Zeiss Axiocam 105 color Installation & Reference Manual
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Zeiss Axiocam 105 color Installation & Reference Manual

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October 2013

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  • Page 1 October 2013...
  • Page 3 Carl Zeiss or that these are not known to Carl Zeiss due to gross negligence and that furthermore Carl Zeiss has for these reasons refrained from eliminating these errors or inaccuracies appropriately.
  • Page 4 Notice from the Producer The referenced Software of Carl Zeiss ("Zeiss Software"), contained in this documentation, was designed, realized, verified, validated and released in a certificated process environment. The quality management system is certified following DIN EN ISO 9001. The fields of application of the Software are common tasks and applications in microscopy and/or imaging (so called "Off-The-Shelf...
  • Page 5 EU directives.  Please contact your Carl Zeiss sales/service organization for details relating to disposal and recycling.  This product must not be disposed of with domestic waste or using municipal waste disposal services.

  • Page 7: Table Of Contents

    2.3 Adjust Property Page ..............21 Exposure ................. 22 Automatic Exposure ............23 White Balance ................. 23 Color Adjustment ..............24 Histogram ................25 2.4 Frame Property Page ..............27 Camera Mode ................27 Frame ..................28 Axiocam 105 color Installation + Reference Guide...
  • Page 8 Recommended Monitor Settings ..........41 2.8 Background Information .............. 41 Gamma Setting ............... 41 Configurable Toolbars for Operating the Camera ....41 3. Trouble Shooting .................. 42 3.1 Software ..................42 4. Technical Data ..................44 4.1 Axiocam 105 color ............... 44 Printed 10.2013...

  • Page 9: Installation

    Installation 1. Installation The Axiocam 105 color is a high-resolution digital camera with a color CMOS sensor and USB 3.0 interface for universal light-microscopy applications. To make it easier for you to set up the camera, please follow the instructions in these chapters step by step.
  • Page 10 Installation Limitation of liability No warranty shall be assumed by Carl Zeiss during the warranty period if the equipment is operated without observing the safety regulations. In any such case, Carl Zeiss shall be exempt from statutory liability for accidents resulting from such operation.

  • Page 11: General Notes

    (radiators, stoves). Overheating can cause noisy images.  The camera housing can be cleaned using normal microscope cleaning material. In case of repair please contact your local Carl Zeiss representation. Backup Copies We strongly recommend that all users save the data they create, such as images, measurement data, archives, reports, forms and documents, at regular intervals on an external medium.

  • Page 12: System Requirements

    The appropriate system requirements relating to hard- and software equipment can be found in the file "Installation Guide.PDF" on the actual product DVD. Contents of Delivery  Axiocam 105 color  USB 3.0 data cable  PCI express (PCIe x1) Interface card ...

  • Page 13: Setting Up The Camera System

    Molex power connector cable, PCIe interface card, USB 3.0 cable, camera  Switch off your PC and all connected peripherals.  Disconnect the PC and the peripherals from the mains and open the computer case. Axiocam 105 color Installation + Reference Guide...

  • Page 14: Connecting The Camera To The Pc

     Connect the supplied USB 3.0 cable to the Axiocam 105 color. Tighten the screws with a screwdriver until it is finger tight.  Connect the other end of the USB 3.0 cable to the PCIe interface card.

  • Page 15: Mounting The Camera Onto The Microscope

    Installation Power is supplied to the Axiocam 105 color via the interface card. If you switch off the PC, the camera is also switched off. During operation the camera may heat up. This is quite safe. Mounting the camera onto the microscope To mount the camera onto your microscope’s TV port, use a C-mount...

  • Page 16: Checking The Equipment Has Been Set Up Correctly

     The Axiocam 105 color is mounted onto the microscope and is receiving light.  The Axiocam 105 color is connected to the PC via the USB 3.0 data cable. If the LED on the Axiocam 105 color does not turn red now, please check that you have carried out all of the above steps correctly.
  • Page 17 Installation If you have inserted a USB 3.0 PCIe interface card, which was not supplied by Carl Zeiss or is supported by Carl Zeiss, into your computer, you first need to install this card with the help of the documentation enclosed by the manufacturer. Depending on your operating system this installation may not be necessary, as the drivers may already be integrated into the operating system.
  • Page 18 Installation Printed 10.2013...

  • Page 19: Notes On Maintenance

    Click OK and follow the installation wizard. There is no need to activate any special feature during the software installation process. To complete the camera installation, please go to our website to download and run "Installation 01 - Axiocam 105 color": http://www.zeiss.com/axiocam105 Notes on Maintenance Camera Electronic Camera, interface and power supply are maintenance free.

