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Covaris LE220 Setup & Instruction Manual

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Covaris
LE220 (-plus, R-plus, and Rsc)
Setup & Instruction Manual
Instrument and Software Developed for High-performance Sample
Preparation using Adaptive Focused Acoustic
(AFA
) Energy
®
®
For Research Only
Model Numbers: LE220-plus, LE220R-plus, and LE220Rsc | Software: SonoLab 8
LE220 (-plus, R-plus, and Rsc) Setup & Instruction Manual
Part Number: 010398 Rev H | November 2019

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Summary of Contents for Covaris LE220

  • Page 1 Preparation using Adaptive Focused Acoustic (AFA ) Energy ® ® For Research Only Model Numbers: LE220-plus, LE220R-plus, and LE220Rsc | Software: SonoLab 8 LE220 (-plus, R-plus, and Rsc) Setup & Instruction Manual Part Number: 010398 Rev H | November 2019...
  • Page 2 Registered names and trademarks used in this document, even when not specifically marked as such, are not to be considered unprotected by law. WCS and AFA are either registered trademarks or trademarks of Covaris, Inc. in the United States and/or other countries. Teflon is a registered trademark of The DuPont Company.
  • Page 3 • DO NOT run an acoustic treatment with the sample cover in the OPEN position or without a water bath - the acoustic system will not work. • If there is any indication that the Safety System is not functioning properly, DO NOT operate the equipment and contact Covaris immediately.
  • Page 4 The customer is expected to perform basic diagnostics and component replacement via telephone support from Covaris personnel. If Covaris personnel are required to perform on-site repair, all travel related costs are paid by the customer. A limited warranty as specified may apply to certain components of the equipment.

  • Page 5: Table Of Contents

    Appendix A: LE220R-plus (LE220-plus) Series Site or Transport Preparation ................48 Appendix B: Installing SonoLab 8 and Required Support Software .................... 49 Appendix C: Humidity and Water Management for the Covaris LE220-plus ................54 Appendix D: Connecting AutoFill to Plant Water ........................56 Addendum for LE220Rsc ..................................

  • Page 6: Introduction

    1.3 The LE220R-plus Instrument In addition to the features of the LE220-plus described above, the LE220R-plus is designed to be integrated with a sample handling robot in an automated work cell. The front door of the safety enclosure contains a cutout providing the robot arm access to the inside of the LE220R-plus cabinet.

  • Page 7: Installation And Setup

    CAUTION: When positioning the system, please keep the fan and rear panel vents free of obstructions that may block the flow of air. LE220 (-plus, R-plus, and Rsc) Setup & Instruction Manual Part Number: 010398 Rev H | November 2019...
  • Page 8 Connect the hoses between the WCS and the CH05 as shown above. Connect the remaining two hoses between the two ports on your LE220R-plus or LE220-plus water bath and the remaining two ports on the opposite side of the WCS. It doesn’t matter which bath port connects to which WCS port for these two hoses.
  • Page 9 2.2.2 The AutoFill option allows plant deionized water to be plumbed directly to the LE220R-plus and LE220-plus to allow automatic filling of the water bath without any operator intervention. If the AutoFill option is going to be used, refer to the instructions included with the AutoFill accessory kit or Appendix D of this manual to assemble the components of the AutoFill Accessory Kit, and connect plant water to the system.
  • Page 10 Remove and discard the desiccant pack. The instrument is now ready to be started. Safety Enclosure Water Tank LE220 (-plus, R-plus, and Rsc) Setup & Instruction Manual Part Number: 010398 Rev H | November 2019...

  • Page 11: Getting Started

    SonoLab is installed on the computer purchased with the instrument. No further installation or configuration is required. Find the SonoLab icon on the desktop: If the icon is not visible, or if SonoLab is not installed, please refer to Appendix B or contact the Covaris Technical Support for installation instructions.
  • Page 12 Following login, the motion system homing dialog appears: Click ‘Yes’. The transducer and sample holder mechanical assemblies will move into position and the SonoLab Home Screen will display. LE220 (-plus, R-plus, and Rsc) Setup & Instruction Manual Part Number: 010398 Rev H | November 2019...

