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Agilent Technologies 490 User Manual

Micro gas chromatograph
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Summary of Contents for Agilent Technologies 490

  • Page 1 Agilent 490  Micro Gas Chromatograph User Manual...
  • Page 2 Further, to the max- agreement and written consent from  imum extent permitted by applicable Agilent Technologies, Inc. as governed by C A U T I O N law, Agilent disclaims all warranties, United States and international copyright either express or implied, with regard laws.

  • Page 3: Table Of Contents

    Carrier Gas Connection Sample Gas Handling a sample Using the external filter unit Heated sample lines Connecting to a heated sample line Power Power source Power Requirements Disposal Specifications Ambient Pressure Ambient Temperature Maximum Operation Altitude 490 Micro GC User Manual...
  • Page 4 Pre-Installation Requirements Inspect the Shipping Packages Unpack the Micro GC Review the Packing List Install the 490 Micro GC Step 1: Install gas regulators and set pressures Step 2: Connect carrier gas Step 3: Connect to the checkout sample Step 4: Connect to power...
  • Page 5 To change the Micro GC network settings Frequently Asked Questions (FAQ) Glossary of network terms External Digital I/O External Analog I/O Micro GC Cycle with Constant Pressure Micro GC Cycle with Ramped Pressure Errors Error Handling Error List 490 Micro GC User Manual...
  • Page 6 490 Micro GC User Manual...

  • Page 7: Introduction

    Instrument Disposal This chapter provides important information about using the Agilent 490 Micro Gas Chromatograph (Micro GC) safely. To prevent any injury to you or any damage to the instrument it is essential that you read the information in this chapter.

  • Page 8: Safety Information

    • Hydrogen has a very low ignition energy. • Hydrogen that is allowed to expand rapidly from high pressure into the atmosphere can self-ignite. • Hydrogen burns with a nonluminous flame which can be invisible under bright light. 490 Micro GC User Manual...

  • Page 9: Safety Symbols

    Failure to comply with these precautions violates safety standards of design and the intended use of the instrument. Agilent Technologies assumes no liability for the customer’s failure to comply with these requirements.

  • Page 10: General Safety Precautions

    If the equipment is used in a manner not specified by the manufacturer, the protection provided by the equipment may be impaired. 490 Micro GC User Manual...
  • Page 11 • When working with this instrument, follow the regulations for Good Laboratory Practices (GLP). Take care to wear safety glasses and appropriate clothing. • Do not place containers with flammable liquids on this instrument. Spilling liquid over hot parts may cause fire. 490 Micro GC User Manual...
  • Page 12 • Substituting parts or performing any unauthorized modification to the instrument may result in a safety hazard. • Changes or modifications not expressly approved by the responsible party for compliance could void the user's authority to operate the equipment. 490 Micro GC User Manual...

  • Page 13: Shipping Instructions

    Do not use compressed air to clean the instrument. Instrument Disposal When the Micro GC or its parts have reached the end of their useful life, dispose of them in accordance with the environmental regulations that are applicable in your country. 490 Micro GC User Manual...
  • Page 14 Introduction 490 Micro GC User Manual...

  • Page 15: Instrument Overview

    Ambient Pressure Ambient Temperature Maximum Operation Altitude There are several versions of the Agilent 490 Micro GC. All of them use GC channels, each of which consists of an Electronic Gas Control (EGC) injector, column, and detector. The Micro GC is a self-contained package with all of the normal GC components.

  • Page 16: Front View

    Sample 1 and Sample 2 Sample gas inlet connector (for unheated front inlets) “Sample Gas” on page 23 Power On/Off Switch Switch the Micro GC ON or OFF Figure 1 Front view of the 490 Micro GC 490 Micro GC User Manual...

  • Page 17: Back View

    Carrier gas input Carrier gas input connector “Carrier Gas Connection” page 21 Power connector Power connector (male) “Power” on page 26 Figure 2 Back view of the 490 Micro GC (shown with shipping caps in place) 490 Micro GC User Manual...

