Page 1 SP-800 Portable Water Analyzer Procedures Manual Pyxis Lab, Inc. 1729 Majestic Dr. Suite 5 Lafayette, CO 80026 USA www.pyxis-lab.com © 2019 Pyxis Lab, Inc. Pyxis Lab Proprietary and Confidential...
Page 2: Table Of Contents
Table of Contents REQUIRED REAGENTS ........................4 Turbidity ..........................12 Aluminum – Al........................16 Alkalinity, Low Range - ALKLR ....................21 Alkalinity, High Range - ALKHR ....................24 Benzotriazole/Tolyltriazole - AZOL ..................27 Bleach - BLCH ......................... 31 Bromine - Br-T ........................34 Calcium - Ca ...........................
Page 3 Manganese, High Range - MnHR ..................146 Manganese, Low Range - MnLR ..................150 Molydemum, High Range - MoHR ................... 154 Molybdenum, Low Range - MoLR ..................158 Hydrazine - N2H4 ......................162 Chloramine, Mono, Low Range - NH2C ................165 Nitrogen, Total (Test ‘N Tube Method) - N-TLR ..............
Page 4: Required Reagents
Reagent Powder 14064-99 Pillows DPD Free Chlorine CL2H Reagent Powder 14070-99 Pillows DPD Free Chlorine CL-F 21055-69 Powder Pillows DPD Free Chlorine Reagent Chlorine Dioxide 21055-69 Powder Pillows CLO2 DPD/Glycine 27709-00 Reagent Set Glycine Reagent 27621-33 Pyxis SP-800 Analyzer Procedures Manual...
Page 5 Acid Reagent Powder Pillows 2126-99 ChromaVer 3 Chromium 12066-99 Reagent Powder Pillows Total Chromium 22425-00 Chromium 1 Reagent Powder Reagent Set 2043-99 Pillows Chromium 2 Reagent Powder 2044-99 Pillows CuVer 1 Copper CuBi Reagent Powder 21058-69 Pillows Pyxis SP-800 Analyzer Procedures Manual...
Page 6 Hardness Reagent 22418-32 23199-00 Indicator Solution EDTA Solution, 1 M 22419-26 EGTA Solution 22297-26 Buffer Powder Pillows, citrate 21076-69 High Range type for Manganese MnHR Manganese 24300-00 Sodium Periodate Powder Reagent Set 21077-69 Pillows for Manganese Pyxis SP-800 Analyzer Procedures Manual...
Page 7 Reagent Powder Pillows Total Nitrogen Reagent A, Test ’N Tube HR 26719-46 Bisulfite Powder Pillows N-THR Total Nitrogen 27141-00 Reagent Set Total Nitrogen Reagent B, 26720-46 Indicator Powder Pillows Total Nitrogen Reagent C Vials, Acid Solution Pyxis SP-800 Analyzer Procedures Manual...
Page 8 P.A.N. Indicator Solution, 0.3% 21502-32 NitriVer 2 Nitrite Reagent Powder NO2HR 21075-69 Pillows NitriVer 3 Nitrite Reagent Powder NO2LR 21071-69 Pillows NitraVer 5 Nitrate Reagent Powder NO3HR 21061-69 Pillows NitraVer 5 Nitrate Reagent Powder NO3MR 21061-69 Pillows Pyxis SP-800 Analyzer Procedures Manual...
Page 9 Phosphorus Test ’N 27672-45 Sodium Hydroxide Solution, 27430-42 Tube™ Reagent Set 1.54 N Total Phosphorus Test ’N Tube™ Vials Dropper, 0.5&1.0 21247-20 mL marks Phenol Red Indicator Solution, 26575-12 spec grade Molybdovanadate PMOV Reagent 20760-32 Pyxis SP-800 Analyzer Procedures Manual...
Page 10 CLLR CLLR Reagent 31009 CLMR CLMR Reagent 31004 CL2UH CL2UH Reagent 31074 CLFTMB CLFTMB Reagent 31075 Cyclo-F Cyclo-F Reagent 31076 FeSSal FeSSal Reagent 31078 NH3-F-1 Fluorescent NH3-F 31091 NH3-F-2 Method Set NH3-F-3 NO2D Direct Reading Pyxis SP-800 Analyzer Procedures Manual...
Page 11 31080 Sb-T -3 Sb-T -4 SO3LR-1 SO3LR SO3LR Reagent Set 31089 SO3LR-2 SO3LR-3 SO3HR-1 SO3HR SO3HR Reagent Set 31090 SO3HR-2 SO3HR-3 Urea-1 Urea Urea Reagent Set 31081 Urea-2 ZnXO-1 ZnXO ZnXO Reagent Set 31052 ZnXO-2 Pyxis SP-800 Analyzer Procedures Manual...
Page 12: Turbidity
Note: Mix the sample well before transferring it to the sample vial compartment. Press the OK key in the main page. Pyxis SP-800 will start to measure the Turbidity concentration in the sample. Pyxis SP-800 will display the Turbidity concentration in NTU as turbidity.
Page 13 OK key to measure the deionized water. Figure 3 Follow the message prompts and insert the 50 NTU formazan standard into the sample vial compartment and press the OK key to measure the 50 NTU standard. Pyxis SP-800 Analyzer Procedures Manual...
Page 14 If calibration fails, the followings should be checked: • The DI blank is being contaminated. • The 50 NTU/200NTU formazan standard solution is decayed or being contaminated. • The light shield cover is not in the closing position. Pyxis SP-800 Analyzer Procedures Manual...
Page 15 6 o’clock position of the sample vial compartment or any position consistently. Pyxis SP-800 automatically turns itself off after 2 minutes with no-Key activity, except for during a measurement. Pressing and holding the OK key for 3 seconds will wake up the instrument, and return to the original page if it has any measurement data.
Page 16: Aluminum - Al
2. Aluminum – Al Test Program Description：SP-800 Aluminum Method (0.02 - 0.80 ppm Al) (Aluminon Method) Instruments and Reagents: 1. SP-800 Portable Water Analyzer 10-ml Sample Vial 3. 25-ml Sample Vial 4. 50-ml Graduated Mixing Cylinder 5. Hach Aluminum Reagent (Cat. No.22420-00) Includes: (1) AluVer 3 Aluminum Reagent Powder Pillow (Cat.
Page 17 Note: Inconsistent results will occur if any powder is undissolved. 7. Press the ZERO key. 8. Press the TMR1 Key to start the method timer, a 1-minute reaction period will begin. Invert the cylinder repeatedly for three minutes. Pyxis SP-800 Analyzer Procedures Manual...
Page 18 Note: This solution should turn a light to medium orange upon bleaching. It will not become colorless. Figure 11 14. Press the TMR3 Key to start the method timer, a 15-minute reaction period will begin. Pyxis SP-800 Analyzer Procedures Manual...
Page 19 17. Use a soft cloth or lint free paper tissue to clean the sample vial. 18. Place the prepared blank into the Pyxis SP-800 sample vial compartment and press ZERO. 19. Place the prepared sample into the Pyxis SP-800 sample vial compartment and press READ.
Page 20 3. Pyxis SP-800 automatically turns itself off after 2 minutes with no-Key activity, except for during a measurement. Pressing and holding the OK Key for 3 seconds will wake up the instrument, and return to the original page if it has any measurement data.
Page 21: Alkalinity, Low Range - Alklr
3. Alkalinity, Low Range - ALKLR Test Program Description：SP-800 Alkalinity Low Range Method (5-100 ppm as CaCO3) (Bromophenol Blue Method) Instruments and Reagents: 1. SP-800 Portable Water Analyzer 2. 10-ml Sample Vial 3. Pyxis ALK Reagent Program: 1. Press OK key (the center key) on the navigation control panel for 3 seconds until the screen lights up.
Page 22 7. Add the content of ALK -2 reagent to each vial. Cap the vials and invert to mix. 8. Press the ZERO key to zero the instrument. Pyxis SP-800 will display the page. Figure 17 9. Press the TMR1 key to start the method timer, a 1-minute reaction period will begin.
Page 23 6 o’clock position of the sample vial compartment or any position consistently. 3. Pyxis SP-800 automatically turns itself off after 2 minutes with no-Key activity, except for during a measurement. Pressing and holding the OK Key for 3 seconds will wake up the instrument, and return to the original page if it has any measurement data.
Page 24: Alkalinity, High Range - Alkhr
4. Alkalinity, High Range - ALKHR Test Program Description：SP-800 Alkalinity High Range Method (100-500 ppm as CaCO3) (Bromophenol Blue Method) Instruments and Reagents: 1. SP-800 Portable Water Analyzer 2. 10-ml Sample Vial 3. Pyxis ALK Reagent Program: 1. Press OK key (the center key) on the navigation control panel for 3 seconds until the screen lights up.
Page 25 7. Add the content of ALK -2 reagent to each vial. Cap the vials and invert to mix. 8. Press the ZERO key to zero the instrument. Pyxis SP-800 will display the page. Figure 22 9. Press the TMR1 key to start the method timer, a 1-minute reaction period will begin.
Page 26 6 o’clock position of the sample vial compartment or any position consistently. 3. Pyxis SP-800 automatically turns itself off after 2 minutes with no-Key activity, except for during a measurement. Pressing and holding the OK Key for 3 seconds will wake up the instrument, and return to the original page if it has any measurement data.
Page 27: Benzotriazole/Tolyltriazole - Azol
5. Benzotriazole/Tolyltriazole - AZOL Test Program Description：SP-800 AZOL Method (0.7-16.0 ppm BENZO or TOLY) (UV Photolysis Method) Instruments and Reagents: 1. SP-800 Portable Water Analyzer 2. 10-ml Sample Vial 3. Ultraviolet (UV) lamp, 115V, 60HZ 4. UV Safety Goggles 5. 25-ml Sample Vial 6.
Page 28 Position the cursor to COLOR icon by navigation keys and press the OK key to enter COLOR selection interface, position the cursor to AZOL icon. Figure 25 Press the OK key to enter AZOL test program interface. Figure 26 Press the ZERO key. Figure 27 Pyxis SP-800 Analyzer Procedures Manual...
Page 29 6 o’clock position of the sample vial compartment or any position consistently. 3. Pyxis SP-800 automatically turns itself off after 2 minutes with no-Key activity, except for during a measurement. Pressing and holding the OK key for 3 seconds will wake up the instrument, and return to the original page if it has any measurement data.
Page 30 Press the FORM key to select a concentration form from the list of forms (BENZO or TOLY). Press the UNIT key to select a concentration unit among the list of ppb, ppm, mg/L, and ug/L. Pyxis SP-800 Analyzer Procedures Manual...
Page 31: Bleach - Blch
6. Bleach - BLCH Test Program Description：SP-800 Bleach Method (0.50-16.0 percent) (Direct Reading Method) Instruments and Reagents: SP-800 Portable Water Analyzer 10-ml Sample Vial Program: Press OK key (the center Key) on the navigation control panel for 3 seconds until the screen lights up. The main page will display eight major feature groups.
Page 32 Note: Analyze samples immediately after collection. Use a soft cloth or lint free paper tissue to clean the sample vial. Place the prepared sample into the Pyxis SP-800 sample vial compartment and press READ. Concentration value based on the last absorbance value measured will be calculated and displayed.
