1. Manuals
  2. Brands
  3. Covaris Manuals
  4. Laboratory Equipment
  5. E220 evolution
  6. User manual

Covaris E220 evolution User Manual

Focused-ultrasonicator
Hide thumbs Also See for E220 evolution:
Table of Contents

Advertisement

Quick Links

Download this manual
For research use only
Instrument and software developed for
high performance sample preparation
using adaptive focused acoustic energy
Product Name:
E/LE220-Series
Model Numbers:
E220 evolution
Software:
E220 evolution User Manual
evolution
E220
USER MANUAL
SonoLab 7
Page 1

Advertisement

Table of Contents
loading
Need help?

Need help?

Do you have a question about the E220 evolution and is the answer not in the manual?

Questions and answers

Subscribe to Our Youtube Channel

Related Manuals for Covaris E220 evolution

Summary of Contents for Covaris E220 evolution

  • Page 1 For research use only evolution E220 USER MANUAL Instrument and software developed for high performance sample preparation using adaptive focused acoustic energy Product Name: E/LE220-Series Model Numbers: E220 evolution Software: SonoLab 7 E220 evolution User Manual Page 1...
  • Page 2 Registered names and trademarks used in this document, even when not specifically marked as such, are not to be considered unprotected by law. WCS and AFA are either registered trademarks or trademarks of Covaris, Inc. in the United States and/or other countries.
  • Page 3 Establish a standard of operation and periodically test equipment, as described in Maintenance (see section 6.0) of this manual. Loading third party software onto the computer may interfere with system operation. Please consult with Covaris. E220 evolution User Manual Page 3...
  • Page 4 Any component which proves defective during the stated period will be repaired free of charge or replaced at the sole discretion of Covaris, F.O.B., Woburn, Massachusetts, U.S.A. provided the defective component is returned properly packaged with all transportation charges prepaid.

  • Page 5: Table Of Contents

    Cleaning the System ........................37 TROUBLESHOOTING ........................38 Appendix A: Suggested E220 evolution IQ / OQ / PQ ................40 Appendix B: E Series Site or Transport Preparation ................... 46 Appendix C: Installing SonoLab Software, Firmware and Instrument Drivers ..........48 Appendix D: Recirculating Chiller ........................

  • Page 6: Introduction

    This manual contains operation and maintenance instructions for the Covaris E220 evolution Focused-ultrasonicator. This document contains information essential to the proper use and care of the equipment. Should any unforeseen problems occur with the normal operation of the equipment, contact Covaris Technical Support immediately. The following definitions apply in this manual: NOTE: Inconvenience if disregarded.

  • Page 7: Installation And Setup

    Instrument power cord o Computer AC Adaptor power cord • Accessories o Chiller Hose Kit o Sample Tubes & Racks (varies by application) o Water Tank (installed in main unit) E/LE Series Focused-ultrasonicator E220 evolution User Manual Page 7...

  • Page 8: Installation

    Please refer to the rear of the instrument’s right side panel and identify the following features: USB Port Product Safety Label AC Line (Mains) Input and Power Switch Serial Tag, Regulatory and Line Power Label The Rear Right Panel of the System E220 evolution User Manual Page 8...
  • Page 9 CAUTION: To prevent chiller damage, use of a 20% glycol solution in the circulating chiller is required. Please refer to the chiller’s user manual. E220 evolution User Manual Page 9...
  • Page 10 WCS User Manual for further instructions. The WCS removes biological growth and particulates from the water, allowing bath water to remain in place for up to one month. 2.2.13 The instrument is now ready to be started. E220 evolution User Manual Page 10...

  • Page 11: Getting Started

    Start the system computer. The computer employs a Windows Operating System (XP, 7 or 8). SonoLab 7 is an application used to control a Covaris Focused-ultrasonicator for processing a biological or chemical sample. It is designed to create, edit, and run a processing program called a method.
  • Page 12 Following the splash screen, the SonoLab Run Screen will appear with the motion system homing dialog box in front of it: Click ‘Yes’. The transducer and sample holder mechanical assemblies will move into position and the SonoLab ‘Run’ screen will display. E220 evolution User Manual Page 12...

