Performing A Delay Calibration With An Uv Detector - Agilent Technologies G1364B User Manual

1 Configuration and Operation of the Fraction Collector
Delay volumes and delay calibration

Performing a delay calibration with an UV detector

1 Place a vial containing the Delay Sensor Calibrant (Part No. G1946-85020)
2 Remove the installed column and connect the capillaries with a
3 Connect a bottle of water to Channel A.
4 Install the Delay Calibration Adapter G1364-87301 by attaching it to the
5 Switch to Diagnosis View (if necessary).
6 Open the AFC Delay Volume Calibration status window (see
Figure 4
18
in position 1 of the Autosampler.
zero-dead-volume connector.
needle carrier assembly (G1364B preparative scale only).
the Fraction Collector sub-menu of the Maintenance menu:
Maintenance –> Fraction Collector –> Delay Volume Calibration
AFC Delay Volume Calibration window
The method to be used for the delay volume calibration is displayed
(DGCALAS.M is the default calibration method for the analytical scale
fraction collector and DGCALPS.M is the default method for the preparative
fraction collector); if you want to change to a different method, click the
Change Method button and select a new method. See also
Own Calibration Method"
The Info field shows the current status of the delay volume calibration, and
gives instructions and warnings.
on page 26
Agilent 1100 Series Fraction Collectors User's Guide
Figure 4) from
"Making Your