Leica EM UC6 Operating Manual
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Leica EM UC6 Operating Manual

Leica EM UC6 Operating Manual

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Order Number 16200032
Leica EM UC6 - E - 05/04
Leica EM UC6
Operating Manual

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Summary of Contents for Leica EM UC6

  • Page 1 Leica EM UC6 Operating Manual Order Number 16200032 Leica EM UC6 - E - 05/04...

  • Page 2: Important Note

    Important Note Leica reserves the right to change technical specifications as well as manufacturing processes without prior notice. Only in this way is it possible to continuously improve the technology and manufacturing techniques used to provide our customers with excellent products.
  • Page 3 Leica EM UC6 Operating Manual Leica EM UC6 Serial Number: Date of purchase: For the instrument serial number, please refer to the name type label on the back of the instrument! Please read this instruction manual carefully before operating the instrument.

  • Page 4: Table Of Contents

    Table of Contents Instruction Installation of the UC6 Installation of the antivibration table 2.1.1 UC6 built in antivibration system 2.1.2 Removal of transport lock Mounting of the stereo microscope 2.2.1 Mounting the breath shield of the S6 or S4 stereo microscope 2.2.2 Mounting the breath shield of the MZ6 stereo microscope Mounting the room temperature items 2.3.1 Knife stage...

  • Page 5: Safety Instructions

    Extreme caution is advised when using the razor blade ! For safety, Leica recommends the use of a trimming machine. Symbols in this manual and their meaning: Attention, take extra care.

  • Page 6: Installation Of The Uc6

    2. Installation of the UC6 When the instrument is supplied with a working table the UC6 is packed in a special carton fastened below the working table . When the UC6 is supplied without working table it is housed in a second carton. Cut open the first carton on the top and take out the second carton.
  • Page 7 Remove the rear foam pad. Remove the front foam pad. Put left -and right hand pads to the side. Remove plastic bag covering the instrument. Two persons should lift the instrument. Important: The instrument has to be lifted only by means of the lateral carrying ledges on the base plate.

  • Page 8: Installation Of The Antivibration Table

    2.1 Installation of the antivibration table The instrument table with its special antivibration system is delivered assembled. By using this table the UC6 built-in antivibration system will be disabled. Remove the foam transportation lock from the antivibration insert. Remove Allen key and unscrew retaining screws from the cross beam.

  • Page 9: Uc6 Built In Antivibration System

    After attaching the transport lock the hand wheel must not be rotated. Shipment without transport lock as well as without special carton may cause damage. The special carton and the transport lock with packing hints is available from your local Leica Agency or from Leica Microsystems GmbH, A-1170 Vienna, Austria.

  • Page 10: Mounting Of The Stereo Microscope

    2.2 Mounting of the stereo microscope Insert stereo microscope in the adaptor and clamp it with the knurled screw in front of the adapter ring. 2.2.1 Mounting the breath shield of the S6 or S4 stereo microscope Screw in one of the provided slot screws on the side of the stereo adaptor.

  • Page 11: Mounting The Room Temperature Items

    2.3 Mounting the room temperature items 2.3.1 Knife stage Place the knife stage on the support. Clamp it with the locking lever. 2.3.2 Segment arc Insert the segment arc into the specimen arm and clamp it with the knurled screw. 2.3.3 Peristaltic pump The peristaltic pump can be mounted either on the left hand side or on the right hand side of the...

  • Page 12: Mounting The Antistatic Electrode For Room Temperature Work

    2.3.4 Mounting the antistatic electrode for room temperature work Insert the electrode into the cover of the stereo carrier. Pull the cable of electrode out of the cable channel. Mount the gooseneck on the stereo adapter of the S6 or S4 stereo microscope.

  • Page 13: Control Unit (Electrical Connections)

    2.4 Control Unit (electrical connections) Power supply cable with retaining screw has to be tightened on the socket UC6 cable. Tighten the plug with the two screws onto the socket of the control unit. UC6 cable. Tighten the plug with the two screws on the socket of the UC6 body.

