Zeiss 35 mm SLR Camera Operating Manual
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Zeiss 35 mm SLR Camera Operating Manual

Zeiss 35 mm SLR Camera Operating Manual

Photomicrography with 35 mm slr cameras
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Photomicrography with
35 mm SLR Cameras
Operating Manual

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Summary of Contents for Zeiss 35 mm SLR Camera

  • Page 1 Photomicrography with 35 mm SLR Cameras Operating Manual...

  • Page 2: Introduction

    INTRODUCTION Photomicrography using Carl Zeiss Copyright 35 mm SLR cameras Knowledge of this manual is required for the operation of the instrument. Would you therefore please make yourself familiar with the contents of this manual and pay special attention to hints concerning the safe operation of the instrument.

  • Page 3: Table Of Contents

    Overall View of 35 mm SLR camera on Adapter for SLR Camera of the the Stemi 2000-C with 60 mm Camera Port ..............0-8 Overall View of 35 mm SLR Camera on 2.5 / T2 Camera Adapter on the Front-Port of the Axiovert 25 C....................0-9...
  • Page 4 INTRODUCTION Photomicrography using Carl Zeiss Contents 35 mm SLR cameras CHAPTER 2 START-UP........................2-1 Start-Up ........................2-2 Unpacking the Photomicrography Components............2-2 Insertion of Photo Reticles MC 2.5 ................2-2 2.2.1 Assignment of Photo Reticles MC 2.5 ................ 2-2 2.2.2 Insertion of MC 2.5 Photo Reticles in the Eyepiece .............
  • Page 5 Photomicrography using INTRODUCTION 35 mm SLR cameras Contents Carl Zeiss CHAPTER 4 MAINTENANCE, CARE, TROUBLESHOOTING AND SERVICE ........4-1 Maintenance, Care, Troubleshooting and Service ............4-2 Instrument Care ......................4-2 Instrument Maintenance .....................4-3 4.2.1 Performing Checks...................... 4-3 4.2.2 Changing the Batteries....................4-3 Troubleshooting......................4-3...

  • Page 6: Notes On Instrument Safety

    INTRODUCTION Photomicrography using Carl Zeiss Instrument safety 35 mm SLR cameras Notes on Instrument Safety The following warning and information symbols are used in this manual: NOTE This symbol is a warning which you must observe under all circumstances. CAUTION This symbol is a warning which indicates a hazard to the instrument or instrument system.

  • Page 7: Notes On Warranty

    35 mm cameras may be performed. Repairs may only be performed by Carl Zeiss service staff or specially authorized personnel. Should any defect occur with the instrument, please get in touch with the Carl Zeiss microscopy service in Germany (see page 4-4) or your local Carl Zeiss agency.
  • Page 8 Photomicrography using Carl Zeiss Overall view 35 mm SLR cameras Overall View of 35 mm SLR Camera on 2.5 /T2 Adapter for SLR Cameras on 60 mm Camera Port of the Stemi 2000-C Stereomicroscope in this Example Cable release Eyepiece cover...

  • Page 9: Of The Axiovert 25 C

    INTRODUCTION 35 mm SLR cameras Overall view Carl Zeiss Overall View of 35 mm SLR Camera on 2.5 / T2 Adapter on Front Camera Port of the Axiovert 25 C front camera port 2.5 / T2 camera adapter T2 adapter...
  • Page 10 Carl Zeiss Overall view 35 mm SLR cameras Overall View of 35 mm SLR Camera on 63 mm Objective and Adapter for Microscope Camera (Image Position 44 mm) with 10 Camera Eyepiece on 44 mm Camera Port – i.e. on the...

  • Page 11: Chapter 1 Instrument Description

    Name and Intended Application ..................1-3 Instrument Description and Main Features................1-3 35 mm SLR Camera Components..................1-4 Overview of Image Positions (Tube Adapters), relevant Phototubes and Microscope Stands......................1-7 Technical Data .........................1-9 List of illustrations Fig. 1-1 35 mm SLR camera components ..................1-4 B 40-046 e 03/99...
  • Page 12 INSTRUMENT DESCRIPTION Photomicrography with Carl Zeiss 35 mm SLR cameras B 40-046 e 03/99...

