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BÜCHI Fat Determination B-820/B-821
1
2
2.1
2.2
2.3
2.4
2.5
2.6
2.7
2.8
2.9
3
3.1
3.2
3.2.1
3.2.2
3.2.3
3.2.4
4
4.1
4.2
4.3
4.4
4.5
4.6
4.7
4.8
4.9
4.10
4.11
4.11.7 Connecting the signal cable to the B-820 unit
head of the B-820 unit
Contents
3
5
5.1
7
5.2
7
5.2.1
7
5.2.2
7
5.2.3
8
5.2.4
8
5.2.5
9
5.2.6
10
5.2.7
10
5.2.8
10
5.2.9
11
12
14
6
14
6.1
14
6.2
14
6.3
14
6.4
6.5
15
6.6
15
6.7
15
6.8
16
6.9
16
6.10
18
6.11
18
20
21
22
22
7
22
7.1
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7.2
7.3
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23
8
23
23
9
24
9.1
24
9.2
24
9.3
9.4
25
9.5
26
26
en, Version H (66 pages)
B-820/B-821 Instructions
Contents
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Order no.
96571
1
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Summary of Contents for Buchi B-820
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Page 1: Table Of Contents
4.11.5 Removing the transportation belt Appendix 4.11.6 Installing the syringe/needle assembly Technical data 4.11.7 Connecting the signal cable to the B-820 unit Error messages 4.11.8 Connecting power to the Autosampler B-821 Materials used 4.11.9 Aligning the syringe needle with the injection... - Page 2 BÜCHI Fat Determination B-820/B-821 Please read these instructions thoroughly before using the Fat Determination Unit B-820/B-821. These instructions should be kept near the unit for general reference. Section 2 contains important notes on safety, which must be observed if the Fat Determi- nation Unit is to be operated safely.
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Page 3: Scope Of Delivery
BÜCHI Fat Determination B-820/B-821 1 Scope of delivery Scope of delivery Figure 1: Complete view of B-820/B-821 Figure 2: Complete view of B-820, Manual Manual basic system incl. individual Version B-815 B-820 B-821 Total fat order no. fatty acids order no. - Page 4 1 Scope of delivery BÜCHI Fat Determination B-820/B-821 Accessories Manual Basic System Accessories B-815 (refer to Manual B-815) Accessories B-820 Manual (refer to Table 2) Accessories Automatic Basic System Accessories B-815 (refer to Manual B-815) Accessories B-820 Automatic (refer to Table 3)
- Page 5 Type Grounded (shock-proof) 10016 Type GB 17835 Type AUS 17836 Type USA, 230 V 33763 1 Instruction manual available in the following languages: German 96570 English 96571 French 96572 Italian 96573 Spanish 96574 1 Packaging 34560 Table 3: Accessories B-820 Automatic...
- Page 6 1 Scope of delivery BÜCHI Fat Determination B-820/B-821 Accessories B-821 Automatic 1 Septum 1 Cylinder (reservoir) holder 2 Glass cylinders for rinsing solvent and waste 37069 1 Carousel 37085 1 Serial number label for carousel 37103 1 Mounting plate 1 Sample vessel 2ml, compl. (100 )
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Page 7: Safety
Fat Determination Unit B-820, or automatically into the Fat Determination Unit with Autosampler B-821. The detection occurs with a flame ionization detector (FID). The subsequent evaluation is made by integrating the total peak areas. -
Page 8: Improper Use
2 Safety BÜCHI Fat Determination B-820/B-821 Improper use Any use other than those already listed, and any application that does not conform with the technical data is deemed to be improper use. The customer shall bear sole responsibility for any damage resulting from improper use. -
Page 9: Safety Measures
The Instruction Manual is a component of the Fat Determi- nation Unit B-820/B-821 and should remain with the unit for easy reference by any user. This also applies to the additional language variants of these instructions, which can be ordered... -
Page 10: Handling Of Gas Cylinders In The Laboratory
2 Safety BÜCHI Fat Determination B-820/B-821 Handling of gas cylinders in the laboratory Gas cylinders should be handled according to the pertinent local regulations and provisions. The following are general safety measures. 1. Protect gas cylinders from excessive heat, mechanical damage and corrosive substances. -
Page 11: Function
BÜCHI Fat Determination B-820/B-821 3 Function Function The Fat Determination Unit B-820, which can be completed with the Autosampler B-821 (refer to Figure 5) is an instrument which determines total fat on the basis of fatty acids or individual fatty acids in organic matrices using a gas-chromatographic technique. -
Page 12: Operational Principles
BÜCHI Fat Determination B-820/B-821 3 Function Operational principles Figure 6: Diagram Synthetic air Hydrogen (percentage purity 5.0) Pressure reducing valve for synthetic air Pressure reducing valve for hydrogen Electronic valve for synthetic air Electronic valve for hydrogen Damping tube for synthetic air... - Page 13 BÜCHI Fat Determination B-820/B-821 3 Function Depending on the determination parameters, nine different determination modes can be selected: Mode Abbreviat. Description Factor General Fact.gen Determines the factor that will be necessary for the determination of the total fat content. General correction Gen.corr.
