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Covaris ML230 Setup & Instruction Manual

Focused-ultrasonicator, instrument developed for high-performance sample preparation using adaptive focused acoustics (afa)
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Covaris
ML230 Focused-ultrasonicator
Setup & Instruction Manual
Instrument Developed for High-performance
Sample Preparation using Adaptive Focused Acoustics
(AFA
)
®
®
For Research Only
Model Number: ML230 | Software: SonoLab 10.1
ML230 Setup & Instruction Manual
Part Number: 010516 Rev B | March 2020

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Summary of Contents for Covaris ML230

  • Page 1 Setup & Instruction Manual Instrument Developed for High-performance Sample Preparation using Adaptive Focused Acoustics (AFA ® ® For Research Only Model Number: ML230 | Software: SonoLab 10.1 ML230 Setup & Instruction Manual Part Number: 010516 Rev B | March 2020...
  • Page 2 Registered names and trademarks used in this document, even when not specifically marked as such, are not to be considered unprotected by law. WCS and AFA are either registered trademarks or trademarks of Covaris, Inc. in the United States and/or other countries. Teflon is a registered trademark of The DuPont Company.
  • Page 3 • DO NOT employ isopropyl alcohol, ammonia-based or abrasive cleaners on the acoustic assembly. • Sample plates should be stored in a dry place when not in use. • DO NOT load third party hardware, software, or parts onto the system without consulting with Covaris. ML230 Setup & Instruction Manual...
  • Page 4 ML230 Instrument Repair – Warranty repair of the ML230 is provided on site. All labor and expense charges are included. Failure due to accident, abuse, or improper operation is not covered. An Operator’s Manual is provided with the equipment. This manual includes sections on the operating instructions, maintenance guidelines, and troubleshooting tips.

  • Page 5: Table Of Contents

    Appendix A: M-Series Site or Transport Preparation ......................44 Appendix B: Updating SonoLab Software ..........................44 Appendix C: Replacing the ML230 Particle Filter Element (PN 500679) ................45 ML230 Setup & Instruction Manual Part Number: 010516 Rev B | March 2020...

  • Page 6: Introduction

    1.2 The ML230 and the Covaris Process The ML230 Focused-ultrasonicator is based on Covaris Adaptive Focused Acoustics (AFA) technology. This instrument is designed to provide researchers an integrated stand-alone tool to be used in laboratory environment. The instrument is ideal for those who work with DNA shearing and sample preparation.

  • Page 7: Installation And Setup

    - Sample Racks and Consumables, depending on order configuration CAUTION: The ML230 weighs about 80 pounds (36 kg). With the front of the instrument closest to your body, lift the ML230 by gripping from its base plate. Lifting the unit by its plastic case or doors will damage the instrument.
  • Page 8 Figure 2.3 Front view of the ML230 instrument After the instrument is unpacked, position the ML230 on a flat surface near a power outlet. Use both sides of the base to lift the instrument. DO NOT lift by the plastic parts at the front of the instrument.
  • Page 9 • Holding the two quick-connect tubes up and out of the way, slide the empty Waste bottle into the left-most opening at the front of the ML230. The vented cap is toward the rear; the Quick Disconnect cap toward the front of the instrument.
  • Page 10 Figure 2.8 Connecting Water Works Bottle to System 2. Assemble the Sample Tray Assembly to the ML230. • Locate the Sample Tray Assembly in the accessory box Holes for Locating Pins...
  • Page 11 • Slide the Sample Tray Assembly locating pin holes onto the pins on the motion system, behind the safety cover. The sample tray faces to the left. Refer to the image below. Locating Pins Figure 2.11 Mounting Bracket installed in ML230 ML230 Setup & Instruction Manual Part Number: 010516 Rev B | March 2020...

  • Page 12: Levelling The Ml230 Instrument

    Figure 2.13 The power panel of the ML230 instrument 3. As shown in Figure 2.13, plug the AC power cord into the power inlet located about half-way up the left side of the ML230 rear panel. Plug the AC power cord into the power outlet.

  • Page 13: Ml230 Software

    SonoLab 10 has been pre-installed on the ML230 instrument. No further installation or configuration is required. SonoLab is the software used to control a Covaris Adaptive Focused Acoustics (AFA) instrument for processing a sample. It is designed to create, edit, and run a sample processing program called a method. It has provisions to run, pause or stop a method and view execution histories which contain a record of key instrument parameter values during a run.