Page 33 6 o’clock position of the sample vial compartment or any position consistently. Pyxis SP-800 automatically turns itself off after 2 minutes with no-key activity, except for during a measurement. Pressing and holding the OK key for 3 seconds will wake up the instrument and return to the original page if it has any measurement data.
Page 34: Bromine - Br-T
7. Bromine - Br-T Test Program Description：SP-800 Br-T Method (0.04-4.50 ppm Br2) (DPD Method) Instruments and Reagents: SP-800 Portable Water Analyzer 10-ml Sample Vial HACH DPD Total Chlorine Reagent Powder Pillows (Cat. No. 21056-69) Program: Press OK key (the center key) on the navigation control panel for 3 seconds until the screen lights up.
Page 35 Place sample vial back into the sample vial compartment and Press the TMR1 key to start the method timer, a 3-minute reaction period will begin. Pyxis SP-800 will start to monitor the reaction between the reagent and the species you want to measure in the water sample. The concentration is shown in the chart as a function of time.
Page 36 6 o’clock position of the sample vial compartment or any position consistently. Pyxis SP-800 automatically turns itself off after 2 minutes with no-key activity, except for during a measurement. Pressing and holding the OK key for 3 seconds will wake up the instrument and return to the original page if it has any measurement data.
Page 37: Calcium - Ca
8. Calcium - Ca Test Program Description：SP-800 Calcium Method (0.08-4.0 ppm Ca as CaCO3) (Calmagite Colorimetric Method) Instruments and Reagents: 1. SP-800 Portable Water Analyzer 2. 10-ml Sample Vial (SP-800 accessory, 10 ml sample vial) 3. 100-ml graduated mixing cylinder 4.
Page 38 (the blank sample). Stopper. Swirl the vial to mix the reagent. Use a soft cloth or lint free paper tissue to clean the sample vial. Place the prepared blank into the Pyxis SP-800 sample vial compartment and press ZERO to zero the instrument. Pyxis SP-800 will display the page.
Page 39 6 o’clock position of the sample vial compartment or any position consistently. 3. Pyxis SP-800 automatically turns itself off after 2 minutes with no-Key activity, except for during a measurement. Pressing and holding the OK Key for 3 seconds will wake up the instrument, and return to the original page if it has any measurement data.
Page 40 Press the FORM key to select a concentration form from the list of forms (CaCO3 or Ca). Press the UNIT key to select a concentration unit among the list of ppb, ppm, mg/L, and ug/L. Pyxis SP-800 Analyzer Procedures Manual...
Page 41: Calcium Hardness - Cahr
9. Calcium Hardness - CaHR Test Program Description：SP-800 Calcium Hardness (25-500 ppm Ca as CaCO3) (Murexide Method) Instruments and Reagents: 1. SP-800 Portable Water Analyzer 2. 10-ml Sample Vial 3. Pyxis CaHR Reagent Program: 1. Press OK key (the center key) on the navigation control panel for 3 seconds until the screen lights up.
Page 42 ZERO. 11. Use a soft cloth or lint free paper tissue to clean the sample vial. 12. Place the prepared blank into the Pyxis SP-800 sample vial compartment and press ZERO. 13. Place the prepared sample into the Pyxis SP-800 sample vial compartment and press READ.
Page 43 6 o’clock position of the sample vial compartment or any position consistently. 3. Pyxis SP-800 automatically turns itself off after 2 minutes with no-Key activity, except for during a measurement. Pressing and holding the OK Key for 3 seconds will wake up the instrument, and return to the original page if it has any measurement data.
Page 44: Oxygen Demand, Chemical (Reactor Digestion 2H Method) - Cod
10. Oxygen Demand, Chemical (Reactor Digestion 2h Method) - COD Test Program Description：SP-800 COD Method (4-150 ppm/30-1500 ppm/30-15000 COD) (Reactor Digestion Method) Instruments and Reagents: SP-800 Portable Water Analyzer Pyxis RD-800 Reactor Blender, 120 V, 14 speed/ Blender, 240 V, 14 speed...
Page 45 Note: The vial will become very hot during mixing. Prepare a blank by repeating Steps 4 to 7, substituting 2.00 ml (0.2 ml for the 0 to 15,000 mg/L range) deionized water for the sample. Note: Be sure the pipet is clean. Pyxis SP-800 Analyzer Procedures Manual...
Page 46 Press the OK key to enter CODLR test program interface. Figure 51 Insert the COD/TNT adapter into the vial holder by rotating the adapter until it drops into place. Then push down to fully insert it. Pyxis SP-800 Analyzer Procedures Manual...
Page 47 Tightly cover the vial with the instrument cap. Note: The blank is stable when stored in the dark. press the ZERO key to zero the instrument. Pyxis SP-800 will display the page. Figure 52 Clean the outside of the sample vial with a towel.
Page 48 Note: Do not move the vial from side to side as this can cause errors. Tightly cover the vial with the instrument cap. Note: The blank is stable when stored in the dark. press the ZERO key to zero the instrument. Pyxis SP-800 will display the page. Pyxis SP-800 Analyzer Procedures Manual...
Page 49 10. Tightly cover the vial with the instrument cap and press the READ key. 11. Concentration value based on the last absorbance value measured will be calculated and displayed. Pyxis SP-800 will display the page. Figure 57 Note: When using High Range Plus COD Digestion Reagent Vials, multiply the reading by 10.
Page 50 Pyxis SP-800 automatically turns itself off after 2 minutes with no-Key activity, except for during a measurement. Pressing and holding the OK key for 3 seconds will wake up the instrument, and return to the original page if it has any measurement data.
Page 51: Oxygen Demand, Chemical (Reactor Digestion 20 Minutes Method) - Cod
11. Oxygen Demand, Chemical (Reactor Digestion 20 Minutes Method) - COD Test Program Description：SP-800 COD Method (15-150 ppm/100-1500 ppmCOD) (Reactor Digestion 20 Minutes Method) Instruments and Reagents: SP-800 Portable Water Analyzer Pyxis RD-800 Reactor Blender, 120 V, 14 speed/ Blender, 240 V, 14 speed...
Page 52 Note: If a pure green color appears in the reacted sample, measure the COD and, if necessary, repeat the test with a diluted sample. 12. Use one of the following analytical techniques to measure the COD: Pyxis SP-800 Analyzer Procedures Manual...
Page 53 Note: Do not move the vial from side to side as this can cause errors. Tightly cover the vial with the instrument cap. Note: The blank is stable when stored in the dark. press the ZERO key to zero the instrument. Pyxis SP-800 will display the page. Pyxis SP-800 Analyzer Procedures Manual...
Page 54 10. Tightly cover the vial with the instrument cap and press the READ key. 11. Concentration value based on the last absorbance value measured will be calculated and displayed. Figure 62 Colorimetric Determination, 100 to 1500 mg/L COD Position the cursor to CODHF icon. Figure 63 Pyxis SP-800 Analyzer Procedures Manual...
Page 55 Tightly cover the vial with the instrument cap. Note: The blank is stable when stored in the dark. press the ZERO key to zero the instrument. Pyxis SP-800 will display the page. Figure 65 Clean the outside of the sample vial with a towel.
Page 56 6 o’clock position of the sample vial compartment or any position consistently. Pyxis SP-800 automatically turns itself off after 2 minutes with no-Key activity, except for during a measurement. Pressing and holding the OK key for 3 seconds will wake up the instrument, and return to the original page if it has any measurement data.
Page 57: Chloride Medium Range - Cllr
12. Chloride Medium Range - CLLR Test Program Description：SP-800 Chlorine Medium Range Method (2.5-40.0 ppm CL) (Turbidimetric Method) Instruments and Reagents: 1. SP-800 Portable Water Analyzer 2. 10-ml Sample Vial 3. Pyxis CLLR Reagent Program: 1. Press OK key (the center key) on the navigation control panel for 3 seconds until the screen lights up.
Page 58 4. Fill a sample vial to the 10-ml line with sample (the blank sample). 5. Use a soft cloth or lint free paper tissue to clean the sample vial. 6. Place the prepared blank into the Pyxis SP-800 sample vial compartment and press the ZERO key. Pyxis SP-800 will display the page.
Page 59 6 o’clock position of the sample vial compartment or any position consistently. 4. Pyxis SP-800 automatically turns itself off after 2 minutes with no-key activity, except for during a measurement. Pressing and holding the OK key for 3 seconds will wake up the instrument, and return to the original page if it has any measurement data.
Page 60: Chloride Medium Range - Clmr
13. Chloride Medium Range - CLMR Test Program Description：SP-800 Chlorine Medium Range Method (40-400 ppm CL) (Turbidimetric Method) Instruments and Reagents: 1. SP-800 Portable Water Analyzer 2. 10-ml Sample Vial 3. Pyxis CLMR Reagent Program: 1. Press OK key (the center key) on the navigation control panel for 3 seconds until the screen lights up.
Page 61 4. Fill a sample vial to the 10-ml line with CLMR reagent (the blank sample). 5. Use a soft cloth or lint free paper tissue to clean the sample vial. 6. Place the prepared blank into the Pyxis SP-800 sample vial compartment and press the ZERO key. Pyxis SP-800 will display the page.
Page 62 6 o’clock position of the sample vial compartment or any position consistently. 4. Pyxis SP-800 automatically turns itself off after 2 minutes with no-key activity, except for during a measurement. Pressing and holding the OK key for 3 seconds will wake up the instrument, and return to the original page if it has any measurement data.
Page 63: Chlorine, Total, High Range - Cl2H
14. Chlorine, Total, High Range - CL2H Test Program Description：SP-800 Chlorine Total High Range Method (0.1-10 ppm CL2) (DPD Method) Instruments and Reagents: 1. SP-800 Portable Water Analyzer 2. 10-ml Sample Vial 3. HACH DPD Total Chlorine Reagent Powder Pillows (Cat. No. 14064-99) Program: 1.
Page 64 4. Fill a sample vial to the 10-ml line with sample (the blank sample). 5. Use a soft cloth or lint free paper tissue to clean the sample vial. 6. Place the prepared blank into the Pyxis SP-800 sample vial compartment and press the ZERO key. Pyxis SP-800 will display the page.
Page 65 6 o’clock position of the sample vial compartment or any position consistently. 4. Pyxis SP-800 automatically turns itself off after 2 minutes with no-key activity, except for during a measurement. Pressing and holding the OK key for 3 seconds will wake up the instrument, and return to the original page if it has any measurement data.
Page 66: Chlorine, Free, High Range - Cl2H
15. Chlorine, Free, High Range - CL2H Test Program Description：SP-800 Chlorine Free High Range Method (0.1-10 ppm CL2) (DPD Method) Instruments and Reagents: 1. SP-800 Portable Water Analyzer 2. 10-ml Sample Vial 3. HACH DPD Free Chlorine Reagent Powder Pillows (Cat. No. 14070-99) Program: 1.
Page 67 4. Fill a sample vial to the 10-ml line with sample (the blank sample). 5. Use a soft cloth or lint free paper tissue to clean the sample vial. 6. Place the prepared blank into the Pyxis SP-800 sample vial compartment and press the ZERO key. Pyxis SP-800 will display the page.
Page 68 6 o’clock position of the sample vial compartment or any position consistently. 4. Pyxis SP-800 automatically turns itself off after 2 minutes with no-key activity, except for during a measurement. Pressing and holding the OK key for 3 seconds will wake up the instrument, and return to the original page if it has any measurement data.
