is located behind the front panel in the top left hand corner as you face the detector. • Disconnect the ribbon cable to this PWB. •...
Page 93: Fan Replacement
ProStar325 Service Manual • Disengage the safety cover and slide the power supply module toward the front of the detector (as described above in the Processor PWB removal procedure). • Remove the plastic protective cover and cut all cable ties around the internal cables in the supply module as shown.
Page 94 ProStar325 Service Manual Note: Always check the information printed on the rear of the detector for the most up-to-date details regarding the fuse type. To check a fuse - 1/. Disconnect the detector from the mains power supply. 2/. On the mains input connector (rear panel), lift the fuse cap and pull out the fuse holder (lower red section).
Page 95 ProStar325 Service Manual Slide the fuse compartment out. The two fuses are located on either side of the compartment. Publication date 8 May 2003...
Page 96: Optics
• Collect the light from the monochromator and direct it to the photodiode detectors. The ProStar 325 optics is designed around a Cerzy-Turner style monochromator. This is a single-pass monochromator, using quartz over-coated optical components. The detector is capable of operating in the range from 175-900nm.
Page 97: Visible Lamp Assembly
ProStar325 Service Manual procedures are necessary. Removal and replacement procedures are described in this section of the manual. 5.2.2 Visible lamp assembly When this option is fitted, the visible lamp mounts on top of the optics module and is held in place by two screw terminal connections.
Page 98: Optical Path
ProStar325 Service Manual Top view of optics module Grating details Grating size: 35mm x 30mm x 6mm Blaze angle: 8.5°(UV) Blaze wavelength: 250nm (UV-Vis) Lines per mm: 1200 lines/mm (UV-Vis) 5.2.4 Optical path Light coming from either source lamp is passed through a focusing lens before hitting the beam splitter.
Page 99 ProStar325 Service Manual Deuteriu m lamp Publication date 8 May 2003...
Page 100 ProStar325 Service Manual Deuterium lamp optical path Visible lamp optical path Publication date 8 May 2003...
Page 101: Removal And Replacement Procedures
ProStar325 Service Manual Removal and replacement procedures Warning Lamp surfaces and mounting brackets will become hot during operation and will remain hot for some time after being switched off. The temperatures are sufficient to cause burns. The UV lamps operate at high voltage. Contact with this voltage can cause death, serious injury, or painful electric shock.
Page 102 ProStar325 Service Manual latch push upwards • Loosen the two thumbscrews located at the front. • Holding the lamp module by these thumbscrews, carefully pull out the assembly. Publication date 8 May 2003...
Page 103: Vis Lamp Source
ProStar325 Service Manual Replacement The replacement procedure is the opposite of the removal procedure. • Carefully place the lamp assembly into position • Tighten the two thumbscrews • Re-connect the three way connector • Engage the safety latch by sliding it back down. 5.3.2 Vis lamp source Removal...
Page 104 ProStar325 Service Manual Vis lamp housing • Note that the Vis lamp does not come away with the housing. • Using a cloth to handle the lamp, gently pull it forward until it comes away from its terminations. Replacement • Holding the replacement lamp with a cloth, slide the two pins on the Vis lamp into the connector terminals as far in as possible.
Page 105: Optics Module
Optics module It is not possible to perform any optical alignment or to replace any optical components within the ProStar 325. The optics module consists of the monochromator and all other optical components. It is designed to be replaced as a module.
Page 106 ProStar325 Service Manual required for removing the Processor PWB). • On the Processor PWB, disconnect P13, P17, P22 & P15. • Carefully lift the optics module out of the detector and place safely on a flat bench. • Remove the two screws on the large silver clamp that secures the black foam padding around the optics module.
Page 107: Installing Vis Lamp Option
ProStar325 Service Manual Installing Vis lamp option The Vis lamp upgrade kit (01-107439-00) will consist of the following parts; • Vis lamp and housing • Panel cover • 2 x spacers • 2 x M4 hex cap screws • 2 x spring washes •...
Page 108 ProStar325 Service Manual Vis lamp upgrade kit • Remove the detector’s top main cover, which is held by two screws on each side. • Remove the two screws securing the small side panel and lower it down. remove Publication date 8 May 2003...
