Agilent Technologies 1260 Infinity User Manual page 63

Fused Silica Capillary Issues
• When connecting a capillary (especially at the column) press it smoothly
The quartz core of PEEK/fused silica capillaries will crack and debris will clog the flow path
N O T E
if the fittings are overtightened. Fittings shouldn't be tightened harder than finger tight plus
1/4 turn with a wrench.
• Be careful when bending fused silica capillaries. The diameter must not be
• When you replace a part, especially a capillary, clean it with acetone.
• If a fused silica capillary leaks, do not retighten the fitting under flow. Set
• Avoid the use of alkaline solutions (pH > 8.5 ) as they attack the fused silica
• Be careful not to crush capillaries when closing module doors.
• A broken capillary can release silica particles into the system (e.g. cell)
• Often, a clogged capillary can be recovered by backflushing with acetone.
1260 Nanoflow Pump User Manual
into the fitting to avoid void volumes. Incorrect setting will result in
dispersion, causing tailing or footing peaks.
smaller than 40 mm.
the column flow to zero, re-insert the capillary, tighten and set the new
column flow.
of the capillaries.
causing problems in the system downstream of the crack.
Hints For Successfully Using the Pump
4
Using the Pump
63