Page 69: Chlorine, Free, Ultra-High Range - Cl2Uh
16. Chlorine, Free, Ultra-High Range - CL2UH Test Program Description：SP-800 Chlorine Free Ultra-High Range Method (5-400 ppm CL2) (Iodimetr Method) Instruments and Reagents: 1. SP-800 Portable Water Analyzer 2. 10-ml Sample Vial 3. Pyxis CL2UH Reagent Program: 1. Press OK key (the center key) on the navigation control panel for 3 seconds until the screen lights up.
Page 70 4. Fill a sample vial to the 10-ml line with sample (the blank sample). 5. Use a soft cloth or lint free paper tissue to clean the sample vial. 6. Place the prepared blank into the Pyxis SP-800 sample vial compartment and press the ZERO key. Pyxis SP-800 will display the page.
Page 71 6 o’clock position of the sample vial compartment or any position consistently. 4. Pyxis SP-800 automatically turns itself off after 2 minutes with no-key activity, except for during a measurement. Pressing and holding the OK key for 3 seconds will wake up the instrument, and return to the original page if it has any measurement data.
Page 72: Chlorine, Free - Cl-F
17. Chlorine, Free - CL-F Test Program Description：SP-800 CL-F Method (0.02-2.20 ppm CL2) (DPD Method) Instruments and Reagents: 1. SP-800 Portable Water Analyzer 2. 10-ml Sample Vial 3. HACH DPD Free Chlorine Powder Pillows (Cat. No. 21055-69) Program: 1. Press OK key (the center key) on the navigation control panel for 3 seconds until the screen lights up.
Page 73 7. Place sample vial back into the sample vial compartment and Press the TMR1 key to start the method timer, a 1-minute reaction period will begin. 8. Pyxis SP-800 will start to monitor the reaction between the reagent and the species you want to measure in the water sample. The concentration is shown in the chart as a function of time.
Page 74 6 o’clock position of the sample vial compartment or any position consistently. 4. Pyxis SP-800 automatically turns itself off after 2 minutes with no-key activity, except for during a measurement. Pressing and holding the OK key for 3 seconds will wake up the instrument, and return to the original page if it has any measurement data.
Page 75: Chlorine, Free - Cltmb
18. Chlorine, Free - CLTMB Test Program Description：SP-800 Free Chlorine Method (0.02-1.20 ppm CL2) (TMB Method) Instruments and Reagents: 1. SP-800 Portable Water Analyzer 2. 10-ml Sample Vial 3. Pyxis CLTMB Reagent Program: 1. Press OK key (the center key) on the navigation control panel for 3 seconds until the screen lights up.
Page 76 7. Place sample vial back into the sample vial compartment and Press the TMR1 key to start the method timer, a 1-minute reaction period will begin. 8. Pyxis SP-800 will start to monitor the reaction between the reagent and the species you want to measure in the water sample. The concentration is shown in the chart as a function of time.
Page 77 6 o’clock position of the sample vial compartment or any position consistently. 4. Pyxis SP-800 automatically turns itself off after 2 minutes with no-key activity, except for during a measurement. Pressing and holding the OK key for 3 seconds will wake up the instrument, and return to the original page if it has any measurement data.
Page 78: Chlorine Dioxide - Clo2
19. Chlorine Dioxide - CLO2 Test Program Description：SP-800 Chlorine, Dioxide Method, (0.04-5 ppm CLO2) (DPD Method) Instruments and Reagents: 1. SP-800 Portable Water Analyzer 2. 10-ml Sample Vial 3. HACH Chlorine Dioxide DPD/Glycine Reagent Set (Cat. No. 27709-00) Includes one of each: (1) DPD Free Chlorine Reagent Powder Pillows (Cat.
Page 79 5. Use a soft cloth or lint free paper tissue to clean the sample vial. Place the prepared blank into the Pyxis SP-800 sample vial compartment and press the ZERO key to zero the instrument. Pyxis SP-800 will display the page.
Page 80 6 o’clock position of the sample vial compartment or any position consistently. 4. Pyxis SP-800 automatically turns itself off after 2 minutes with no-key activity, except for during a measurement. Pressing and holding the OK key for 3 seconds will wake up the instrument, and return to the original page if it has any measurement data.
Page 81 5. Press the CONF key in the method result page to launch the method setup and calibration page. Press the UNIT key to select a concentration unit among the list of ppb, ppm, mg/L, and ug/L. Pyxis SP-800 Analyzer Procedures Manual...
Page 82: Chlorine Dioxide - Clo2D
20. Chlorine Dioxide - CLO2D Test Program Description：SP-800 Chlorine Dioxide Method (7.3-50.0 ppm CLO2) (Direct Reading Method) Instruments and Reagents: 1. SP-800 Portable Water Analyzer 2. 10-ml Sample Vial Program: 1. Press OK key (the center Key) on the navigation control panel for 3 seconds until the screen lights up.
Page 83 5. Use a soft cloth or lint free paper tissue to clean the sample vial. Place the prepared blank into the Pyxis SP-800 sample vial compartment and press the ZERO key to zero the instrument. Pyxis SP-800 will display the page.
Page 84 6 o’clock position of the sample vial compartment or any position consistently. 4. Pyxis SP-800 automatically turns itself off after 2 minutes with no-key activity, except for during a measurement. Pressing and holding the OK key for 3 seconds will wake up the instrument, and return to the original page if it has any measurement data.
Page 85: Chlorine, Total - Cl-T
21. Chlorine, Total - CL-T Test Program Description：SP-800 CL-T Method (0.02-2.20 ppm CL2) (DPD Method) Instruments and Reagents: 1. SP-800 Portable Water Analyzer 2. 10-ml Sample Vial 3. HACH DPD Total Chlorine Reagent Powder Pillows (Cat. No. 21056-69) Program: 1. Press OK key (the center key) on the navigation control panel for 3 seconds until the screen lights up.
Page 86 7. Place sample vial back into the sample vial compartment and Press the TMR1 key to start the method timer, a 3-minute reaction period will begin. 8. Pyxis SP-800 will start to monitor the reaction between the reagent and the species you want to measure in the water sample. The concentration is shown in...
Page 87 6 o’clock position of the sample vial compartment or any position consistently. 4. Pyxis SP-800 automatically turns itself off after 2 minutes with no-key activity, except for during a measurement. Pressing and holding the OK key for 3 seconds will wake up the instrument, and return to the original page if it has any measurement data.
Page 88: Cyanide - Cn
22. Cyanide - CN Test Program Description：SP-800 Cyanide Method (0.008 – 0.240 ppm CN) (Pyridine-Pyrazalone Method) Instruments and Reagents: 1. SP-800 Portable Water Analyzer 2. 10-ml Sample Vial 3. HACH Cyanide Reagent Set (Cat. No. 24302-00) Includes: (1) CyaniVer 3 Cyanide Reagent Powder Pillows (Cat. No. 21068-69) (2) CyaniVer 4 Cyanide Reagent Powder Pillows (Cat.
Page 89 5. Add the contents of one CyaniVer 3 Cyanide Reagent Powder Pillow to the sample vial, Cap the vial and invert repeatedly to mix. 6. Press the ZERO key. Pyxis SP-800 will display the page. Figure 120 7. Press the TMR1 key to start the method timer, a 30-second reaction period will begin.
Page 90 14. Press the TMR3 key to start the method timer, a 30-minute reaction period will begin. Note: If cyanide is present, a pink color will develop which then turns blue after a few minutes. Figure 122 Pyxis SP-800 Analyzer Procedures Manual...
Page 91 16. Fill another sample vial to the 10-ml line with sample (the blank sample). 17. Use a soft cloth or lint free paper tissue to clean the sample vial. 18. Repeat step 2, place the prepared blank into the Pyxis SP-800 sample vial compartment and press the ZERO key.
Page 92 5. Press the CONF key in the method result page to launch the method setup and calibration page. Press the UNIT key to select a concentration unit among the list of ppb, ppm, mg/L, and ug/L. Pyxis SP-800 Analyzer Procedures Manual...
Page 93: Color, True And Apparent - Color
23. Color, True and Apparent - COLOR Test Program Description：SP-800 Chromium Hexavalent Method (25-500 units) (APHA Platinum-Cobalt Standard Method) Instruments and Reagents: 1. SP-800 Portable Water Analyzer 2. 10-ml Sample Vial 3. Aspirator, vacuum 4. Filter Holder, 47 mm, 300 ml graduated 5.
Page 94 10. Use a soft cloth or lint free paper tissue to clean the sample vial. Place the prepared blank into the Pyxis SP-800 sample vial compartment and press the ZERO key to zero the instrument. Pyxis SP-800 will display the page.
Page 95 Figure 127 11. Place the prepared sample into the Pyxis SP-800 sample vial compartment and press the READ key. 12. Concentration value based on the last absorbance value measured will be calculated and displayed. Figure 128 13. Press EXIT key to return to the main page.
Page 96 Pyxis SP-800 Analyzer Procedures Manual...
Page 97: Chromium, Hexavalent - Cr6
24. Chromium, Hexavalent - Cr6 Test Program Description：SP-800 Chromium Hexavalent Method (0.01-0.60 ppm Cr6+) (1,5- Diphenylcarbohydrazide Method) Instruments and Reagents: 1. SP-800 Portable Water Analyzer 2. 10-ml Sample Vial 3. HACH ChromaVer 3 Chromium Reagent Powder Pillows Program: 1. Press OK key (the center key) on the navigation control panel for 3 seconds until the screen lights up.
Page 98 7. Place sample vial back into the sample vial compartment and Press the TMR1 key to start the method timer, a 5-minute reaction period will begin. 8. Pyxis SP-800 will start to monitor the reaction between the reagent and the species you want to measure in the water sample. The concentration is shown in the chart as a function of time.
Page 99 6 o’clock position of the sample vial compartment or any position consistently. 4. Pyxis SP-800 automatically turns itself off after 2 minutes with no-key activity, except for during a measurement. Pressing and holding the OK key for 3 seconds will wake up the instrument, and return to the original page if it has any measurement data.
Page 100: Chromium, Total - Crt
25. Chromium, Total - CrT Test Program Description：SP-800 Chromium, Total Method (0.01-0.60 ppm Cr6) (Alkaline Hypobromite Oxidation Method) Instruments and Reagents: 1. SP-800 Portable Water Analyzer 2. 10-ml Sample Vial 3. 25-ml Graduated Mixing Cylinder 4. HACH Total Chromium Reagent (Cat. No. 22425-00) Includes: Acid Reagent Powder Pillows (Cat.
Page 101 Cap the vial and invert repeatedly to mix. Remove the cap. 6. Place the prepared sample into a boiling water bath. 7. Press the ZERO key. Pyxis SP-800 will display the page. Figure 137 8. Press the TMR1 key to start the method timer, a 5-minute reaction period will begin.
Page 102 18. Use a soft cloth or lint free paper tissue to clean the sample vial. 19. Repeat step 2, place the prepared blank into the Pyxis SP-800 sample vial compartment and press the ZERO key. Pyxis SP-800 Analyzer...
Page 103 20. Place the prepared sample into the Pyxis SP-800 sample vial compartment and press the READ key. Concentration value based on the last absorbance value measured will be calculated and displayed. Figure 139 21. Press EXIT key to return to the main page.