Page 109 ProStar325 Service Manual • Locate and remove the two screws at the rear of the detector used to secure the fan baffle. Then, swing the baffle to the right. screw • Remove the two screws securing the small plate adjacent to the UV lamp source.
Page 110 ProStar325 Service Manual • Remove the two padding pieces as shown below and fit the two spacers provided in the upgrade kit over the exposed screw holes. Remove these two pieces • Fit the Vis lamp housing on top of the spacers and fit the two hex cap screws that come with the upgrade kit.
Page 111: Optimizing Vis Lamp
ProStar325 Service Manual loose at this point to allow for optimization. • Remove the Power Supply assembly to enable access to the connector sitting underneath (P16). This is on the Control PWB and it is where the Vis lamp plugs into. Connect the Vis lamp and return the Power Supply assembly to its original position.
Page 112 ProStar325 Service Manual • Once communications has been established, check that the Vis lamp option is recognized. Check that the Instrument status on the Detector page of the diagnostics software displays “Vis On”. • Enter the Method tab and set the following parameters; Signal Mode: ADC counts Add Line: Time 20min Tick End of Method Line and set Method End Action to “Reset”...
Page 113: Performance Test
ProStar325 Service Manual • Fit the new panel cover that comes with the upgrade kit as shown below. • Replace and secure all other panels. Note: Once this is complete, check that the new Vis lamp is not causing any over range errors. Please refer to Diagnostics section, page 120. Performance test Execute the instrument performance (install) tests to check that the instrument meets specification...
Page 114 ProStar325 Service Manual The following tests are automatically enabled: Noise test Wavelength Accuracy (D emission only), Wavelength Reproducibility, SBW Accuracy Auto Precision Detector Linearity Refractive Index Cell Length Ratio Publication date 8 May 2003...
Page 115: Spare Parts
ProStar325 Service Manual Spare parts Location of major assemblies This section contains ordering information for spare parts. Part numbers are detailed in a list of spares provided for individual modules. Covers Part Number Description 01 107220 00 ASSY PANEL DOOR 09 101922 00 COVER LC DETECTOR BASE 01 107218 00...
Page 116: Optical Spares Parts
ProStar325 Service Manual 01 107155 00 ASSY LOOM VIS LAMP 01 107156 00 ASSY LOOM LAMP INTERCONNECT 01 107152 00 ASSY LOOM PRE-AMP CONTROL 01 107153 00 ASSY RIBBON PRE-AMP CONTROL 01 107157 00 ASSY LOOM POWER SUPPLY 01 107158 00 ASSY LOOM MAINS ASSEMBLIES Part Number...
Page 117: Upgrade Kits
FLOWCELL 4x0 SEMI MICRO Upgrade kits Part Number Description 01 107439 00 ASSY VIS LAMP FIELD UPGRADE KIT 01 107231 00 PROSTAR 325 DUAL WAVELENGTH System cables Part Number Description 01 107234 00 ETHERNET CROSSOVER (LC to PC) 01 107233 00...
Page 118 ProStar325 Service Manual Publication date 8 May 2003...
Page 119: Diagnostics
ProStar325 Service Manual Diagnostics 7.1 Introduction Fault finding on the ProStar 325 detector is made simple by several levels of diagnostic aids that are available to help diagnose problems. This allows a CSR (Customer Service Representative) to fault find the system to board level then verify system operation from the host computer.
Page 120: 7.4 Led Status Indicators
ProStar325 Service Manual Self check CRC of boot block Check CRC of FLASH main code Obtain IP address for detector 7.4 LED status indicators At power up the detector goes through an initialisation sequence to calibrate its wavelength and verify its overall operation. During this period, the LED’s on the front panel of the detector change color to indicate its current status.
Page 121 ProStar325 Service Manual All of the accessory (synchronisation and relay) outputs shall be toggled with a two second test period. These include relays 1-4, the peak-sense relay, as well as the Fault Out, Ready Out and Start Out signals. A ‘40’ will be displayed on the seven-segment LED’s Diagnostics switch position 5: * Not used at present...
Page 122: 7.6 Diagnostics Cd
7.6 Diagnostics CD 7.6.1 Introduction To run the service diagnostics program for the ProStar 325 detector, you will need to insert the Verify CD into a computer CD ROM drive and connect the pc to the detector via the Ethernet connection.
