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Hach Eclox User Manual

Hach Eclox User Manual

Rapid response test kit
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2886888
™
Eclox
Rapid Response Test Kit
User Manual
01/2018, Edition 5

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  • Page 1 2886888 ™ Eclox Rapid Response Test Kit User Manual 01/2018, Edition 5...
  • Page 2 Visit us at www.hach.com...

  • Page 3: Table Of Contents

    Table of Contents Section 1 Specifications..................7 Section 2 General information................9 2.1 Safety information ....................9 2.1.1 Use of hazard information................. 9 2.2 Product overview....................10 2.2.1 Test descriptions ..................10 2.2.2 Test setup ....................12 2.3 Unpack the instrument ..................13 Section 3 Luminometer ..................
  • Page 4 Table of Contents Section 6 Pesticide/Nerve Agent Test..............39 6.1 Pesticide/Nerve Agent procedure ..............39 Section 7 Free and Total Chlorine Test..............41 7.1 Test preparation ....................41 7.2 Free or total chlorine procedure, 0–3.4 mg/L Cl2 ..........41 Section 8 Color Test ....................43 8.1 Test preparation ....................43 8.2 Color, apparent (0–100 APHA platinum cobalt color units) .......43 8.3 Color, apparent (0–500 APHA platinum cobalt color units) .......45 Section 9 Pocket Pro™+ Multi 2 Tester ...............47...
  • Page 5 Table of Contents 11.4 Reagent description ..................74 11.5 Reagent storage and preservation ..............75 11.6 Prepare the reagent ..................75 11.6.1 Prepare the stock suspension using the LCK491 reagent ....75 11.6.2 Prepare the test suspension ..............77 11.7 Sample collection, storage and preservation ..........83 11.8 Interferences ....................
  • Page 6 Table of Contents Table of Contents Page 6 EcloxTOC.fm...

  • Page 7: Section 1 Specifications

    Section 1 Specifications Specifications are subject to change without notice. General Dimensions 520 x 450 x 215 mm (20.5 x 17.5 x 8.5 in.) Weight 9 kg (20 lb), fully loaded Temperature Tested from –20 to 55 °C (–4 to 131 °F) Chemiluminescence Toxicity Testing Modes Luminescent Bacteria Toxicity Testing...
  • Page 8 Chlorine (free) Range 0 to 3.4 mg/L Chlorine (total) Range 0 to 3.4 mg/L Color Range 0 to 100, 0 to 500 APHA Platinum-Cobalt Color units Pocket Pro™+ Multi 2 Tester pH: 0.0 to 14.0 pH TDS: Auto-ranging (0.0 to 99.9 ppm, 100 to 999 ppm, 1.00 to Range 10.00 ppt) Accuracy...

  • Page 9: Section 2 General Information

    Section 2 General information In no event will the manufacturer be liable for direct, indirect, special, incidental or consequential damages resulting from any defect or omission in this manual. The manufacturer reserves the right to make changes in this manual and the products it describes at any time, without notice or obligation.

  • Page 10: Product Overview

    This symbol indicates the need for protective eye wear. 2.2 Product overview The Eclox™ Rapid Response Test Kit is used to do first line water testing. The kit is a generic qualitative test that gives a broad indication of water quality. To do a proper toxicity test, identify a baseline using the product in the waters to be tested.
  • Page 11 reduces the amount of light that is created. The more pollutant that is in the sample, the less light that is created. The light made by the sample water is compared to a pure water reference and the percentage inhibition of the light made is measured and made known.

  • Page 12: Test Setup

    • Luminometer test—test the operation of the luminometer before Chemiluminescence Toxicity Tests or Luminescent Bacteria Toxicity Tests are done. • Luminometer calibration—calibrate the luminometer each day before Chemiluminescence Toxicity Tests are done. • Measure samples—measure the samples with the tests. Visit us at www.hach.com...

  • Page 13: Unpack The Instrument

    2.3 Unpack the instrument Remove the Eclox Rapid Response Test Kit from the shipping carton and check it for any visible damage. If any items are missing or damaged, contact the manufacturer or a sales representative immediately. • Carrying case •...

  • Page 15: Section 3 Luminometer

    Luminescent Bacteria Toxicity Test to measure and record relative light units made by the reagents when exposed to samples. The Eclox luminometer is made for use under extreme field conditions. The Eclox luminometer components are rugged, easy to use and reliable (refer to...