Page 104: Copper - Cubi
26. Copper - CuBi Test Program Description：SP-800 Copper Method (0.02-5.00 ppm Cu) (Bicinchoninate Method) Instruments and Reagents: 1. SP-800 Portable Water Analyzer 2. 10-ml Sample Vial 3. HACH CuVer 1 Copper Reagent Powder Pillows (Cat. No. 21058-69) Program: 1. Press OK key (the center key) on the navigation control panel for 3 seconds until the screen lights up.
Page 105 2-minute reaction period will begin. Note: Accuracy is not affected by undissolved powder. 8. Pyxis SP-800 will start to monitor the reaction between the reagent and the species you want to measure in the water sample. The concentration is shown in the chart as a function of time 9.
Page 106 6 o’clock position of the sample vial compartment or any position consistently. 4. Pyxis SP-800 automatically turns itself off after 2 minutes with no-key activity, except for during a measurement. Pressing and holding the OK key for 3 seconds will wake up the instrument, and return to the original page if it has any measurement data.
Page 107: Copper - Culr
27. Copper - CuLR Test Program Description：SP-800 Copper Low Range Method (0.006-0.2 ppm Cu) (Porphyrin Method) Instruments and Reagents: 1. SP-800 Portable Water Analyzer 2. 10-ml Sample Vial 3. HACH Copper Reagent Includes: Copper Masking Reagent Powder Pillows (Cat. No. 26034-49) Porphyrin 1 Reagent Powder Pillows (Cat.
Page 108 Cap the vial and invert to dissolve. Note: The yellow color will turn blue momentarily. If any copper is present, the yellow color will return. 8. Press the ZERO key. Pyxis SP-800 will display the page. Pyxis SP-800 Analyzer Procedures Manual...
Page 109 EXIT key. Press the OK key to the icon menu-assisted. 11. Use a soft cloth or lint free paper tissue to clean the sample vial. 12. Repeat step 2, place the prepared blank into the Pyxis SP-800 sample vial compartment and press the ZERO key.
Page 110 6 o’clock position of the sample vial compartment or any position consistently. 4. Pyxis SP-800 automatically turns itself off after 2 minutes with no-key activity, except for during a measurement. Pressing and holding the OK key for 3 seconds will wake up the instrument, and return to the original page if it has any measurement data.
Page 111: Cyanuric Acid - Cyan
28. Cyanuric Acid - CYAN Test Program Description：SP-800 Cyanuric Acid Method (7.0-55.0 ppm CYAN) (Turbidimetric Method) Instruments and Reagents: 1. SP-800 Portable Water Analyzer 2. 10-ml Sample Vial 3. HACH Cyanuric Acid 2 Reagent Powder Pillow Program: 1. Press OK key (the center key) on the navigation control panel for 3 seconds until the screen lights up.
Page 112 Note: Filtering is required for highly turbid samples. 5. Use a soft cloth or lint free paper tissue to clean the sample vial. 6. Place the prepared blank into the Pyxis SP-800 sample vial compartment and press the ZERO key. Pyxis SP-800 will display the page.
Page 113 6 o’clock position of the sample vial compartment or any position consistently. 4. Pyxis SP-800 automatically turns itself off after 2 minutes with no-key activity, except for during a measurement. Pressing and holding the OK key for 3 seconds will wake up the instrument, and return to the original page if it has any measurement data.
Page 114: Diethyl Hydroxylamine - Deha
29. Diethyl hydroxylamine - DEHA Test Program Description：SP-800 DEHA Method (0.009-0.500 ppm DEHA) (Iron Reduction Method for Oxygen Scavengers) Instruments and Reagents: SP-800 Portable Water Analyzer 10-ml Sample Vial 25ml Sample Vial HACH Copper Reagent Set (Cat. No. 24466-00) Includes: DEHA Reagent 1 Powder Pillow (Cat.
Page 115 Add exactly 0.5 ml of DEHA Reagent 2 Solution to each sample vial. Cap and swirl to mix. Place both sample vials in the dark. Note: A purple color will slowly develop if DEHA is present. Press the ZERO key. Pyxis SP-800 will display the page. Pyxis SP-800 Analyzer Procedures Manual...
Page 116 12. Pour 10 ml solution out of the 25-ml prepared sample vial (the prepared sample). 13. Use a soft cloth or lint free paper tissue to clean the 10 ml sample vial. 14. Repeat step 2, place the prepared blank into the Pyxis SP-800 sample vial compartment and press the ZERO key.
Page 117 6 o’clock position of the sample vial compartment or any position consistently. Pyxis SP-800 automatically turns itself off after 2 minutes with no-key activity, except for during a measurement. Pressing and holding the OK key for 3 seconds will wake up the instrument, and return to the original page if it has any measurement data.
Page 118: Fluoride - F
30. Fluoride - F Test Program Description：SP-800 Fluoride Method (0.05-2.00 ppm F) (SPADNS Method) Instruments and Reagents: 1. SP-800 Portable Water Analyzer 2. 10-ml Sample Vial 3. HACH SPADNS Reagent for Fluoride Program: 1. Press OK key (the center key) on the navigation control panel for 3 seconds until the screen lights up.
Page 119 10. Use a soft cloth or lint free paper tissue to clean the sample vial. 11. Place the prepared blank into the Pyxis SP-800 sample vial compartment. Repeat step 2, place the prepared blank into the Pyxis SP-800 sample vial compartment and press the ZERO Key.
Page 120 12. Place the prepared sample into the Pyxis SP-800 sample vial compartment and press the READ key. 13. A new concentration value based on the last absorbance value measured will be calculated and displayed. Figure 164 14. Press EXIT Key to return to the main page.
Page 121: Tannin And Lignin - Ligs
31. Tannin and Lignin – Ligs Test Program Description：SP-800 Tannin and Lignin Method (0.10 – 9.00 ppm Tannins (as Tannic Acid)) (Tyrosine Method) Instruments and Reagents: 1. SP-800 Portable Water Analyzer 2. 10-ml Sample Vial 3. Cylinder, graduated mixing, 25-mL 4.
Page 122 Note: A blue color will develop if tannins and/or lignins are present. 8. Pour 10 mL of each solution into two sample cells. 9. Press the ZERO key. 10. Press the TMR1 Key to start the method timer, a 25-minute reaction period will begin. Pyxis SP-800 Analyzer Procedures Manual...
Page 123 ZERO. 12. Use a soft cloth or lint free paper tissue to clean the sample vial. 13. Place the prepared blank (10 ml) into the Pyxis SP-800 sample vial compartment and press ZERO. 14. Place the prepared sample (10 ml) into the Pyxis SP-800 sample vial compartment and press READ.
Page 124 3. Pyxis SP-800 automatically turns itself off after 2 minutes with no-Key activity, except for during a measurement. Pressing and holding the OK Key for 3 seconds will wake up the instrument, and return to the original page if it has any measurement data.
Page 125: Total Iron - Femo
32. Total Iron - FeMo Test Program Description：SP-800 Total Iron Method (0.03-1.80 ppm Fe) (Ferro MO Method) Instruments and Reagents: 1. SP-800 Portable Water Analyzer 2. 10-ml Sample Vial 3. 25-ml Sample Vial 4. 50-ml Graduated Mixing Cylinder 5. HACH FerroMo Reagent Set Includes: FerroMo Iron Reagent 1 Powder Pillows (Cat.
Page 126 7. Add the contents of one FerroMo Iron Reagent 2 Powder Pillow to 25-ml sample vial, Cap the vial and shake for 30 seconds. 8. Press the ZERO key. Pyxis SP-800 will display the page. Pyxis SP-800 Analyzer Procedures Manual...
Page 127 13. Use a soft cloth or lint free paper tissue to clean the sample vial. 14. Place the prepared blank into the Pyxis SP-800 sample vial compartment. Repeat step 2, place the prepared blank into the Pyxis SP-800 sample vial compartment and press the ZERO key.
Page 128 6 o’clock position of the sample vial compartment or any position consistently. 3. Pyxis SP-800 automatically turns itself off after 2 minutes with no-Key activity, except for during a measurement. Pressing and holding the OK key for 3 seconds will wake up the instrument, and return to the original page if it has any measurement data.
Page 129: Total Iron - Feph
33. Total Iron - FePh Test Program Description：SP-800 Iron Method (0.03-3.00 ppm Fe) (1,10 phenanthroline Method) Instruments and Reagents: 1. SP-800 Portable Water Analyzer 2. 10-ml Sample Vial 3. HACH FerroVer Iron Reagent Powder Pillows Program: 1. Press OK key (the center key) on the navigation control panel for 3 seconds until the screen lights up.
Page 130 Note: Samples containing visible rust should be allowed to react at least five minutes. 8. Pyxis SP-800 will start to monitor the reaction between the reagent and the species you want to measure in the water sample. The concentration is shown in...
Page 131 6 o’clock position of the sample vial compartment or any position consistently. 4. Pyxis SP-800 automatically turns itself off after 2 minutes with no-key activity, except for during a measurement. Pressing and holding the OK key for 3 seconds will wake up the instrument, and return to the original page if it has any measurement data.
Page 132: Total Iron - Fesal
34. Total Iron - FeSal Test Program Description：SP-800 Total Iron Method (0.05-5.0 ppm Fe) (5-Sulfosalicylic Acid Dihydrate Method) Instruments and Reagents: 1. SP-800 Portable Water Analyzer 2. 10-ml Sample Vial 3. Pyxis FeSal Reagent Program: 1. Press OK key (the center key) on the navigation control panel for 3 seconds until the screen lights up.
Page 133 8. Place sample vial back into the sample vial compartment and Press the TMR1 key to start the method timer, a 10-minute reaction period will begin. 9. Pyxis SP-800 will start to monitor the reaction between the reagent and the species you want to measure in the water sample. The concentration is shown in the chart as a function of time 10.
Page 134 6 o’clock position of the sample vial compartment or any position consistently. 4. Pyxis SP-800 automatically turns itself off after 2 minutes with no-key activity, except for during a measurement. Pressing and holding the OK key for 3 seconds will wake up the instrument, and return to the original page if it has any measurement data.
Page 135: Total Iron - Fetp
35. Total Iron - FeTp Test Program Description：SP-800 Iron Method (0.04-1.80 ppm Fe) (TPTZ Method) Instruments and Reagents: 1. SP-800 Portable Water Analyzer 2. 10-ml Sample Vial 3. HACH TPTZ Iron Reagent Powder Pillows (Cat. No. 26087-99) Program: 1. Press OK key (the center key) on the navigation control panel for 3 seconds until the screen lights up.
Page 136 7. Place sample vial back into the sample vial compartment and Press the TMR1 key to start the method timer, a 3-minute reaction period will begin. 8. Pyxis SP-800 will start to monitor the reaction between the reagent and the species you want to measure in the water sample. The concentration is shown in the chart as a function of time.
Page 137 6 o’clock position of the sample vial compartment or any position consistently. 4. Pyxis SP-800 automatically turns itself off after 2 minutes with no-key activity, except for during a measurement. Pressing and holding the OK key for 3 seconds will wake up the instrument, and return to the original page if it has any measurement data.
Page 138: Total Iron - Fezi
36. Total Iron - FeZi Test Program Description：SP-800 Total Iron Method (0.011-1.300 ppm Fe) (Ferrozine Method) Instruments and Reagents: 1. SP-800 Portable Water Analyzer 2. 10-ml Sample Vial 3. 25-ml Sample Vial 4. HACH FerroZine Iron Reagent Solution Pillows (Cat. No. 2301-66) Program: 1.
