Promega GloMax GM2000 Operating Manual
  1. Manuals
  2. Brands
  3. Promega Manuals
  4. GPS
  5. GloMax GM2000
  6. Operating manual

Promega GloMax GM2000 Operating Manual

Navigator system
Hide thumbs
Table of Contents

Advertisement

Quick Links

O P E R A T I N G M A N U A L
GloMax
®
Instructions for Use of Products GM2000 and GM2010
Navigator System
4/16
TM470

Advertisement

Table of Contents
loading
Need help?

Need help?

Do you have a question about the GloMax GM2000 and is the answer not in the manual?

Questions and answers

Subscribe to Our Youtube Channel

Summary of Contents for Promega GloMax GM2000

  • Page 1 O P E R A T I N G M A N U A L GloMax Navigator System ® Instructions for Use of Products GM2000 and GM2010 4/16 TM470...

  • Page 2: Table Of Contents

    5.H. Removing or Replacing Inlet and Outlet Tubing ..............43 5.I. Additional Maintenance ..................... 44 Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 www.promega.com TM470 · 4/16...

  • Page 3: Introduction

    Figure 1. The GloMax Navigator System. ® Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM470 · 4/16 www.promega.com...

  • Page 4: Specifications

    EU Declaration of Conformity for the product is available from Promega on request. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 www.promega.com...
  • Page 5 Disk Space 64GB Other Requirements Microsoft.NET Framework 4.0 (full version), Microsoft Excel 2013 Professional Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM470 · 4/16 www.promega.com...

  • Page 6: C Product Components

    1 each Dual Injectors (installed) • 1 each Reagent Bottle Holder • 1 each Waste Collection Tray Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 www.promega.com TM470 · 4/16...

  • Page 7: Precautions And Special Instructions

    6687TA.eps établissement concernant la destruction des accessoires. Clean up spills. Nettoyer les déversements. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM470 · 4/16 www.promega.com...
  • Page 8 Plug instrument and Tablet PC into the same wall socket or outlet strip to assure common grounding. • Do not leave plate in the instrument after protocol is completed. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 www.promega.com TM470 · 4/16...

  • Page 9: Environmental Requirements

    5–30°C, up to 75% humidity Operating Altitude Conditions less than 2,000 meters above sea level Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM470 · 4/16 www.promega.com...

  • Page 10: Inspection And Unpacking

    Remove the instrument and accessory box from the shipping container. Figure 3. Contents of the GloMax Navigator System accessory box. ® Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 www.promega.com TM470 · 4/16...

  • Page 11: Setting Up The Glomax ® Navigator System

    Connect the Instrument AC Power Adapter to the back of the instrument and to a power outlet. Note: We recommend connecting the instrument to an uninterrupted power supply (~500VA). Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM470 · 4/16...

  • Page 12: Switching On The Glomax ® Navigator Instrument

    Tablet is powered ON before the instrument. If that occurs power OFF the Tablet and then power ON the Tablet. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 www.promega.com...

  • Page 13: Shutting Down The Glomax ® Navigator Instrument

    “A1” on the plate tray (Figure 8). Close the sample tray cover and the instrument door. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM470 · 4/16...
  • Page 14 Folder (Section 3.E, Export Folder). If you do not export the results, but want to export them at a later time, you can find the results by touching the Results button on the home screen. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 www.promega.com...

  • Page 15: Glomax ® Navigator User Interface Screens

    Tools. Exit Back Tools Eject Help Figure 10. GloMax Navigator Home screen. ® Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM470 · 4/16 www.promega.com...

  • Page 16: Quick Read Screen

    Navigator Luminescence Quick Read Screen. On the plate map, green wells are ® selected. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 www.promega.com TM470 · 4/16...

  • Page 17: Protocol Screen

    Delete, the user will be asked to confirm the request to delete the user-defined protocol. Figure 13. Delete Protocol message from the GloMax Navigator software. ® Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM470 · 4/16 www.promega.com...
  • Page 18 Save as, Save and Start protocol. Figure 14. GloMax Navigator Protocol Screen. ® Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 www.promega.com TM470 · 4/16...
  • Page 19 Navigator New Protocol Screen showing drag and drop feature to create custom ® protocols. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM470 · 4/16 www.promega.com...
  • Page 20 The plate maps within each step of the protocol are then shaded and cannot be modified. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 www.promega.com...
  • Page 21 Luminescence Integration Times Integration times for luminescence reads may be selected from 0.1 to 10 seconds. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM470 · 4/16...
  • Page 22 Enter between 1 second and 72 hours in increments of 1 second. Figure 22. Wait function screens. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 www.promega.com TM470 · 4/16...
  • Page 23 See Setting Up and Operating the Injector System (Section 4) for more details. Figure 23. Inject Function. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM470 · 4/16...
  • Page 24 Figure 25. Prompt window with the Eject Plate option. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 www.promega.com TM470 · 4/16...
  • Page 25 Rearrange the steps by touching and dragging the appropriate steps. Figure 27. Loop function with with a Luminescence step within the Loop container. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM470 · 4/16...
  • Page 26 Interval Time duration has been reached. Rearrange the steps by touching and dragging the step. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 www.promega.com...

  • Page 27: Results Screen

    Within the plate map, each individual sample may be viewed by tapping the well of interest. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM470 · 4/16...
  • Page 28 Users can use touch-screen gestures to zoom in or out, or to pan the well map. Figure 33. Example of result screen for kinetic data. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 www.promega.com TM470 · 4/16...
  • Page 29 If multiple readings were taken, they each are displayed as you scroll down the page. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM470 · 4/16...
  • Page 30 Workbook. Data with time intervals. Each time value is based on ® when the first read was started (T = 0 seconds). Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 www.promega.com TM470 · 4/16...

