Table of Contents
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BX3-URA
BX3-RFAS
U-LH100HGAPO
U-LH100HG
U-RFL-T
U-AN-2
BX3-25ND6
BX3-25ND25
This instruction manual is for the Olympus Reflected Fluorescence System. To ensure the safety,
obtain optimum performance and to familiarize yourself fully with the use of this system, we
recommend that you study this manual thoroughly before operating the microscope. Retain this
instruction manual in an easily accessible place near the work desk for future reference.
INSTRUCTIONS
REFLECTED
FLUORESCENCE
SYSTEM
A X 7 8 8 8
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Summarization of Contents
USING THE CONTROLS
General Precautions for Observation
General safety and handling precautions before operating the reflected fluorescence system.
Selecting the Fluorescence Mirror Unit
Guidance on choosing the appropriate fluorescence mirror unit based on the fluorochrome used.
Turning the Power Supply Unit On
Steps for safely powering on the illumination system and allowing it to stabilize.
Centering the Field Iris Diaphragm
Procedure to adjust the field iris diaphragm for optimal contrast and reduced stray light.
Centering the Aperture Iris Diaphragm
Steps to center the aperture iris diaphragm for brightness and contrast adjustment.
Centering the Mercury Burner
Instructions for aligning the mercury burner poles for uniform illumination.
Mounting the ND Filters
Process for inserting Neutral Density (ND) filters to reduce specimen color fading.
Attaching the Light Shield Sheet
How to attach a light shield sheet to prevent image degradation from reflected light.
SIMULTANEOUS FLUORESCENCE OBSERVATIONS
Simultaneous Reflected Fluorescence and Phase Contrast Observations
Method for combining reflected fluorescence with phase contrast microscopy.
Simultaneous Reflected Fluorescence and Transmitted Light Differential Interference Contrast (DIC) Observations
Method for combining reflected fluorescence with transmitted light DIC microscopy.
ASSEMBLY
Assembly Diagram
Visual guide illustrating the sequence of module assembly for the system.
Detailed Assembly Procedures
Step-by-step instructions for attaching fluorescence mirror units and other components.
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