  • Page 16: Prepare The Luminometer For Use

    Figure 2 Function keys Item Description Function number Soft key Does the action on the display directly above the key. Soft key Does the action on the display directly above the key. Back light button Illuminates the display. Off button Removes power to the instrument.

  • Page 17: Erase The Results Saved On The Luminometer

    14. Push and hold QUIT for a few seconds. The Systems Test Menu screen is shown. 15. Select Return to the ECLOX Main Menu (or LBT Main Menu) and push ENTER. 3.2.2 Erase the results saved on the luminometer Push ON (green button) for several seconds to apply power to the instrument.

  • Page 18: Set The Measurement Range

    (e.g., 70–100% light inhibition). 3.2.3.1 Eclox chemiluminescence test To show or change the measurement range for the Eclox chemiluminescence test: Push ON (green button) to apply power to the instrument. The instrument does built-in tests that make sure that the electronics and software are operating correctly.
  • Page 19 The Set-up Menu is shown. Select Set Measurement Range and push ENTER. The current range is shown. Push YES to confirm. To change the range, push CHANGE Push STORE to save the change. The Set-up Menu is shown. 3.2.3.2 Luminescent Bacteria Test If the measurement range is set to the 0–2000 light units and the measuring value is marked with an * (e.g., 2010*) or the lumiometer shows Detector Overload, the measurement is above the set measurement range.

  • Page 20: Change The Default Settings

    • Measuring time—the amount of time the sample is measured by the luminometer. Note: There is no need to change the default settings of 8 seconds waiting time or 7 seconds measuring time unless HACH or HACH-LANGE customer service asks the user to do so.

  • Page 21: Connect The Luminometer To A Printer

    To show or change the waiting time and measuring time: Push ON (green button) to apply power to the instrument. The instrument does built-in tests that make sure that the electronics and software are operating correctly. When the built-in tests are done, push PROCEED. The Main Menu is shown.
  • Page 22 Put an adapter (DB9, 3 wires, male-male, 2-3, 3-2, 5-5. Cross over, not straight) on the other end of the RS232 serial interface cable. Connect the RS232 serial interface cable to the printer. If using a DPU-414 thermal printer: Configure the printer (refer to the printer manual for more information): DIP switch Switch Position...

  • Page 23: Connect The Luminometer To A Computer

    When Write? is shown, push PAPER FEED SW. Turn on the printer. If not using a DPU-414 thermal printer, configure the printer: Option Setting Data length 8 bits Parity setting Parity condition Busy control XON/XOFF Baud rate 9600 bps 3.5 Connect the luminometer to a computer To connect the luminometer to a computer: Install the LUMISsoft software on the computer (refer to Install LUMISsoft on...

  • Page 25: Section 4 Chemiluminescence Toxicity Test

    Section 4 Chemiluminescence Toxicity Test The Chemiluminescence Toxicity Test uses the luminometer. Before doing the Chemiluminescence Toxicity Test, read section 3.1, Overview on page 15 and do the procedures in section 3.2, Prepare the luminometer for use on page 4.1 Overview The Chemiluminescence Toxicity Test and Luminescent Bacteria Toxicity Test both show the inhibitory effects of a sample.

  • Page 26: Prepare Ct Reagent 2

    4.2.1 Prepare CT Reagent 2 1. Remove the CT 2. Carefully put all of the 3. Put the caps back on Reagent 2 buffer and CT CT Reagent 2 buffer into the bottles. Shake the CT Reagent 2 caps. the CT Reagent 2 bottle. Reagent 2 bottle for 30 seconds.
  • Page 27 4. Put the tip in the CT 5. Put the tip into CT 6. Remove the tip from Reagent 3 concentrate Reagent 3 and dispense the pipet and put in the 1 cm below the surface. the liquid by gently pushing waste bag.

  • Page 28: Calibrate The Luminometer

    When the built-in tests are done, push PROCEED. The Main Menu is shown. Select ECLOX and push ENTER. The ECLOX Main Menu is shown. Push ENTER to select Measure. The Measure Menu is shown. Select Measure Reference and push ENTER.
  • Page 29 16. Put the pipette tip into the cuvette and remove the pipette from the cuvette to remove any drops from the outside of the tip. 17. Remove the lids from the CT Reagents 1, 2 and 3. 18. Put a yellow pipette tip on to the yellow pipette. 19.

