Applied Biosystems Data Explorer 4 Series User Manual page 383

Table 9-12 Charge State and Isotope Determination Troubleshooting—Mariner
Symptom
Spectrum peaks labeled
with incorrect charge state
when charge state labels
are selected
(continued)
Monoisotopic peak not
labeled correctly
Peak Detection and Labeling Troubleshooting
Only (Continued)
Possible Cause
Charge state
determination parameters
are set such that peaks
are determined to have no
charge
Noise between isotope
peaks is detected
Peak detection charge
state parameters are not
set correctly, the software
is identifying the tallest
peak in a cluster as the
monoisotopic peak
Data Explorer
Action
Adjust parameters. See:
• "Peak Processing
parameters
(spectrum data only)"
on page 3-26
• Section 3.2.5,
Charge State
Determination and
Examples
Increase %Base Peak
Intensity or %Max Peak
Area to eliminate noise.
See "Basic Settings
(spectrum data only)" on
page 3-22.
Apply noise filter. See
Section 5.7, Noise
Filtering/Smoothing.
Adjust parameters to
correctly identify the
monoisotopic peak. See
"Peak Processing
parameters (spectrum
data only)" on page 3-26.
™
Software User's Guide
9
9-21