  • Page 18: Inside View

    Digital input and output External analog I/O signals. signals, such as start_stop, “External Analog ready_out, and start_in. I/O” on page 75. “External Digital I/O” on page 74. Figure 3 Cable connectors (original main board CP740010 shown) 490 Micro GC User Manual...
  • Page 19 External analog I/O signals. Digital input and output “External Analog signals, such as start_stop, I/O” on page 75. ready_out, and start_in. “External Digital I/O” on page 74. Figure 4 Cable connectors (main board G3581-65000 shown) 490 Micro GC User Manual...
  • Page 20 - start out;  ‡ extension boards extension boards extension boards Not available Not available Not available * Optional PRO license required † LCD display not included ‡ Extension boards not included **Extension boards not included 490 Micro GC User Manual...

  • Page 21: Carrier Gas Connection

    Table 2 Relative thermal conductivities Relative thermal Relative thermal conductivities conductivities Carrier gas Carrier gas Hydrogen 47.1 Ethane Helium 37.6 Propane Methane Argon Oxygen Carbon dioxide 4.4 Nitrogen Butane Carbon monoxide 490 Micro GC User Manual...
  • Page 22 Hydrogen is flammable. If you are using hydrogen as a carrier gas, WA R N I N G pay particular attention to possible leaks at connections inside and outside of the Micro GC (use an electronic leak tester). 490 Micro GC User Manual...

  • Page 23: Sample Gas

    24. Orient the arrow on the female half of the filter towards the fingertight fitting. Replace the external filter unit at regular intervals. See “Review the Packing List” on page 32 for part numbers. 490 Micro GC User Manual...

  • Page 24: Heated Sample Lines

    A heated injector and sample line is an option for a channel unit, and is used to prevent sample from condensing in the sample lines when analyzing condensable samples. The heated sample and injector can be controlled between 30 °C and 110 °C. 490 Micro GC User Manual...

  • Page 25: Connecting To A Heated Sample Line

    Removing the insulation Figure 8 Sample line connector Connect the sample line Insulate the sample line coming into the Micro GC to prevent damage to C A U T I O N any communications cables. 490 Micro GC User Manual...

  • Page 26: Power

    Micro GC. See Table 3 on page 27 for specifications. Figure 9 Model FRA180-S120-4 (P/N CP742999) Disposal Disposal of the Power Supply must be carried out in accordance with all environmental regulations applicable in your country. 490 Micro GC User Manual...

  • Page 27: Specifications

    The Micro GC automatically shuts down if the ambient pressure is greater than 120 kPa. Ambient Temperature The Micro GC automatically shuts down if the ambient temperature exceeds 65 °C. Maximum Operation Altitude The maximum operation altitude is 2000 meters. 490 Micro GC User Manual...
  • Page 28 Instrument Overview 490 Micro GC User Manual...

  • Page 29: Installation And Use

    Agilent 490 Micro Gas Chromatograph User Manual Installation and Use Pre-Installation Requirements Inspect the Shipping Packages Unpack the Micro GC Review the Packing List Install the 490 Micro GC Create the Test Method Perform a Series of Runs Shut Down Procedure Long Storage Recovery Procedure This chapter describes how to install and use the instrument.

  • Page 30: Pre-Installation Requirements

    The Micro GC will arrive in one large box and one or more smaller cartons. Inspect the cartons carefully for damage or signs of rough handling. Report damage to the carrier and to your local Agilent office. 490 Micro GC User Manual...

  • Page 31: Unpack The Micro Gc

    The instrument has been protected during shipment by protective caps. C A U T I O N Figure 10. Before use, remove these caps, including those on the back panel. Protective shipping caps Figure 10 Protective shipping caps 490 Micro GC User Manual...

  • Page 32: Review The Packing List

    Tubing,SS,pre-tsd,1/16in. CP4008 ODx1.0mmID,1/p Tubing, SS,1/16in. ODx1.0mmID,1 mL,1/p CP4009 0.080 Fingertight Fitting PEEK CP23050 5 FILTERS for EXT. FILTER Assembly CP736467 External Filter Male CP736737 CP736736 External Filter FeMale Micro GC power supply, 12V, 150W CP742999 490 Micro GC User Manual...