  • Page 13: Instrument Description

    Instrument Description Carl Zeiss INSTRUMENT DESCRIPTION Name and Intended Application The use of commercially available 35 mm SLR cameras on microscopes from Carl Zeiss is a low-price alternative to photo documentation using the MC 80 and MC 200 microscope cameras.

  • Page 14: 35 Mm Slr Camera Components

    INSTRUMENT DESCRIPTION Photomicrography with Carl Zeiss Components 35 mm SLR cameras 35 mm SLR Camera Components Fig. 1-1 35 mm SLR camera components B 40-046 e 03/99...
  • Page 15 INSTRUMENT DESCRIPTION 35 mm SLR cameras Components Carl Zeiss Key to Fig. 1: 35 mm SLR camera housing T2 adapter Objective f’ = 63 mm in T2 mount 2.5 /T2 adapter for SLR cameras 2.5 SLR adapter comp. 2.5 / T2 camera adapters...
  • Page 16 Contax Aria including eyepiece cover 000000-1057-127 Ricoh XR - X 3000 (mainly for use with Axiovert microscopes) 416116-0000-000 Praktica BX 20 35 mm SLR camera housing (mainly for use with Axiovert microscopes) 000000-0219-086 Cable release for Contax 167 MT and Contax Aria 416167-0000-000...

  • Page 17: Overview Of Image Positions (Tube Adapters), Relevant Phototubes And Microscope Stands

    Photomicrography with INSTRUMENT DESCRIPTION 35 mm SLR cameras Technical Data Carl Zeiss Overview of Image Positions (Tube Adapters), relevant Phototubes and Microscope Stands Image Position Cat.No. of Phototube Microscope Stand (Tube Adapter) 60 mm 452902-0000-000 KF 2, Standard 20 / 25...

  • Page 18: Instrument Description

    INSTRUMENT DESCRIPTION Photomicrography with Carl Zeiss Technical Data 35 mm SLR cameras Image Position Cat.No. of Phototube Microscope Stand (Tube Adapter) 44 mm 452910-0000-000 Axioskop / Axioskop 20 452911-0000-000 452912-0000-000 452914-0000-000 451722-0000-000 Axiovert 10 / 35 / 35 M 452920-0000-000...

  • Page 19: Technical Data

    35 mm SLR camera housing Contax 167 MT ................0.620 kg 35 mm SLR camera housing Contax Aria ................... 0.460 kg 35 mm SLR camera housing Ricoh XR - X 3000 ................. 0.480 kg 35 mm SLR camera housing Praktica BX 20 ................0.625 kg Adapter bayonet / M 42 1 thread ...................
  • Page 20 Carl Zeiss Technical Data 35 mm SLR cameras Power supply for 35 mm SLR camera housing Contax 167 MT ....................4 batteries AAA / 1.5 V Contax Aria......................2 batteries CR 2 / 3 V Ricoh XR - X 3000 ................4 batteries AA / 1.5 V (LR 6 / LR 03) Praktica BX 20 ..............

  • Page 21: Start-Up

    Photomicrography with START-UP 35 mm SLR cameras Contents Carl Zeiss START-UP Contents START-UP........................2-2 Unpacking the Photomicrography Components..............2-2 Insertion of Photo Reticles MC 2.5 ..................2-2 2.2.1 Assignment of Photo Reticles MC 2.5 ................2-2 2.2.2 Insertion of MC 2.5 Photo Reticles in the Eyepiece ............2-4 2.2.3...