Page 123: Menu Task Bars
ProStar325 Service Manual • verify lamp operation • verify Ethernet/IrDA communications • configure TCP/IP addresses • check grating drive operation • verify all relay operations • check operation of signal processing • monitor voltage supplies • check all external connections •...
Page 124: Status Bar
ProStar325 Service Manual Just below this is the graph menu bar. It allows for; • auto scaling axis • trace zooming • cursor options • report generation • graph options • mathematics functions 7.6.4 Status bar The status bar will always appear at the bottom of the screen. It indicates the status of;...
Page 125: 7.8 Method Development
ProStar325 Service Manual Double clicking in either of these two windows will expand it to full screen viewing. 7.8 Method Development This Method Editor page is used for building methods. There are two modes of operation possible on this page. Editing & Viewing. The active mode is shown at the top of the parameters box.
Page 126: Building A Method
ProStar325 Service Manual 7.8.1 Building a method A method consists of a sequence of timed event command lines each one having been assigned a unique set of detector parameters. They can be run for a defined period of time and are totally independent of each other.
Page 127: Modifying A Method
ProStar325 Service Manual Dual wavelength selection will only be permitted if the detector is configured with the dual wavelength option. End of Method Any command line can be assigned an End of Method instruction. This is done while in editing mode and is evoked by selecting the “End of Method Line”...
Page 128: Saving A Method
ProStar325 Service Manual 7.8.4 Saving a method A method can be saved using the top menu bar by clicking on File>Save Methods As…”. From here you can allocate a method file name and store it in the desired location. 7.9 Detector Status Entering this page will display a further four sub-pages located within.
Page 129: Main
ProStar325 Service Manual 7.9.1 Main Available Instruments – “discover” button searches the Ethernet network (if the detector is configured into one) and displays all the detectors located on that network. You can then connect or disconnect to any detector configured on the network. Use the “connect”...
Page 130: Internal Method
ProStar325 Service Manual Instrument Date and Time – displays the time and date as held by the detector’s internal clock. 7.9.2 Internal Method The detector comes pre-loaded with a default method which performs a single beam absorbance scan at 254nm for single wavelength configurations.
Page 131: Alarms
ProStar325 Service Manual Run Log This will display an account of all functions performed by the detector. Error Log This will display an ongoing account of all errors encountered by the detector. These error logs can be saved in a text format by clicking on “save error log”.
Page 132: Input/Output
ProStar325 Service Manual 7.10.1 Input/Output To activate this page you must be in Diagnostic State. Go to 'Diagnostic'> 'Main' and select ‘i/o collect mode’. Then tick 'Enter Diagnostic State'. Note: you must first select i/o collect mode before entering into the diagnostic state or else this page does not become active.
Page 133: Eeprom
ProStar325 Service Manual To activate these Analog tests you must click on Start Analysis. Digital Toggles - Verify the operation of the Sync signals located at the rear of the detector. Toggle any signal by clicking in its tick box and then monitor it at the output either on the D- range connector pin, or on the Rear Panel Interface PWB if fitted.
Page 134 ProStar325 Service Manual Clicking on “Load” will display the current EEPROM information for the detector you are connected to. This can be done individually in a specific group of information, such as Lamp Details, or you can click “Load All” to display the entire contents. To make an entry into the EEPROM, enter in the desired information into the appropriate field and click on “Update”.
Page 135: Mono Options
ProStar325 Service Manual 7.10.3 Mono Options 0%T Calibration – this calibration is required whenever a lamp source or optics module is replaced. Refer to the Calibration section below. Mono Calibration - recalibrate the monochromator’s wavelengths which will load new co-efficients into the EEPROM. Refer to the Calibration section below.