  • Page 31: Settings Screen

    Figure 39. Settings  Instrument Info screen located on the Settings menu. Figure 39 shows example information only. Your instrument information may vary. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM470 · 4/16...
  • Page 32 The Injectors may also be accessed from the Tools icon in the menu bar. See Setting Up and Operating the Injector System (Section 4 for more details). Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 www.promega.com...
  • Page 33 Upon tapping the Export Logs button, the logs will export to the Tablet PC at the following location: C:\Exports\Logs Figure 42. Export Logs screen. Figure 43. Export Logs location. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM470 · 4/16 www.promega.com...
  • Page 34 Select Save when finished. Figure 45. Settings  Administrator screen  Export Folder on the Settings menu. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 www.promega.com...
  • Page 35 Select OK when finished. Figure 47. Settings  Administrator screen  Import Protocols. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM470 · 4/16 www.promega.com...
  • Page 36 The context-dependent Help Screen can be accessed from any of the menu screens. Figure 49. Help Screen. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 www.promega.com...

  • Page 37: Operating The Injector System (Optional)

    Note: The reagent bottle holder can accommodate four sizes of containers: a round flat-bottom bottle, a 50ml conical tube, a 15ml conical tube and a 15ml test tube. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM470 · 4/16...

  • Page 38: Priming Injectors

    After priming is complete, empty the Waste Collection Tray. Once the injectors are primed, they are ready for use according to the programmed protocol. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 www.promega.com...

  • Page 39: Reverse Purge (Retrieving Unused Reagents From Injectors, Optional)

    Note: Empty Waste Collection Tray when priming and flushing are complete. See Section 5.F for injector cleaning instructions. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM470 · 4/16...

  • Page 40: Cleaning And Maintenance

    You can dampen the cloth with water or an eyeglass cleaner, but don’t apply liquids directly to the Tablet PC. Don’t use window cleaner or other chemical cleaners on the Tablet PC. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 www.promega.com...

  • Page 41: Cleaning The Optical Head

    Place your hand underneath the Optical Head. Grasp the Aperture and pull it toward you (Figure 56). Note: Do not touch the bottom surface of the Optical Head. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM470 · 4/16...
  • Page 42 Optical Head. Then push down and lock the holder on the left pin (Figure 58). Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 www.promega.com...

  • Page 43: Cleaning The Instrument Interior

    Close the instrument door and turn the instrument ON. The instrument will then return to the Home position and is ready to use. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM470 · 4/16...

  • Page 44: Cleaning The Injectors

    Push the Microplate Sample Tray to the back of the instrument and push the Optical Head from the Home position toward the center of the instrument (Figure 54). Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 www.promega.com...

  • Page 45: Additional Maintenance

    Note: If you suspect fluids have spilled onto the light detector, please contact Promega Technical Services for cleaning instructions. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM470 · 4/16...

  • Page 46: Troubleshooting

    Promega timeout Technical Services to help troubleshoot the issue. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 www.promega.com TM470 · 4/16...

  • Page 47: Common Problems

    Navigator software. If the issue is not resolved, contact Promega Technical Services (techserv@promega.com). Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM470 · 4/16 www.promega.com...
  • Page 48 Remove lid from 96-well plate, place inside the Microplate Sample Tray and close the tray cover. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 www.promega.com...

  • Page 49: Use Of A Usb Flash Drive

    USB flash drive, and restart the Tablet PC and the instrument. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM470 · 4/16...

  • Page 50: Appendix

    Tablet for 3 seconds is equivalent to a Right Click on a normal PC. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 www.promega.com...
  • Page 51 • Select and run preloaded protocols • Build user-defined protocols • View, export results Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM470 · 4/16 www.promega.com...
  • Page 52 OK on the PromegaAdministrator Properties window to add users to the PromegaAdministrators group. Close the Computer Management window. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 www.promega.com...
  • Page 53 PromegaUsers group. Close the Computer Management window. Figure 60. Adding users to the PromegaUsers group. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM470 · 4/16...
  • Page 54 Note: Changing the instrument name will force a reboot of the Tablet PC. Figure 61. GloMax Navigator Software Administrator screen. ® Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 www.promega.com TM470 · 4/16...

  • Page 55: Icons And Symbols

    Favorite. Mark to select a favorite protocol, which will then sort to the top of the page. Yellow favorite indicates selection. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM470 · 4/16...

  • Page 56: Warranty And Service

    Decontamination (see Section 7.D). If the instrument has been exposed to any chemical, biological or radioactive hazards, contact Promega Technical Services for decontamination instructions before shipping. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 www.promega.com...

  • Page 57: Certificate Of Decontamination

    This instrument is certified by the undersigned to be free of radioactive contamination. Date: ____________________________________ Place: ____________________________________ Signature: _________________________________ Name (block capital letters): _______________________________________________________ Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM470 · 4/16 www.promega.com...

  • Page 58: Related Products

    Apoptosis Assays Product Size Cat.# Caspase-Glo 3/7 Assay* 10ml G8091 ® *Additional Sizes Available. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 www.promega.com TM470 · 4/16...
  • Page 59 300 assays (384-well plate) V1501 GloSensor™ cAMP Reagent* 25mg E1290 *Additional Sizes Available. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM470 · 4/16 www.promega.com...
  • Page 60 Product claims are subject to change. Please contact Promega Technical Services or access the Promega online catalog for the most up-to-date information on Promega products. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 www.promega.com...

This manual is also suitable for:

Glomax gm2010

Table of Contents

Save PDF