  • Page 20: Important Note On The Use Of C-Mount Objectives

    Important Note on the Use of C-Mount Objectives The camera can be mounted onto microscopes with standard TV-adapters with C-mount connections. Carl Zeiss supplies the camera complete with a pre-mounted infrared barrier filter, which offers the optical advantage for use on microscopes of a lower sensitivity to dust.

  • Page 21: Image Acquisition

    Image Acquisition General The Axiocam 105 color is a high-resolution digital camera for color images. It has been designed to be used in the field of light microscopy for general observation, routine work, and simple applications in which a sufficient amount of light is available.

  • Page 22: The Standard Workflow

    Image Acquisition Note:  The workbar may in fact be open but covered by the work area. If that is the case, simply click on the Workflow tab at the bottom edge of the work area The Standard Workflow Live starts and closes the live window. Live properties opens and closes the window for adjusting the display characteristic curve and for controlling...

  • Page 23: Step By Step To The First Image

     This function is disabled for the Axiocam 105 color. With some of the other cameras, you can select the speed of the live image (Slow, Medium, Fast). Axiocam 105 color Installation + Reference Guide...
  • Page 24 Image Acquisition  Click on the icon Show live window properties to open the live properties. Here you can see the settings for the display of the image like Brightness, Contrast and Gamma. Settings made here are passed on the acquired image.
  • Page 25  Enter further settings for the selected camera on the Adjust, Frame and General property pages.  Click on the camera icon to acquire an individual image, or in the Acquisition menu click on Snap. Axiocam 105 color Installation + Reference Guide...
  • Page 26 Image Acquisition  Click on the Properties icon and activate the Display tab. Any changes you make here are displayed in real time in the image window.  Move the sliders Gamma Contrast Brightness up or down to adjust the image. Good color reproduction on a monitor is achieved with a gamma value of around 0.45.
  • Page 27 Tag. The dialog window Select tagopens where parameters for lettering can be selected.  In the Draw Annotation(s) dialog window a number of different zoom symbols and two buttons to toggle the color channels are available at Axiocam 105 color Installation + Reference Guide...
  • Page 28 Image Acquisition the bottom of the image window  Using the Navigator you can magnify or reduce the image and position the displayed image area in the overview window.  Click on this icon to save the image you have generated to the computer’s hard drive.

  • Page 29: Adjust Property Page

    This property page contains a summary of the most important camera settings, which are constantly used during operation. The property page is split into the fields Exposure, White Balance (with color settings) and Histogram (for the display of an image histogram). Axiocam 105 color Installation + Reference Guide...

  • Page 30: Exposure

    Image Acquisition Exposure The camera’s exposure time can be adjusted to the lighting conditions on the microscope using a slider or by entering an exposure time directly into the input field. When entering the exposure time manually you can state the value range directly using the abbreviations "ms"...

  • Page 31: Automatic Exposure

    Image Acquisition Automatic Exposure The Auto Snap feature is not available for the Axiocam 105 color. If the Auto Live check box is activated, the software continuously sets the exposure during the live image. In this way it is possible to generate images with the correct exposure without having to manually enter an exposure time.

  • Page 32: Color Adjustment

    Image Acquisition  : Using this button you can switch back to the basic LED white balance setting. This setting is optimized for a LED light temperature of 5500 K. Color Adjustment Using the color sliders you can increase or decrease the proportion of each basic color.

  • Page 33: Histogram

    As a result, optimum use is not made of the camera’s resolvable gray value range. You should therefore increase the exposure time or the amount of light in the microscope. Axiocam 105 color Installation + Reference Guide...
  • Page 34 Image Acquisition Log: By clicking on this button it is possible to influence the histogram display. You can toggle between a linear scaling and a logarithmic display of the signals represented in the histogram. The logarithmic display makes it possible to view small amplitudes and high peaks at the same time in a single overview.

  • Page 35: Frame Property Page

    Resolution and Frame. Camera Mode  RGB and B/W: With the Axiocam 105 color, it is possible to use these setting options to select whether you want the camera to produce a color or a black/white image.

  • Page 36: Frame

     With the Axiocam 105 color, three modes are available: in the Color 1x1 mode, the resolution from the camera is set to its highest. In the Color 2x2 and Color 4x4 modes, pixels are combined (in arrays of 2x2 and 4x4 respectively) to represent 1 pixel.
  • Page 37 (TIF, BMP, JPG etc.), this option is not available. With these image formats the image data is converted in accordance with the display characteristic curve that has been set. Axiocam 105 color Installation + Reference Guide...