  • Page 22: Unpacking The Photomicrography Components

    Carl Zeiss service staff against payment. Unpacking the Photomicrography Components The photomicrography components from Carl Zeiss are delivered in separate packaging. Remove all components from the packaging and use the delivery note to check for completeness. Properly dispose of original packaging, or keep it for storage or return of the instrument to the manufacturer.
  • Page 23 Photomicrography with START-UP 35 mm SLR cameras Photo reticles Carl Zeiss Photo Reticles Cat. No. Eyepieces Cat. No. MC 2.5 / d = 26 mm 454075-0000-000 PL 10 / 18 Br. foc. 444132-9901-000 * E-PL 10 / 20 Br. foc.

  • Page 24: Insertion Of Mc 2.5 Photo Reticles In The Eyepiece

    SLR camera. The MC 2.5 photo reticles are inserted into the eyepieces 10 foc. of the relevant microscope. The photo reticles (2-2/1) from Carl Zeiss have been adhered to screw-in mounts (2-2/2) to allow easy replacement.

  • Page 25: Attachment Of 35 Mm Slr Cameras

    START-UP 35 mm SLR cameras Attachment of 35 mm SLR cameras Carl Zeiss Attachment of 35 mm SLR Cameras The attachment of 35 mm SLR cameras to various camera ports is described in detail in the following and can be performed by the users themselves.

  • Page 26: Attachment Of 35 Mm Slr Cameras With Objective F' = 63 Mm To 60 Mm And 40 Mm Camera Ports

    START-UP Photomicrography with Carl Zeiss Attachment of 35 mm SLR cameras 35 mm SLR cameras 2.3.2 Attachment of 35 mm SLR Cameras with Objective f' = 63 mm to 60 mm and 40 mm Camera Ports Remove dust cover or lens from the SLR camera housing.

  • Page 27: Attachment Of 35 Mm Slr Cameras With 2.5 / T2 Adapter To Front Port Of Axiovert 25 C / Cfl / Ca

    Photomicrography with START-UP 35 mm SLR cameras Attachment of 35 mm SLR cameras Carl Zeiss 2.3.3 Attachment of 35 mm SLR Cameras with 2.5 / T2 Adapter to Front Port of Axiovert 25 C / CFL / CA Remove dust cover or lens from the SLR camera housing.

  • Page 28: Attachment Of 35 Mm Slr Cameras To Front Camera Port Of Axiovert 35 / 35 M / 135 / 135 M

    START-UP Photomicrography with Carl Zeiss Attachment of 35 mm SLR cameras 35 mm SLR cameras 2.3.4 Attachment of 35 mm SLR Cameras to Front Camera Port of Axiovert 35 / 35 M / 135 / 135 M The front camera port (2-6/5) of the Axiovert 35 / 35 M / 135 / 135 M is a T2 adapter with camera factor 2.5 attached to the stand.

  • Page 29: Attachment Of 35 Mm Slr Cameras With Objective F' = 63 Mm To Camera Ports With

    Photomicrography with START-UP 35 mm SLR cameras Attachment of 35 mm SLR cameras Carl Zeiss 2.3.5 Attachment of 35 mm SLR Cameras with Objective f' = 63 mm to Camera Ports with 40 mm Clamping Diameter For photomicrography with 35 mm SLR cameras on Standard, WL and IM microscope stands with...

  • Page 30: Determination Of The Exposure Time Range For Unblurred Photographs

    START-UP Photomicrography with Carl Zeiss Attachment of 35 mm SLR cameras 35 mm SLR cameras Determination of the Exposure Time Range for Unblurred Photographs The limits of the exposure time range for unblurred photographs depend on the used SLR camera, the microscope adaptation, the microscope magnification and the microscope stand to which the camera is attached.

  • Page 31: Operation

    Fig. 3-1 Use of bulk film .......................3-5 Fig. 3-2 Measuring range of 35 mm SLR camera, ISO 100 / 21°, camera lens f / 1.4 with apertures k = 1.4 - 16 ....................3-8 Fig. 3-3 Measuring range of 35 mm SLR camera with T2-Adapter, ISO 100 / 21° ......3-9...