  • Page 33: Install The 490 Micro Gc

    Installation and Use Install the 490 Micro GC If you are installing the 490 Micro GC for the first time, follow the steps as described below. If you are performing a re-installation, see “Long Storage Recovery Procedure” on page 43.

  • Page 34: Step 5: Connect To The Data System Computer (Or Lan)

    Make sure the Micro GC is switched off. Be sure the Micro GC is connected directly to a PC using a crossover cable. Start up the Micro GC Service tool. The Micro-GC Communication Setup screen opens. 490 Micro GC User Manual...
  • Page 35 Installation and Use Figure 11 Micro GC Communication Setup screen In the Micro-GC Communication Setup screen, click Setup IP Address to open the Setup Ethernet Connection window. Figure 12 Network webpage 490 Micro GC User Manual...
  • Page 36 After receiving the IP address, the Micro GC will continue its startup sequence by blinking all LEDs in sequence. The Service Tool responds with a Confirmation message that the IP address was assigned (see Figure 14). 490 Micro GC User Manual...

  • Page 37: Step 6B: Assign An Ip Address - For A Micro Gc With Main Board G3581-65000

    IP address configured. The active IP address is specified on the sticker together with the MAC address and the main board serial number (see Table Table 5 Factory default IP address settings Default IP address 192.168.100.100 Subnet mask 255.255.255.0 microgc Host name Default Gateway N/A (not used) 490 Micro GC User Manual...
  • Page 38 Micro GC in the address field of the web browser. On the web page, click Network. Log in as administrator. Use the factory default login and password: • Login name: admin • Password: agilent 490 Micro GC User Manual...
  • Page 39 In the network webpage, the upper section shows the current IP configuration. Type the IP Address, Subnet mask, and Gateway you want to assign to the Micro GC in the corresponding fields. Figure 17 Micro GC website 490 Micro GC User Manual...

  • Page 40: Step 7: Note The Micro Gc Startup Cycle

    Especially make sure the carrier gas types match the gas actually supplied to the Micro GC. Start the Micro GC’s online instrument session. Refer to the data system online Help and User Manuals for more details if necessary. 490 Micro GC User Manual...

  • Page 41: Create The Test Method

    Monitor the instrument status using the data system’s status display (refer to the data system help for details). Each test method has been designed to determine if the instrument channel is functioning properly and includes an example test chromatogram. 490 Micro GC User Manual...

  • Page 42: Perform A Series Of Runs

    Perform a Series of Runs Create a short sequence of at least three runs using the test sample and method. Run the sequence. After the first run, the results for each channel should become similar to the example chromatograms. 490 Micro GC User Manual...

  • Page 43: Shut Down Procedure

    Micro GC. Refer to the Site Preparation Guide for supply pressures and other gas requirements. Wait at least 10 minutes before switching ON the Micro GC. Immediately check if the detector filaments are switched OFF. Switch OFF if necessary. 490 Micro GC User Manual...
  • Page 44 (160 °C or 180 °C depending on the column limit). Condition the GC column, preferably overnight. This will ensure that all the water has been removed from the column module and no damage will occur to the TCD filaments. 490 Micro GC User Manual...

  • Page 45: Gc Channels

    Agilent 490 Micro Gas Chromatograph User Manual GC Channels Carrier Gas Micro Electronic Gas Control (EGC) Injector Column Backflush Option TCD Detector The instrument contains up to 2 channels in a dual channel cabinet, or up to 4 channels for a quad channel cabinet. A GC channel contains a gas regulator, an injector, a column, and a TCD detector.

  • Page 46: Carrier Gas

    Use the carrier gas type for which your instrument is configured, otherwise the detector filaments can be damaged. Columns (analytical and Microelectronic Gas Clean unit reference) gas control (optional) (EGC) Injector µTCD Reference vent Column vent Sample Out Sample Carrier gas Figure 18 Gas flow diagram 490 Micro GC User Manual...