  • Page 14: Starting The Ml230 Instrument

    Covaris 2.5 Starting the ML230 NOTE: The ML230 is shipped with the sample tray removed. If the sample tray has not already been installed, follow the instructions from Section 2: Installation and Setup. Turn on the ML230 power switch located about half-way up the left side of the rear panel.

  • Page 15: Operation

    Tubes are loaded into appropriate Racks, which are then placed on the ML230 sample tray. An Embedded Processor provides an interface for controlling the ML230 instrument via the touch screen at the front of the ML230. The Electronics Cabinet provides the hardware for controlling the acoustic transducer, the temperature, and sample position during processing.

  • Page 16: Preparing The Ml230 For Use

    There must be an acoustic method selected in the Method box c. The proper rack and consumables must be in place on the ML230 sample tray. See Section 3.5, Loading the Samples d. Water Temperature must be within acceptable Min and Max limits e.
  • Page 17 The default setpoint (in the absence of a selected method) is 20 ºC. d. The ML230 Water Control automatically fills the bath to the appropriate level for the tubes specified in the method when the Run button is touched to start a method.
  • Page 18 Once a method has been loaded and all conditions except temperature and water level are acceptable, the Run button will turn green and the selected acoustic Method can be started. To modify an existing method, see Create and Edit Methods in Section 5. ML230 Setup & Instruction Manual Part Number: 010516 Rev B | March 2020...

  • Page 19: Ml230 Water Control

    The Supply bottle is to the right, next to ML230 sample handling system, and the Waste bottle is to the left of the Supply bottle.
  • Page 20 Figure 3.9 microTUBE loaded in ML230 Sample Rack Open the ML230 safety cover. Place tubes and rack assembly into the ML230 sample tray shown in Figure 3.10. The racks are keyed to load into the sample tray in the correct orientation only.

  • Page 21: Running A Method

    By default, the light will be lit once the instrument is turned on. This light may be turned off by touching the Light control on the Home screen. ML230 Setup & Instruction Manual Part Number: 010516 Rev B | March 2020...

  • Page 22: User Maintenance

    • Touch “Stop”, then touch “Empty” in the Water Control panel and allow the bath to drain. • Empty and thoroughly rinse the Supply bottle, fill the Supply bottle with deionized water, and reinstall bottle to the ML230 bottle bay.

  • Page 23: Shutdown Procedure

    CAUTION: Leaving water in the water bath for an extended time may allow algae growth which will interfere with sample processing. Turn off the ML230 by moving the power switch to the OFF position. The power switch of the ML230 is located on the rear panel of the ML230, about half-way up the left side, above the power cord.

  • Page 24: Using A Pre-Installed Method

    4. Using a Pre-installed Method SonoLab has protocols pre-installed on the ML230. These methods have been developed by Covaris scientists and are ready-to-use for some common applications. To select a pre-installed protocol, touch the Method field to display the list of available methods.

  • Page 25: Create And Edit Methods

    Figure 5.1 New Method Button on Main Screen The Method Editor window will appear (See Figure 5.2). Plate Definition Drop-down List Add Process Figure 5.2 Method Editor Window ML230 Setup & Instruction Manual Part Number: 010516 Rev B | March 2020...
  • Page 26 Touch “Process”. The process bar is displayed. If steps are to be repeated, refer to the Repeat Function listed in Section 5.1.5. Touch the + button inside the process bar to display the step type menu. ML230 Setup & Instruction Manual Part Number: 010516 Rev B | March 2020...
  • Page 27 For a given Peak Incident Power, a larger Duty Factor results in more acoustic energy generated from the transducer. Users can define the Duty Factor from 1 to 50% in the ML230 instrument, depending on the PIP chosen.
  • Page 28 SonoLab displays the Average Incident Power, calculated from the Peak Incident Power and Duty Factor, adjacent to the treatment parameters in the treatment step bar. In the ML230 instrument, the maximum Average Incident Power is 100 Watts (for example, 400 watts Peak Incident Power and 25% Duty Factor are selected). SonoLab does not allow the user to select a combination of Peak Incident Power and Duty Factor that results in more than 100 Watts average incident power.
  • Page 29 When the prompt step is reached during the treatment, the method pauses, and the prompt message will be displayed as shown in Figure 5.8. Figure 5.8 Example of a Prompt Message Display ML230 Setup & Instruction Manual Part Number: 010516 Rev B | March 2020...