  • Page 13: The Sonolab Run Screen - Running A Method

    If the instrument is powered ON and connected to the laptop via the USB cable, SonoLab will connect to the instrument, display “E220 evolution” at the top center of the screen. If SonoLab does not connect to the instrument, the Run Screen will not appear. SonoLab will display the About Screen and the status “Disconnected”...
  • Page 14 This position may be used to verify that the water level in the bath is correct. • Drip Position raises the sample tray above the bath water to allow excess water to drip from the sample, if desired, prior to removing the sample from the instrument. E220 evolution User Manual Page 14...
  • Page 15 At the same time, the ‘Run’ button becomes a grayed out ‘Abort’ button and the ‘Pause’ button is enabled. See details on pausing the method below. E220 evolution User Manual Page 15...
  • Page 16 The user may, at any time while running a method, click “Pause” to interrupt a running method. When the method is paused, the user may continue the method by clicking the Resume button, or terminate the method by clicking the Abort button. E220 evolution User Manual Page 16...

  • Page 17: System Shutdown

    3.4.2 Close SonoLab Exit SonoLab by clicking on the in the upper right corner. ”Are you sure you want to exit SonoLab?” will appear. “Yes” will close the application. “No” will cancel this request. E220 evolution User Manual Page 17...

  • Page 18: Temperature Control

    Change water daily and rinse with a 10% bleach solution monthly. Temperature Control An important principal of the Covaris Focused-ultrasonicator is isothermal temperature control. The sample is immersed in a water bath which conducts ultrasonic energy while maintaining a stable sample temperature.

  • Page 19: Degas System

    4 of this manual. SonoLab™ APPLICATION SOFTWARE SonoLab is an application used to control the Covaris AFA (Adaptive Focused Acoustics) instrument for processing a sample. It is designed to create, edit, and run a sample processing program called a method. It has provisions to run, pause or stop a method and view execution histories which contain a record of key instrument parameter values during a run.

  • Page 20: Create And Edit Methods

    4.1.1 To edit or create a new method, click the “Edit…” or “New…” button in the “Method” window. The Method Editor window will appear. E220 evolution User Manual Page 20...
  • Page 21 Create Method / Edit Method Screen 4.1.3 Click the ‘Add Step’ button. The Add Method Step window will appear. The Step Types available from the ‘Step Type’ dropdown list are Process, Delay, Prompt and Repeat E220 evolution User Manual Page 21...
  • Page 22 15%, the Average Incident Power will be approximately 15 Watts, but will vary with sample tube type and water level and temperature. In the E220 evolution instrument, the maximum average power is 100 Watts. E220 evolution User Manual Page 22...
  • Page 23 OK or Cancel. Add a Prompt Step By choosing a Repeat step, the user can create a group and a number of repeat iterations. Steps may then be moved into this group (see Available Functions below). E220 evolution User Manual Page 23...
  • Page 24 This action may also be used to move a step into a group. E220 evolution User Manual Page 24...

  • Page 25: Temperature Tab

    The automatic resume attribute can be shut off by un-checking the “Auto resume” box. The Temperature Error Action can be changed to Abort via the pull-down menus. A method which is aborted, however, cannot be resumed. E220 evolution User Manual Page 25...

  • Page 26: Saving Methods In Folders

    Folder dialog will appear. Enter Folder Name, then click OK. The new folder will appear under Methods as shown below. Additional subfolders may be created by highlighting Methods and clicking New Folder, or by highlighting any subfolder and clicking New Folder. E220 evolution User Manual Page 26...