  • Page 47: Micro Electronic Gas Control (Egc)

    (ms). This equals an average injection volume of 200 nL. Injection time will be rounded to a multiple of 5 ms. A practical minimum value is 40 ms. A value of 0 to 20 milliseconds might result in no injection. 490 Micro GC User Manual...

  • Page 48: Column

    180 °C, the maximum temperature of the column oven. Exceeding this temperature will cause the column to lose efficiency instantly and the column module will need replacement. All channels have a built-in protection that prevents a setpoint above the maximum tempera- ture. 490 Micro GC User Manual...

  • Page 49: Molsieve 5Å Columns

    Argon 0.2% Argon 0.2% Peak 4 _________ Oxygen 0.2% Oxygen 0.2% Peak 5 _________ Nitrogen 0.2% Nitrogen 0.2% Molsieve 5Å 4 m heated Molsieve 5Å 10 m unheated Molsieve 5Å 20 m unheated Seconds seconds Seconds 490 Micro GC User Manual...

  • Page 50: Cp-Sil 5 Cb Columns

    Ethane 8.1% Peak 3 Propane 1.0% Propane 1.0% Peak 4 i-Butane 0.14% i-Butane 0.14% Peak 5 n-Butane 0.2% n-Butane 0.2% CP Sil 5 CB 4 m heated CP Sil 5 CB 6 m unheated Seconds Seconds 490 Micro GC User Manual...

  • Page 51: Cp-Sil Cb Columns

    TBM 6.5 ppm THT 4.6 ppm Peak 3 ________ Noane 4.5 ppm CP Sil 13 CB CP Sil 19 CB 12 m heated (TBM) 6 m unheated (THT) -0.5 -0.2 -0.4 -1.5 -0.6 -0.8 Seconds Seconds 490 Micro GC User Manual...

  • Page 52: Poraplot 10M Column

    Detector sensitivity Auto Auto Peak 1 Composite Balance Peak 2 Ethane 8.1% Peak 3 Propane 1.0% Peak 4 i-Butane 0.14% Peak 5 n-Butane 0.2% PoraPlot U 10 m heated PoraPlot Q 10 m heated Seconds Seconds 490 Micro GC User Manual...

  • Page 53: Hayesep A 40 Cm Heated Column

    150 kPa (21 psi) Sample time 30 s Injection time 40 ms Run time 60 s Detector sensitivity Auto Peak 1 Nitrogen 0.77% Peak 2 Methane Balance Peak 3 Ethane 8.1% Hayesep A 40 cm heated Seconds 490 Micro GC User Manual...

  • Page 54: Cox And Al203/Kci Columns

    Peak 2 Nitrogen 1.0% Ethane 8.1% Peak 3 CO 1.0% Propane 1.0% Peak 4 Methane 1.0% i-Butane 0.14% Peak 5 1.0% n-Butane 0.2% Helium Balance 1 m unheated /KCl 10 m heated -0.5 Seconds Seconds 490 Micro GC User Manual...

  • Page 55: Mes (Nga) And Cp-Wax 52 Cb Columns

    MES 14. 2 ppm Methanol 0.15% Peak 4 ________ Ethanol 0.30% Helium Balance MES 10 m heated (NGA) CP-WAX 52 CB 4 m heated 4000 3500 3000 2500 2000 -0.3 1500 1000 -500 Seconds Seconds 490 Micro GC User Manual...

  • Page 56: Column Conditioning

    If the Micro GC frequency of use is very high, you might consider routinely leaving the oven temperature at 180 °C overnight. The longer the reconditioning period, the better the column performance. 490 Micro GC User Manual...

  • Page 57: Backflush Option

    58. The injector, two columns, and detector are in series. The sample is injected onto the pre-column where a pre-separation takes place; injection takes place in normal mode. See Figure 20 on page 58. 490 Micro GC User Manual...
  • Page 58 Backflushing saves the time required to elute high boiling components that are not of interest and ensures that the pre-column will be in good condition for the next run. 490 Micro GC User Manual...

