Example: Optimization For Multiple Compounds; Setting The Chromatographic Conditions - Agilent Technologies InfinityLab LC Series User Manual

5
Optimizing the Detector

Example: Optimization for Multiple Compounds

Example: Optimization for Multiple Compounds

Using PNAs as a sample, this example uses the described scanning functions.

Setting the Chromatographic Conditions

This example uses the following chromatographic conditions (the detector
settings are shown in
Table 14
Mobile phases
Column
Sample
Flow rate
Compressibility A (water)
Compressibility B (Acetonitrile)
Stroke A and B
Time Table
Stop time
Post time
Injection volume
Oven temperature (1200)
FLD PMT Gain
FLD Response time
108
Figure 37
Chromatographic Conditions
Agilent InfinityLab LC Series 1260 Infinity II FLD User Manual
on page 109).
A = water = 50 %
B = Acetonitrile = 50 %
Vydac-C18-PNA, 250 mm x 2.1 mm i.d. with 5 µm particles
PAH 0.5 ng
0.4 ml/min
46
115
auto
at 0 min % B=50
at 3 min % B=60
at 14.5 min % B=90
at 22.5 min % B=95
26 min
8 min
1 µl
30 °C
PMT = 15
4 s