  • Page 30: Measure Pollutants In The Water Sample

    The Main Menu is shown. Note: Two drops of pre-conditioner reagent can neutralize up to 15 mg/L of chlorine. 4. Select ECLOX and push 5. Open the luminometer 6. Push PROCEED to ENTER. lid and make sure that a show the test status.
  • Page 31 7. Put one cuvette from the 8. Put a blue pipette tip on 9. Push in the operating Cuvettes and 1000 µL the blue pipet. button on the pipet to the Pipet Tip Set into the black stop. cuvette holder. 10.
  • Page 32 16. Close the lid. Push 17. The Inhib% is shown 18. Push PROCEED to go PROCEED. on the screen. Record the back to the Measure Menu. Inhib% value and graph on The luminometer the Test Record Sheet. automatically starts measuring. After four Note: For sea water samples, minutes, the screen timer the graph may appear higher...

  • Page 33: Show The Previous Results

    The luminometer does built-in tests that make sure that the electronics and software are operating correctly. When the built-in tests are done, push PROCEED. The Main Menu is shown. Select ECLOX and push ENTER. Select Previous Results and push ENTER. The Previous Results Menu is shown. To show previous results: Select Recall Results and push ENTER.
  • Page 34 When the built-in tests are done, push PROCEED. The Main Menu is shown. Select ECLOX and push ENTER. Select Previous Results and push ENTER. The Previous Results Menu is shown. Select Download to PC and push ENTER.

  • Page 35: Section 5 Ez Arsenic Test Kit

    Section 5 EZ Arsenic Test Kit DANGER: Hydrogen and arsine gasses are generated during the test. Work in a well-ventilated area away from open flames and other sources of ignition. Review Material Safety Data Sheets for safe handling, storage and disposal information. 5.1 Test preparation •...
  • Page 36 4. Immediately attach the 5. Remove the test strip cap to the reaction bottle. and immediately compare Swirl continuously for 60 the developed color to the seconds. chart on the test strip bottle Do not shake or invert or (0–500 ppb row). allow sample to get on Read strips in the shade.

  • Page 37: Ez Arsenic, 0-4000 Ppb (0, 35, 75, 175, 1500, 4000)

    5.3 EZ Arsenic, 0–4000 ppb (0, 35, 75, 175, 1500, 4000) 1. Insert a test strip into the 2. Fill the square 3. Add one Reagent #1 cap so the pad completely measuring vial to the top and one Reagent #2 covers the small opening.

  • Page 38: Optional Procedure For Removing Sulfide

    Insert the test strip as detailed in step 1 of the 0–500 or 0–4000 ppb test procedure and continue with the test. Note: The lead acetate must not touch the test strip! Always wear gloves or wash hands thoroughly after handling lead acetate. Visit us at www.hach.com...

  • Page 39: Section 6 Pesticide/Nerve Agent Test

    Section 6 Pesticide/Nerve Agent Test 6.1 Pesticide/Nerve Agent procedure 1. Remove one pesticide 2. The pesticide strip has a 3. Put the white strip from the storage case. white disc at one end and a disc in the beaker that Open the foil packet on the larger pink disc at the other contains the sample water...
  • Page 40 7. Open the strip and look 8. There are two possible 9. Do the test again if a at the color of the smaller results: positive result is seen or disc. For the best results, compare with a test from hold the strip against A white disc indicates a known clean water...

  • Page 41: Section 7 Free And Total Chlorine Test

    Section 7 Free and Total Chlorine Test 7.1 Test preparation Important Note: This product has not been evaluated to test for chlorine and chloramines in medical applications in the United States. • Analyze samples immediately after collection. • Put the color disc on the center pin in the color comparator box (numbers to the front).
  • Page 42 4. Add one DPD (Free or 5. Swirl to mix. A pink color 6. Put the second tube Total) Chlorine Powder develops. into the color comparator Pillow to the second tube. For free chlorine, read the box. result within 1 minute. For total chlorine, wait 3 minutes.

  • Page 43: Section 8 Color Test

    Section 8 Color Test 8.1 Test preparation • Put the color disc on the center pin in the color comparator box (numbers to the front). • Use sunlight or a lamp as a light source to find the color match with the color comparator box.
  • Page 44 4. Fill a second tube to the 5. Hold the color 6. Read the results in top line with sample. comparator box below a platinum cobalt color units Put the second tube into light source. Turn the color in the scale window. the color comparator box.

  • Page 45: Color, Apparent (0-500 Apha Platinum Cobalt Color Units)

    Color, apparent (0–500 APHA platinum cobalt color units) 1. If installed, remove the 2. Fill a tube to the first line 3. Put the tube into the longpath adapter. (5 mL) with deionized water left opening of the color or water that has no color. comparator box.