  • Page 59: Tuning

    The Backflush Time range is from 0.5 seconds until the maximum run time. To disable backflush To disable backflushing, set the Backflush Time to 0. This puts the system in normal mode during the entire run. 490 Micro GC User Manual...

  • Page 60: Tcd Detector

    (carrier gas containing sample components). The construction of a TCD is such that the changing thermal conductivity of the carrier gas stream, due to components present, is compared to the thermal conductivity of a constant reference gas stream. 490 Micro GC User Manual...

  • Page 61: Communications

    Agilent 490 Micro Gas Chromatograph User Manual Communications Access the Connection Ports 490 Chromatography Data Systems Ethernet Networks External Digital I/O External Analog I/O Micro GC Cycle with Constant Pressure Micro GC Cycle with Ramped Pressure This chapter describes the input and output ports accessible inside the Micro GC for interfacing with external devices.

  • Page 62: Access The Connection Ports

    22). Figure 22 Instrument cover At the front of the instrument, the external device connectors are visible (Figure 23). Figure 23 External device connectors (Main board CP740010 shown) Close the cover after connecting the cables. 490 Micro GC User Manual...

  • Page 63: 490 Chromatography Data Systems

    Communications 490 Chromatography Data Systems The 490 Micro GC requires an Agilent chromatography data system (CDS) for control, peak identification, integration, data analysis, reporting, and so forth. See Table 15. The CDS requires a LAN (Ethernet) connection. Multiple Micro GCs can be controlled using an Agilent data system such as EZChrom, OpenLAB EZChrom Edition, or OpenLAB Chemstation Edition.

  • Page 64: Ethernet Networks

    (LAN) or wide area network (WAN). General requirements: • Micro GC with main board CP740010 installed (10 Mbps connection) • Cat5 or Cat3 UTP/STP cabling. • The network should comply with Standard Ethernet (IEEE 802.3). 490 Micro GC User Manual...

  • Page 65: Ip Addresses

    “private” ranges shown in Table Table 16 Private (isolated) LAN IP address ranges Starting IP Ending IP Subnet mask Type 0.0.0.0 255.255.255.255 Public 10.0.0.0 10.255.255.255 255.0.0.0 Private 172.16.0.0 172.31.255.255 255.255.0.0 Private 192.168.0.0 192.168.255.255 255.255.0.0 Private 490 Micro GC User Manual...

  • Page 66: Example Network Configurations

    (non-crossed) patch cable can be used. Crossover cable Data system PC Any IP address allowed Micro GC with main board CP740010 Single 490 connected to a local workstation. IP address in the same subnet range as the computer subnet range. Crossover cable or...
  • Page 67 Switch Patch cable Data system PC Any IP address allowed Multiple 490 Micro GCs connected to a local workstation. IP address in the same subnet range as the workstation subnet range. Figure 26 Local network (multiple instruments) OpenLAB CDS maximum connections are limited by the computer speed, license, and network performance.
  • Page 68 Patch cable Data system PC IP address in subnet range of Subnet 1 Can also control Micro GC in Subnet 2. Multiple 490 Micro GCs connected t Router company network. IP address in the same subnet range as Subnet 1.

  • Page 69: To Restore The Factory Default Ip Address

    5). Sometimes it is necessary to restore this IP address if communication with the Micro GC is lost. The 490 Micro GC with main board G3581-6500 has a reset button that can be accessed at the back of the instrument. See Figure 28.

  • Page 70: To Change The Micro Gc Network Settings

    Micro GC in the address field of the web browser. On the web page, click Names. Log in as administrator. Use the factory default login and password: • Login name: admin • Password: Agilent Click the items you would like to change. 490 Micro GC User Manual...
  • Page 71 If one or more DNS servers and/or search domain are required, type in the DNS IP addresses (space separated) and search domain. These settings are not required for correct operation of the Micro GC. Click Save to save settings. Power cycle the Micro GC. 490 Micro GC User Manual...