Page 139 6. Add the contents of the contents of one FerroZine Iron Reagent Solution Pillow to 25-ml sample vial, Swirl the vial to mix the reagent. 7. Press the ZERO key. Pyxis SP-800 will display the page. Figure 193 8. Press the TMR1 key to start the method timer, a 5-minute reaction period will begin.
Page 140 11. Use a soft cloth or lint free paper tissue to clean the sample vial. 12. Place the prepared blank into the Pyxis SP-800 sample vial compartment. Repeat step 2, place the prepared blank into the Pyxis SP-800 sample vial compartment and press the ZERO key.
Page 141 6 o’clock position of the sample vial compartment or any position consistently. 3. Pyxis SP-800 automatically turns itself off after 2 minutes with no-Key activity, except for during a measurement. Pressing and holding the OK key for 3 seconds will wake up the instrument, and return to the original page if it has any measurement data.
Page 142: Magnesium - Mg
37. Magnesium - Mg Test Program Description：SP-800 Magnesium Method (0.13-4.00 ppm Mg as CaCO3) (Calmagite Colorimetric Method) Instruments and Reagents: 1. SP-800 Portable Water Analyzer 2. 10-ml Sample Vial 3. 100-ml graduated mixing cylinder 5. HACH Hardness Reagent Set (Cat. No. 23199-00) Includes: Alkali Solution for Calcium and Magnesium Test (Cat.
Page 143 10. Use a soft cloth or lint free paper tissue to clean the sample vial. Place the prepared blank into the Pyxis SP-800 sample vial compartment and press the ZERO Key to zero the instrument. Pyxis SP-800 will display the page.
Page 144 Figure 199 11. Place the prepared sample into the Pyxis SP-800 sample vial compartment and press the READ Key. 12. Concentration value based on the last absorbance value measured will be calculated and displayed. Figure 200 13. Press EXIT Key to return to the main page.
Page 145 Press the FORM key to select a concentration form from the list of forms (CaCO3 or Mg). Press the UNIT key to select a concentration unit among the list of ppb, ppm, mg/L, and ug/L. Pyxis SP-800 Analyzer Procedures Manual...
Page 146: Manganese, High Range - Mnhr
38. Manganese, High Range - MnHR Test Program Description：SP-800 Manganese High Range Method (0.2-20.0 ppm Mn) (Periodate Oxidation Method) Instruments and Reagents: 1. SP-800 Portable Water Analyzer 2. 10-ml Sample Vial 3. HACH High Range Manganese Reagent Set (Cat. No. 24300-00) Includes: Buffer Powder Pillows, citrate type for Manganese (Cat.
Page 147 Note: Adjust the pH to 4 to 5 with 5.0 N sodium hydroxide before analysis. 5. Use a soft cloth or lint free paper tissue to clean the sample vial. 6. Place the prepared blank into the Pyxis SP-800 sample vial compartment and press the ZERO key. Pyxis SP-800 will display the page.
Page 148 2-minute reaction period will begin. Note: A violet color will form if manganese is present. 10. Pyxis SP-800 will start to monitor the reaction between the reagent and the species you want to measure in the water sample. The concentration is shown in the chart as a function of time 11.
Page 149 5. Press the CONF key in the method result page to launch the method setup and calibration page. Press the UNIT key to select a concentration unit among the list of ppb, ppm, mg/L, and ug/L. Pyxis SP-800 Analyzer Procedures Manual...
Page 150: Manganese, Low Range - Mnlr
39. Manganese, Low Range - MnLR Test Program Description：SP-800 Manganese Low Range Method (0.02-0.70 ppm Mn) (PAN Method) Instruments and Reagents: 1. SP-800 Portable Water Analyzer 2. 10-ml Sample Vial 3. HACH Manganese Reagent Set (Cat. No. 26517-00) Includes: Alkaline-Cyanide Reagent (Cat. No. 21223-26) Ascorbic Acid Powder Pillows (Cat.
Page 151 Note: An orange color will develop in the sample if manganese is present. Note: A pipet may be used to dispense 0.4 ml of the PAN Indicator Solution. 9. Press the ZERO key. Pyxis SP-800 will display the page. Pyxis SP-800 Analyzer...
Page 152 EXIT key. Press the OK key to the icon menu-assisted. 12. Use a soft cloth or lint free paper tissue to clean the sample vial. 13. Repeat step 2, place the prepared blank into the Pyxis SP-800 sample vial compartment and press the ZERO key.
Page 153 6 o’clock position of the sample vial compartment or any position consistently. 4. Pyxis SP-800 automatically turns itself off after 2 minutes with no-key activity, except for during a measurement. Pressing and holding the OK key for 3 seconds will wake up the instrument, and return to the original page if it has any measurement data.
Page 154: Molydemum, High Range - Mohr
40. Molydemum, High Range - MoHR Test Program Description：SP-800 Molybdenum High Range Method (0.2-40.0 ppm Mo6) (Mercaptoacetic Acid Method) Instruments and Reagents: 1. SP-800 Portable Water Analyzer 2. 10-ml Sample Vial 3. HACH Molybdenum Reagent Set (Cat. No. 26041-00) Includes: (1) MolyVer 1 Reagent Powder Pillows (Cat.
Page 155 Note: Filter turbid samples. 5. Use a soft cloth or lint free paper tissue to clean the sample vial. 6. Place the prepared blank into the Pyxis SP-800 sample vial compartment and press the ZERO key. 7. Take the sample vial out, add the contents of one MolyVer 1 Reagent Powder Pillow to the sample vial, Swirl the vial to mix the reagent.
Page 156 Figure 214 11. Pyxis SP-800 will start to monitor the reaction between the reagent and the species you want to measure in the water sample. The concentration is shown in the chart as a function of time 12. When the timer reaches the preset time and the reaction is complete, the value of concentration will be shown on the page.
Page 157 4. Pyxis SP-800 automatically turns itself off after 2 minutes with no-key activity, except for during a measurement. Pressing and holding the OK key for 3 seconds will wake up the instrument, and return to the original page if it has any measurement data.
Page 158: Molybdenum, Low Range - Molr
41. Molybdenum, Low Range - MoLR Test Program Description：SP-800 Molydemum Low Range Method (0.07-3.00 ppm Mo6) (Ternary Complex Method) Instruments and Reagents: 1. SP-800 Portable Water Analyzer 2. 10-ml Sample Vial 3. 25-ml mixing graduated cylinder 4. HACH Molybdenum Reagent Set, 20 mL sample (Cat. No. 24494-00) Includes: Molybdenum 1 Reagent for 20 mL sample size (Cat.
Page 159 7. Use a soft cloth or lint free paper tissue to clean the sample vial. 8. Place the prepared blank into the Pyxis SP-800 sample vial compartment and press the ZERO key to zero the instrument. Pyxis SP-800 will display the page. Figure 219...
Page 160 10. Place sample vial back into the sample vial compartment and Press the TMR1 key to start the method timer, a 2-minute reaction period will begin. 11. Pyxis SP-800 will start to monitor the reaction between the reagent and the species you want to measure in the water sample. The concentration is shown in the chart as a function of time 12.
Page 161 4. Press the CONF key in the method result page to launch the method setup and calibration page. Press the UNIT key to select a concentration unit among the list of ppb, ppm, mg/L, and ug/L. Pyxis SP-800 Analyzer Procedures Manual...
Page 162: Hydrazine - N2H4
42. Hydrazine - N2H4 Test Program Description：SP-800 Hydrazine Method (0.016-0.5 ppm N2H4) (p-Dimethylaminobenzaldehyde Method) Instruments and Reagents: 1. SP-800 Portable Water Analyzer 2. 10-ml Sample Vial 3. HACH HydraVer 2 Hydrazine Reagent Program: 1. Press OK key (the center key) on the navigation control panel for 3 seconds until the screen lights up.
Page 163 EXIT key. Press the OK key to the icon menu-assisted. 10. Use a soft cloth or lint free paper tissue to clean the sample vial. 11. Repeat step 2, place the prepared blank into the Pyxis SP-800 sample vial compartment and press the ZERO key.
Page 164 6 o’clock position of the sample vial compartment or any position consistently. 4. Pyxis SP-800 automatically turns itself off after 2 minutes with no-key activity, except for during a measurement. Pressing and holding the OK key for 3 seconds will wake up the instrument, and return to the original page if it has any measurement data.
Page 165: Chloramine, Mono, Low Range - Nh2C
43. Chloramine, Mono, Low Range - NH2C Test Program Description：SP-800 Chloramine, Mono, Low Range Method (0.1-3.0 ppm CL2) (Indophenol Method) Instruments and Reagents: 1. SP-800 Portable Water Analyzer 2. 10-ml Sample Vial 3. HACH Monochlor F Reagent Pillows (Cat. No. 28022-46) Program: 1.
Page 166 6-minute reaction period will begin. Note: The color development time depends on the sample temperature. 9. Pyxis SP-800 will start to monitor the reaction between the reagent and the species you want to measure in the water sample. The concentration is shown in the chart as a function of time 10.
Page 167 6 o’clock position of the sample vial compartment or any position consistently. 4. Pyxis SP-800 automatically turns itself off after 2 minutes with no-key activity, except for during a measurement. Pressing and holding the OK key for 3 seconds will wake up the instrument, and return to the original page if it has any measurement data.
Page 168: Nitrogen, Total (Test 'N Tube Method) - N-Tlr
44. Nitrogen, Total (Test ‘N Tube Method) - N-TLR Test Program Description：SP-800 N-TLR Method (0.5-25.0 ppm N) (Test ‘N Tube Method) Instruments and Reagents: SP-800 Portable Water Analyzer Pyxis RD-800 Reactor COD/TNT adapter HACH Test ’N Tube Total Nitrogen Reagent Set (Cat. No. 26722-45) Includes: TN Reagent C Vials, Acid Solution*(Cat.
Page 169 Note: For increased performance, a diffuser band covers the light path holes on the adapter. Do not remove the diffuser band Press the ZERO key. Pyxis SP-800 will display the page. Figure 233 10. Press the TMR1 key to start the method timer, a 3-minute reaction period will begin.
Page 170 11. When the timer reaches the preset time and the reaction is complete, after the timer beeps, Pyxis SP-800 will display the page. Figure 234 12. Remove the caps and add one TN Reagent B Powder Pillow to each vial. Cap the vials and shake for 15 seconds.
Page 171 Note: Do not move the vial from side to side as this can cause errors. 21. Repeat step 5, place the prepared blank into the Pyxis SP-800 sample vial compartment and press the ZERO key.
Page 172 Press the FORM key to select a concentration form from the list of forms (N or NH3). Press the UNIT key to select a concentration unit among the list of ppb, ppm, mg/L, and ug/L. Pyxis SP-800 Analyzer Procedures Manual...
Page 173: Nitrogen, Total (Test 'N Tube Method) - N-Thr
45. Nitrogen, Total (Test ‘N Tube Method) - N-THR Test Program Description：SP-800 N-THR Method (10-150 ppm N) (Test ‘N Tube Method) Instruments and Reagents: SP-800 Portable Water Analyzer Pyxis RD-800 Reactor COD/TNT adapter Test ’N Tube HR Total Nitrogen Reagent Set (Cat. No. 27141-00) Includes: HR Total Nitrogen Hydroxide Digestion Vials (Cat.