  • Page 47: Section 9 Pocket Pro™+ Multi 2 Tester

    Section 9 Pocket Pro™+ Multi 2 Tester 9.1 pH or TDS measurement 1. Push the 2. Rinse the sensor and the 3. Push and hold the Power/Backlight key to set sensor cap with deionized Lock/Parameter key to the power to on. Remove water.
  • Page 48 7. Push the 8. Rinse the sensor and the 9. For faster response Power/Backlight key to set sensor cap with deionized and longer test life, put the power to off. water. Dry with a no-lint several drops of cloth. deionized water in the sensor cap.

  • Page 49: Ph Calibration

    9.2 pH calibration 1. Push the 2. Rinse the sensor and the 3. Push and hold the Power/Backlight key to set sensor cap with deionized Lock/Parameter key until the power to on. Remove water. Dry with a no-lint “pH” shows as the the sensor cap.

  • Page 50: Tds/Conductivity Calibration

    7. Wait for the value to 8. Optional: Do steps 2 9. Push and hold the stabilize, then push the through 7 againto measure Calibration/Settings key Calibration/Settings key to a pH 4.00 and/or a pH to exit. save the buffer value. 10.00 buffer.
  • Page 51 4. Pour a 1413 µS/cm 5. Put the sensor fully into 6. Push the conductivity standard to the sensor cap. The tester Calibration/Settings key the fill line on the sensor will read the standard to start the calibration. cap. value. Air bubbles under the probe tip when submerged can cause slow...

  • Page 53: Section 10 Luminescent Bacteria Toxicity Test: Screening/Limit Measure

    3.2, Prepare the luminometer for use on page • Print color copies of the Screening Luminescence Results Sheet to use in the field (refer to page 68) from www.Hach.com. This chapter describes the LBT screening luminescence procedure and LIMIT measure procedure and contains the procedure steps.

  • Page 54: Accuracy

    Accordingly, a test must be done for each batch of bacteria that is prepared in-house or moved in. The quality certificate delivered with each package of luminescent bacteria reagent by HACH-LANGE GmbH guarantees compliance with the stipulated validity criteria. To make sure that the test operates correctly on site, the user does control measurements with the standard solutions (refer to the ISO standard procedure).

  • Page 55: Reagent Storage And Preservation

    sources of supply is contained in the quality certificate that comes with every box of luminescent bacteria reagent. The standard stock solutions should be prepared with 2% NaCl solution. The pH of the sample should not be adjusted. Prepare the standard solution such that 0.5 mL of standard solution and 0.5 mL of bacteria solution gives the above mentioned final test concentration.
  • Page 56 The stock suspension can be kept in a refrigerator as long as the validity criteria are met (typically up to 4 hours). This procedure is temperature sensitive. 1. Remove the 2. Put the frozen 3. In the field, remove the luminescent bacteria test luminescent bacteria cap from the reconstitution...
  • Page 57 7. Put the tip of the pipette 8. Put the tip of the pipette 9. Put the rubber stopper in into the reconstitution into the luminescent the reagent bottle. Swirl the solution and slowly pull in bacteria reagent bottle and reagent bottle to mix.

  • Page 58: Prepare The Test Suspension

    10.7 Prepare the test suspension Prepare the test suspension (stock suspension and Diluent mixture) by doing the procedure in this section. The Diluent is made according to ISO11348-3 and makes sure that the test is not negatively affected by the presence of potassium (K+) and magnesia (Mg2+) ions in the sample.

  • Page 59: Sample Collection, Storage And Preservation

    Chlorine To remove chlorine from a sample, add one powder pillow of sodium thiosulfate (Hach 1436369 dechlorination agent) to 20 mL of sample and wait for 10 minutes. High oxygen Causes light inhibition that is not caused by toxicity consumption pH-related light inhibition may occur if the pH is below 6.0 or above...

  • Page 60: Prepare The Sample

    Interfering Interference levels and treatments substances A sodium chlorine (NaCl) concentrations of less than 15 g/L or more than 50 g/L (or their osmolarity equivalents) in a sample will cause osmosis-related light inhibition. Sodium chloride The addition of solid NaCl to the sample (2% final concentration), prevents osmosis-related light inhibition of samples of low or unknown NaCl concentrations.