  • Page 32: Operation

    Further information on the use of 35 mm SLR cameras for photomicrography are given in section 3.2, photomicrography with SLR cameras. The following section will not deal with the setting of microscopes for photomicrography. The necessary steps are described in the relevant operating manuals of the microscopes from Carl Zeiss. B 40-046 e 03/99...

  • Page 33: Photomicrography With 35 Mm Slr Cameras

    Set exposure correction for illumination technique. also see section 3.2.4, exposure correction depending on microscopy technique. If a suitable data back is used with the 35 mm SLR camera: enter the data. also see the operating instructions of the used data back.

  • Page 34: Setting The Color Temperature To 3200 K And Setting The Image Brightness

    OPERATION Photomicrography with Carl Zeiss Selection of color temperature/film 35 mm SLR cameras 3.2.2 Setting the Color Temperature to 3200 K and Setting the Image Brightness When using color reversal films for artificial light (3200 K), it is recommended to set the illuminator to the color temperature 3200 K (approx.

  • Page 35: Exposure Correction Depending On The Microscopy Technique

    Photomicrography with OPERATION 35 mm SLR cameras Image section/Exposure functions Carl Zeiss The film cuts must always be performed as stipulated in DIN 4536 or ISO 1977 (also see Fig. 7 ... 9 P 3-1). The film should be cut so that none of the 23 mm perforation holes are cut open.

  • Page 36: Setting And Focusing Of Image Section

    The exposure correction ring has been set to +1. The first photo is exposed with +1 EV, the second with 0 EV and the third with -1 EV. For the setting of exposure correction and exposure bracketing please see the operating instructions of the used 35 mm SLR camera! 3.2.5 Setting and Focusing of Image Section Via binocular tube;...

  • Page 37: Exposure Functions

    OPERATION 35 mm SLR cameras Image section/Exposure functions Carl Zeiss If the structures of microprism ring and split image indicator are not seen in focus, attach the special eyepiece for eyeglass wearers to the viewfinder. Fine object structures and details can be focused in accordance with the image in the clear glass areas of the split image indicator.
  • Page 38 Exposure functions 35 mm SLR cameras Fig. 3-2 Measuring range of 35 mm SLR camera, ISO 100 / 21°, camera lens f / 1.4 with apertures k = 1.4 - 16 Example 3: The camera in Fig. 3-2 measures shutter speeds between T...
  • Page 39 The measuring range of cameras with T2 adapter is determined by the ranges of exposure values (EV), shutter speeds, film sensititvity and by the open aperture 1.2 or 1.4. Fig. 3-3 Measuring range of 35 mm SLR camera with T2-Adapter, ISO 100 / 21° B 40-046 e 03/99...

  • Page 40: Trigger The Exposure

    SLR before the automatic exposure is triggered. For the setting of the various measuring techniques and exposure modes, and for the display of exposure times, please see the operating manual of the used 35 mm SLR camera. 3.2.7...

  • Page 41: Further Notes

    Photomicrography with OPERATION 35 mm SLR cameras Blurs/Color balance Carl Zeiss 3.2.8 Further Notes 3.2.8.1 Avoid Blurs Mirror and shutter functions of the SLR camera can cause vibration of the photomicrography structure resulting in blurred micrographs. The image will be dull, the object structures washed out. This is...
  • Page 42 OPERATION Photomicrography with Carl Zeiss Color balance 35 mm SLR cameras Assessing the Color Balance Make test exposures of a specimen area with as much empty background as possible in transmitted- light brightfield. View slides on a standard light box, the light source of which has the correct illuminance and the spectral energy distribution of 5500 K.

  • Page 43: Darkfield And Fluorescence Photography

    Photomicrography with OPERATION 35 mm SLR cameras Darkfield and fluorescence photography Carl Zeiss 3.2.8.3 Darkfield and Fluorescence Photography Compared to standard photomicrography, the following special conditions apply to darkfield and fluorescence photography: The low brightness level frequently encountered calls for long exposure times. In the case of low brightness, switch the beam splitter on the phototube to "100% of light to camera port".

