Applied Biosystems QuantStudio 3D Quick Reference page 8

Image the Digital PCR 20K Chips
1. Set the destination for the imaging data:
a. From the Main Menu of the QuantStudio
Instrument, touch Destination.
b. In the Destination screen, touch the desired destination
for the imaging data.
2. If you choose to use a USB drive as a destination for your
imaging data, insert the drive into the USB port on the front
of the instrument.
3. Open the chip tray and load the Digital PCR 20K Chip face-
up into the bay. Confirm that the Digital PCR 20K Chip is
correctly aligned within the chip tray, then close it.
Orient the chip ID and fill port toward the front of the
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instrument.
Confirm that the chip window is clean and correctly aligned
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to the chip.
4. In the instrument touchscreen, touch Start Run, then wait for
the QuantStudio 3D Instrument to image the chip.
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IMPORTANT! Do not open the chip tray or remove the USB
drive (if present) while the instrument displays the
countdown screen. Doing so will invalidate the image data
and require you to repeat the run.
5. When the touchscreen displays the Analyzing Chip screen,
open the chip tray and remove the Digital PCR 20K Chip.
Store or discard it as desired.
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You can read another Digital PCR 20K Chip without waiting
for the analysis to complete. To begin the next run, load the
chip and touch Start Run as described in step 3.
3D
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6. After reviewing the results of the run, touch Done to close
the results screen.
If the analysis of the imaging data produced a
either probe (FAM
for problems and read the chip again.
After a Digital PCR 20K Chip has been read:
• You run the imaged Digital PCR 20K Chip again for up to
1 hour after thermal cycling.
• If you read multiple Digital PCR 20K Chips in rapid
succession, touch the scroll buttons (
results of the previously imaged chips.
• The QuantStudio
analyzed data for the imaged Digital PCR 20K Chip that you
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can access from the Run History screen.
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X0300036
IMPORTANT!
unloaded before you power off and move it. If the instrument is
moved while loaded, the Digital PCR 20K Chip inside can rotate
within the chip tray and obstruct the chip drawer, preventing it
from closing completely.
or VIC dye), visually inspect the chip
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3D Instrument retains a copy of the
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Confirm that the QuantStudio
QuantStudio 3D Digital PCR System Quick Reference
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(red) for
/ ) to review the
3D Instrument is
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