  • Page 72: Frequently Asked Questions (Faq)

    Domain One of several settings within the TCP/IP configuration that identifies paths used to communicate with Ethernet devices. The Domain is an IP address. Ethernet address (MAC address) This is a unique identifier that every Ethernet communication device has assigned to it. 490 Micro GC User Manual...
  • Page 73 Micro GC. TCP/IP An international standard protocol used by the Internet. We use this protocol for communication to the Micro GC. You may find several network protocols, such as IPX/SPX and NetBEUI, installed on your computer. 490 Micro GC User Manual...

  • Page 74: External Digital I/O

    Reserved +5 VDC out (electronic fused max 500 mA ) +12 VDC out ( electronic fused max 500 mA ) External Digital I/O Relay contacts maximum 24 Volt 1 Ampere Figure 30 External digital connections 490 Micro GC User Manual...

  • Page 75: External Analog I/O

    -15 VDC out (electronic fused max 100 mA ) +5 VDC out (electronic fused max 500 mA ) +12 VDC out (electronic fused max 500 mA ) Analog GND External analog I/O Figure 31 External analog connections 490 Micro GC User Manual...

  • Page 76: Micro Gc Cycle With Constant Pressure

    This time allows acquisition starts. run is finished. must be downloaded The data system will wait the sample to via the data system. until data is received settle in the from the Micro GC. sample loop. 490 Micro GC User Manual...

  • Page 77: Micro Gc Cycle With Ramped Pressure

    final column head has been time begins. The pressure from the returned to the pressure remains final pressure to initial pressure. the same. the initial Fixed at 500 mS. pressure. 490 Micro GC User Manual...
  • Page 78 Communications 490 Micro GC User Manual...

  • Page 79: Errors

    Agilent 490 Micro Gas Chromatograph User Manual Errors Error Handling Error List Agilent Technologies...

  • Page 80: Error Handling

    (for troubleshooting). All Class 1 and higher errors are also logged in the instrument’s flash memory. Individual numbers identify all errors; these numbers are built using the error class and a number. Events are not numbered. 490 Micro GC User Manual...
  • Page 81 TCD off and or download. temp. control to default Too low pressure Pressure drops below 35 kPa Check gas supply Pressure fault Pressure state not ready after 5 minutes Check gas supply or replace manifold 490 Micro GC User Manual...
  • Page 82 Flush cycle aborted Flush cycle stopped before completion GC module changed Changing a channel (controller or module) and restarting the instrument TCD Gain calibrated End TCD Gain calibration TCD Offset calibrated End of Offset calibration 490 Micro GC User Manual...
  • Page 83 EDS A.M. Config SVER Electronic Data Sheet Analytical Module incorrect Configuration incorrect EDS A.M. Protected SVER Electronic Data Sheet Analytical Module incorrect Protected Structure Version incorrect Pressure Offset calibration Notification Pressure Offset calibration is complete completed 490 Micro GC User Manual...
  • Page 84 Internal Software Error, ACE reactor stopped. Auto reboot stopped Watchdog Error: Event pump Internal Software Error, Event pump stopped. Auto reboot stopped for 20 s Watchdog Error: IOC Fatal Internal Software Error, IOC fatal error 0 Auto reboot error 0 490 Micro GC User Manual...
  • Page 85 Internal Software Error, IOC fatal error 1 Auto reboot error 1 Watchdog Error: IOC Fatal Internal Software Error, IOC fatal error 2 Auto reboot error 2 Watchdog Error: IOC Fatal Internal Software Error, IOC fatal error 3 Auto reboot error 3 490 Micro GC User Manual...
  • Page 86 Errors 490 Micro GC User Manual...
  • Page 88 INGENIERIA ANALITICA S.L. Tel: (+34) 902.45.6677 Fax: (+34) 902.46.6677 www.ingenieria-analitica.com inf@ingenieria-analitica.com © Agilent Technologies, Inc. Printed in USA, February 2014 G3581-90001...

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