Poring Pulse And Transfer Pulse - Nepagene ELEPO21 Instruction Manual

and bacteria, the output pulse will be square shape without decay, because of its high impedance value
(see lower right figure).
6.3.2

Poring Pulse and Transfer Pulse

Electroporation is the application of pulsed electric fields to a sample to create temporary pores in the
sample's cell membrane the purpose of which is to assist or eventuate the introduction of materials such
as foreign DNA into the sample's cells (-similar to electrophoresis).
The transfection efficiency and the viability of the sample varies according to the parameters of the
delivered electric pulse. Multiple high voltage pulses with a short duration and with voltage decay
followed by multiple low pulses with a long duration and with voltage decay tends to cause lower
damage of the sample and to facilitate the delivery of significantly higher amounts of DNA into the
sample's cells. The first (poring) pulses facilitate the formation of temporary pores and the second
(transferring) pulses facilitate the introduction of DNA into cells. Nepa Gene refers to the first pulse as a
"Poring Pulse" and the second pulse as a "Transfer Pulse" throughout this document.
-High Voltage
-Short Duration
For Pore Formation
with Low Damage
Poring Pulse
In Poring Pulse mode, the voltage can be set up to 3,000V, the number of pulses can be set up to 9. In
Transfer Pulse mode, the voltage can be set from up to 99.9V, the number of pulses can be set up to 20
and the polarity exchange can be set on or off.
The optimal pulse parameters vary according to the application. Please contact Nepa Gene Co., Ltd. or
your local distributor for further information.
Low Voltage Mode
Time
-Low Voltage
-Long Duration
For DNA Transfer
with Low Damage
Transfer Pulse
High Voltage Mode
Time
Time
6 Additional Information
6-5