Covaris M220 User Manual
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Covaris M220 User Manual

M-series
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For research use only
instrument developed for
high performance sample preparation
using adaptive focused acoustics
Product Name:
Model Numbers:
Software:
M -Series
USER MANUAL
M-Series
M220
SonoLab 7

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Summary of Contents for Covaris M220

  • Page 1 For research use only M -Series USER MANUAL instrument developed for high performance sample preparation using adaptive focused acoustics Product Name: M-Series Model Numbers: M220 Software: SonoLab 7...
  • Page 2 International Standards Organization (ISO) 15190:2003, Medical Laboratories – Requirements for Safety Trademarks Patented or patent pending and registered or registration-pending are trademarks of Covaris. Registered names and trademarks used in this document, even when not specifically marked as such, are not to be considered unprotected by law.
  • Page 3 The Tube Holder should be stored in a dry place or in the M220 Accessories Storage Station when not in use. Establish a standard of operation and periodically test equipment, as described in Appendix A of this manual.
  • Page 4 Any component which proves defective during the stated period will be repaired free of charge or replaced at the sole discretion of Covaris, F.O.B., Woburn, Massachusetts, U.S.A. provided the defective component is returned properly packaged with all transportation charges prepaid.

  • Page 5: Table Of Contents

    TABLE OF CONTENTS INTRODUCTION ............................ 7 Overview of the Manual ......................7 The M220 and the Covaris Process ..................7 INSTALLATION AND SETUP ....................... 7 Assembly ........................... 7 Installing the Tube Holder ......................9 SonoLab Installation ....................... 10 Starting SonoLab ........................11 OPERATION ............................
  • Page 6 Intentionally blank M220 User Manual Page 6...

  • Page 7: Introduction

    INSTALLATION AND SETUP Assembly The following items should be found in the package of the Covaris M220 instrument: Quick Start User Guide User Manual Notebook Computer with AC Adapter (# 500311) Cables ...
  • Page 8 AFA-grade water (125ml) M220 Safety Cover M220 Main Unit CAUTION: Lift the M220 out of the box by its baseplate. Do not lift by the Acoustic Assembly. Lifting the unit by its acoustic assembly may damage the instrument. Computer Loading...

  • Page 9: Installing The Tube Holder

    Figure 2.2. Gently press the computer to secure it in place. Center the computer on top of the M220 as shown Figure 2.2 - Secure the computer on top of the M220 as shown. USB port To computer Power Switch Ventilation Power Inlet Figure 2.3 - The back panel of the M220 instrument...

  • Page 10: Sonolab Installation

    Figure 2.4 - Place the Tube Holder in the Acoustic Assembly SonoLab Installation SonoLab 7 is an application used to control a Covaris AFA (Adaptive Focused Acoustics) instrument for processing a sample. It is designed to create, edit, and run a sample processing program called a method.

  • Page 11: Starting Sonolab

    Starting SonoLab Turn on the computer. Turn on the power switch located at the back of the M220 instrument. Wait at least 10 seconds to allow the instrument to initialize. Double click the SonoLab 7 icon on the desktop to start SonoLab. An animated splash screen will be displayed, followed by the SonoLab 7 Run screen, as illustrated in Figure 2.5.

  • Page 12: Operation

    The Tube Holder is designed to hold the sample tube at the appropriate position in the acoustic field during processing. It is normally inserted into the top of the Acoustic Assembly. The Computer provides an interface and software to the operator for controlling the M220 instrument through SonoLab 7.

  • Page 13: Preparing The M220 For Use

    Preparing the M220 for Use To start an acoustic treatment, four conditions must be met: • There must be an acoustic method selected in the Method box • Water Temperature must be within acceptable Min and Max limits • Water Level must be at or above that required for correct operation •...
  • Page 14 To achieve an acceptable status on these conditions: • The M220 system automatically adjusts the water temperature to match the set point, and Water Temperature status will turn green when temperature is within the Min and Max limits (typically 2 degrees above and below the set point). The default set point (in the absence of a selected method) is 20ºC.

  • Page 15: Filling The Water Bath

    To transmit acoustic energy to the sample vessel, the Acoustic Assembly must be filled with AFA- grade water. Covaris recommends AFA-grade Water, PN 520101. You can also use highly purified water (at least ASTM Type III or ISO grade 3). To fill the water bath, follow the steps listed below:...

  • Page 16: Loading The Sample

    As shown in Figure 3.7, place the Sliding Weight on the top of the sample tube by rotating the (step 1) and lowering (step 2) Sliding Weight. The Sliding Weight will be maintained at running position. Close the Safety Cover to ensure that the Door status in SonoLab turns to a green check. M220 User Manual Page 16...

