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GloMax™ 96 Microplate Luminometer
INSTRUCTIONS FOR USE OF PRODUCTS E6501, E6511 AND E6521
Quick Start Guide
Starting a Run
14. Enter any information that you wish to record in the Experiment, Operator,
Plate Number and Notes fields located in the Main Dialog Box.
15. If you are using injectors, you need to prime the injectors before running the
assay.
Place reagent bottles in Reagent Bottle Holder. Secure the inlet tubing in
the reagent bottle by placing the inlet lines under the Tubing Guide on the
left side of the instrument.
Hold the released tips over a waste container during priming.
Click on the Prime tab. Click on "Injector 1", "Injector 2" or "Both Injectors" to
select the injectors. Click "Prime" on the follow-up screen to start pumping.
16. Insert your plate into the sample tray and close the sample tray cover. Close
the instrument lid.
17. Click the "Start" button in the Main Dialog Box to start the run. The Excel
spreadsheet should maximize after the first selected row is measured.
18. After the experiment is finished, you may retrieve any unused reagent by
clicking the Reverse Purge tab.
19. Wash injectors. Place the tip or tips in a waste bottle and click the Flush tab.
Select the injectors to be washed. Wash the injectors three times with water.
Next, wash three times with 70% ethanol, and follow with three more washes
with water. Dry the injectors by flushing them with air three times.
N N o o t t e e : : Remember to remove sample plate after the experiment is finished.
N N o o t t e e : : Do not use bent or damaged injector tips. Check the tips periodically
and replace as needed.
D D o o n n o o t t use bent or damaged injector tips.
For additional information, see the GloMax™ 96 Microplate Luminometer
Technical Manual #TM278, available online at: w w w w w w . . p p r r o o m m e e g g a a . . c c o o m m
ORDERING / TECHNICAL INFORMATION:
www.promega.com • Phone 608-274-4330 or 800-356-9526 • Fax 608-277-2601
© 2006, 2009 Promega Corporation. All Rights Reserved.
Prices and specifications subject to change without prior notice.
Quick
P R O T O C O L
1 2
Injector tip holder
Inlet tubing
Injector
Reagent Bottle
Holder
Waste container
1 2
Injector tip holder
Printed in USA. Revised 3/09.
Part #9FB082
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