Agilent Technologies InfinityLab LC Series User Manual page 100

Fluorescence detectors
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5
Optimizing the Detector
Method Development
Figure 33
The obtained data are combined to setup the time-table for the excitation
wavelength for best limit of detection and selectivity. The optimized switching
events for this example are summarized in
Table 10
Time [min]
0
8.2
19.0
This timetable gives the conditions for optimum detection based on the results
of two chromatographic runs.
100
Optimization of the time-program for the excitation wavelength
Timetable for the analysis of 15 polynuclear aromatic hydrocarbons
Exitation Wavelength [nm]
260
260
260
Agilent InfinityLab LC Series 1260 Infinity II FLD User Manual
Table 10
on page 100.
Emission Wavelength [nm]
350
420
500

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