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Page 14: System Description Autosampler
It is mounted on the Büchi Fat Determination Unit B-820 above the injection head. The Autosampler is operated through the keypad of the B-820. The Autosampler is powered through the B-820 via a connection cable. -
Page 15: Putting Into Operation
BÜCHI Fat Determination B-820/B-821 4 Putting into operation Putting into operation Unpacking and checking the B-820 unit Open the carton carefully in order to avoid breaking the glass of the enclosed separation column, soap bubble meter and syringe. Unpack the unit with care and check for any possible damage. -
Page 16: Power Connections
All analytical balances of the type MC1 as well as balances with interface MP8-4. A&D: FR and HA series. The interface parameters in B-820 are preset and cannot be modified. The balance parameters are as follows: • 2400 baud • even parity •... - Page 17 The printer connection cable is available as accessories (cable length: 3 meters, type: Centronics): B-820 Printer cable 30754 The B-820 supports the IBM character set. Please consult printer operating instructions before making adjustments! Autosampler B-821 Installation of the Autosampler is described in Section 4.11.
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Page 18: Filling The Formic Acid
0 to 10 bar (0 to 150 psi). The output pressures at the valves to the B-820 unit should be 5.0 bar (72.5 psi) both for synthetic air and for hydrogen. - Page 19 (labelled with “H ” and “Synth. Air”) on the back of the B-820. (See Figure 10: Gas connection layout, Page 19) Ensure the correct connection of the gas cylinder to the unit.
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Page 20: Checking Supply Lines For Leaks
4 Putting into operation BÜCHI Fat Determination B-820/B-821 Checking supply lines for leaks After connecting the pressure lines to the unit, open both cyl- inder valves before switching on the unit, and then close them again once the pressure is achieved. The pressure indicator on the pressure reducing valve should remain constant for approx. -
Page 21: Installing The Separation Column
BÜCHI Fat Determination B-820/B-821 4 Putting into operation Installing the separation column 1. Open the oven door 2. Position the seals, screws and nuts as shown in Figure 11. 3. Figure 12 shows how to fit the separation column. Push both sides of the column up as far as they will go, hold in place and tighten nuts by hand. -
Page 22: Setting The Correct Gas Flow Rate
4 Putting into operation BÜCHI Fat Determination B-820/B-821 Setting the correct gas flow rate The gas pressures are electronically controlled, digitally displayed and preset to a basic value by the manufacturer. However, this basic value does not ensure the correct gas flow rate and must be adjusted after preheating. -
Page 23: Retrofitting A Manual Basic System With An Autosampler
The screws go through the holes of the mount- ing plate and reach the baseplate. 4.11.4 Mounting the Autosampler on the B-820 unit In order to mount the Autosampler on the B-820 unit proceed as follows: 1. Place the Autosampler on the mounting plate just mounted . -
Page 24: Installing The Syringe/Needle Assembly
A data cable is fitted to the Autosampler B-821 which emerges at the rear of the unit. Insert the cable at the rear of the B-820 unit into the corresponding interface (refer to Figure 8: Connection of periphery units at the rear of the unit). -
Page 25: Aligning The Syringe Needle With The Injection
5. Connect the power cable to the rear of the Autosampler and to the B-820 unit (refer to Figure 8: "Connection of Figure 15: Power entry module periphery units at the rear of the unit“) •... -
Page 26: Connecting The Pneumatics To The Autosampler
4.11.11 Installing the solvent reservoir bracket The bracket holds two solvent bottles of 180 ml each and is secured on the lower left side of the B-820 unit by means of an M5*12 screw. For a more secure fix we recommend to... - Page 27 1. All PTFE lines which emerge from the bottom of the Autosampler are pushed between the cover and the housing of the B-820 unit and are secured with a cable binder on the pre-assembled binder base to the rear panel of the B-820 unit.