  • Page 30: Modify Existing Method

    To modify an existing method, select the method as described previously, then touch the “Edit” button. The method editor window will be displayed. Touch to Display Available Methods ML230 Setup & Instruction Manual Part Number: 010516 Rev B | March 2020...
  • Page 31 Select a method to edit here then touch Edit. Figure 5.10 Selecting a Method to Edit Make the desired changes to the method, then save as described above. ML230 Setup & Instruction Manual Part Number: 010516 Rev B | March 2020...

  • Page 32: Sonolab Application Software

    Figure 6.1 The Home Screen Touch the ‘System Status’ button to display the ‘System Status’ pane. The ‘System Status’ pane, as explained in Section 3, displays the states of various ML230 subsystems, indicating whether the ML230 is ready to operate.
  • Page 33 • Stop: stops any current motion. Touch the ‘Water Control’ button to display the ‘Water Control’ pane. The ‘Water Control’ pane displays the current temperature, level, and degassing states of the ML230 water bath. The pane also contains the following buttons: •...

  • Page 34: Schedule Tab

    UV fixture, preserving water cleanliness. The selected method is set to none. The operator must be logged in as Administrator, or higher permission level, to adjust the parameters on this screen. ML230 Setup & Instruction Manual Part Number: 010516 Rev B | March 2020...
  • Page 35 - Touch the Method drop-down list and select the method to be run. - Water level will be set at the beginning of each method run. But, if the ML230 may be idle for long periods of time, click the Enable AutoFill on/off switch so that On is highlighted in dark blue. SonoLab will automatically maintain the correct water level, maintain the degassing, and maintain temperature.

  • Page 36: History Tab

    NOTE: If a method is running at the time the scheduler is set to switch the ML230 to the OFF state, SonoLab ignores the scheduler and the ML230 will not be switched to standby on that day.

  • Page 37: About Tab

    The About tab provides user basic information about the instrument such as serial number, software version, board serial numbers etc. See Figure 6.6. Figure 6.5 About Tab ML230 Setup & Instruction Manual Part Number: 010516 Rev B | March 2020...

  • Page 38: Admin Tab

    • Touch the ADMIN tab. The list of default SonoLab users, their roles, and assigned methods is displayed, along with the “USERS” and “GENERAL” tabs at the left side. ML230 Setup & Instruction Manual Part Number: 010516 Rev B | March 2020...
  • Page 39 • Log into SonoLab as Administrator and touch the ADMIN tab. • Touch the NEW button at the upper right side of the screen. The New User pane is displayed. ML230 Setup & Instruction Manual Part Number: 010516 Rev B | March 2020...
  • Page 40 • Enter a new Username, the user’s first and last names, and touch the “Assigned” button. By default, all treatment methods on the system will be assigned to the new user. ML230 Setup & Instruction Manual Part Number: 010516 Rev B | March 2020...
  • Page 41 Once a new password is entered, it must be entered in again to verify its correct spelling. Touching “OK” returns the user to the login screen where the user may now enter their new password and log in. ML230 Setup & Instruction Manual Part Number: 010516 Rev B | March 2020...

  • Page 42: System Specifications

    Microsoft Windows 10 Application Software Covaris SonoLab Data Input Touch Screen Chiller Solid state chiller for heating and cooling (built-in) Power 0 to 160 Watts, 24 volts ML230 Setup & Instruction Manual Part Number: 010516 Rev B | March 2020...

  • Page 43: Troubleshooting

    Water in bath will not completely drain Use the Fill button to fill the bath above level 1 then use the Empty button to start the drain operation. - Verify the ML230 is level (see Section 2) Shearing not as expected or inconsistent...

  • Page 44: Appendix

    Water Requirements The water bath should be drained when the ML230 is not in use for more than a few days. In normal operation, water is automatically added to the bath as necessary by the Water Control system. Sensors notify the user if the Water Works supply bottle is empty or if the waste bottle is full.

  • Page 45: Appendix C: Replacing The Ml230 Particle Filter Element (Pn 500679)

    Thumb latches Figure C1 Quick Disconnect Components 2. Remove the Supply and Waste bottles by sliding them forward and clear of the ML230. 3. Locate the filter in a recess at the far-left side of the cabinet, inside the bottle bay.
  • Page 46 Press the release tab of the lower fitting and separate bottom of filter from the water line. Press the release tab at the upper end of the filter, and remove the filter from the ML230. Press the release tab at the upper end of the filter, and remove the filter from the ML230.

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