  • Page 61: Prepare The Test Tubes

    Solid NaCl is used to change the sample osmolarity to a value that is correct for the marine bacterium used in the test. If the sample has a high toxicity, carry out a preliminary dilution of the sample with 2% NaCl solution. Select a preliminary dilution from the levels 1:2, 1:4, 1:8, 1:16, etc.
  • Page 62 4. Put the tip of the pipette 5. Slowly dispense the test 6. Do steps again into the test suspension suspension into the test until all four test tubes and slowly pull in 0.2 mL. tube in position 1. contain 0.2 mL of test suspension 7.
  • Page 63 13. Set the 0.2 - 1.0 mL 14. Put the tip of the pipette 15. Slowly dispense the 2% pipette to 0.3 mL. into the 2% NaCl and NaCl solution into the test slowly pull in 0.3 mL. tube in position 3. Note: No 2% NaCl is put in the test tube in position 4.

  • Page 64: Measure The Toxicity Of The Sample Dilutions

    20 °C, the initial light made by the bacteria can be more than 1000 Eclox light units. This causes the error Detector Overload. If an error occurs, change the measurement range from the 0–1000 range to the 0–2000 range and...
  • Page 65 To measure the toxicity of the sample dilutions: Push ON (green button) for several seconds to apply power to the luminometer. When the built-in tests are done, push PROCEED. The Main Menu is shown. Select Luminescent Bacteria Test and push ENTER. Select Measure and push ENTER.
  • Page 66 Luminometer is shown, select LIMIT Measure and Send to PC and push ENTER. The luminometer must be connected to a computer (refer to Connect the luminometer to a computer on page 23). • To measure the luminescence and print the results on a printer, select LIMIT Measure and Send to Printer and push ENTER.
  • Page 67 12. Open the lid of the 13. Do steps 22 to 24 again 14. Use the color chart on luminometer. to measure the three other the Screening test tubes in the correct Luminescence Results Remove the test tube from order (2, 3, and then 4). Sheet to identify which the luminometer and put it sample dilutions are toxic...
  • Page 68 Luminescent Bacteria Toxicity Test - Screening Luminescence Results Sample: _______________________________________________________ Date: _______________ Time: _______________ Operator: ______________________________________________________ Comments: Procedure: 1. Add 1.0 mL of reconstitution solution to the reagent. Swirl to mix. Wait 5 minutes. 2. Add 1.0 mL of stock suspension to 10 mL of Diluent.

  • Page 69: Show Or Send Previous Results

    10.13 Show or send previous results To show previous results on the luminometer, do the procedure in this section for the type of procedure done. To send previous results to a computer: Note: At this stage, the results cannot be sent to the LUMISsoft 4. Do the steps in Connect the luminometer to a computer on page Start LUMISsoft.

  • Page 70: Description Of Limit Measure Results

    10.13.2 Description of LIMIT measure results The LIMIT measure procedure results are recorded the same as the screening luminescence results. The only difference is that the LIMIT measure results include a column that shows whether the percentage inhibition calculated for each sample measurement is above the LIMIT value or below the LIMIT value set by the user as shown in Figure...

  • Page 71: Show Or Send Limit Measure Results

    • To show the results on the luminometer, select Show all (R1 to Rx) and push ENTER. • To send the results to the computer, select Send all (R1 and Rx) to PC and push ENTER. • To send the results to the printer, select Send all (R1 to Rx) to Printer and push ENTER.
  • Page 72 The Previous LIMITs Menu is shown. To show all or send all of the results saved on the luminometer, select one option: • To show the results on the luminometer, select Show all (R1 to Rx) and push ENTER. • To send the results to the computer, select Send all (R1 and Rx) to PC and push ENTER.

  • Page 73: Section 11 Luminescent Bacteria Toxicity Test Iso 11348 Part 3

    This chapter describes the LBT Test measurement luminescence procedure and contains the procedure steps. Use the LBT Test measurement luminescence procedure if the test needs to be done according to ISO 11348 part 3. The Eclox LBT Test measurement luminescence procedure meets the criteria of validation of ISO 11348-3.

  • Page 74: Accuracy

    11.3 Thermostat and PC software requirements ISO 11348 states that the measuring luminometer must have a 15 °C temperature controlled measuring well. The Eclox does not have a temperature controlled measuring cell. According to ISO 11348 optional accessories that should be used in the lab include: •...

  • Page 75: Reagent Storage And Preservation

    11.5 Reagent storage and preservation The freeze-dried reagent can be kept at -18 °C until the expiration date shown on the package. Tubes that contain thawed but not reactivated freeze-dried luminescent bacteria can be frozen again and kept on stock. The reagent can be transported or shipped up to 7 days at no more than 25 °C.
  • Page 76 This procedure is temperature sensitive. 1. Remove the 2. Remove the cap from 3. Remove the foil seal and luminescent bacteria test the reconstitution solution rubber stopper from the reagent from the freezer. bottle. reagent bottle. Remove the reconstitution solution from refrigerator. 4.

