Reverse Purge (Retrieving Unused Reagents From Injectors, Optional); Flushing The Injectors After Use - Promega GloMax GM2000 Operating Manual

4.B. Reverse Purge (Retrieving Unused Reagents from Injectors, optional)
1. Tap the Start button adjacent to the Reverse Purge option on the Injectors screen (Figure 50).
Figure 52. Reverse Purge function.
2. Ensure the Waste Collection Tray is in position and empty.
3. Tap the Start Reverse Purge button (Figure 52).
4. Any reagents left in the fluid lines will be returned to the reagent container.
4.C. Flushing the Injectors after Use
1. Remove the sample plate from the instrument.
2. Tap the Start button adjacent to the Flush option on the Injectors screen (Figure 50).
Figure 53. Injector Flush function.
3. Ensure the Waste Collection Tray is in position and empty.
4. Tap the Start Flush button (Figure 53).
The recommended flush protocol uses a sequence of 1 cycle (1ml) of deionized water, 1 cycle (1ml) of 70%
ethanol, 1ml of deionized water and then 1 cycle (1ml) of air to flush the fluid.
Note: Empty Waste Collection Tray when priming and flushing are complete. See Section 5.F for injector
cleaning instructions.
38
Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516
TM470 · 4/16 www.promega.com