Page 174 Note: For increased performance, a diffuser band covers the light path holes on the adapter. Do not remove the diffuser band Press the ZERO key. Pyxis SP-800 will display the page. Figure 239 10. Press the TMR1 key to start the method timer, a 3-minute reaction period will...
Page 175 11. When the timer reaches the preset time and the reaction is complete, Pyxis SP- 800 will display the page. Figure 240 12. After the timer beeps, remove the caps and add one Total Nitrogen Reagent B Powder Pillow to each vial. Cap the vials and shake for 15 seconds.
Page 176 20. Repeat step 5, place the Total Nitrogen Reagent C vial containing the reagent blank into the Pyxis SP-800 sample vial compartment and press the ZERO key. 21. Place the Total Nitrogen Reagent C vial containing the reagent sample into the Pyxis SP-800 sample vial compartment and press the READ key.
Page 177 Press the FORM key to select a concentration form from the list of forms (N or NH3). Press the UNIT key to select a concentration unit among the list of ppb, ppm, mg/L, and ug/L. Pyxis SP-800 Analyzer Procedures Manual...
Page 178: Nitrogen, Ammonia - Nh3S
46. Nitrogen, Ammonia - NH3S Test Program Description：SP-800 Nitrogen, Ammonia Method (0.02-0.5 ppm NH3S-N) (Salicylate Method) Instruments and Reagents: 1. SP-800 Portable Water Analyzer 2. 10-ml Sample Vial 3. HACH Ammonia Nitrogen Reagent Set for 10-mL samples (Cat. No. 26680-00) Includes: Ammonia Cyanurate Reagent Powder Pillows (Cat.
Page 179 Add the contents of one Ammonia Salicylate Reagent Powder Pillow to each sample vial. Cap the vials and invert to mix. Press the ZERO key. Pyxis SP-800 will display the page. Figure 246 Press the TMR1 key to start the method timer, a 3-minute reaction period will begin.
Page 180 13. Use a soft cloth or lint free paper tissue to clean the sample vial. 14. Repeat step 2, place the prepared blank into the Pyxis SP-800 sample vial compartment and press the ZERO key. 15. Place the prepared sample into the Pyxis SP-800 sample vial compartment and press the READ key.
Page 181 6 o’clock position of the sample vial compartment or any position consistently. 4. Pyxis SP-800 automatically turns itself off after 2 minutes with no-key activity, except for during a measurement. Pressing and holding the OK key for 3 seconds will wake up the instrument, and return to the original page if it has any measurement data.
Page 182: Nitrogen, Ammonia (Test 'N Tube) - Nh3Lr
47. Nitrogen, Ammonia (Test ‘N Tube) - NH3LR Test Program Description：SP-800 Nitrogen, Ammonia, Low Range Method (0.08–2.50 ppm NH3-N) (Salicylate Method) Instruments and Reagents: 1. SP-800 Portable Water Analyzer 2. 10-ml Sample Vial 3. COD/TNT adapter 4. HACH AmVer Reagent Set for Nitrogen, Ammonia, Low Range TNT (Cat. No.
Page 183 Note: A green color will develop if ammonia is present. Insert the COD/TNT adapter into the vial holder by rotating the adapter until it drops into place. Then push down to fully insert it. Press the ZERO key. Pyxis SP-800 will display the page. Pyxis SP-800 Analyzer Procedures Manual...
Page 184 Press the OK key to the icon menu-assisted. 12. Use a soft cloth or lint free paper tissue to clean the sample vial. 13. Repeat step 2, place the prepared blank into the Pyxis SP-800 sample vial compartment and press the ZERO key.
Page 185 6 o’clock position of the sample vial compartment or any position consistently. 4. Pyxis SP-800 automatically turns itself off after 2 minutes with no-key activity, except for during a measurement. Pressing and holding the OK key for 3 seconds will wake up the instrument, and return to the original page if it has any measurement data.
Page 186: Nitrogen, Ammonia (Test 'N Tube) - Nh3Hr
48. Nitrogen, Ammonia (Test ‘N Tube) - NH3HR Test Program Description：SP-800 Nitrogen, Ammonia, High Range Method (1.0-50.0 ppm NH3-N) (Salicylate Method) Instruments and Reagents: 1. SP-800 Portable Water Analyzer 2. 10-ml Sample Vial 3. COD/TNT adapter 4. HACH AmVer™ Reagent Set for Nitrogen, Ammonia, High Range, TN (Cat. No.
Page 187 Note: A green color will develop if ammonia is present. Insert the COD/TNT adapter into the vial holder by rotating the adapter until it drops into place. Then push down to fully insert it. Press the ZERO key. Pyxis SP-800 will display the page. Pyxis SP-800 Analyzer Procedures Manual...
Page 188 Press the OK key to the icon menu-assisted. 12. Use a soft cloth or lint free paper tissue to clean the sample vial. 13. Repeat step 2, place the prepared blank into the Pyxis SP-800 sample vial compartment and press the ZERO key.
Page 189 6 o’clock position of the sample vial compartment or any position consistently. 4. Pyxis SP-800 automatically turns itself off after 2 minutes with no-key activity, except for during a measurement. Pressing and holding the OK key for 3 seconds will wake up the instrument, and return to the original page if it has any measurement data.
Page 190: Nickel - Ni
49. Nickel - Ni Test Program Description：SP-800 Nickel Method (0-1.00 ppm Ni) (PAN Method) Instruments and Reagents: 1. SP-800 Portable Water Analyzer 2. 10-ml Sample Vial 3. 25-ml Sample Vial 4. HACH Nickel Reagent Set, 25 mL sample Includes: EDTA Reagent Powder Pillows (Cat. No. 7005-99) Phthalate-Phosphate Reagent Powder Pillows (Cat.
Page 191 Step 6. 7. Add 1.0 ml of 0.3% PAN Indicator Solution to each vial. Invert several times to mix. Note: Use the plastic dropper provided. 8. Press the ZERO key. Pyxis SP-800 will display the page. Pyxis SP-800 Analyzer Procedures Manual...
Page 192 14. Use a soft cloth or lint free paper tissue to clean the sample vial. 15. Place the prepared blank into the Pyxis SP-800 sample vial compartment. Repeat step 2, place the prepared blank into the Pyxis SP-800 sample vial compartment and press the ZERO key.
Page 193 6 o’clock position of the sample vial compartment or any position consistently. 3. Pyxis SP-800 automatically turns itself off after 2 minutes with no-Key activity, except for during a measurement. Pressing and holding the OK key for 3 seconds will wake up the instrument, and return to the original page if it has any measurement data.
Page 194: Nitrogen Dioxide - No2D
50. Nitrogen Dioxide - NO2D Test Program Description：SP-800 Nitrogen Dioxide Method (100-1000 ppm NO2) (Direct Reading Method) Instruments and Reagents: SP-800 Portable Water Analyzer 10-ml Sample Vial Program: Press OK key (the center Key) on the navigation control panel for 3 seconds until the screen lights up.
Page 195 Use a soft cloth or lint free paper tissue to clean the sample vial. Place the prepared blank into the Pyxis SP-800 sample vial compartment and press the ZERO key to zero the instrument. Pyxis SP-800 will display the page.
Page 196 6 o’clock position of the sample vial compartment or any position consistently. Pyxis SP-800 automatically turns itself off after 2 minutes with no-key activity, except for during a measurement. Pressing and holding the OK key for 3 seconds will wake up the instrument, and return to the original page if it has any measurement data.
Page 197: Nitrite, High Rang - No2Hr
51. Nitrite, High Rang - NO2HR Test Program Description：SP-800 Nitrite High Range Method (2.0-150.0 ppm NO2) (Ferrous Sulfate Method) Instruments and Reagents: 1. SP-800 Portable Water Analyzer 2. 10-ml Sample Vial 3. HACH NitriVer 2 Nitrite Reagent Powder Pillows Pyxis Program: 1.
Page 198 7. Place sample vial back into the sample vial compartment and Press the TMR1 key to start the method timer, a 10-minute reaction period will begin. 8. Pyxis SP-800 will start to monitor the reaction between the reagent and the species you want to measure in the water sample. The concentration is shown in the chart as a function of time 9.
Page 199 6 o’clock position of the sample vial compartment or any position consistently. 4. Pyxis SP-800 automatically turns itself off after 2 minutes with no-key activity, except for during a measurement. Pressing and holding the OK key for 3 seconds will wake up the instrument, and return to the original page if it has any measurement data.
Page 200: Nitrite, Low Rang - No2Lr
52. Nitrite, Low Rang - NO2LR Test Program Description：SP-800 Nitrite Low Range Method (0.005-0.350 ppm NO2) (Diazotization Method) Instruments and Reagents: 1. SP-800 Portable Water Analyzer 2. 10-ml Sample Vial 3. HACH NitriVer 3 Nitrite Reagent Powder Pillows Program: 1. Press OK key (the center key) on the navigation control panel for 3 seconds until the screen lights up.
Page 201 7. Place sample vial back into the sample vial compartment and Press the TMR1 key to start the method timer, a 15-minute reaction period will begin. 8. Pyxis SP-800 will start to monitor the reaction between the reagent and the species you want to measure in the water sample. The concentration is shown in the chart as a function of time 9.
Page 202 6 o’clock position of the sample vial compartment or any position consistently. 4. Pyxis SP-800 automatically turns itself off after 2 minutes with no-key activity, except for during a measurement. Pressing and holding the OK key for 3 seconds will wake up the instrument, and return to the original page if it has any measurement data.
Page 203: Nitrate, High Range - No3Hr
53. Nitrate, High Range - NO3HR Test Program Description：SP-800 Nitrate High Range Method (0.8-30.0 ppm N) (Cadmium Reduction Method) Instruments and Reagents: 1. SP-800 Portable Water Analyzer 2. 10-ml Sample Vial 3. HACH NitraVer 5 Nitrate Reagent Powder (Cat. No.21061-69) Program: 1.
Page 204 5. Use a soft cloth or lint free paper tissue to clean the sample vial. Place the prepared blank into the Pyxis SP-800 sample vial compartment and press the ZERO key to zero the instrument. Pyxis SP-800 will display the page.
Page 205 Figure 283 9. Pyxis SP-800 will start to monitor the reaction between the reagent and the species you want to measure in the water sample. The concentration is shown in the chart as a function of time 10. When the timer reaches the preset time and the reaction is complete, the value of concentration will be shown on the page.
Page 206 Press the FORM key to select a concentration form from the list of forms (NO3 or N). Press the UNIT key to select a concentration unit among the list of ppb, ppm, mg/L, and ug/L. Pyxis SP-800 Analyzer Procedures Manual...
Page 207: Nitrate, Mid-Range - No3Mr
54. Nitrate, Mid-Range - NO3MR Test Program Description：SP-800 Nitrate Mid-Range Method (0.2-5.0 ppm N) (Cadmium Reduction Method) Instruments and Reagents: 1. SP-800 Portable Water Analyzer 2. 10-ml Sample Vial 3. HACH NitraVer 5 Nitrate Reagent Powder Pillows (Cat. No. 21061-69) Program 1.
Page 208 5. Use a soft cloth or lint free paper tissue to clean the sample vial. Place the prepared blank into the Pyxis SP-800 sample vial compartment and press the ZERO key to zero the instrument. Pyxis SP-800 will display the page.
