Operating The Injector System (Optional) - Promega GloMax GM2000 Operating Manual

4.

Operating the Injector System (optional)

Injectors can be used with basic protocols, as well as with all Kinetic and Loop protocols.
Warning: Make sure sufficient volume is available in the well to accept the injected volume. This is especially
!
important for Kinetic and Loop protocols where multiple injection steps may occur. Failure to do so may cause an
overflow of reagent and/or sample into adjacent wells or into the interior of the instrument.
You can define the injection volume and speed, delay time and integration time for each of the two injectors using
the Protocol builder from the Home screen. This information is saved with each protocol.
The viscosity of solutions/reagents can affect the performance of the injectors.
If a protocol requires injectors, the injectors must first be primed with at least 500μl of the reagent to be injected.
Priming wets the fluid path and removes air bubbles to ensure delivery of an accurate injection volume for the
assay.
Priming is also required if an injection protocol has been interrupted for any reason (e.g., power failure).
You may need to optimize injection speed, depending on the solution/reagent you are working with. We recommend
using the default settings of 200µl per second as a starting point. From the Tools icon in the menu bar, or accessed
from Settings  Tools (Figure 38), follow the guided instructions to Prime, Reverse Purge and Flush the injectors.
Figure 50. Injector functions (prime, reverse purge and flush).
After finishing an assay, unused reagent in the fluid path can be retrieved. Select the option to Reverse Purge by
tapping the "Reverse Purge" button on the Injectors screen. To prevent clogging of the fluid lines, flush after each
use.
Note: The reagent bottle holder can accommodate four sizes of containers: a round flat-bottom bottle, a 50ml
conical tube, a 15ml conical tube and a 15ml test tube.
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