  • Page 27: History Tab

    USB cable. No action by the user is required. The typical connection method will be “USB Port”. The Communications tab allows the user to manually connect with the instrument if automatic connection fails. If connection problems persist, please contact Covaris Service for assistance. E220 evolution User Manual Page 27...
  • Page 28 Log in as “admin”, as shown below. Please note that both Username and Password are case sensitive. Click OK. The Login box will disappear, the Authentication checkbox will be enabled, and the ‘User Administration’ button will become active. Click on ‘User Administratio’n. The User Adminstration Form will appear: E220 evolution User Manual Page 28...
  • Page 29 Authentication tab. To secure the system, make note of the admin password, leave User Authentication enabled, and log out of the admin account by clicking on Log Out (upper right corner of the SonoLab screen: E220 evolution User Manual Page 29...
  • Page 30 Click Save Changes. • Click Yes and log into Task Scheduler Login under your Windows user name. The default Windows user name for computers shipped from Covaris is “Covaris”, with no password. • SonoLab will restart and, if requested, Windows Task Scheduler will delete files at 7:00 pm (19:00) according to the configuration saved under File Settings.
  • Page 31 Click Save Changes. The following message may appear: • Click Yes. The Windows Task Scheduler Login window appears. Log in under your Windows user name. Computers provided by Covaris have a single Windows user, “Covaris”, with no password. • Click OK on “Successfully Save the System Settings”.

  • Page 32: Maintenance Screen

    4.6.1 Firmware Update On occasion, it is necessary for Covaris to make changes to the firmware that operates the instrument. When SonoLab connects to an instrument, it checks the version of firmware that is running on that instrument. If the instrument’s firmware is out of date, SonoLab will alert the operator and suggest that a firmware update should be performed.

  • Page 33: About Tab

    About Tab The About Tab provides the user with basic information about the instrument such as serial number, software version, and board serial numbers. Contact information for Covaris is also displayed. Clicking Help provides detailed Applications and Service information. E220 evolution User Manual...

  • Page 34: Motion Tab

    Clicking on Service Information creates a file, Status.ZIP, which captures status files from the instrument. This can be emailed to Covaris to aid in diagnosing any problems. Motion Tab • The left half side of the Motion screen shows motion parameters and predefined sample tray positions.

  • Page 35: System Specifications

    Microsoft Windows XP SP3, 7 or 8 Application Software: Covaris SonoLab 7 Data Input: Keyboard, mouse Chiller re-circulating system - may be purchased independently or from Chiller: Covaris. Connect with the 3/8 inch I.D. hoses and quick connect fittings supplied. E220 evolution User Manual Page 35...

  • Page 36: Maintenance

    WARNING: Any failure of the Safety System must be reported immediately to Covaris. DO NOT attempt operation if the Safety System is malfunctioning. Degassing System The degassing pump should not be run without a water bath. Extended running without water will cause the pump head to wear out.

  • Page 37: Cleaning The System

    When the pump is running, verify that there are small bubbles coming from the end of the outlet nozzle. If you suspect that the system has become clogged, please contact Covaris for assistance. There is a small inlet tube that can be removed and cleaned.

  • Page 38: Troubleshooting

    TROUBLESHOOTING This section provides a list of symptoms and recommended actions that may be taken to correct simple problems. Please contact Covaris or your local representative for assistance with any problem. • Instrument will not connect to SonoLab (“Disconnected” displayed at top of screen): Verify that the instrument is ON.
  • Page 39 In SonoLab, go to the About tab and click Service Information. This will create a ZIP file on the desktop that can be emailed to Covaris or your local representative. Contact Covaris or your local representative and provide the information listed above.

  • Page 40: Appendix A: Suggested E220 Evolution Iq / Oq / Pq

    Appendix A: Suggested E220 evolution IQ / OQ / PQ All Covaris instruments are rigorously tested at the Manufacturing Facility prior to shipment. This is done to ensure that your Covaris device is of the highest quality and meets your needs for consistent, efficient, and reliable Sample Preparation.
  • Page 41 E220 evolution INSTALLATION QUALIFICATION (IQ) DATE: INSTRUMENT S/N: COMPLETED BY: Action / Requirements Ref. Section Requirement Met? Section 1: Shipping Contents and Instrument Condition Instrument is successfully unpacked and all items noted in Packing List are present. Denote any missing items here: _______________________________ There is no damage evident to the instrument or accessories.
  • Page 42 Verify computer is connected to instrument via USB cable provided. Power on the computer and log in (as required). Note: If Laptop is not provided by Covaris, refer to SonoLab / Driver installation sections of User Manual. Double-click the ‘SonoLab’ shortcut on the computer desktop; verify the application starts and software requests a ‘HOMING’...
  • Page 43 E220 evolution OPERATIONAL QUALIFICATION (OQ) (2 of 2) DATE: INSTRUMENT S/N: COMPLETED BY: Section 3: Degas / Run Method Click ‘Load Position’ button in SonoLab and verify transducer submerges. Click ‘DEGAS PUMP IS OFF’ and ensure degas pump can audibly be heard running and bubbles are exiting the outlet tube.
  • Page 44 E220 evolution PERFORMANCE QUALIFICIATION (PQ) (1 of 2) DATE: INSTRUMENT S/N: COMPLETED BY: Action / Requirements Ref. Section Requirement Met? Power Verification Test *The following can be performed during observation of normal sample processing or as a stand-alone experiment. ...
  • Page 45 E220 evolution PERFORMANCE QUALIFICIATION (PQ) (2 of 2) DATE: INSTRUMENT S/N: COMPLETED BY: Degas Performance Test – OPTIONAL (Calibrated O2 Meter Required)  Fill Water Bath with fresh water (10-15 on ‘Fill’ side of Water Level Label). Use a calibrated Dissolved Oxygen Meter (i.e.