  • Page 77: Prepare The Test Suspension

    11.6.2 Prepare the test suspension Prepare enough test suspension (stock suspension and Diluent mixture) to do the test. Each test tube used for the test is filled with 0.5 mL of test suspension. To identify the number of test tubes used for a test: •...
  • Page 78 4. Put 1 part stock 5. Put the cap on the 6. Put one half of the new, suspension (S) at reaction vessel and shake empty test tubes in Row B refrigerator temperature to mix thoroughly. and one half of the test into a clean reaction vessel tubes in Row C of the using a pipette.
  • Page 79 13. Remove the pipette tips from the pipettes and put in the waste bag. Put the pipettes in the storage case. 11.6.2.2 Test suspension for D 1 values Prepare the test suspension for D1 values if the sample is probably non-toxic to measure the sample toxicity using the highest possible sample concentration of 80% (= D 1).
  • Page 80 4. Put 1 part stock 5. Put the cap on the 6. Put 50 parts Diluent suspension (S) at reaction vessel and shake solution (D) at refrigerator refrigerator temperature to mix thoroughly. temperature into a clean into a clean reaction vessel reaction vessel using a Put a “1:20”...
  • Page 81 10. Set the 0.2 - 1 mL 11. Put the end of the 12. Put the tip of the pipette to 0.2 mL. pipette into a clean pipette pipette into the reaction tip. vessel that contains the 1:20 test suspension and slowly pull in 0.2 mL of the test suspension.
  • Page 82 16. Put the tip of the 17. Slowly dispense the 18. Do step pipette into the reaction test suspension into the again until each test tube in vessel that contains the test tube in position B3. Row B and Row C (position 1:50 test suspension and 3 and higher) contains 0.5 slowly pull in 0.5 mL of the...

  • Page 83: Sample Collection, Storage And Preservation

    Chlorine To remove chlorine from a sample, add one powder pillow of sodium thiosulfate (Hach 1436369 dechlorination agent) to 20 mL of sample and wait for 10 minutes. High oxygen Causes light inhibition that is not caused by toxicity consumption pH-related light inhibition may occur if the pH is below 6.0 or above...

  • Page 84: Prepare The Sample

    11.9 Prepare the sample To prepare the sample for testing: If the sample is turbid, either: • Filter the sample with a modified polysulfone filter Before using other filter materials, test the filter material with 2% NaCl first to make sure that the filter material can be used with the Luminescent Bacteria Toxicity Test.

  • Page 85: Prepare The Dilutions Series

    11.10 Prepare the dilutions series Prepare the sample dilutions series using one of the procedures in this section. The sample dilutions are added to the test suspension later to identify the percent inhibition of each sample dilution. A non-toxic reference is added to the test suspension during the test and measured.
  • Page 86 1. Put 10 empty test tubes 2. Set the 1.0 - 5.0 mL 3. Put the end of the pipette into Row A of the pipette to 1.0 mL. into a clean pipette tip. LUMIStherm. 4. Put the tip of the pipette 5.
  • Page 87 9. Set the 1.0 - 5.0 mL 10. Put the tip of the pipette 11. Slowly dispense the pipette to 2.0 mL. into the sample and slowly sample into the test tube in pull in 2.0 mL. position A9. 12. Set the 1.0 - 5.0 mL 13.
  • Page 88 18. Pull in 1.5 mL of 19. Pull in 1.5 mL of 20. Pull the solution in A8 solution from A7 and put it solution from A5 and put it into the pipette 2 to 3 times into A5 using the pipette. into A3 using the pipette.

  • Page 89: Prepare A 3 Dilution Series (D 2 Values And Higher)

    24. Keep the dilution series at 15 °C for at least 5 minutes to correct the temperature. Figure 7 Before the dilution series is started - 9 dilutions, D 2 and higher 1 Sample 2 2% NaCl solution 11.10.2 Prepare a 3 dilution series (D 2 values and higher) To make a 3 dilution series according to ISO 11348 of D 2 sample values and higher, do this procedure.
  • Page 90 Figure 8 shows the contents of the test tubes in Row A at the end of this procedure Figure 8 Dilution series - 3 dilutions, D 2 values and higher 1 Sample 2 2% NaCl solution 1. Put 4 empty test tubes 2.
  • Page 91 7. Put the tip of the pipette 8. Slowly dispense the 2% 9. Do steps againto into the 2% NaCl solution NaCl solution into the test put 1.5 mL of 2% NaCl and slowly pull in 1.5 mL. tube in position A2. solution into the test tube in position A1.
  • Page 92 16. Do steps 17. Keep the dilution series againto put 1.5 mL of the at 15 °C for at least 5 sample into the test tube in minutes to correct the position A2. temperature.

