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Operation - National 131 Instruction Manual

Compound microscopes
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For optimum viewing satisfaction, follow these simple procedures. Nomenclature used to describe components and
controls can be identified by referring to the diagram at left.
UNPACKING
1. For Models 131 & 132, carefully remove microscope, dustcover and for Models 131-CLED & 132-CLED also remove
a "L" hex wrench. Always handle and move microscope by securely holding the arm of microscope. Avoid touching
any of the lens surfaces while handling the microscope. Dust, dirt, or fingerprints can damage the delicate lens
surfaces or adversely affect image quality.
2. Examine packing material before you discard it. Retain the styrofoam container in case you need to transport,
store, or return the microscope for service. If it becomes necessary to ship the microscope for any reason, pack it
in the styrofoam container, and then pack the styrofoam in another corrugated shipping container for optimum
protection. Use of the styrofoam alone will not provide adequate protection in transit, and will void your warranty.
DESCRIPTION OF COMPONENTS
1. EYEPIECE (ocular lens) Lens closest to the eye, magnifies the primary image formed by the objective lens. Dual
head models 132 and 132-CLED feature both an inclined eyepiece (for primary viewing) and a vertical eyepiece for
second viewer or for mounting a camera. The inclined eyepiece is equipped with a "pointer" that rotates as the
eyepiece is turned.
2. DIOPTER ADJUSTMENT (on models 132 and 132-CLED only) Permits focusing adjustment of image for any
difference in vision between primary and secondary viewers.
3. OBJECTIVE TURRET (nosepiece) Revolving turret which holds objective lenses, permits changes of magnification
by rotating different powered objective lenses into optical path.
4. OBJECTIVE LENS
5. STAGE CLIPS Two locked-on clips hold specimen slide in place on stage. Note: Your microscope is already drilled
and tapped to accept an optional mechanical stage. Mechanical stage replaces stage clips and permits precise,
mechanical manipulation of the specimen slide.
6. STAGE
Platform of the microscope where the specimen slide is placed.
7. CONDENSER LENS
illumination and fills the back lens element of objective lens to improve image resolution.
8. DISC DIAPHRAGM
optimum resolution of the objective lens. Larger apertures used for higher magnifications, and smaller apertures used
for lower magnifications.
9. SAFETY RACK STOP
objectives from breaking specimen slides, prevents damage to objective lenses. This stop has been pre-adjusted at
the factory.
10. FOCUSING KNOBS
bring specimen image into focus. Fine focus knobs (smaller knobs located just below coarse focusing knobs) permit
more precise image adjustment.
11. ILLUMINATION
Models 131 & 132 have a built-in substage electric illuminator and Models 131-CLED & 132-CLED
have a built-in substage electrical LED illuminator that provides constant, reliable, pre-focusing illumination.

OPERATION

1. Place microscope directly in front of you in a manner which permits you to comfortably look into the eyepiece. Note
that the head of microscope rotates 360º, permitting you to operate the microscope from the front or from the back,
whichever is most convenient for you. It also permits convenient sharing of microscope by more than one user, by
simply rotating head, without needing to move entire microscope. Most users will position the microscope with the
arm facing them so that focusing knobs are most convenient to reach.
Lens closest to the object being viewed, forms first magnified image of the specimen.
An 0.65 N.A. condenser lens, fixed in center of stage, condenses light rays from substage
Rotating disc located below stage, with holes of various apertures, designed to help achieve
When properly adjusted, controls maximum upward travel of stage. Prevents higher power
Coarse focusing knobs (larger knobs) located on each side of arm, raise or lower stage to
3

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