The Detector Parameters - Agilent Technologies 1100 Series Reference Manual

Semimicro flow cell
Micro flow cell kit
High Pressure flow cell
However you have to be aware that there are additional tolerance of gasket thickness and its compression
ratio which is supposed to be very small in comparison with the machining tolerance.
Set the Detector Parameters
5 µl
1 µl
14 µl
1 Set peakwidth as close as possible to the width (at half height) of a narrow peak of
interest.
2 Choose the sample wavelength.
•
at a longer wavelength than the cut-off wavelength of the mobile phase,
•
at a wavelength where the analytes have strong absorptivity if you want to get the
lowest possible detection limit,
•
at a wavelength with moderate absorptivity if you work with high concentrations,
and
•
preferably where the spectrum is flat for better linearity.
3 Consider to use time-programming to further optimization.
G1314-60083 6 mm
G1314-60081 5 mm
G1314-60082 10 mm
6.10 ± 0.19 mm
4.80 ± 0.19 mm
10.00 ± 0.19 mm
How to optimize the detector
6/6.19
5/4.8
6/5.75
2