Page 209 Figure 289 9. Pyxis SP-800 will start to monitor the reaction between the reagent and the species you want to measure in the water sample. The concentration is shown in the chart as a function of time 10. When the timer reaches the preset time and the reaction is complete, the value of concentration will be shown on the page.
Page 210 Press the FORM key to select a concentration form from the list of forms (NO3 or N). Press the UNIT key to select a concentration unit among the list of ppb, ppm, mg/L, and ug/L. Pyxis SP-800 Analyzer Procedures Manual...
Page 211: Nitrate, High Range (Test 'N Tube Method) - No3Ca
55. Nitrate, High Range (Test ‘N Tube Method) - NO3CA Test Program Description：SP-800 NO3CA Method (0.3-30.0 ppm NO3-N) (Chronotropic Acid Method) Instruments and Reagents: SP-800 Portable Water Analyzer COD/TNT adapter HACH NitraVer Χ Nitrate, High Range Test ‘N Tube Reagent Set (Cat. No. 26053- Includes: (1) Nitrate Pretreatment Solution Vials (Cat.
Page 212 COLOR selection interface, position the cursor to NO3CA icon. Figure 292 Press the ZERO key to zero the instrument. Pyxis SP-800 will display the page. Figure 293 10. Remove the cap from the vial. Using a funnel, add the contents of one NitraVer X Reagent B Powder Pillow to the vial.
Page 213 13. Pyxis SP-800 will start to monitor the reaction between the reagent and the species you want to measure in the water sample. The concentration is shown in the chart as a function of time. 14. When the timer reaches the preset time and the reaction is complete, the value of concentration will be shown on the page.
Page 214: Peroxyacetic - Paa
56. Peroxyacetic - PAA Test Program Description：SP-800 Peroxyacetic Method (25.0-500.0 ppm PAA) (Iodimetry Method) Instruments and Reagents: SP-800 Portable Water Analyzer 10-ml Sample Vial Pyxis PAA Reagent Program: Press OK key (the center key) on the navigation control panel for 3 seconds until the screen lights up.
Page 215 Use a soft cloth or lint free paper tissue to clean the sample vial. Place the prepared blank into the Pyxis SP-800 sample vial compartment and press the ZERO key to zero the instrument. Pyxis SP-800 will display the page. Figure 298 Take the sample vial out and add the PAA reagent to the sample vial (the prepared sample), Cap the sample vial.
Page 216 Pyxis SP-800 will start to monitor the reaction between the reagent and the species you want to measure in the water sample. The concentration is shown in the chart as a function of time 10. When the timer reaches the preset time and the reaction is complete, the value of concentration will be shown on the page.
Page 217: Phosphorus, Reactive - Opo4
57. Phosphorus, Reactive - OPO4 Test Program Description：SP-800 Orthophosphate Method (0.05-2.50 ppm PO4) (Molybdenum Blue Method) Instruments and Reagents: 1. SP-800 Portable Water Analyzer 2. 10-ml Sample Vial 3. HACH PhosVer 3 Phosphate Reagent Powder Pillows (Cat. No. 21060-69) Program: 1.
Page 218 7. Place sample vial back into the sample vial compartment and Press the TMR1 key to start the method timer, a 2-minute reaction period will begin. 8. Pyxis SP-800 will start to monitor the reaction between the reagent and the species you want to measure in the water sample. The concentration is shown in the chart as a function of time 9.
Page 219 6 o’clock position of the sample vial compartment or any position consistently. 4. Pyxis SP-800 automatically turns itself off after 2 minutes with no-Key activity, except for during a measurement. Pressing and holding the OK key for 3 seconds will wake up the instrument, and return to the original page if it has any measurement data.
Page 220: Phosphonates - Orgp
58. Phosphonates - OrgP Test Program Description：SP-800 Phosphonates Method (0.05-2.50 ppm PO4) (Persulfate UV Oxidation Method) Instruments and Reagents: 1. SP-800 Portable Water Analyzer 2. 10-ml Sample Vial 3. Ultraviolet (UV) lamp,115V,60HZ 4. UV safety goggles 5. 25-ml sample Vial 6.
Page 221 COLOR selection interface, position the cursor to OrgP icon. Figure 307 8. Press the OK key to enter OrgP test program interface. Figure 308 9. Press the ZERO key. Pyxis SP-800 will display the page. Pyxis SP-800 Analyzer Procedures Manual...
Page 222 Figure 310 16. When the timer reaches the preset time and the reaction is complete, after the timer beeps, the cursor will automatically switch to EXIT key. Press the OK key to the icon menu-assisted. Pyxis SP-800 Analyzer Procedures Manual...
Page 223 18. Insert the COD/TNT adapter into the vial holder by rotating the adapter until it drops into place. Then push down to fully insert it. 19. Repeat step 7, place the prepared blank into the Pyxis SP-800 sample vial compartment and press the ZERO key.
Page 224 6 o’clock position of the sample vial compartment or any position consistently. 3. Pyxis SP-800 automatically turns itself off after 2 minutes with no-Key activity, except for during a measurement. Pressing and holding the OK key for 3 seconds will wake up the instrument, and return to the original page if it has any measurement data.
Page 225: Phosphorus, Reactive - Pami
59. Phosphorus, Reactive - Pami Test Program Description：SP-800 Phosphorus, Reactive Method (0.2-30.0 ppm PO4) (Amino Acid Method) Instruments and Reagents: 1. SP-800 Portable Water Analyzer 2. 10-ml Sample Vial 3. HACH High Range Reactive Phosphorus Reagent Set (Cat. No. 22441-00) Includes: Amino Acid Reagent (Cat.
Page 226 8. Place sample vial back into the sample vial compartment and Press the TMR1 key to start the method timer, a 10-minute reaction period will begin. 9. Pyxis SP-800 will start to monitor the reaction between the reagent and the species you want to measure in the water sample. The concentration is shown in the chart as a function of time 10.
Page 227 6 o’clock position of the sample vial compartment or any position consistently. 4. Pyxis SP-800 automatically turns itself off after 2 minutes with no-key activity, except for during a measurement. Pressing and holding the OK key for 3 seconds will wake up the instrument, and return to the original page if it has any measurement data.
Page 228: Phosphorus, Total (Test 'N Tube Method) - P-Tlr
60. Phosphorus, Total (Test ‘N Tube Method) - P-TLR Test Program Description：SP-800 P-TLR Method (0.06-3.50 ppm PO4) (Test ‘N Tube Method) Instruments and Reagents: SP-800 Portable Water Analyzer Pyxis RD-800 Reactor COD/TNT adapter HACH Total Phosphorus Test ’N Tube Reagent Set (Cat. No. 27426-45) Includes: PhosVer 3 Phosphate Reagent Powder Pillows (Cat.
Page 229 COLOR selection interface, position the cursor to P-TLR icon. Figure 318 13. Press the ZERO key to zero the instrument. Pyxis SP-800 will display the page. Figure 319 14. Remove the cap from the vial. Using a funnel, add the contents of one PhosVer 3 Phosphate Reagent Powder Pillow to the vial.
Page 230 17. Pyxis SP-800 will start to monitor the reaction between the reagent and the species you want to measure in the water sample. The concentration is shown in the chart as a function of time 18. When the timer reaches the preset time and the reaction is complete, the value of concentration will be shown on the page.
Page 231: Phosphorus, Total (Test 'N Tube Method) - P-Thr
61. Phosphorus, Total (Test ‘N Tube Method) - P-THR Test Program Description：SP-800 P-THR Method (1.0-100.0 ppm PO4) (Test ‘N Tube Method) Instruments and Reagents: SP-800 Portable Water Analyzer Pyxis RD-800 Reactor COD/TNT adapter HACH Total High Range Phosphorus Test ’N Tube™ Reagent Set (Cat. No.
Page 232 COLOR selection interface, position the cursor to P-THR icon. Figure 322 14. Press the ZERO key. Pyxis SP-800 will display the page. Figure 323 15. Press the TMR1 key to start the method timer, a 7-minute reaction period will begin.
Page 233 17. Repeat step 5, place the blank into the Pyxis SP-800 sample vial compartment and press the ZERO key. 18. Place the prepared sample into the Pyxis SP-800 sample vial compartment and press the READ key.
Page 234: Potential Of Hydrogen - Ph
62. Potential of Hydrogen - pH Test Program Description：SP-800 Potential of Hydrogen Method (6.5-8.5) (Colorimetric PH Determination Using Phenol Red) Instruments and Reagents: 1. SP-800 Portable Water Analyzer 2. 10-ml Sample Vial 3. HACH Phenol Red Indicator Solution, spec grade (Cat. No. 26575-12) 4.
Page 235 5. Use a soft cloth or lint free paper tissue to clean the sample vial. Place the prepared blank into the Pyxis SP-800 sample vial compartment and press the ZERO key to zero the instrument. Pyxis SP-800 will display the page.
Page 236 6 o’clock position of the sample vial compartment or any position consistently. 4. Pyxis SP-800 automatically turns itself off after 2 minutes with no-key activity, except for during a measurement. Pressing and holding the OK key for 3 seconds will wake up the instrument, and return to the original page if it has any measurement data.
Page 237: Phosphorus, Reactive - Pmov
63. Phosphorus, Reactive - PMoV Test Program Description：SP-800 Phosphorus, Reactive Method (0.2-45.0 ppm PO4) (Molybdovanadate Method) Instruments and Reagents: 1. SP-800 Portable Water Analyzer 2. 10-ml Sample Vial 3. Pyxis PMoV Reagent Program: Press OK key (the center key) on the navigation control panel for 3 seconds until the screen lights up.
Page 238 Note: A yellow color will form if phosphate is present. A small amount of yellow will be present in the blank, because of the reagent. Press the ZERO key to zero the instrument. Pyxis SP-800 will display the page. Figure 333 Press the TMR1 key to start the method timer, a 5-minute reaction period will begin.
Page 239 6 o’clock position of the sample vial compartment or any position consistently. 4. Pyxis SP-800 automatically turns itself off after 2 minutes with no-key activity, except for during a measurement. Pressing and holding the OK key for 3 seconds will wake up the instrument, and return to the original page if it has any measurement data.
Page 240: Polymer - Poly
64. Polymer - POLY Test Program Description：SP-800 Polymer Method (2.0-13.0 ppm PAA) (Turbidimetric Method) Instruments and Reagents: 1. SP-800 Portable Water Analyzer 2. 10-ml Sample Vial 3. Pyxis POLY Reagent Program: 1. Press OK key (the center key) on the navigation control panel for 3 seconds until the screen lights up.
Page 241 10. Use a soft cloth or lint free paper tissue to clean the sample vial. 11. Place the prepared blank into the Pyxis SP-800 sample vial compartment. Repeat step 2, place the prepared blank into the Pyxis SP-800 sample vial compartment and press the ZERO key.
Page 242 6 o’clock position of the sample vial compartment or any position consistently. 4. Pyxis SP-800 automatically turns itself off after 2 minutes with no-key activity, except for during a measurement. Pressing and holding the OK key for 3 seconds will wake up the instrument, and return to the original page if it has any measurement data.
Page 243: Antimony Trivalent - Sb3
65. Antimony Trivalent - Sb3+ Test Program Description：SP-800 Antimony Trivalent Method (0.01-0.10 ppm Sb ) (PADAP Method) Instruments and Reagents: 1. SP-800 Portable Water Analyzer 2. 10-ml Sample Vial 3. 100-ml Graduated Cylinder 4. Separatory Funnel 5. Pyxis Sb Reagent Program: 1.