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Page 28: Operation
5 Operation BÜCHI Fat Determination B-820/B-821 Operation Ensure that the unit has been commissioned according to the instructions in Section 4, Putting into operation. Control, operating and display elements The main power switch is located at the back of the unit. - Page 29 BÜCHI Fat Determination B-820/B-821 5 Operation Moves the cursor down within a menu. Confirms position of cursor and performs menu command. Allows selection of a variety of options (e.g. balances, print system, frequency etc.). In the sample weight table, pressing the OPTION key copies a field into the next row.
- Page 30 5 Operation BÜCHI Fat Determination B-820/B-821 Use the keyboard to enter sample identification and report titles and press this key to cancel. The key has the same function as this key, "ENTER".
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Page 31: Operating The Unit
BÜCHI Fat Determination B-820/B-821 5 Operation Operating the unit The following flow chart shows the operating structure. This is followed by a systematic description of all the options provided by this unit. The unit contains a help program which can be accessed by... - Page 32 5 Operation BÜCHI Fat Determination B-820/B-821 Version: 3.00af Gas supply on? User: C.Heiter Press <ENTER> to cont. Preheating p H2 55kPa Sample weight/Mode p air 50kPa Determination T oven 130°C Data manager T inj. 220°C Setup T FID 260°C Determination "Total fat"...
- Page 33 BÜCHI Fat Determination B-820/B-821 5 Operation Gas flow setup Data output p H2 55kPa User configuration Statistic p air 50kPa Printer configuration Clear data T oven 130°C Main menu T inj. 220°C System configuration T FID 260°C Autosampler Main menu "Gas flow setup for H2"...
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Page 34: User Configuration
5 Operation BÜCHI Fat Determination B-820/B-821 5.2.1 User configuration Position cursor on <Setup>. Preheating p H2 55kPa Sample weight/Mode p air 50kPa Determination T oven 130°C Data manager T inj. 220°C Setup T FID 260°C Position cursor on <User configuration> command. -
Page 35: Printer Configuration
BÜCHI Fat Determination B-820/B-821 5 Operation 5.2.2 Printer configuration Position cursor on <Setup> . Preheating p H2 55kPa Sample weight/Mode p air 50kPa Determination T oven 130°C Data manager T inj. 220°C Setup T FID 260°C Position cursor on <Printer configuration>. -
Page 36: System Configuration
5 Operation BÜCHI Fat Determination B-820/B-821 5.2.3 System configuration Position the cursor on <Setup>. Preheating p H2 55kPa Sample weight/Mode p air 50kPa Determination T oven 130°C Data manager T inj. 220°C Setup T FID 260°C The cursor is positioned on <Gas flow setup>. Position the Gas flow setup cursor on <System configuration>. -
Page 37: Autosampler Configuration
BÜCHI Fat Determination B-820/B-821 5 Operation PC-Interface This menu is meant for the setup of interface parameters for Baudrate: 9600 the PC interface. The cursor is positioned on Baudrate.The Data Bits: Baudrate cannot be changed. Stop Bits: Position cursor on Data bits. Press the OPTION key to select... -
Page 38: Preheating/Separation Column Conditioning
5 Operation BÜCHI Fat Determination B-820/B-821 5.2.5 Preheating/Separation column conditioning If the unit has been turned off for longer than 30 minutes, the unit must be preheated for 30 minutes before a determination or gas flow setup procedure is performed. -
Page 39: Gas Flow Rate Setup
BÜCHI Fat Determination B-820/B-821 5 Operation Position the cursor on <Start preheating>. Time: 2400s p H2 55kPa Start preheating p air 50kPa Main menu T oven 130°C T inj. 220°C T FID 260°C The preheating procedure is in operation (running LED is lit up). - Page 40 5 Operation BÜCHI Fat Determination B-820/B-821 1. In order to check the hydrogen flow rate, the cursor is positioned on <Press. system: H2> and the air pressure is switched off internally (p air: 0 kPa). Connect the soap bubble meter, containing approx. 2 ml soap solution, to the FID (see figure 19) and wait until the Ready LED lights 2.