  • Page 38: General Property Page

    Image Acquisition General Property Page On this property page you enter the basic settings for the camera. Color Saturation The slider for saturation can be used to increase or reduce the intensity of the color impression. The default setting is 0. Printed 10.2013...

  • Page 39: Image Orientation

    Image Orientation An Axiocam 105 color image is adjusted in such a way that the image orientation corresponds to the image in the eyepiece when mounted on an upright microscope with the front of the camera facing you.
  • Page 40 Image Acquisition Shading correction to improve image quality In microscopy, numerous side effects can have an undesirable influence on image quality. Dust and particles in the beam path, for example, can leave dark spots in the image, and, under certain conditions, diaphragms and optics can cause slight shadowing that increases towards the image edge, due to unavoidable physical effects.
  • Page 41 Changes to the settings of other optical components, such as an Optovar, the camera adapter or the condenser, are not taken into account and will make it necessary to repeat the generation of the reference images. Axiocam 105 color Installation + Reference Guide...
  • Page 42 Image Acquisition  If several cameras are operated on a system (e.g. an AxioCam MRm and an AxioCam IC), the reference images for each camera will be managed separately.  In the Multidimensional Acquisition function, a function for fluorescence-channel-specific shading correction is offered under Extended parameters on the C (Channel) property page.

  • Page 43: Practical Notes On Operation In Axiovision

    In the AxioVision menu Acquisition you will find the Status function. If you click on the Reset button in the Status dialog window, the following settings of the active camera are reset: Axiocam 105 color Installation + Reference Guide...
  • Page 44 Image Acquisition Camera parameters for all cameras (as available): Exposure time 20 ms White balance 3200 K Auto Live Sensitivity Range 100% Frame (ROI) Full frame Image Orientation No changes Shading Correction Color Saturation No changes Binning 1x1 (sensor basic resolution) With the function Load Factory Defaults (context menu of the live image) you can reset camera settings in addition to the settings for the live display: Spot Meter / Focus Bar...

  • Page 45: Notes On Optimum Color Reproduction In Axiovision

    Image Acquisition Notes on Optimum Color Reproduction in AxioVision Below you will find a detailed description of how best to set up AxioVision to achieve optimum color reproduction with the Axiocam 105 color. Note:  For an optimum color reproduction the monitor first has to be adjusted.

  • Page 46: Information For Displaying Axiocam Images In Axiovision

    Image Acquisition Information for Displaying Axiocam Images in AxioVision So that intelligent use can be made of this feature, in AxioVision it is possible to influence the display using the characteristic curve. To do this, right-click in the image acquired and select from the context menu the Properties function.
  • Page 47 "handles". Click the Save button to store the current settings. You can use these settings when displaying another image by clicking the Restore button. This enables you to compare different images. Axiocam 105 color Installation + Reference Guide...

  • Page 48: Recommended Camera Settings

    Image Acquisition If you activate the Apply to all T check box the settings in all the acquired instants of exposure are applied to the relevant channel. Note:  You can get the best display of colored objects, if the gamma setting is in the range of approx.

  • Page 49: Recommended Monitor Settings

    The size of the toolbar buttons can also be adjusted, helping you to work quickly with the mouse. A detailed description of how to create toolbars can be found in Chapt. 9 "Configuration" - "Adapting Toolbars" in the AxioVision Manual. Axiocam 105 color Installation + Reference Guide...

  • Page 50: Trouble Shooting

    Software Please also pay attention to all the comments in the file ReadMe.TXT, which is displayed during the driver installation.  Axiocam 105 color does not appear in the menu of selectable cameras  Make sure that the Axiocam 105 color files have been installed in accordance with the installation of the software.
  • Page 51  Try plugging the USB 3.0 interface card into a slot that is not directly next to the Graphics card slot.  Disable "USB selective suspend setting" under Start >Control Panel >Power Options >Change plan settings (for whichever is your active plan) >Change advanced power settings. Axiocam 105 color Installation + Reference Guide...

  • Page 52: Technical Data

    Technical Data 4. Technical Data Axiocam 105 color Including AxioVision LE64, USB 3.0 connecting cable, USB 3.0 PCIe interface card, Power connector cables Sensor type Aptina CMOS color sensor Number of pixels 2560 (H) x 1920 (V) = 5 megapixels Pixel size 2.2 µm x 2.2 µm...
  • Page 53 Technical Data Axiocam 105 color Installation + Reference Guide...

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