Error Limits (Nominal Volume, Partial Volume) - BRAND seripettor FEP Operating Manual

Error Limits
Error limits related to the nominal capacity (= maximum volume)
indicated on the instrument, obtained when instrument and distilled
water are equilibrated at ambient temperature (20 °C/68 °F). Testing
takes place with a completely filled instrument and with uniform and
smooth dispensing.
Error limits seripettor
Nominal volume
ml
2
10
25
* A = Accuracy, CV = Coefficient of Variation
Partial volume
The percentage values for A and CV are relative to the nominal volu-
me (V ) and must be converted for partial volumes (V
N
V
N
A
= · A
V
T N
T
e.g. Volume
V
N
V = 50% N
T
V = 10% N
T
* A = Accuracy, CV = Coefficient of Variation
Note:
The maximum error limit for a single measurement can be calculated
EL = A + 2 CV (e.g. for volume 10 ml: 120 µl + 2 x 20 µl = 160 µl).
38
pro
/ seripettor
® ®
A* ≤ ±
µl
%
1.2 24
1.2 120
1.2 300
A* ≤ ±
µl
%
10 1.2 120
5 2.4 120
1 12 120
!
CV* ≤
µl
%
0,2 4
0,2 20
0,2 50
).
T
CV* ≤
µl
%
0,2 20
0,4 20
2,0 20
20 °C
Ex