  • Page 46: Appendix B: E Series Site Or Transport Preparation

    A fourth outlet is required if the optional Water Conditioning System (WCS) is installed. The instrument draws a maximum of 500 VA. The Covaris E-Series instruments are configured with a universal power supply. Please note that chillers are generally single-voltage devices and are rated for the country of use.
  • Page 47 E220 evolution User Manual Page 47...

  • Page 48: Appendix C: Installing Sonolab Software, Firmware And Instrument Drivers

    Appendix C: Installing SonoLab Software, Firmware and Instrument Drivers The E220 evolution includes a laptop PC with SonoLab software and device drivers installed. If, however, it is necessary to replace the computer and re-install SonoLab or device drivers, please refer to the following instructions.
  • Page 49 Click on the Windows Start button. Either via Control Panel, or by right-clicking on Computer and choosing Properties, locate Device Manager. Click on this link to open the Device Manager window. In the list of devices, locate Other devices and entries for RF Controller Board: E220 evolution User Manual Page 49...
  • Page 50 Under Other devices, right click first device RF Controller Board and select Update Driver Software: When the driver software update dialog opens, select the option to Browse my computer for driver software: E220 evolution User Manual Page 50...
  • Page 51 Point the file open dialog to “C:\Program Files\Covaris\Driver” and verify that “Include subfolders” is checked: Click next. You may be notified that “Windows can’t verify the publisher of this driver software”. Click on “Install the driver software anyway”: Windows will install the driver and post the following dialog box with a message, “Windows has successfully updated your driver software”.
  • Page 52 Return to Other devices and repeat this process in order (top to bottom), for the 3 remaining entries, until all Covaris device entries have been assigned a COM number under Ports (COM & LPT). The ‘Device Manage’ window should now show: Note that the ‘COM’...

  • Page 53: Appendix D: Recirculating Chiller

    Covaris typically supplies a chiller with each instrument, normally with quick-connect fittings pre-installed. Alternatively, users may purchase a chiller separately. It is recommended that the chiller have a capacity of at least 250 Watts at a set point of 5°C, and able to deliver a minimum pump pressure of at least 14.5 psi...
  • Page 54 Do not use pure antifreeze, as this thickens when cold causing chiller flow faults. The Covaris CH-03 Chiller (PolyScience MM7) has a reservoir capacity of 2.7 liters. Add .5 liters of glycol to the empty reservoir and fill with water to yield approximately a 20% mix.

  • Page 55: Appendix E: Humidity And Water Management In Covaris E And Le Systems

    APPENDIX E: Humidity and Water Management in Covaris E and LE Systems Covaris E and LE Systems employ a six-liter tank of distilled or deionized water located inside the instrument housing. For the system to operate correctly, the water must be filled to an appropriate level (dependent upon the protocol being run), must be clean, free of algae growth, and degassed to retard bubble formation in the acoustic field outside of the sample vessel.
  • Page 56 7. Turn the WCS off and disconnect hoses. Raise instrument to service position. 8. Empty the water tank and dry 9. Gently dry transducer and surfaces of instrument. E220 evolution User Manual Page 56...

Table of Contents