Agilent Technologies 1290 Infinity II User Manual page 47

Solvent Recommendation for Agilent 1290 Infinity and 1290 Infinity II and 1260 Infinity II Flexible Pumps
Handling of Buffers
The following recommendations should be observed when using buffer
solutions:
• Buffers and aqueous solutions are possible sources of algae contamination,
• For buffer concentrations of 0.1 M or higher using the seal wash function
• Filter buffer solutions to avoid increased wear or blockages that are caused
• Avoid conditions where mixing of buffers and organic solvents may cause
• Use a minimum flow rate of 5 μL/min or 1 % composition per solvent
• Consider using an inline filter, for example Inline filter (G1311-60006).
• Never leave buffers in a system without flow. Before shutting down a
• Regularly maintain the LC system.
1290 Infinity II High Speed Pump User Manual
for avoiding related problems, please read
Systems" on page 49.
periodically with a runtime of 0.3 min every 3 min is strongly
recommended.
by undissolved crystals. Always use solvent inlet filters.
precipitation, as this impairs the reproducibility of chromatographic
experiments and may also reduce the system life time. For example in
reversed phase chromatography, avoid buffers (especially phosphate
buffers) with a concentration higher than 20 mmol/L. For phosphate
buffers, avoid compositions containing more than 65 % acetonitrile or other
organic solvents.
channel (whatever is greater) to avoid cross-flow. Cross-flow can be caused
by micro leaks in pump heads and can result in buffer precipitation in
pump heads, channel blocking, or reduced pump head life time through
wear of seals and pistons.
system, flush it extensively with warm water to avoid clogging of valves,
capillaries, or flow cells or reducing the life time of your column. If the
system is not used for some time, for example more than a day depending
on lab temperature, fill all solvent lines with organic solvent or water with
at least 10 % isopropanol.
Using the Pump
"Algae Growth in HPLC
3
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