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Laboratory Equipment
EL800
Operator's manual
Biotek EL800 Operator's Manual
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Contents
Table of Contents
Troubleshooting
Bookmarks
Table of Contents
All Rights Reserved
Restrictions and Liabilities
Trademarks
Table of Contents
Contact Information
Customer Service and Sales
European Coordination Center
Service/Tac
Document Conventions
Revision History
Intended Use Statement
Quality Control
Repackaging and Shipping
Hazards
Hazards and Precautions
Warnings
Precautions
Directive 2002/96/EC: Waste Electrical and Electronic Equipment
Directive 73/23/EEC Low Voltage
Directive 89/336/Eec:electromagnetic Compatibility
Directive 98/79/EC: in Vitro Diagnostics
Electromagnetic Interference and Susceptibility
User Safety
Safety Symbols
Chapter 1: Introduction
Introducing the Elx800 Absorbance Microplate Reader
Hardware Features
Software Features
Package Contents
Optional Accessories
Specifications
Standard Model
Ultraviolet/Uv Model
Narrow Beam/Nb Model
Product Support & Service
Contacting the Technical Assistance Center
Returning Instruments for Service/Repair
Chapter 2: Installation
Unpacking and Inspecting the Elx800
Unpacking the Instrument and Its Accessories
Figure 1: Removing the Power Supply and Shelf
Figure 2: Removing the Foam End Caps
Figure 3: Removing the Top Cover Mounting Screws
Figure 4: Removing the Shipping Block
Setting up the Elx800
Operating Environment
Electrical Connections
Power-Up and System Test
Elx800 Main Menu
Configuring Global Default Options
Figure 5: Global Configuration Options Available under UTIL
Connecting a Printer to the Elx800
Figure 6: Connectors for Printer (Parallel), Computer (Serial), and Power
Supply
Setting up the Serial Port for Communications with Other Devices
Attaching the Cable
Installing Software on the Host PC
Setting Communication Parameters
Installing Additional Filters
Figure 7: Filter Wheel Assembly
Checking the Reader's Filter Table Setting
Repackaging and Shipping the Elx800
Before Repackaging the Instrument
Repackaging the Elx800 and Its Accessories
Figure 8: Securing the Carrier
Figure 9: Hooking the Shipping Straps over the Post
Figure 10: Installing the Shipping Block
Figure 11: Reinstalling the Top Cover
Figure 12: Reattaching the End Caps
Figure 13: Placing the Unit in the Box
Preparing the Shipping Container
Chapter 3: Operation
Elx800 Front Panel
Figure 14: Keyboard
Overview
Recommendations for Achieving Optimum Performance
System Startup
Main Menu
Figure 15: Options Available from the Main Menu
Define
Programming Note
Selecting an Assay to Define
Editing the Assay Name
Define (Method, Map, Formula and Curve)
Defining METHOD
Defining MAP
Defining FORMULA
Defining CURVE
Reading a Microplate
Selecting an Assay to Run
Printing Reports
Editing Standard Outliers
Printing Results
Using the Utility Options
Setting the Date and Time
Viewing/Editing the Filter Table
Specifying Data Output and Reporting Options
Selecting Read Options
Chapter 4: Instrument Qualification
Table 1: Recommended Qualification Schedule
Recommendations for Achieving Optimum Performance
Recommended Qualification Schedule
Qualification Procedures
System Test
Checksum Test
Absorbance Plate Test
Description
Requirements
Figure 18: Sample Standards Certificate for the Absorbance Test Plate
Defining the Absorbance Test Plate Parameters
Running the Absorbance Plate Test
Test Failures
Table 2: Typical Enzyme-Substrate Combinations and Stopping Solutions
Liquid Testing
Stock Solution Formulation
Liquid Test 1
Table 3: Test Tube Dilutions
Liquid Test 2
Liquid Test 3 (for "UV" Models Only)
Table 4: PBS 10X Concentrate Solution
Chapter 5: Maintenance and Decontamination
Maintenance
Routine Cleaning Procedure
Purpose
Tools and Supplies
Procedure
Filter Storage and Handling
Replacing the Bulb
Figure 20: Lamp Assembly
Decontamination Procedure
Tools and Supplies
Procedure
Chapter 6: Troubleshooting and Error Codes
Overview
System Test Description
Glossary of Terms
Error Codes
General Errors
Fatal Errors
Appendix A: Computer Control
Overview
Controlling the Reader with Gen5
Setting up Gen5
Problems
Getting Started with Gen5
Controlling the Reader with KC4
Setting up KC4
Problems
Getting Started with KC4
Controlling the Reader with Kcjunior
Setting up Kcjunior
Problems
Getting Started with Kcjunior
Appendix B: Using 384-Well Geometry
Perform a 384-Well Plate Read in Gen5
Figure 21: Reader Configuration Dialog
Figure 22: Reader Settings Dialog
Figure 23: New Experiment Dialog
Figure 24: Experiment Workspace
Figure 25: Procedure Dialog
Figure 26: Read Step Dialog
Figure 27: Plate Reading Dialog
Perform a 384-Well Plate Read in KC4
Figure 28: KC4 Main Menu, System|Readers
Figure 29: Reader Selection Dialog
Figure 30: New Data File Dialog
Figure 31: Reading Dialog
Figure 32: Plate Reading Dialog
Perform a 384-Well Plate Read in Kcjunior
Figure 33: Setup|Reader Dialog
Figure 34: Reader Setup Dialog
Figure 35: Read Plate Dialog
Figure 36: Protocol Definition Dialog
Figure 37: Example Read Method Parameters
Appendix C: Report Format
Figure 38: Samples with Calls on Matrix Report
Figure 39: Curve Fit Report
Figure 40: Samples with Calls on Column Report
Figure 41: Column Report Without Samples
Figure 42: Panel Report
Figure 43: Assay Detail Report (Sheet 1 of 2)
Figure 44: Assay Detail Report (Sheet 2 of 2)
Appendix D: Comparison of the Elx800 and the EL800
Appendix E: Instructions for Programming a New Assay
Sample ANA Screen Enzyme Immunoassay Kit
Intended Use
Background
Principle of the Assay
Materials
Quality Control and Results
Expected Values
Programming the ANA Screen Enzyme Immunoassay Kit
Sample Human Anticardiolipin Igg Enzyme Immunoassay Kit
Intended Use
Background
Principle of the Assay
Materials
Quality Control and Results
Expected Values
Programming the Human Anticardiolipin Igg Enzyme Immunoassay Kit
Programming the Human Anticardiolipin Igg
Enzyme Immunoassay Kit
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Absorbance Microplate Reader
ELx800
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Operator's Manual
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