  • Page 93: Prepare A 9 Dilution Series (D 1 Values And Higher)

    11.10.3 Prepare a 9 dilution series (D 1 values and higher) To make a 9 dilution series according to ISO 11348 of D 1 sample values and higher, do this procedure. This procedure makes dilutions ranging from undiluted to a dilution ratio of 1:8 in Row A.
  • Page 94 1. Put 10 empty test tubes 2. Set the 1.0 - 5.0 mL 3. Put the end of the pipette into Row A of the pipette to 1.0 mL. into a clean pipette tip. LUMIStherm. 4. Put the tip of the pipette 5.
  • Page 95 10. Put the tip of the pipette 11. Slowly dispense the 2% 12. Do steps into the 2% NaCl solution NaCl solution into the test againto put 1.5 mL of 2% and slowly pull in 1.5 mL. tube in position A8. NaCl solution in each test tube in positions A7, A6, A5, A4 and A3 .
  • Page 96 19. Put the tip of the pipette 20. Slowly dispense the 21. Do steps into the sample and slowly sample into the test tube in againto put 1.5 mL of the pull in 1.5 mL. position A8. sample into the test tube in position A10.
  • Page 97 25. Pull the solution in A8 26. Pull in 1.5 mL of 27. Pull in 1.5 mL of into the pipette 2 to 3 times solution from A8 and put it solution from A6 and put it to mix the sample dilution. into A6 using the pipette.

  • Page 98: Prepare A 3 Dilutions Series (D 1 Values And Higher)

    11.10.4 Prepare a 3 dilutions series (D 1 values and higher) To make a 3 dilution series according to ISO 11348 of D 1 sample values and higher, do this procedure. This procedure makes dilutions ranging from undiluted to a dilution ratio of 1:1.5 in Row A.
  • Page 99 4. Put the tip of the pipette 5. Slowly dispense the 2% 6. Set the 1.0 - 5.0 mL into the 2% NaCl solution NaCl solution into the test pipette to 1.5 mL. and slowly pull in 1.0 mL. tube in position A4. 7.
  • Page 100 13. Put the tip of the pipette 14. Slowly dispense the 15. Do steps into the sample and slowly sample into the test tube in againto put 2.0 mL of pull in 2.0 mL. position A4. sample into the test tube in position A2.

  • Page 101: Measure The Light Intensity Of The Test Suspension

    20 °C, the initial light made by the bacteria can be more than 1000 Eclox light units. This causes the error Detector Overload. If an error occurs, change the amplification settings from 0–1000 to 0–2000 light units and do the...
  • Page 102 1. Open the luminometer 2. Push PROCEED to 3. If testing D 2 values and lid and remove any sample show the test status. higher, set the 0.2 - 1.0 mL that is in the cell. Close the pipette to 0.5 mL. When the cell tests are lid.
  • Page 103 7. While B1 is being 8. Record the measured 9. Open the lid of the measured, set the timer to value on a sheet of paper if luminometer. the correct contact time the measured value is Remove the test tube from (e.g., 15 or 30 minutes).
  • Page 104 13. When the C1 14. Open the lid of the 15. Put the test tube in measurement is done, luminometer. position B2 into the black record the measuring value test tube holder in the Remove the test tube from on a sheet of paper if the luminometer cell.
  • Page 105 Note: Add the sample dilution 19. Open the lid of the 20. Do steps to each test tube in Row B and luminometer. again until: Row C immediately after the test tube is measured. Remove the test tube from • All the test tubes in Row the luminometer and put it B and C have been back in the LUMIStherm.

  • Page 106: Measure The Light Intensity Of The Test Suspension After The Sample Dilutions Are Added

    11.12 Measure the light intensity of the test suspension after the sample dilutions are added Measure the light intensity of the test suspension (luminescent bacteria) after the sample dilutions are added according to ISO 11348 using this procedure. The Luminescent Bacteria Toxicity Test is a biological test method and the result is therefore strongly temperature-dependent.
  • Page 107 4. Close the luminometer 5. Record the measured 6. Open the lid of the lid. Push MEASURE. value on a sheet of paper if luminometer. the measured value is When the measurement is Remove the test tube from shown but not saved to the complete (approximately the luminometer and put it luminometer, saved to the...