  • Page 44: Computing The Magnification

    OPERATION Photomicrography with Carl Zeiss Color balance 35 mm SLR cameras 3.2.8.4 Computing the Magnification SLR connections, 2.5 / T2 adapters: The magnification of the image on the 35 mm film M is the product of objective magnification M film and the factor of the T2 adapter 2.5 .

  • Page 45: Using Digital Cameras

    If the outer dimensions of the sensor chip are 9.3 mm 14 mm, the image contents of the digital photo totals approx. 2/5 of a photo on 35 mm film. Further information on the use of digital cameras is available from your local Carl Zeiss agency or from the following Internet address: micro@zeiss.de...
  • Page 46 OPERATION Photomicrography with Carl Zeiss Color balance 35 mm SLR cameras 3-16 B 40-046 e 03/99...

  • Page 47: Maintenance, Care, Troubleshooting And Service

    Photomicrography with MAINTENANCE, CARE, TROUBLESHOOTING AND SERVICE 35 mm SLR cameras Contents Carl Zeiss MAINTENANCE, CARE, TROUBLESHOOTING AND SERVICE Contents MAINTENENANCE, CARE, TROUBLESHOOTING AND SERVICE ........4-2 Instrument Care.......................4-2 Instrument Maintenance ....................4-3 4.2.1 Performing Checks ......................4-3 4.2.2 Changing the Batteries ....................4-3 Troubleshooting ......................4-3...

  • Page 48: Maintenance, Care, Troubleshooting And Service

    MAINTENANCE, CARE, TROUBLESHOOTING AND SERVICE Photomicrography with Carl Zeiss Instrument care 35 mm SLR cameras MAINTENENANCE, CARE, TROUBLESHOOTING AND SERVICE Instrument Care Maintenance of the photomicrography components attached to the microscopes is limited to the following operations: Switch off the instrument after every use and place instrument cover on it to protect it from dust and humidity.

  • Page 49: Instrument Maintenance

    SLR camera (e.g. by flashing of the display). If any damage is visible, switch off the SLR camera. Repairs may only be performed by authorized service staff; therefore, please contact the Carl Zeiss service department (see page 4-4). 4.2.2 Changing the Batteries See the relevant notes in the manual supplied by the camera manufacturer.

  • Page 50: Consumables And Tools

    459312-0000-000 to cover the instrument after use Requesting Service All repairs of mechanical, optical or electronic components of the 35 mm SLR camera may only be performed by Carl Zeiss service staff or specially authorized personnel. To ensure the optimum setting and trouble-free function of the components even for a longer period of time, we would recommend you to conclude a service/maintenance contract with Carl Zeiss.

  • Page 51: Annex

    Photomicrography with ANNEX 35 mm SLR cameras Contents Carl Zeiss ANNEX List of Abbreviations ......................... A-2 Physical and Technical Units ......................A-3 List of Key Words..........................A-4 EC Declaration of Conformity ......................A-5 B 40-046 e 03/99...

  • Page 52: Annex

    ANNEX Photomicrography with Carl Zeiss List of abbreviations 35 mm SLR cameras List of Abbreviations blue B-function ball-function (shutter remains open until release button, formerly a ball, is pressed; see T-function) suitable for eyeglass wearers Camera, Cyan Camera, Auflicht (=incident light)
  • Page 53 Photomicrography with ANNEX 35 mm SLR cameras List of abbreviations Carl Zeiss wrench opening T2-Adapter standard adapter for 35 mm cameras T-function Time function (shutter remains open after first press and closes only after second press, see B-function) maximum exposure time...

  • Page 54: List Of Key Words

    ANNEX Photomicrography with Carl Zeiss List of key words 35 mm SLR cameras List of Key Words Page adjusting aid............................3-6 ambient conditions .......................... 1-10 camera port ............................2-6 CC filter ............................3-12 Changing the fuses ........................... 4-3 color balance........................... 3-11 color distortion ..........................

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