Table of Contents
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Table of Contents
Summary of Contents for Nepagene ELEPO21
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Page 2: Important Information
The In Vitro High Voltage Electroporator ELEPO21 is an electroporation device designed to provide a convenient way to introduce materials such as foreign DNA into sample’s cells, bacteria, fungi and yeasts. -
Page 3: Safety Precautions
Safety Precautions Safety Precautions Safety Terms and Symbols Danger Disregarding instructions marked with this symbol may lead to serious injury or death. Warning Disregarding instructions marked with this symbol may lead to serious injury or property damage. Caution Disregarding instructions marked with this symbol may lead to injury or property damage. - Page 4 Safety Precautions Warning 1) Do not open the cover of the product when the power is ON. 2) Do not touch the un-insulated parts of cables and electrodes when power is on. 3) Do not operate this product in any of the following scenarios: ...
- Page 5 Safety Precautions Caution 1) Be sure to hold the connector part of the power cable when disconnecting the cable. Pulling on the cord section may cause it to fray and break. 2) Disconnect power cable from the product if the product is not in use. ...
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Page 6: Table Of Contents
Displaying Output Electric Pulse Parameters............. 5-9 Additional Information ................. 6-1 Specifications ...................... 6-1 Proper Selection of The Power Supply Cord ............6-3 ELEPO21 Novel 2-Step Multiple Pulses Regime ..........6-4 6.3.1 About pulse waveform ..............6-4 6.3.2 Poring Pulse and Transfer Pulse ............ 6-5... -
Page 7: Table Of Contents
Table of Contents... -
Page 8: Checking The Package
1 Checking the Package Checking the Package ELEPO21 Electroporator x 1 Accessories Power cord x 1 Instruction Manual (this manual) x 1... -
Page 10: Related Products
2 Related Products Related Products CU500 Cuvette Chamber The CU500 Cuvette Chamber can be connected to the ELEPO21 electroporator and is used for cell transfection with NEPA electroporation cuvettes. Electric pulses from the ELEPO21 are delivered through this product. ... - Page 11 2 Related Products...
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Page 12: Part Names
3 Part Names Part Names Front Control Panel Power Switch Voltage Output Rear RS232C port Cooling fan Power Inlet... - Page 13 3 Part Names Control Panel 1. Poring Pulse window Set Parameters frame A. Voltage [V] Display the set pulse voltage value. B. Pulse Length [msec] Display the set pulse length in milliseconds. C. Pulse Interval [msec] Display the pulse interval (duration between pulses) in milliseconds. D.
- Page 14 3 Part Names 2. Transfer Pulse window Set Parameters frame A. Voltage [V] Display the set pulse voltage value. B. Pulse Length [msec] Display the set pulse length in milliseconds. C. Pulse Interval [msec] Display the pulse interval (duration between pulses) in milliseconds. D.
- Page 15 3 Part Names 5. Button Keys A. Ω Execute the impedance measurement function between the electrodes B. Data Send the data of the set and measured parameters. C. Meas. Display the measured parameters. D. Copy Copy the set parameters to another program number. E.
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Page 16: Installation And Connecting
Installation Precautions for installation Installation site The ELEPO21 must be installed on a flat-level area. Spacing for the cooling fan Caution Be sure to leave a clearing of at least 20 cm behind the instrument for proper ventilation. - Page 17 Insert the male banana plugs of the CU500 Cuvette Chamber (optional) into the voltage output slot located on the front panel of the ELEPO21. Open the lid of the CU500 and then insert an electroporation cuvette (optional) into the insertion...
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Page 18: Operating Instructions
5 Operating Instructions Operating Instructions Overview The basic steps required to perform electroporation experiments using the ELEPO21 Electroporation System are as follows: Setting Electric Pulse Parameters 5.2.1 Display in Selection window An indicator light corresponding to the selected parameter/item lights up. Select the parameter/item by pressing ◄... -
Page 19: Parameters Of Poring Pulse Mode
5 Operating Instructions 5.2.2 Parameters of Poring Pulse mode Voltage The voltage of the first Poring Pulse (increments: 1V) Pulse Length The time duration of one Poring Pulse in milliseconds (increments: 0.1ms) Pulse Interval The time interval between Poring Pulses or the last Poring Pulse and the first Transfer Pulse in milliseconds (increments: 0.1ms) ... - Page 20 5 Operating Instructions Enter the desired value by pressing the numerical keys. If you would like to cancel an entered value, press the Stop/Clear button. Press the Enter button to save the desired value. When the value is saved, a short beep will sound twice.
