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Covaris E220 User Manual

E and le-series
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See also: User Manual
For research use only
an automated, batch system for
high performance sample preparation
using adaptive focused acoustic energy
Product Name:
E/LE-series running SonoLab 6.2.4 or later
Product Number:
E220, E220x, LE220, E220R, LE220R
E/LE-series User Manual
E and LE-Series
USER MANUAL
1

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Summary of Contents for Covaris E220

  • Page 1 For research use only E and LE-Series USER MANUAL an automated, batch system for high performance sample preparation using adaptive focused acoustic energy Product Name: E/LE-series running SonoLab 6.2.4 or later Product Number: E220, E220x, LE220, E220R, LE220R E/LE-series User Manual...
  • Page 2 Not for use in diagnostic procedures Publication P/N 010119 Rev J October 2017 Covaris products are covered by Patent No. US 6,719,449, US 6,948,843, and other pending applications Copyright ©2014 Covaris, all rights reserved. Covaris, Inc. 14 Gill Street, Unit H...
  • Page 3 5. Establish a standard of operation and periodically test equipment, as described in the Maintenance section of this manual. 6. Be advised that loading third party software onto the computer may interfere with the performance of the Covaris SonoLab™ software. Please consult Covaris. E/LE-series User Manual...
  • Page 4 Covaris personnel. If Covaris personnel are required to perform on-site repair, all travel costs are paid by the customer. A limited warranty as specified may apply to certain components of the equipment.
  • Page 5 APPENDIX A: SonoLab 7 And 6.2 Software Installation on a Windows XP Or 7 Computer ....26 APPENDIX B: Covaris 220 Series Instrument Driver Update Procedure for Windows 7 ......28 APPENDIX C: Site Preparation and Installation Requirements ................31 APPENDIX D: Humidity and Water Management in Covaris E and LE Systems ........

  • Page 6: Introduction

    Overview of the Manual This manual contains operation and maintenance instructions for the Covaris E and LE-series instruments (the E220, E220x and LE220). It contains background information essential to the proper use and care of this equipment. The following definitions apply in this manual: NOTE: Inconvenience only if disregarded –...

  • Page 7: Principle Of Operation

    Figure 1 Principle of Operation The Covaris Process generates acoustic shock waves to cause controlled cavitation and acoustic streaming at the focal point within the sample treatment vessel. Acoustic energy has been utilized for many years for a variety of diagnostic, therapeutic and research purposes. Well- known uses of sonic or acoustic energy in materials processing include "sonication", an...

  • Page 8: System Distinctions – E, Ex And Le

    • The E220 employs a single focal point transducer to sequentially treat samples as they are positioned over the transducer by the motion system. The E220 may be programmed to a maximum Duty Cycle (acoustic power on/off ratio) of 20.0%.

  • Page 9: Installation

    Ensure that the user-supplied chiller is connected to the CHILLER IN and OUT ports on the left side of the instrument using the Covaris supplied hoses. The hoses are setup with quick disconnect fitting to be attached to the instrument and hose clamps for the user supplied...
  • Page 10 2.4.2 Powering Up the System Power up the Covaris system by moving the power switch to the “1” position. If the computer is supplied by Covaris, turn it on. Open the SonoLab software by double clicking the SonoLab icon on the desktop.

  • Page 11: System Description

    SYSTEM DESCRIPTION General Overview The Covaris E and LE220 systems, as shown in Figure 1, are custom instruments for high- throughput sample preparation and compound management. The system is comprised of the Treatment System which delivers the energy to the sample, the Safety System which provides intelligent interruption during the “treatment”, and the Computer and the Application Software...
  • Page 12 The water level will be dictated by the tube rack. If Covaris has provided an Instruction for Use (IFU) for your tube rack, please refer to it for the recommended water level. If no water level is specified, fill the water bath to the “0”...

  • Page 13: Computer

    approximately 45 minutes to ensure proper operation of the instrument. The degassing pump will start automatically as the transducer is submerged. Its ON status is indicated by a green indicator on the SonoLab main screen. If the degas pump has been turned OFF, it can be restarted by clicking the DEGAS ON button.

  • Page 14: Application Software

    Figure 7 Application Software 3.6.1 Main Panel Software Panel 1: MAIN PANEL (LE220 application shown) Motion Buttons The motion buttons move the sample holder and the transducer. They operate as follows: DEGAS ON starts the degas pump DEGAS OFF turns the degas pump off E/LE-series User Manual...
  • Page 15 LOAD PLATE lowers the transducer from a raised position (SERVICE BATH position) and moves the sample holder to the manual load position. SERVICE BATH raises the transducer and sample holder out of the water bath. METHOD EDITOR button will take the operator to the Method Editor Panel which allows the user to create and/or modify a method.
  • Page 16 ACOUSTIC POWER GRAPH shows the acoustic power being delivered to the sample for each treatment. Plate Map Graphic Each row of wells is represented in the plate map. Untreated wells are yellow. A well undergoing treatment is green and once completed the color changes to blue. If a treatment is aborted, the row will appear red.
  • Page 17 Acoustic energy is produced in bursts and the system allows the user to modify this energy to maximize the effects on the treatment of samples for any particular application. Contact Covaris Technical Support for recommendations regarding these settings. DUTY FACTOR is defined as a percentage. This is the percentage of time that the transducer is “on”...

  • Page 18: System Operation

    Method progress will halt if these values exceed the maxima allowed by each well plate definition. Test dithering functions before using them in on valuable samples. Contact Covaris for guidance if needed. Method Parameters FREQUENCY SWEEPING: The acoustic frequency sweeps through a range during processing.