  • Page 14: Operation Of The Touch Sensitive Controller

    3. Operation of the touch sensitive controller Environmental specifications of the touch screen: Operating temperature: 0°C to 60°C (no condensation) Operating humidity: less than 90% RH (no condensation) Storing temperature: -20°C to 70°C (no condensation) Storing humidity: less than 95% RH (no condensation) Cleaning of the touch screen can be carried out with a commercially available damp, lint-free cloth .
  • Page 15 HELP: by touching this button followed by the button in question on the touch screen the appropriate function will be described. CUTTING WINDOW: set the cutting window with buttons START and END The length of the cutting window on the UC6 has to be set the following way: Turn the handwheel so the specimen is in the forward cutting stroke.
  • Page 16 ANTISTATIC Control: As soon as the antistatic device is connected the control area will be displayed on the screen. - decreases the intensity of the electrode + increases the intensity By touching Antistatic ON/OFF control button fades to grey. APPROACH: of the knife towards the specimen Coarse control with the track wheel and jog control by using STEP buttons.
  • Page 17 ILLUMINATION: The UC6 is equipped with three different types of LED illumination. Toplight ON/OFF The intensity of each light switched on can by controlled by using the + or — button. Transillumination ON/OFF After switching off the light the value of the Backlight ON/OFF intensity is stored.
  • Page 18 E-W MEASUREMENT: the Integrated E-W measuring system offers the possibility to determine the size of the block face and inclusions. Measuring procedure: Use the corner of the knife as a reference • Move the knife to the starting point of measurement •...
  • Page 19 By using a 45° beveled trimming knife blade it has to be taken into account that the specimen width will be reduced by approx. 2.5x of the trimming depth, e.g. in order to receive a 500µm blockface-size with a total depth of trimming of 200µm the entered specimen width should be 1000µm.
  • Page 20 Every step can be interrupted by pressing the green RUN/STOP button. An information window appears on the left corner. By pressing the START button of the AUTOTRIM window the pre-programmed trimming cycle starts from the 1st step onwards. By pressing the green RUN/STOP button the trimming cycle will be resumed. By pressing the CLEAR button a new program can be written or MODE to leave AUTOTRIM, Once the blockface, the right and left edge of the sample has been trimmed, the knife moves to the right edge of the sample.
  • Page 21 The total depth of trimming for the upper and lower edges are taken over from step 4. After pressing the START button of step 8 the instrument starts trimming the last two edges. Once the upper and the lower edge of the sample is trimmed an acoustic signal is given.
  • Page 22 MENU: by touching this button up to seven different user/ sample profiles can be set and recalled by pressing the corresponding name of the user or sample. Every parameter e.g. brightness of the illumination, speed/feed, memory or the AutoTrim setting are stored for each profile. RENAME: key in the name of the operator or sample.

  • Page 23: Operation Of The Key-Pad Controller

    4. Operation of the key-pad controller FC6 Control buttons with 3 memories and storage of temperature. Graphic Display indicates Ioniser control, START, HEAT, selected step size of the LN2 STOP and Alarm OFF buttons. In conjunction with FC6 Cryochamber Buttons for Step Size setting of the approach.
  • Page 24 CUTTING WINDOW: Move the lower edge of the blockface by means of the handwheel to just above the knife edge. Press the button CUT START. Move the blockface down until the upper edge of the block face is below the knife edge . Press the button CUT END.
  • Page 25 ILLUMINATION: the UC6 is equipped with three different types of LED illumination. Top light for observation of the cutting process and judgment of the section thickness. Transillumination for locating and identifying interesting structures within the specimen block when trimming with the MESACUT or with the trimming block.
  • Page 26 4.1 SUBMENU: By pressing the alarm off button longer than 1 second you enter the submenu. To leave it press button once again. Note: the alarm button is normally used in conjunction with the FC6 cryochamber (described in the FC6 manual). Use CUT START and CUT END button to select desired parameter which would like to be changed.

  • Page 27: Description Of The Instrument

    5. Description of the Instrument The following instructions for the use of the instrument will mediate all information of the proper use and maintenance of the Ultramicrotome. May we suggest you familiarize yourself with the operating elements and run through the following manipulations before starting sectioning.

  • Page 28: Transillumination

    5.3 Transillumination The transillumination is connected with the connecting cable and its plug to the outlet. The other side with the LED is inserted into the swivelling part of the segment arc. Swivel the arc into the upper position and insert the LED mounting, clamping screw (plastic head) facing you.

  • Page 29: Drive System

    If the specimen arm is in its retracted mode the segment on the display lights up. 5.7 Stereo Carriers 5.7.1 with eucentric movement of the stereo microscope The eucentric movement of the Leica EM UC6 viewing system allows examination of sections, even with a lowered water level (e.g. for Lowycryls) and dry sections.

  • Page 30: Technical Specification

    6. Technical specification c i f ° 5 ° 5 i r r - / ° ° 8 t l i s i t c i t g i l g i l u l l t t u y t i µ...

  • Page 31: Ec Declaration Of Conformity

    Hernalser Hauptstrasse 219 A-1170 Wien Austria declare in exclusive responsibility that the product erklären in alleiniger Verantwortung, daß das Produkt déclarons sous notre seule responsabilité que le produit Model Leica EM UC6 Modell Leica EM UC6 Modèle Leica EM UC6 705801/655825...
  • Page 32 Leica Mikrosysteme GmbH Hernalser Hauptstrasse 219 A-1170 Vienna Phone:+43 1 48899 Fax: +43 1 48899 350 www.em-preparation.com...

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