  • Page 13: Preparing Your System For Use

    The safety enclosure door must be closed. NOTE: The proper plate and consumables must be in place on your system’s sample tray. LE220 (-plus, R-plus, and Rsc) Setup & Instruction Manual Part Number: 010398 Rev H | November 2019...
  • Page 14 The method’s run time (minutes and seconds) and temperature set point will be displayed in the lower right corner of the main screen as shown. LE220 (-plus, R-plus, and Rsc) Setup & Instruction Manual Part Number: 010398 Rev H | November 2019...
  • Page 15 Covaris c) An important principal of the Covaris Focused-ultrasonicator is isothermal temperature control. Your system automatically adjusts the water temperature, as necessary, to match the method temperature set point once a method is selected. Water Temperature status will turn green when temperature is within the Min and Max limits (by default 5 degrees above or below the set point). The default set point (in the absence of a selected method) is 20 ºC.
  • Page 16 Once a method has been loaded and all conditions are acceptable, the ‘Run’ button will turn green and the selected acoustic Method can be started. To modify an existing method, see Section 5 Create and Edit Methods. LE220 (-plus, R-plus, and Rsc) Setup & Instruction Manual Part Number: 010398 Rev H | November 2019...
  • Page 17 After the last row has been scanned, the entry window closes, and the yellow “Scan” button becomes a green “Run” button, as described above. LE220 (-plus, R-plus, and Rsc) Setup & Instruction Manual Part Number: 010398 Rev H | November 2019...
  • Page 18 Covaris • RFID: Beginning in SonoLab version 8.4, RFID scanning is implemented. When a method employing the Covaris AFA-TUBE is started, ® the sample tray is passed near an RFID reader mounted to the back of the water bath. An encrypted chip embedded in the AFA- TUBES’s barcode label is read.
  • Page 19 - Double click anywhere in table shown in the image above to display the Automations Settings window. Note: To configure a schedule for just one specific day, Sunday through Monday, double-click on that day in the table. LE220 (-plus, R-plus, and Rsc) Setup & Instruction Manual Part Number: 010398 Rev H | November 2019...
  • Page 20 - Click on either AM or PM, then on the hour the degas pump is to start. The clock changes to display the hour selected (7 o’clock in this example) and the minutes part of the time highlighted. LE220 (-plus, R-plus, and Rsc) Setup & Instruction Manual Part Number: 010398 Rev H | November 2019...
  • Page 21 Note: If your system is left in Standby mode, i.e., the transducer is up and out of the water bath, automatically scheduled events will not occur. LE220 (-plus, R-plus, and Rsc) Setup & Instruction Manual Part Number: 010398 Rev H | November 2019...

  • Page 22: Running A Method

    As the method runs, the graphic for the row under treatment flashes green, and the actual average incident power and temperature data are displayed, as shown. LE220 (-plus, R-plus, and Rsc) Setup & Instruction Manual Part Number: 010398 Rev H | November 2019...

  • Page 23: Daily System Shutdown

    Leaving water in the water bath for an extended time without the WCS operating may promote algal growth in the tank and lines, this growth can interfere with water quality and sample processing. LE220 (-plus, R-plus, and Rsc) Setup & Instruction Manual Part Number: 010398 Rev H | November 2019...

  • Page 24: Create And Edit Methods

    1) Enter the name of the new method in “Name” box as shown. 2) Select the appropriate plate for the consumables used in this method from the “Plate Definition” drop-down list. LE220 (-plus, R-plus, and Rsc) Setup & Instruction Manual Part Number: 010398 Rev H | November 2019...
  • Page 25 4.1.2 Choosing a Treatment Choosing Treatment allows editing of the four acoustic parameters: Duration, Peak Incident Power, Duty Factor, and Cycles per Burst. LE220 (-plus, R-plus, and Rsc) Setup & Instruction Manual Part Number: 010398 Rev H | November 2019...
  • Page 26 SonoLab displays the Average Incident Power, calculated from the Peak Incident Power and Duty Factor, adjacent to the treatment parameters in the treatment step bar. In the LE220- plus, the maximum Average Incident Power is 250 Watts (500 watts Peak Incident Power and 50% Duty Factor).
  • Page 27 Covaris - Dithering: The LE220 motion system provides dithering motion capability in the X axis (fore-&-aft), Y axis (left-to-right), and Z axis (up- and-down). If a treatment specifies dithering, click on the “Pen-&-Paper” icon on the right end of the Process bar...
  • Page 28 As an example, choosing a Delay step introduces a fixed time delay into a method which allows the sample to settle between two different treatment steps. The delay is specified in seconds. LE220 (-plus, R-plus, and Rsc) Setup & Instruction Manual Part Number: 010398 Rev H | November 2019...
  • Page 29 Add a Prompt Step: When the prompt step is reached during the treatment, the method pauses, and the prompt message will be displayed as shown in Figure 4.9. LE220 (-plus, R-plus, and Rsc) Setup & Instruction Manual Part Number: 010398 Rev H | November 2019...

