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Product Insert: microTUBE-500 AFA Fiber Screw-Cap
Introduction
The microTUBE-500 AFA Fiber Screw-Cap
sample for sample processing. Each tube contains an AFA fiber that improves reproducibility, promotes an
isothermal process, reduces fragmenting times, and allows larger sample volumes to be processed.
The microTUBE-500s have been validated for DNA shearing at volumes of 320 µl and 500 µl. They are
compatible with the M-series, S220, E-series and L-Series Covaris instruments and require the use of an
instrument specific Rack or Holder. Refer to the Covaris-certified Consumable Guide for further information
(covaris.com/wp-content/uploads/M020065.pdf). DNA shearing protocols with the microTUBE-500 are also
instrument specific. Do not store sheared samples in the microTUBEs.
The microTUBE-500 has a 2D barcode which can be used for sample traceability.
The microTUBE-500s are also a part of the truXTRAC
520252, PN 520262, and PN 520271. Please refer to the Nucleic Acid Extraction from FFPE Protocols:
protocols/nucleic-acid-extraction-from-ffpe/).
Recommended instructions are subject to change without notice.
Ordering Information
• microTUBE-500 AFA Fiber Screw-Cap (25)
• microTUBE-500 Screw-Cap Prep Station
• microTUBE-500 Centrifuge and Heat Block Adapter (10)
DNA Shearing Protocols
• Quick Guide: DNA Shearing with M220 (https://covaris.com/wp-content/uploads/pn_010252.pdf)
• Quick Guide: DNA Shearing with ME220 (https://covaris.com/wp-content/uploads/pn_010349.pdf)
• Quick Guide: DNA Shearing with S220 (https://covaris.com/wp-content/uploads/pn_010368.pdf)
• Quick Guide: DNA Shearing with E220 (www.covaris.com/wp-content/uploads/pn_010308.pdf)
• Quick Guide: DNA Shearing with LE220 (www.covaris.com/wp-content/uploads/pn_010156.pdf)
• Quick Guide: DNA Shearing with LE220-plus/R-plus/Rsc (www.covaris.com/wp-content/uploads/pn_010433.pdf)
Part Number: 010526 Rev A
(PN
520185) is a singular vessel designed to hold up to 500 µl of
FFPE Plus product line and included in the following kits: PN 520255, PN
®
(PN
520186)
(PN
500510)
(PN
500503)
1
Covaris
(covaris.com/resources/
June 2020
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Related Manuals for Covaris microTUBE-500
Summary of Contents for Covaris microTUBE-500
- Page 1 The microTUBE-500s have been validated for DNA shearing at volumes of 320 µl and 500 µl. They are compatible with the M-series, S220, E-series and L-Series Covaris instruments and require the use of an instrument specific Rack or Holder. Refer to the Covaris-certified Consumable Guide for further information (covaris.com/wp-content/uploads/M020065.pdf).
- Page 2 CAUTION: All Covaris microTUBEs must operate within energy constraints. The power maximum levels are guides and should not be exceeded. Each instrument has been tested with the microTUBE-500 for 2x the energy of the 150 bp DNA shearing protocol, and with the truXTRAC FFPE emulsification protocol.
- Page 3 LE220plus_500452 Rack 24 Place microTUBE-500 Screw-Cap +6mm offset LE220Rsc-plus LE220PRSC_500452 Rack 24 Place microTUBE-500 Screw-Cap +6mm offset NOTE: If the plate definition is not present on the system, contact Covaris Technical Support (TechSupport@covaris.com) with the system serial number. Instructions for Use 1.
- Page 4 Covaris Appendix A: Removing or Installing the Intensifier (Covaris PN 500141) from a Covaris E System The 500141 Intensifier is a small inverted stainless steel cone centered over the E-Series transducer by four stainless wires. The wires are held in place by a black plastic ring pressed into the transducer well.
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