Applied Biosystems QuantStudio 3D Quick Reference page 3

3. Prepare a syringe containing QuantStudio
OpenArray Immersion Fluid:
®
a. Remove the syringe, plunger, and tip from the
packaging.
b. Before uncapping the syringe, gently pull back the
plunger 1-2 mm and release it to break any resistance
that may have formed during storage.
c. Unscrew the cap from the syringe, then attach the
OpenArray Immersion Fluid Tip by pushing it into
®
place.
d. Carefully depress the plunger until Immersion Fluid
flows from the tip of the assembled syringe, then place
it on a clean surface until ready for use.
IMPORTANT!
Use all of the Immersion Fluid within
60 minutes of uncapping the syringe. Once a syringe is
opened, you cannot reattach the cap for later use.
1
OpenArray Immersion Fluid Syringe
®
1
Cap (remove)
2
Immersion Fluid Tip (push to attach)
3
Lint-free wipe
4
4. Load the PCR reaction onto a Digital PCR 20K Chip:
a. Plug in and power on the QuantStudio
Chip Loader, then wait until the Chip Loader status
light illuminates solid green, indicating that the chip
nest has reached operating temperature (£20 minutes
depending on room temperature).
b. Open the QuantStudio
package, then gently grasp the chip by its sides and
load it face-up into the chip nest. Lock the Digital PCR
20K Chip into place by pressing down the chip nest
lever prior to placing the chip into the chip nest as
shown.
IMPORTANT!
Use the image within the chip nest to
correctly orient the Digital PCR 20K Chip within the
Chip Loader.
QuantStudio 3D Digital PCR System Quick Reference
™
12K Flex
™
<1 hour
2 3
3D Digital PCR
™
3D Digital PCR 20K Chip
™
2
1
c. Press the sample loading blade lever, then install the
QuantStudio
the Chip Loader as shown.
IMPORTANT!
the loader head to confirm that the blade is properly
seated.
d. Remove the red protective film from a QuantStudio
3D Digital PCR Chip Case Lid, press the Lid Nest
button, carefully place the Chip Case Lid into the Lid
Nest as shown, then release the button to clamp the
chip in place.
4
e. Briefly vortex and centrifuge the prepared digital PCR
reaction (master mix, assay, and template), then
carefully transfer 14.5 µL of the solution into the
sample-loading port of the Sample Loading Blade. If the
reactions were placed on ice, allow them to warm prior
to loading.
f. Press the black loading button on the Chip Loader to
load the Digital PCR 20K Chip.
The status light flashes green during the loading
sequence, and displays solid green when finished.
g. After the Chip Loader loads the Digital PCR 20K Chip,
immediately add 10-15 drops of Immersion Fluid
directly onto the chip so that the fluid covers the entire
surface. If any fluid is present on the edges of the case
that will contact the lid, remove it with a lint-free wipe
that has been sprayed with isopropanol.
3D Digital PCR Sample Loading Blade to
™
Press the Sample Loading Blade against
Correct
10-15x
Fluid over
chip
1
2
™
1
2
Incorrect
Fluid on Insufficient
edges fluid
3