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Page 21: Parameters Of Transfer Pulse Mode
5 Operating Instructions 5.2.4 Parameters of Transfer Pulse mode Voltage The voltage of the first Transfer Pulse (increments: 1V) Pulse Length The time duration of one Transfer Pulse in milliseconds (increments: 0.1ms) Pulse Interval The time interval between Transfer Pulses in milliseconds (increments: 0.1ms) ... -
Page 22: Setting Current Limit
A current limitation function is automatically built into the ELEPO21. Please ensure that the “On” selection is illuminated in the Current Limit frame when the ELEPO21 is used for In Vivo, In Utero, and In Ovo or Ex Vivo electroporation. -
Page 23: Saving, Copying And Recalling The Program
5 Operating Instructions Saving, Copying and Recalling the Program Saving the program The values displayed in the Set Parameters frame in the Poring Pulse window and the Transfer Pulse window are saved automatically on the current program number by pressing the Enter button. *How to view the current program number. - Page 24 5 Operating Instructions Enter the destination program number by pressing the numerical keys. Press the Copy button. When the copying is completed, a short beep will sound twice. Recalling the program The saved program can be recalled. Select the Memory option in the Others frame in the Selection window by pressing ◄ ▴ ▾ ▸ buttons. Press the Set button.
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Page 25: Measuring Electrical Impedance
5 Operating Instructions Press the Enter button. When the program is successfully recalled, a short beep will sound twice. Measuring Electrical Impedance Electrical impedance between the electrodes can be measured. (unit: kΩ) Press the Ω button. The value of the measured impedance will be displayed in the Impedance window. Executing Program (Delivering Electric Pulses) Press the Start button in the Button Keys Selection to execute the program. -
Page 26: Displaying Output Electric Pulse Parameters
5 Operating Instructions Danger Do not touch the exposed connections and components of electrodes and cables when power is on and electric pulses are being delivered. Displaying Output Electric Pulse Parameters Default display After delivering an electroporation program, the measured voltage and current of the first pulse and the total energy of the pulses will be displayed in the Measurements frame in the Poring Pulse window and the Transfer Pulse window. - Page 27 5 Operating Instructions Press ▸ button to display the next output Poring Pulse. This process can be repeated until the last pulse is displayed. The previous pulse can be displayed by pressing ◀ button. The total energy of the output Poring Pulses will be displayed by pressing ▸ button after displaying the last pulse.
- Page 28 5 Operating Instructions The values in the Measurements frame in the Transfer Pulse window will blink. The blinking values show the measured parameters of the first output Transfer Pulse. At the same time, “1” indicating the first pulse will be displayed on Number of Pulses option in the Set Parameter frame in the Transfer Pulse window.
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Page 30: Additional Information
6 Additional Information Additional Information Specifications If no power supply cord is provided, please select the proper power supply cord for the ELEPO21 by referring to “Specifications” and “Certified Cord” below: Warning In case you use a non-approved power supply cord for Nepa Gene products, Nepa Gene can no longer warrant the electrical safety of the device. - Page 31 6 Additional Information Number of Pulses 0-20 Polarity Switching On: With polarity switching, OFF: Without polarity switching Capacitance 2,160µF Output Measurements Voltage Poring Pulse 1,000 - 3,276V (increment: 1V) 0.0 - 999.9V (increment: 1V) Transfer Pulse 0.0 - 327.6V (increment: 0.1V) Current Poring Pulse 10.00 - 99.99A (increment: 0.01A)
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Page 32: Proper Selection Of The Power Supply Cord
6 Additional Information Proper Selection of the Power Supply Cord If no power supply cord is provided, please select the proper power supply cord for the ELEPO21 by referring to “Specifications” and “Certified Cord” below: Warning In case you use a non-approved power supply cord for Nepa Gene products, Nepa Gene can no longer warrant the electrical safety of the device. -
Page 33: Elepo21 Novel 2-Step Multiple Pulses Regime
6.3.1 About pulse waveform The ELEPO21 output pulse waveform from DC voltage is exponential-square. This ELEPO21 electroporation system makes it possible to set and apply an appropriate pulse voltage, pulse length and pulse interval for transfection into each sample. This facilitates higher transfection efficiency and higher sample viability without the need for or recourse to expensive disposable kits and special buffers. -
Page 34: Poring Pulse And Transfer Pulse
6 Additional Information and bacteria, the output pulse will be square shape without decay, because of its high impedance value (see lower right figure). Low Voltage Mode High Voltage Mode Time Time 6.3.2 Poring Pulse and Transfer Pulse Electroporation is the application of pulsed electric fields to a sample to create temporary pores in the sample’s cell membrane the purpose of which is to assist or eventuate the introduction of materials such as foreign DNA into the sample’s cells (-similar to electrophoresis). -
Page 35: Warranty
6 Additional Information Pulse parameters can be modified easily on the ELEPO21. Preliminary experiments may be needed to optimize the parameters in order to achieve both high transfection efficiency and high cell viability. To better understand the pulse parameters, please refer to the measured output values of voltage, current and energy. - Page 36 Phone: +65-6515-0110 Fax: +65-6515-0220 Taiwan Bio-East Technology 9F., No.21 Fu-Kor Rd. Shi-Lin, Taipei, 111, Taiwan Co., Ltd. Phone: +886-2-2831-3061 Fax: +886-2-2831-6374 Nepa Gene Co., Ltd. 3-1-6 Shioyaki, Ichikawa, Chiba, 272-0114, Japan Phone: +81-(0)47-306-7222 Fax: +81-(0)47-306-7333 Email: info@nepagene.jp ELEPO21 Instruction Manual 20150610...