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Gilson Manuals
Laboratory Equipment
Pipetman F
Standard operating procedure
Gilson Pipetman F Standard Operating Procedure
Decontamination procedures
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Standard Operating Procedure for Pipettes
Decontamination Procedures
for
Gilson Pipettes
(defined for Users to Incorporate in SOPs)
in Accordance with ISO8655 Standard
Procedure LT802288/C - © 2003 Gilson SAS All rights reserved
October 2003
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Summarization of Contents
Introduction
Decontamination Room
The decontamination room must be separate from all other rooms. Only authorized personnel should enter.
Equipment and Clothing
Lists required equipment like Geiger-Müller counter, benches, safety hood, gloves, and cleaning materials.
Solutions for Decontaminating and Cleaning
Recommends a mixture of detergent and disinfectant for chemical and biological decontamination.
Precautions
Emphasizes risk of contamination, recommends hepatitis B vaccination, and use of protective clothing.
Procedure for End-users
Handling Pipettes with Radioactive Liquids
Check pipettes for radioactivity if used with radioactive liquids. Follow safety protocols if radioactive.
Handling Non-Radioactive Pipettes
For non-radioactive pipettes, proceed with disassembly, decontamination, and drying procedures.
Complete Procedure for Gilson Distributors
Package Radioactivity Check
Scan the outside of the package for radioactivity (beta, gamma, X-rays) using a Geiger-Müller counter.
Pipette Radioactivity Check
Unpack pipettes under a safety hood and check each one individually for radioactivity.
Disassembly Procedures
Pipetman P and F Disassembly
Steps to remove tip-ejector, connecting nut, piston assembly, seal, and O-ring.
Pipetman 8x200 Disassembly
Procedure to unscrew nut, remove screws, lift nozzle assemblies, and remove pistons.
Microman Disassembly
Involves unscrewing capillary-holder and removing clamp assembly and spring.
Distriman Disassembly
Steps to disassemble piston retaining assembly and remove filling knob.
Pipetman Ultra Single Channel Disassembly
Procedure to remove tip-ejector, unscrew tip-holder, and remove piston.
Pipetman Ultra Multichannel Disassembly
Steps to separate head from body and remove components like clips and pistons.
Decontamination Methods
Manual Decontamination Method
Wipe pipette body and parts with Aniospray 41, clean tip-holder, and dry with distilled water.
Immersion Decontamination Method
Immerse parts in an ultrasonic bath with Aniosyme P.L.A. solution, then dry in an oven.
Associated Documents
Pipetman User Guides
List of user guides for various Pipetman models with their respective Gilson references.
Verification Procedure Reference
Reference for the Verification Procedure for Accuracy and Precision.
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