- Page 41 BÜCHI Fat Determination B-820/B-821 5 Operation 5. To check the air flow rate, the cursor is positioned on Determination "Total Fat" (short column) <Press. system: Air> during which the air pressure is built Press. system: air p H2 55kPa up. Wait until the actual pressure value is identical to the...
- Page 42 5 Operation BÜCHI Fat Determination B-820/B-821 Re-check the gas flow rate and the supply lines for leakages (see Section 4.7, Checking supply lines for leaks) each time the gas cylinder or column is changed as well as after service interventions.
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Page 43: Sample Weight And Mode Adjustment
Setup T FID 260°C Manual sample weight If no balance is connected to the B-820, the sample weights must be entered manually in the sample weight table using the keypad. Confirm “Manual sample weight” with “ENTER”. The sample weight table appears. - Page 44 5 Operation BÜCHI Fat Determination B-820/B-821 After the sample identification (Name) has been entered and been confirmed with “ENTER”, the cursor automatically moves to the next free cell. In doing this, the mode of the previous sample will also be transferred, and must be changed - where required - using the “OPTION”...
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Page 45: Determination
BÜCHI Fat Determination B-820/B-821 5 Operation Factors table Factor Fat: 0.953 Factor butyric acid: 1.023 Fat factor 28.570 Entry of the factor that will be used for the determination in Blank value but. acid 0.0031 g the “gen.corr.” and “spec.corr.” modes. - Page 46 5 Operation BÜCHI Fat Determination B-820/B-821 Determination with the Autosampler Sample No. Sample no: p H2 55 kPa Enter the sample number (from the sample weight table) with Vial no: p air 50 kPa which the determination should be started. Confirm it with...
- Page 47 BÜCHI Fat Determination B-820/B-821 5 Operation Procedure The sample will be injected automatically. The corresponding temperature program runs automatically in the background according to the determination mode (see Chapter 3, Table 7). At the end of the determination, the venting flap opens;...
- Page 48 5 Operation BÜCHI Fat Determination B-820/B-821 Determination with manual syringe Sample No. Sample no: p H2 5 5 k P a Entry of the sample number (from the sample weight table) Next number p air 5 0 k P a that should be determined as the next sample.
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Page 49: Data Output
BÜCHI Fat Determination B-820/B-821 5 Operation 5.2.9 Data output Preheating p H2 55kPa Sample weight/Mode p air 50kPa Set the cursor on “Data manager” and confirm with “ENTER”. Determination T oven 130°C Data manager T inj. 220°C Setup T FID 260°C... - Page 50 5 Operation BÜCHI Fat Determination B-820/B-821 Place the cursor on “Main Menu” and confirm with “ENTER”. Device: Printer int. First sample: What will be printed out? Last sample: Start output Data output “Display” Main menu For all modes, only the data of the “first sample” (inc. all injec- tions) will be shown in the display.
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Page 51: Statistics
BÜCHI Fat Determination B-820/B-821 5 Operation 5.2.10 Statistics Sample statistics Up to 9 injections can be carried out with the same sample. The following characteristics are obtained from these (x ) values Content value x • Average sample value: Content value x •... - Page 52 5 Operation BÜCHI Fat Determination B-820/B-821 Set the cursor on “Data manager” and confirm with “ENTER”. Preheating p H2 55kPa Sample weight/Mode p air 50kPa Determination T oven 130°C Data manager T inj. 220°C Setup T FID 260°C Set the cursor on “Statistic” and confirm with “ENTER”.
- Page 53 BÜCHI Fat Determination B-820/B-821 5 Operation Elimination N a m e R e s u l t E l i m The elimination of individual measurements. Place the cursor 5 . 1 Cereal 1 7.23g/100g y e s on the “Eliminate” row and confirm with “ENTER”. A table of 5 .