  • Page 17: Running A Method

    Use the disposable cleaning swab to sweep the water channels in the Acoustic Assembly. Wipe the surfaces of the transducer and the metal housing with lint-free cloth and air dry for next use. Store the Tube Holder in a dry place or in the M220 Accessories Storage Station (PN 500352); close the Safety Cover.

  • Page 18: Using A Preexisting Method

    SonoLab has protocols preinstalled on the computer running SonoLab 7. These methods have been developed by Covaris scientists and are ready to use for most common applications. To select a preloaded protocol, click on the pull down button to make the list appear.

  • Page 19: Create And Edit Methods

    Figure 4.2 - Method Editor Window The Add Method Step window appears as shown in Figure 4.3. The Step Types available in this screen are Treatment, Delay, Prompt and Repeat 5.1.1 Choosing Treatment allows editing of the four acoustic parameters: M220 User Manual Page 19...
  • Page 20 This parameter indicates the number of electrical signals used in each acoustic “burst of” the transducer. One may define Cycles per Burst from 50 to 1000 in the M220 for different applications. Advanced users may use this parameter to fine tune their experiments.
  • Page 21 By choosing a Repeat step, the user can choose how many iterations they would like to apply to a method. See Figure 4.6. Figure 4.6 Add a Repeat Step 5.1.5 When all method parameters and steps have been defined, click on ok to save changes. M220 User Manual Page 21...
  • Page 22 If the selected step is in a group, a confirmation window will appear asking if the user wants to move the step out of the group. Select All Allows the user to select all steps created. M220 User Manual Page 22...
  • Page 23 To edit an existing method, pull down the name of an existing method (see Figure 4.1) and click “Edit…” 5.2.1 Methods may be Edited in a similar manner as creating a method if the user has the required Authorization (see User Authentication.) M220 User Manual Page 23...
  • Page 24 18.5, once temperature rises to this value the unit will automatically run. 5.4.2 User has the option to choose whether pause or abort method when Maximum or minimum Temperature error occurs. Auto resume Pause/Abort Method M220 User Manual Page 24...

  • Page 25: Sonolab™ Application Software

    Temp Graph Figure 5.2 - Example of History Tab CAUTION: The history list can become long and slow the system. By default, log files older than 120 days are automatically deleted. (See Section 6.3.8 for details). M220 User Manual Page 25...

  • Page 26: Setup Screen

    A user can select “Simulation” mode (See Figure 5.3) to run SonoLab 7 without connecting to an instrument. In the Run tab, user can create and edit methods. Click on the “Simulate” button to run a simulation of the method. Figure 5.4 – Simulation Mode M220 User Manual Page 26...
  • Page 27 Figure 5.5 - The User Authentication Tab By default, authentication is disabled. In order to enable user authentication, a log in id and password is required. Please contact the Covaris Customer Service to obtain Administrator user name and password. The SonoLab authentication model supports 3 types of users: •...
  • Page 28 Click the “User Administration” button (See Figure 5.5) The User Administration window will appear. (See Figure 5.6) • Click the “Add New User…” button. Add New User Edit User Remove User Save Figure 5.6 User Administration Screen M220 User Manual Page 28...
  • Page 29 Select the user that needs to be edited. • Click “Edit User(s)” button. • Administrator can edit user password and role. • Click OK. • Click Save in the User Administration dialog. Figure 5.8 – Edit User Dialog M220 User Manual Page 29...
  • Page 30 Click on “Automatically delete…”check box to turn on auto delete. • Enter or move the up/down arrow to specify how old the log files must be to be deleted. • Click Save Changes. Figure 5.9 –File Settings M220 User Manual Page 30...
  • Page 31 Create or edit a method. See Section 5.0. • Click Save As. • Click New Folder and enter Folder Name. Figure 5.10 • Click on newly created folder or an existing folder to save method. • Click Save. Figure 5.11 M220 User Manual Page 31...

  • Page 32: Maintenance Screen

    Firmware Updates Covaris Instruments contain computer controllers which are responsible for operating the instrument and communicating with SonoLab. On occasion, it is necessary for Covaris to make changes to the firmware that operates the instrument. When SonoLab connects to an instrument, it checks the version of firmware that is running on that instrument.

  • Page 33: About Tab

    About Tab The About Tab provides user basic information about the instrument such as serial number, software version, board serial numbers etc. See Figure 5.14. Figure 5.14 – About Tab M220 User Manual Page 33...
  • Page 34 EU EMC Directive 2004/108/EC. Also FCC Part 15 Class A radio emissions requirements for the USA and ICES-003 Class A for Industry Canada. AFA-grade Water (Covaris pn 520101 or Highly Purified Water at least Water Bath: ASTM Type III or ISO grade 3) Programmable +6.0 °C to +40.0 °C...