  • Page 108: Show Or Send Previous Results

    11.13 Show or send previous results Measured luminescent procedure values are stored by an indicator counter M1 to Mx (refer to Figure 12). The counter starts with M1 every time the storage is erased from the luminometer. Figure 12 Example measurement luminescence results To show previous results on the luminometer for the Luminescent Bacteria Test (LBT), do the procedure in this section.
  • Page 109 The Previous Results Menu is shown. Select Show Previous Measurements and push ENTER. The Previous Measurements Menu is shown. To show all or send all of the results saved on the luminometer, select one option: • To show the results on the luminometer, select Show all (R1 to Rx) and push ENTER.

  • Page 111: Section 12 Maintenance

    If the Eclox Rapid Response Test Kit comes into contact with any chemical warfare (CW) agent, decontaminate the kit before it is used again. When the carrying case for the Eclox Rapid Response Test Kit is closed, the kit is waterproof and can be sprayed/wetted. The case is chemically made hard.

  • Page 112: Battery Replacement

    12.3 Battery replacement 12.3.1 Luminometer battery replacement Remove any excess water from the surface of the luminometer. Measurement errors will occur if water gets into the meter. Remove the battery cover of the luminometer with the Battery Cover Screw Tool. Remove the batteries from the luminometer and dispose of them in accordance with local operating procedures.
  • Page 114 Visit us at www.hach.com...

  • Page 115: Section 13 Troubleshooting

    Section 13 Troubleshooting Table 4 Luminometer troubleshooting Display Fault Corrective action Change the measurement range to the 0–2000 — Detector overload range (refer to section 3.2.3, Set the measurement range on page 18). Change batteries (refer to section 12.3.1, — cannot read the display Luminometer battery replacement on page 112).
  • Page 116 Remove power and then apply power to the Cannot store measurements luminometer. If fault occurs again, contact technical support. Remove power and then apply power to the Initialization fault luminometer. If fault occurs again, contact technical support. Visit us at www.hach.com...

  • Page 117: Section 14 Replacement Parts And Accessories

    Eclox Replacement Reagent Set (includes 50 chemiluminescent reagent sets, 25 pesticide/ nerve agent tests, 50 free chlorine, 50 total chlorine, 100 Hach Arsenic each 2886900 Tests, 10 pH buffer (Singlets) 4.01 from Hach and 10 pH buffer (Singlets) 7.00 from Hach)
  • Page 118 Replacement parts (continued) Description Item number Arsenic Test Cap, Arsenic Test Kit each 4934800 Cotton balls 257201 EZ Arsenic Reagent #1 each 2822999 EZ Arsenic Reagent #2 each 2823099 EZ Arsenic Reagent Set (Reagent #1 and #2) each 2823200 Lead acetate, 100 mL each 1458042 Reaction bottle, arsenic...
  • Page 119 Luminescent Bacteria Accessories Kit (includes case, reconstitution solution, dilution solution, 2% NaCl solution, NaCl solid in a bottle with a dosing spoon, plastic test tubes for Eclox, reaction vessels, rack for 8 reaction each LCW490 vessels, stand for 40 test tubes, variable pipette 0.2-1.0 mL, variable pipette 1.0-2.0 mL, pipette tips and timer...

  • Page 121: Luminescent Bacteria Risks

    Appendix A Luminescent bacteria risks This appendix contains risk assessment information for Photobacterium fisheri (synonym: Vibrio fischeri) manufactured by HACH-LANGE GmbH in Germany. The Luminescent Bacteria Toxicity Test reagent contains freeze-dried or liquid-dried Photobacterium fisheri bacteria. Photobacterium fisheri luminescent bacteria are well known as non-pathogenic and innocuous.

  • Page 122: Disposal

    characterized strains of viable microorganisms that are not known to consistently cause disease in healthy adult humans. Bacillus subtilis, Naegleria gruberi, infectious canine hepatitis virus, and exempt organisms under the NIH Recombinant DNA Guidelines are representative of microorganisms meeting these criteria.
  • Page 124 Fax (970) 669-2932 Fax +49 (0) 2 11 52 88-210 Tel. +41 22 594 6400 orders@hach.com info-de@hach.com Fax +41 22 594 6499 www.hach.com www.de.hach.com © Hach Company/Hach Lange GmbH, 2004, 2006, 2010, 2013, 2018. All rights reserved. Printed in U.S.A.

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