Applied Biosystems Data Explorer 4 Series User Manual page 351

Figure 8-5 PSD Processing Dialog Box with
Calibration Tab Displayed
4. Select a PSD Calibration Reference File that you
generated as described in Section 8.3.4, Creating PSD
Calibration Reference (.REF) Files. A calibration
reference file called Angiotensin_Fragments.REF is
provided with the software.
NOTE: Use a calibration reference (.REF) file that
specifies the peak type for reference masses as
Resolved Isotope Mass (even if they are not resolved
isotopes). The calibration routine checks peak width to
determine if a peak matches a Resolved Isotope Mass
or an Average mass. If narrow peaks are specified as
Average Masses in the calibration reference file, the
software mistakes these narrow peaks as isotopically
resolved and ignores the reference mass. For more
information on creating calibration reference files, see
Section 5.3.3, Creating or Modifying a
Calibration Reference File (.REF).
Data Explorer
Calibrating a PSD Spectrum
™
Software User's Guide
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