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Page 54: Clearing Data
5 Operation BÜCHI Fat Determination B-820/B-821 5.2.11 Clearing data Preheating p H2 55kPa Sample weight/Mode p air 50kPa Place cursor on “Data manager” and confirm with “ENTER”. Determination T oven 130°C Data manager T inj. 220°C Setup T FID 260°C Place the cursor on “Clear data”... -
Page 55: Maintenance
BÜCHI Fat Determination B-820/B-821 6 Maintenance Maintenance Control checks All regulations designed to ensure that the Fat Determination Unit is kept in proper working order must be complied with. These include periodic cleaning and inspections to check for possible damage. -
Page 56: Cleaning The Fid
6 Maintenance BÜCHI Fat Determination B-820/B-821 Cleaning the FID 1. Switch off unit Allow unit to cool down 2. Disconnect detector cable (= signal cable 3. Disconnect ignition cable 4. Loosen main arm of FID's top body 5. Remove FID's top body 6. -
Page 57: Checking The Formic Acid
Caution: the container is under pressure • Do not open if the B-820 is warm or is in operation • Do not refill if the B-820 is warm or is in operation • Do not overfill, max. 20 ml or at less 1 cm below the vessel's arms! •... -
Page 58: Changing The Ribbon
6 Maintenance BÜCHI Fat Determination B-820/B-821 Changing the ribbon Press lightly on the left hand side of the ribbon (<Push>) - this enables the ribbon to be lifted out. Feed the paper into the slit of the new ribbon, insert the new ribbon and press down lightly. -
Page 59: Maintenance Of The Autosampler
BÜCHI Fat Determination B-820/B-821 6 Maintenance 6.11 Maintenance of the Autosampler 6.11.1 General Optimum performance of the Autosampler is ensured by rou- tine and preventive maintenance procedures. The procedures described below should reflect the frequency and duration with which the Autosampler is used. -
Page 60: Taking Out Of Operation
B-820 unit and farmic acid vessel emptied before moving the unit. Disposal To ensure that disposal of the B-820 and B-821 units is as environmentally friendly as possible, a list of the materials used, some of which are marked with material codes, is provided in Section 9.3, "Materials used". -
Page 61: Replacement Parts
Inspection by or assignment to an unofficial third party is prohibited. The reproduction or manufacture of any parts found in this manual is also prohibited. Replacement parts detection unit B-820 Order no. Paper roll 26341 Printer ribbon... -
Page 62: Appendix
Power input* max. 2 kW Current consumption max. 9A Ambient temperature 5 – 40°C Table 10: Technical data B-820 B-821 Unit dimensions, 155 x 495 x 400 mm (width x height x depth) Packaging dimensions, 630 x 460 x 680 mm... -
Page 63: Error Messages
BÜCHI Fat Determination B-820/B-821 9 Appendix Error messages The unit is equipped with a comprehensive error message system, which will inform the user of the steps that can be taken when a system error occurs or following an incorrect application. -
Page 64: Materials Used
9 Appendix BÜCHI Fat Determination B-820/B-821 If the measuring results continue to deteriorate, check the following possible sources of error: 1. Extraction procedure insufficient (e.g. time) 2. Poor gas quality 3. Sufficient formic acid in the bottle 4. Separation column possibly defective or worn 5. -
Page 65: Fcc Requirements (For Usa And Canada)
BÜCHI Fat Determination B-820/B-821 9 Appendix FCC requirements (for USA and Canada) English: This equipment has been tested and found to comply with the limits for a Class A digital device, pursuant to both Part 15 of the FCC Rules and the radio interference regulations of the Canadian Department of Communications. These limits are designed to provide reasonable protection against harmful interference when the equipment is operated in a commercial environment. -
Page 66: Declaration Of Conformity
9 Appendix BÜCHI Fat Determination B-820/B-821 Declaration of conformity BÜCHI Labortechnik AG Postfach, CH-9230 Flawil Switzerland declare under our sole responsibility that the product: BÜCHI Fat Determination B-820/B-821 to which this declaration relates is in conformity with the following standards:...
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