Page 244: Use A Soft Cloth Or Lint Free Paper Tissue To Clean The Sample Vial. Place The Prepared Blank Into The Pyxis Sp-800 Sample Vial Compartment And Press The
12. Use a soft cloth or lint free paper tissue to clean the sample vial. Place the prepared blank into the Pyxis SP-800 sample vial compartment and press the ZERO key to zero the instrument. Pyxis SP-800 will display the page.
Page 245 6 o’clock position of the sample vial compartment or any position consistently. 4. Pyxis SP-800 automatically turns itself off after 2 minutes with no-key activity, except for during a measurement. Pressing and holding the OK key for 3 seconds will wake up the instrument, and return to the original page if it has any measurement data.
Page 246: Antimony, Total - Sb-T
66. Antimony, Total - Sb-T Test Program Description：SP-800 Antimony, Total Method (0.01–0.10 ppm Sb) (PADAP Method) Instruments and Reagents: 1. SP-800 Portable Water Analyzer 2. 10-ml Sample Vial 3. 100-ml Graduated Cylinder 4. Hot Plate 5. Separatory Funnel 6. Pyxis Sb-T Reagent Program: 1.
Page 247 16. Use a soft cloth or lint free paper tissue to clean the sample vial. Place the prepared blank into the Pyxis SP-800 sample vial compartment and press the ZERO key to zero the instrument. Pyxis SP-800 will display the page.
Page 248: Place The Prepared Sample Into The Pyxis Sp-800 Sample Vial Compartment And Press The Read Key
6 o’clock position of the sample vial compartment or any position consistently. 4. Pyxis SP-800 automatically turns itself off after 2 minutes with no-key activity, except for during a measurement. Pressing and holding the OK key for 3 seconds will wake up the instrument, and return to the original page if it has any measurement data.
Page 249: Sulfide - S2
67. Sulfide - S2- Test Program Description：SP-800 Sulfide Method (0.01-0.70 ppm S ) (Methylene Blue Method) Instruments and Reagents: 1. SP-800 Portable Water Analyzer 2. 10-ml Sample Vial 3. Sulfide Reagent (Cat. No. 22445-00) Includes: Sulfide 1 Reagent (Cat. No. 1816-32) Sulfide 2 Reagent (Cat.
Page 250 EXIT key. Press the OK key to the icon menu-assisted. 11. Use a soft cloth or lint free paper tissue to clean the sample vial. 12. Repeat step 2, place the prepared blank into the Pyxis SP-800 sample vial compartment and press the ZERO key. Pyxis SP-800 Analyzer...
Page 251 6 o’clock position of the sample vial compartment or any position consistently. 4. Pyxis SP-800 automatically turns itself off after 2 minutes with no-key activity, except for during a measurement. Pressing and holding the OK key for 3 seconds will wake up the instrument, and return to the original page if it has any measurement data.
Page 252: Silica, High Range - Sihr
68. Silica, High Range - SiHR Test Program Description：SP-800 Silica High Range Method (1.0-75.0 ppm SiO2) (Silicomolybdate Method) Instruments and Reagents: 1. SP-800 Portable Water Analyzer 2. 10-ml Sample Vial 3. HACH High Range Silica Reagent Set, 10-mL sample (Cat. No. 24296-00) Includes: Acid Reagent Powder Pillows for High Range Silica (Cat.
Page 253 Note: The yellow color due to phosphate will disappear. 10. Press the TMR2 key to start the method timer, 2-minute reaction period will begin. Note: Perform Steps 9-13 within three minutes after the timer beeps Pyxis SP-800 Analyzer Procedures Manual...
Page 254 OK key to the icon menu-assisted. 12. Use a soft cloth or lint free paper tissue to clean the sample vial. 13. Repeat step 2, place the prepared blank into the Pyxis SP-800 sample vial compartment and press the ZERO key.
Page 255 Press the FORM key to select a concentration form from the list of forms (SiO2 or Si). Press the UNIT key to select a concentration unit among the list of ppb, ppm, mg/L, and ug/L. Pyxis SP-800 Analyzer Procedures Manual...
Page 256: Silica, Low Range - Silr
69. Silica, Low Range - SiLR Test Program Description：SP-800 Silica Low Range Method (0.02-1.60 ppm SiO2) (Heteropoly Blue Method) Instruments and Reagents: 1. SP-800 Portable Water Analyzer 2. 10-ml Sample Vial 3. Low Range Silica Reagent Set (Cat. No. 24593-00) Includes: Amino Acid F Reagent Powder Pillows (Cat.
Page 257 Note: Reaction time given is for samples at 20 °C (68 °F). If the sample temperature is 10 °C (50 °F), wait 2 minutes. If the sample temperature is 30 °C (86 °F), wait 30 seconds. Pyxis SP-800 Analyzer Procedures Manual...
Page 258 OK key to the icon menu-assisted. 12. Use a soft cloth or lint free paper tissue to clean the sample vial. 13. Repeat step 2, place the prepared blank into the Pyxis SP-800 sample vial compartment and press the ZERO key.
Page 259 6 o’clock position of the sample vial compartment or any position consistently. 3. Pyxis SP-800 automatically turns itself off after 2 minutes with no-Key activity, except for during a measurement. Pressing and holding the OK key for 3 seconds will wake up the instrument, and return to the original page if it has any measurement data.
Page 260: Sulfite, Low Range - So3Lr
70. Sulfite, Low Range - SO3LR Test Program Description：SP-800 Sulfite Low Range Method (0.1-5.0 ppm SO3) (OPA Method) Instruments and Reagents: 1. SP-800 Portable Water Analyzer 2. 10-ml Sample Vial 3. Pyxis SO3LR Reagent Program: 1. Press OK key (the center key) on the navigation control panel for 3 seconds until the screen lights up.
Page 261 9. Place sample vial back into the sample vial compartment and Press the TMR1 key to start the method timer, a 15-minute reaction period will begin. 10. Pyxis SP-800 will start to monitor the reaction between the reagent and the species you want to measure in the water sample.
Page 262 6 o’clock position of the sample vial compartment or any position consistently. 4. Pyxis SP-800 automatically turns itself off after 2 minutes with no-key activity, except for during a measurement. Pressing and holding the OK key for 3 seconds will wake up the instrument, and return to the original page if it has any measurement data.
Page 263: Sulfite, High Range - So3Hr
71. Sulfite, High Range - SO3HR Test Program Description：SP-800 Sulfite High Range Method (5.0-50.0 ppm SO3) (OPA Method) Instruments and Reagents: 1. SP-800 Portable Water Analyzer 2. 10-ml Sample Vial 3. Pyxis SO3HR Reagent Program: 1. Press OK key (the center key) on the navigation control panel for 3 seconds until the screen lights up.
Page 264 9. Place sample vial back into the sample vial compartment and Press the TMR1 key to start the method timer, a 15-minute reaction period will begin. 10. Pyxis SP-800 will start to monitor the reaction between the reagent and the species you want to measure in the water sample.
Page 265 6 o’clock position of the sample vial compartment or any position consistently. 4. Pyxis SP-800 automatically turns itself off after 2 minutes with no-key activity, except for during a measurement. Pressing and holding the OK key for 3 seconds will wake up the instrument, and return to the original page if it has any measurement data.
Page 266: Sulfate - So4
72. Sulfate - SO4 Test Program Description：SP-800 Sulfate Method (4.9-70.0 ppm SO4) (Turbidimetric Method) Instruments and Reagents: 1. SP-800 Portable Water Analyzer 2. 10-ml Sample Vial 3. Pyxis SO4 Reagent Program: 1. Press OK key (the center key) on the navigation control panel for 3 seconds until the screen lights up.
Page 267 7. Place sample vial back into the sample vial compartment and Press the TMR1 key to start the method timer, a 5-minute reaction period will begin. 8. Pyxis SP-800 will start to monitor the reaction between the reagent and the species you want to measure in the water sample. The concentration is shown in the chart as a function of time 9.
Page 268 6 o’clock position of the sample vial compartment or any position consistently. 4. Pyxis SP-800 automatically turns itself off after 2 minutes with no-key activity, except for during a measurement. Pressing and holding the OK key for 3 seconds will wake up the instrument, and return to the original page if it has any measurement data.
Page 269: Urea (Reactor Digestion Method) - Urea
73. Urea (Reactor Digestion Method) - Urea Test Program Description：SP-800 Urea Method (0.5-5.0 ppm) (Antipyrine Method) Instruments and Reagents: SP-800 Portable Water Analyzer Pyxis RD-800 Reactor COD/TNT adapter Pyxis Urea Reagent Program: Turn on the RD-800 Reactor. Preheat to 105 °C.
Page 270 Note: Do not move the vial from side to side as this can cause errors. Tightly cover the vial with the instrument cap. Press the ZERO key to zero the instrument. Pyxis SP-800 will display the page. Figure 386 Place the prepared sample into the Pyxis SP-800 sample vial compartment and press the READ key.
Page 271 6 o’clock position of the sample vial compartment or any position consistently. Pyxis SP-800 automatically turns itself off after 2 minutes with no-key activity, except for during a measurement. Pressing and holding the OK key for 3 seconds will wake up the instrument, and return to the original page if it has any measurement data.
Page 272: Zinc - Znxo
74. Zinc - ZnXO Test Program Description：SP-800 Zinc Method (0.2-3.0 ppm Zn) (ZnXO Method) Instruments and Reagents: SP-800 Portable Water Analyzer 10-ml Sample Vial 25-ml sample Vial Pyxis ZnXO Reagent Program: Press OK key (the center Key) on the navigation control panel for 3 seconds until the screen lights up.
Page 273 Note: There is 5 ml remaining solution in the 25-ml sample vial. Press the ZERO Key. Pyxis SP-800 will display the page. Figure 391 Press the TMR1 key to start the method timer, a 5-minute reaction period will begin.
Page 274 11. Use a soft cloth or lint free paper tissue to clean the sample vial. 12. Repeat step 2, place the prepared blank into the Pyxis SP-800 sample vial compartment and press the ZERO Key. 13. Place the prepared sample into the Pyxis SP-800 sample vial compartment and press the READ Key.
Page 275: Zinc - Zn
75. Zinc - Zn Test Program Description：SP-800 Zinc Method (0.02-3.00 ppm Zn) (Zincin Method) Instruments and Reagents: 1. SP-800 Portable Water Analyzer 2. 10-ml Sample Vial 3. Pyxis Zn Reagent 4. HACH Zinc Reagent Set (Cat. No. 24293-00) Includes: Cyclohexanone (Cat. No. 14033-32) ZincoVer 5 Reagent Powder Pillows (Cat.
Page 276 25-ml vial into another 10-ml sample vial (the prepared sample). Note: The sample will be red-orange, brown or blue, depending on the zinc concentration. 9. Press the ZERO key. Pyxis SP-800 will display the page. Figure 397 Pyxis SP-800 Analyzer...
Page 277 OK key to the icon menu-assisted. 12. Use a soft cloth or lint free paper tissue to clean the sample vial. 13. Repeat step 2, place the prepared blank into the Pyxis SP-800 sample vial compartment and press the ZERO key.
Page 278 5. Press the CONF key in the method result page to launch the method setup and calibration page. Press the UNIT key to select a concentration unit among the list of ppb, ppm, mg/L, and ug/L. Pyxis SP-800 Analyzer Procedures Manual...

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