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MODELS 6400/6405
SPECTROPHOTOMETERS
OPERATING MANUAL
640 050/REV B/11-99

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Table of Contents
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Summarization of Contents

Instrument Introduction
Instrument Description
Overview of the instrument's features and capabilities.
Instrument Specifications
Detailed technical specifications of the spectrophotometer.
Instrument Installation
Unpacking Procedure
Procedure for removing the instrument from its packaging.
Power and Voltage Setup
Details on connecting the unit to power and setting voltage.
Instrument Controls
Explanation of the instrument's buttons, keys, and display icons.
Rear Panel Connections
Description of the rear panel connectors and their functions.
Instrument Operation
Operating Overview and System Tests
General overview of functions and power-on system tests.
Instrument Set-up Menu
Configuration options for language, clock, display, and other settings.
Photometrics Mode Operations
How to perform transmittance, absorbance, or concentration measurements.
Spectrum Mode Operations
How to perform wavelength scans and analyze spectral data.
Multi-Wavelength Mode Operations
How to measure at two wavelengths and calculate differences/ratios.
Kinetics Mode Operations
How to perform time-based rate measurements.
Quantitation Mode Operations
How to construct and use calibration curves for concentration analysis.
Good Practice Guidelines
Recommendations for optimal instrument performance and sample handling.
Instrument Maintenance
General Maintenance
Routine cleaning and care instructions for the instrument.
Tungsten Halogen Lamp Replacement
Procedure and safety precautions for replacing the tungsten lamp.
Deuterium Lamp Replacement
Procedure and safety precautions for replacing the deuterium lamp.
Optional Accessories and Spares
Available Accessories
List of available optional accessories for the spectrophotometer.
Spare Parts
List of spare parts, including lamps.
Interfacing and Connectivity
Serial Interface Description
Description of the RS232 interface and its capabilities.
RS232 Connection Pinout
Details of the RS232 connector pin assignments for data transfer.
Recorder Output
Information on analog outputs for recording readings.
Warning and Error Messages
Power On Self Test Messages
Warnings and errors occurring during the instrument's startup tests.
Operation Mode Specific Messages (Part 1)
Errors and warnings specific to different operational modes.
Photometrics Mode Specific Errors
Errors related to concentration factor in photometrics mode.
Spectrum Mode Specific Warnings
Warnings and errors encountered during spectrum scanning.
Multi-Wavelength and Kinetics Mode Messages
Warnings and errors for multi-wavelength and kinetics modes.
Quantitation Mode Specific Warnings
Warnings and errors related to quantitation calibration and data.
Accessories Specific Messages
Messages related to sipper pump accessory operation.
Example Printouts
Photometrics Mode Printout
Example of data printed for photometric measurements.
Spectrum Mode Printout
Example of data printed for spectrum scans.
Multi-Wavelength Mode Printout
Example of data printed for multi-wavelength measurements.
Kinetics Mode Printout
Example of data printed for kinetics measurements.
Quantitation Mode Printout
Example of data printed for quantitation measurements.

Table of Contents