  • Page 35: Troubleshooting

    2. Reboot the computer another application. 3. Restart the system Low water level is detected Objects may be blocking the Clean the water level when water bath is correctly water level sensor. measure channel. filled. M220 User Manual Page 35...

  • Page 36: Appendix A: Suggested M Series Iq / Oq / Pq

    Appendix A: Suggested M Series IQ / OQ / PQ All Covaris instruments are rigorously tested at the Manufacturing Facility prior to shipment. This is done to ensure that your Covaris device is of the highest quality and meets your needs for consistent, efficient, and reliable Sample Preparation.
  • Page 37 Follow the installation manual to assemble the device. Fill the water bath with AFA-grade Water. Verify there are no leaks. Power ON instrument with ON/OFF Switch and verify: 1) Instrument Fans are now audible. M220 User Manual Page 37...
  • Page 38 Verify computer is connected to instrument via USB cable provided. Power on the computer and log in (as required). Note: If Laptop is not provided by Covaris, refer to SonoLab / Driver installation sections of User Manual. Double-click the ‘SonoLab’ shortcut on the computer desktop; verify the application starts and SonoLab main screen successfully displays.
  • Page 39 Method is suspended. Click (Resume) and verify the Method continues where it left off. Verify a ‘Run Results’ Dialog Box is displayed after ~1 minute and indicates “The operation for method ‘Method Test’ completed successfully”. COMMENTS: M220 User Manual Page 39...
  • Page 40  Obtain a tube holder (Covaris #500296) and a Screw Cap microTUBE (Covaris #520096).  Insert the holder into the water bath housing of M220 and fully fill the tube with water and place into the holder.  Specify the above treatment parameters. Denote the Water Bath Temperature and verify it is between 18-22 °C.
  • Page 41 M SERIES – PERFORMANCE QUALIFICIATION (PQ) (2 of 2) DATE: INSTRUMENT S/N: COMPLETED BY: LOCATION: User-Defined Test (As Required) COMMENTS: M220 User Manual Page 41...

  • Page 42: Appendix B: M Series Site Or Transport Preparation

    DO NOT cover the air intake at the front penal. Power Requirements Provide at least two standard outlets, one for the laptop computer, and one for the M220. The M220 draws a maximum of 500 VA. The Covaris M-series instruments are configured with a universal power supply.

  • Page 43: Appendix C: Updating Sonolab Software

    Appendix C: Updating SonoLab Software Latest version of SonoLab can be found in the resource section of Covaris website, http://covarisinc.com/resources/registered-users-login/m-series/ You will need to create an account to access this area of the website Un-Install Existing SonoLab 1. Turn off M220 series device and close SonoLab software application.

  • Page 44: Appendix D: Installing Sonolab On A New Computer And Installing Windows Instrument Drivers

    Generic USB Hub, Covaris RF Controller Board, Covaris RS-485 Port, Covaris Serial Port 2 and Covaris Serial Port 3. Please allow all of these drivers to install and for Windows to confirm successful installation of all drivers before starting SonoLab 7.

  • Page 45: Appendix E: Running The Sonolab Protocol Updater

    Click on the Start menu\ Control Panel\Hardware and Sound. d. Under Devices and Printer, click on Device Manager. The Device manager window will appear. e. Under Other devices, right click first device RF Controller Board and select Update Driver Software. M220 User Manual Page 45...
  • Page 46 When the driver software update dialog opens, select the option to Browse my computer for driver software. g. Point the file open dialog to “c:\Program Files\Covaris\Driver” to find the necessary instrument drivers. Click next. You will be notified that Windows can’t verify the publisher of this driver software.
  • Page 47 Note: When you click on Browse my computer from the Update Driver Software window, the path for the FTDI drivers will remain. No need to re-enter. Go to C:\Program Files\Covaris\Sonolab m. Right Click the Sonolab application. n. Click on Run as Administrator.
  • Page 48 Device Manager under Ports(COM & LPT): • Covaris RF Controller(COM3) • Covaris RF Controller(COM4) • Covaris RF Controller(Port 3&4)(COM5) • Covaris RF Controller(Port 3&4)(COM6) In addition, 4 devices should be added to Device Manager under Universal Serial Bus Controllers: • Covaris RF Controller Board •...
  • Page 49 Appendix E: Running the SonoLab Protocol Updater The SonoLab Protocol Updater is used to update the M220 DNA Shearing Protocols preinstalled on the computer running SonoLab 7. Latest version of the SonoLab Protocol Updater can be found in the resource section of Covaris website, http://covarisinc.com/resources/registered-users-login/m-series/...
  • Page 50 The installer places the protocols in the directory C:\Users\Public\Public Documents\Covaris\SonoLab\Methods\. If the instrument does not perform as expected, please contact Covaris Technical Support: Technical Support – On-going assistance with the operation or application of the equipment and/or troubleshooting is provided via: •...

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