  • Page 30: Creating Methods Using Repeat Function

    Scenario A is used if each row requires one or more acoustic doses, but there is no need for resting the sample between doses. 4.2.1 Implement Scenario A: - Create a method with a Process step, as explained in section 4.1.1 above. LE220 (-plus, R-plus, and Rsc) Setup & Instruction Manual Part Number: 010398 Rev H | November 2019...
  • Page 31 Scenario B is typically used in “pulsing”. That is, a row is treated with an acoustic dose, and is then allowed to rest while the remaining rows are treated. Once the remaining rows are treated, a second acoustic dose is applied to each row. LE220 (-plus, R-plus, and Rsc) Setup & Instruction Manual Part Number: 010398 Rev H | November 2019...
  • Page 32 - Click the + button inside the Process bar and create one or more treatment steps. Two treatment steps are created in this example. LE220 (-plus, R-plus, and Rsc) Setup & Instruction Manual Part Number: 010398 Rev H | November 2019...

  • Page 33: Scanning Treatments (Le220Rsc Only)

    - Click the “add process” button again. - Select Scan from the Step Type list. - Enter the desired treatment parameters as for a normal treatment step. LE220 (-plus, R-plus, and Rsc) Setup & Instruction Manual Part Number: 010398 Rev H | November 2019...

  • Page 34: Modifying Existing Methods

    Figure 4.10 Selecting a Method to Edit - Click Save and give the method a name as with normal treatment methods. Contact our Covaris Applications Support Department for assistance in specifying the appropriate scanning speed and treatment parameters for your application.

  • Page 35: Sonolab Application Software

    (complete, aborted, failed). Figure 5.1 The History Tab LE220 (-plus, R-plus, and Rsc) Setup & Instruction Manual Part Number: 010398 Rev H | November 2019...
  • Page 36 See Figure 5.3. Figure 5.3 Additional History Details LE220 (-plus, R-plus, and Rsc) Setup & Instruction Manual Part Number: 010398 Rev H | November 2019...

  • Page 37: About Tab

    If this occurs, click the “OK” button to continue to the “History” screen. However, the error will be presented each time the “History” tab is selected, until the offending file is removed. To remove the corrupt file, navigate to Documents\Covaris\SonoLab\History, then locate the offending file, in this example, “fddf_04_14_2016-09-10-38.xhst”. Drag this file to the Recycle Bin.

  • Page 38: Admin Tab

    • Can run methods assigned to them by an administrator. • Can review the history screens for methods run by this operator. Managing Users Figure 5.6 Admin Tab LE220 (-plus, R-plus, and Rsc) Setup & Instruction Manual Part Number: 010398 Rev H | November 2019...
  • Page 39 A user can only run methods in their “Assigned Methods” list, or methods they create. 5.4.3 Click the ‘Save’ button to save changes, the ‘Cancel’ button to exit without changes. LE220 (-plus, R-plus, and Rsc) Setup & Instruction Manual Part Number: 010398 Rev H | November 2019...
  • Page 40 Clicking the ‘Add’ button displays the ‘Add/Edit User’ window. Figure 5.10 Add User Screen • Select the Role from the drop-down list. Figure 5.11 Select User’s Role LE220 (-plus, R-plus, and Rsc) Setup & Instruction Manual Part Number: 010398 Rev H | November 2019...
  • Page 41 The user enters their new password, and then must enter it again to verify correct spelling. Clicking “OK” returns the user to the login screen, where the user may now enter the new password and log in. LE220 (-plus, R-plus, and Rsc) Setup & Instruction Manual Part Number: 010398 Rev H | November 2019...

  • Page 42: System Specifications

    Can be programmed from +0.5 °C to +55.0 °C Computer Notebook computer Operating System Microsoft Windows 10 Application Software Covaris SonoLab Data Input Keyboard, mouse LE220 (-plus, R-plus, and Rsc) Setup & Instruction Manual Part Number: 010398 Rev H | November 2019...

  • Page 43: Maintenance

    10) Empty the tank, rinse, and fill with fresh DI water. 11) Place the water tank back into the LE220. 12) Click ‘Home’. The transducer lowers into the bath and the degas pump starts. Allow pump to operate for 3 or 4 minutes.

  • Page 44: Transducer

    Test the system periodically. Verify that the acoustic treatments are disabled when the ‘Stop’ button is pressed, or if the water level in the tank is too low. Verify that the door cannot be opened during an acoustic treatment. WARNING: Any failure of the Safety System must be reported immediately to Covaris. DO NOT attempt operation if the Safety System is malfunctioning.
  • Page 45 CAUTION: Leaving water in the water bath or degassing lines for an extended time may promote algal growth in the tank and lines, this growth can interfere with water quality and sample processing. LE220 (-plus, R-plus, and Rsc) Setup & Instruction Manual Part Number: 010398 Rev H | November 2019...