  • Page 19: Creating And Modifying A Method In The Method Editor Panel

    Degassing The Covaris system requires that the water bath be degassed to maintain repeatable acoustic energy coupling, therefore, the pump should be running whenever samples are being treated.

  • Page 20: Running A Method Manually In The Main Panel

    Running a Method Manually in the Main Panel Load the samples first by selecting the LOAD PLATE button. The motion system will move the sample holder tray to the front of the enclosure and the green DOOR button will illuminate. Press the DOOR button to open the door and position the samples on the tray.

  • Page 21: Shut-Down Procedures

    Dimensions: 23” W x 30” D x 19” H (59cm x 76cm x 48cm) Weight: approximately 110 lbs (50 Kg) Computer Notebook computer (may be supplied by Covaris or the user) Operating System Microsoft Windows XP Professional or Windows 7 (32-Bit Recommended) Application Software Covaris SonoLab 6.2...

  • Page 22: Maintenance

    Test the system periodically. Make sure that the operation is halted when the STOP button is depressed. WARNING: Any failure of the Safety System must be reported immediately to Covaris. DO NOT attempt operation if the Safety System is malfunctioning. E/LE-series User Manual...

  • Page 23: Degassing The System

    Should it be necessary to move the instrument, for whatever reason, the system needs to be properly prepared. This procedure describes the steps necessary to relocate Covaris LE-Series instrument. If the instrument is to be moved off site, a crate may be obtained from Covaris. 6.5.1 Prepare the System •...
  • Page 24 6.5.2 Move of the System • If the instrument is to be moved off-site, procure a crate from Covaris. Pack the instrument in the crate using the provided foam inserts. • Protect the system and computer with a light cloth or plastic to prevent dust contamination.

  • Page 25: Troubleshooting

    TROUBLESHOOTING System Trouble Analysis Chart SYMPTOM PROBABLE CAUSE POSSIBLE SOLUTION Error message “Unknown Device Com ports were Go to C:\Covaris\E-Series Instrument Type” assigned incorrectly v6.2\Program to open the SonoLab configuration file, “SonoLab E-Series.ini”. Replace: ComPortMotion=usb to ComPortMotion=COM4 & ComPortSound=usb to...

  • Page 26: Appendix A: Sonolab 7 And 6.2 Software Installation On A Windows Xp Or 7 Computer

    6. When running SonoLab 7, follow any firmware update instructions that appear. Quit SonoLab 7. Install Covaris SonoLab 6.2.x E and LE-series software on the Windows XP or 7 computer: 7. Begin with the USB cable disconnected and the instrument powered off.
  • Page 27 SonoLab Method Editor. 14. Highlight the plate definitions and copy the files to the following location on the computer: C:\Covaris\SonoLab v6.2\Plates 15. To install an example of a treatment process, open the installer folder and navigate to the Processes folder.

  • Page 28: Appendix B: Covaris 220 Series Instrument Driver Update Procedure For Windows 7

    APPENDIX B: Covaris 220 Series Instrument Driver Update Procedure for Windows 7 Windows Drivers are software files required to support communications between a computer and any USB (Universal Serial Bus) device. Specific drivers are needed to support communications with the E/LE220.
  • Page 29 When the driver software update dialog opens, select the option to ‘Browse my computer for driver software’. g. Point the file open dialog to “c:\Program Files\Covaris\Driver” to find the necessary instrument drivers. E/LE-series User Manual...
  • Page 30 Click next. You will be notified that Windows can’t verify the publisher of this driver software. Click on “Install the driver software anyway”. Windows will install the driver and post the following dialog box. Under Other devices, right click device USB Serial Port and select Update Driver Software.
  • Page 31 Manager under Ports(COM & LPT): • Covaris RF Controller(COM3) • Covaris RF Controller(COM4) • Covaris RF Controller(Port 3&4)(COM5) • Covaris RF Controller(Port 3&4)(COM6) In addition, 4 devices should be added to Device Manager under Universal Serial Bus Controllers: • Covaris RF Controller Board •...

  • Page 32: Appendix C: Site Preparation And Installation Requirements

    APPENDIX C: Site Preparation and Installation Requirements The Covaris E series is delivered in a crate, whose dimensions are approximately 46” wide by 25” deep by 30” high. If doorways or elevators cannot accommodate these dimensions, the instrument and accessories will have to be removed from the crate at the loading dock. If this is necessary, be certain to remove all accessories from the crate before disposal.
  • Page 33 E/LE-series User Manual...

  • Page 34: Appendix D: Humidity And Water Management In Covaris E And Le Systems

    APPENDIX D: Humidity and Water Management in Covaris E and LE Systems Covaris E and LE Systems employ a six-liter tank of distilled or deionized water located inside the instrument housing. This water provides an acoustic path between the system transducer and the vessel in treatment.
  • Page 35 5. Replace the water tank and move instrument to load position 6. Purge the degas pump by turning on for 10 seconds. Purge the WCS lines by connecting the hoses and powering on WCS until flow subsides 7. Turn the WCS off and disconnect hoses. Raise instrument to service position. 8.

  • Page 36: Appendix E: "R" Version Of E220 And Le220 (E220R And Le220R)

    APPENDIX E: “R” version of E220 and LE220 (E220R and LE220R) “R” versions of the E220 and LE220 instruments operate identically to their non-R models, but are equipped with a side-access door and controllable swing arm to allow a robotic system to place a rack or plate of samples on the holder frame of the instrument.

This manual is also suitable for:

E220xLe220rLe220E220r

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