Page 136 ProStar325 Service Manual Performing a Deuterium lamp emission line scan Set up the following parameters to perform a scan of D2 emission line at 656.1nm to verify that the detector’s D2 lamp, signal processing and optics are in working order. Start wavelength = 640 Stop wavelength = 670 Wave increment = 1...
Page 137: Gain And Dark Tables
ProStar325 Service Manual 7.10.4 Gain and Dark Tables Gain Table - this displays each wavelength interval where a gain calibration was performed, and shows the corresponding gain stage used at that wavelength (as described in the Electronics section). This calibration is performed using the UV lamp source which has a typical working range of 190 –...
Page 138 ProStar325 Service Manual Gain Vs. Wavelength Wavelength 190nm - 950nm Dark Values – this table displays the electronic offset voltages present in the ADC circuits on the main Processor PWB. Both the Reference and Sample channels are measured independently. These measurements are performed with the UV lamp source off. Each of the 15 gain stages is selected and any offset voltage present in the signal processing circuits is measured and tabled against its corresponding gain.
Page 139: Comms
ProStar325 Service Manual Reference Dark Measurements Gain Stages 7.10.5 Comms Publication date 8 May 2003...
Page 140 ProStar325 Service Manual IR Comms – when communicating via infra red link, use these fields to establish communication to the desired detector. The “Discover” button is greyed out. Fixed IP Settings – Used to manually assign an IP address, subnet & gateway settings. Enter the details in the desired fields then click “Set”.
Page 141: Establishing Communications
ProStar325 Service Manual 7.11 Establishing communications Go to the tab “Detector”>”Main” and then click “discover” This will display a list of all the available detectors connected to that workstation (either directly, or via a network setup). Highlight the desired detector and click on “connect”. Once communications has been established, the 'Not Ready-Lamp off' indicator will turn a red color.
Page 142: Calibration
ProStar325 Service Manual 7.12 Calibration 7.12.1 Mono A mono calibration will be required under the following circumstances; • After replacing the optics module • Wavelength accuracy test fails • After replacing the main Processor PWB • After replacing the Detector PWB Establish communications with the detector as described above.
Page 143 ProStar325 Service Manual Go to ’Diagnostic’> ’Main’ and tick ’Enter Diagnostic State’ as shown below. You must be in ‘data collect mode’. Note; you must select this mode before enabling the Diagnostic State. Publication date 8 May 2003...
Page 144 ProStar325 Service Manual Go to ’Mono Options’ and make sure that the detector is in the Diagnostic state (check the bottom of the screen and observe that there is a violet box next to the Diagnostic sign). Click on ’calibrate’ and a pop-up box “Starting Mono Calibration” will appear.
Page 145: 0% Transmission
ProStar325 Service Manual A small graph will appear indicating that mono calibration is in progress. Once completed the “Mono Calibration completed” pop- up box will appear. Press 'OK'. The wavelength calibration is now complete. Go back to 'Main' tab and un-tick “Enter Diagnostic State”. 7.12.2 0% Transmission There are three 0%T calibration options available.
Page 146 ProStar325 Service Manual Calibrate with D2 lamp on Calibrate with both lamps on These will be required under the following circumstances; Calibrate with Calibrate with Calibrate with Vis lamp on D2 lamp on both lamps on Fitting Vis lamp option Replacing Vis lamp Replacing UV lamp if Vis fitted...
Page 147 ProStar325 Service Manual Go to ’Diagnostic’> ’Mono Options’ and in the 0%T Calibration field, click on the calibration option required as per the table above. The ’Starting 0%T Calibration now’ pop up box will appear. Click ’OK’. The following information boxes will appear. Place a black piece of paper between the Flow Cell and the Surround.
Page 148 ProStar325 Service Manual A small graph will appear indicating that calibration is in progress. When the following pop-up box appears, remove the black piece of paper and click ’OK’. Another pop-up box will appear indicating that calibration is complete. Click ’OK’ to finish 0%T calibration. Publication date 8 May 2003...
Page 149 ProStar325 Service Manual Publication date 8 May 2003...

Varian ProStar 325 Service Manual

1 General Information

2 Pre-Installation

3 Installation

4 Electronics

5 Optics

6 Spare Parts

7 Diagnostics

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