  • Page 46: Troubleshooting

    Covaris 8. Troubleshooting This section provides a list of symptoms and recommended actions that may be taken to correct simple problems. Please contact the Covaris Technical Support Dept. or your local representative for assistance with any issues. • If your instrument will not connect to SonoLab: - Verify that the instrument is ON.
  • Page 47 ○ Lower the PIP (Peak Incident Power) – this is often more effective than applying very high power. Repeat the method to verify that overload problems have been mitigated. If not, please contact Covaris or your local representative. Technical Support – Ongoing assistance with the operation or application of the equipment and/or troubleshooting is provided via: •...

  • Page 48: Appendix

    Power Requirements Provide at least four standard outlets, one for the laptop computer, one for the LE220-plus, one for the CH05, and one for the WCS 2.0. Your system draws a maximum of 12.5A. All components are configured with universal power supplies. Note: to avoid derating the CH05 cooling capacity, the CH05 may be used with a step-up transformer if operated at less than 115 VAC.

  • Page 49: Appendix B: Installing Sonolab 8 And Required Support Software

    SonoLab 8 using the Windows Uninstall Program control panel. Then install the new version by double-clicking its “setup” file. 1. Install 210063 SQL Server Express, Covaris Part Number 210063 Double-click the “SQLServer2016-SSEI-Expr.exe” file to start the installation. Confirm you wish to install, if prompted. The Express Edition window will display.
  • Page 50 Copy entire 210079 folder to desktop, open and double-click the “BalluffVCPInstaller_x64.exe” file to run the installation. 5. Install 210062 Plate Installer Copy the contents of the 210062 Plate Definition Files folder “Plates_210062_revX” to: C:\Users\Public\PublicDocuments\Covaris\SonoLab\Plates LE220 (-plus, R-plus, and Rsc) Setup & Instruction Manual Part Number: 010398 Rev H | November 2019...
  • Page 51 Manager. Click on this link to open the Device Manager window. In the list of devices, locate Other devices and entries for RF Controller Board: Under Other devices, right click first device RF Controller Board and select Update Driver Software: LE220 (-plus, R-plus, and Rsc) Setup & Instruction Manual Part Number: 010398 Rev H | November 2019...
  • Page 52 When the driver software update dialog opens, select the option to Browse my computer for driver software: Point the file open dialog to “C:\Program Files(x86)\Covaris\Driver” and verify that “Include subfolders” is checked: Windows will install the driver and post the following dialog box with a message, “Windows has successfully updated your driver software”.
  • Page 53 ‘USB Serial Port’ and select ‘Update Driver Software…’, as shown in the image below. Repeat the installation steps shown above. Return to Other devices and repeat this process in order (top to bottom), for the 3 remaining entries, until all Covaris device entries have been assigned a COM number under Ports (COM &...

  • Page 54: Appendix C: Humidity And Water Management For The Covaris Le220-Plus

    Appendix C: Humidity and Water Management for the Covaris LE220-plus Your Covaris instrument employs a six-liter tank of distilled or deionized water located inside the instrument housing. For the system to operate correctly, the water must be filled to an appropriate level, clean, free of algae growth, and degassed to retard bubble formation in the acoustic field outside of the sample vessel.
  • Page 55 Covaris Light Curtain Mounted to the LE220R-plus Robot Arm Accessing LE220R-plus Sample Tray LE220 (-plus, R-plus, and Rsc) Setup & Instruction Manual Part Number: 010398 Rev H | November 2019...

  • Page 56: Appendix D: Connecting Autofill To Plant Water

    30 psi water pressure. Verify that there are no leaks. CAUTION: All AutoFill components have pressure ratings above Covaris’ specified maximum operating pressure and have been tested extensively. However, a catastrophic failure would result in substantial plant water spillage. It is the customer’s responsibility to specify and implement appropriate containment/alarm schema for their facility.

  • Page 57: Addendum For Le220Rsc

    Addendum for LE220Rsc Covaris developed the LE220Rsc to allow scanning treatments of 96 and 384 well plates. This requires additional travel front-to-back (X axis) of which the LE220R-plus is not capable. To accomplish the extra range of motion, the LE220Rsc employs an X axis motion stage with an additional inch of travel.

This manual is also suitable for:

Le220 plusLe220 r-plusLe220 rsc

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