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Leica DM4000 B
Leica DM4000 M
Leica DM5000 B
Operating Manual
1

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Summarization of Contents

Safety Notes
General Safety Notes
General safety precautions for device operation.
Electrical Safety
Safety guidelines related to electrical components and power.
Assembling the Microscope
Stage Assembly
Instructions for mounting the microscope stage.
Condenser Installation
Procedures for installing the microscope condenser.
Tube and Eyepieces Installation
Steps for attaching the microscope tube and inserting eyepieces.
Transmitted Light Source Installation
Instructions for installing the transmitted light source lamp housing.
Incident Light Source Installation
Procedures for installing the incident light source lamp housing.
Incident Light Filter Turret Equipping
How to install filter and reflector cubes into the incident light turret.
Polarizer and Analyzer Installation
Instructions for installing transmitted and incident light polarizers/analyzers.
DIC Prisms Insertion
Guide for inserting DIC prisms into the tube slot.
Optional Accessories Overview
Mentions optional accessories like Ergomodule, Mirror Housing.
Power Supply Connection
Steps to connect the microscope stand to the power supply.
CTR5000 Electronics Box Connection
How to connect the CTR5000 electronics box to the microscope.
Startup
Functional Principle
Explains the basic operation and function keys of the microscope.
Switching on the Microscope
Step-by-step guide to powering on the microscope.
The Display and Its Content
Describes the information shown on the microscope's display panel.
The Function Keys
Overview of defined and variable function keys on the microscope stand.
Köhler Illumination Setup
Explanation of the Köhler illumination setup and factory settings.
Bright Field Illumination Procedure
Step-by-step guide for setting up bright field illumination.
Checking Phase Contrast Rings
Procedure for verifying and adjusting phase contrast light rings.
Adjusting the Light Sources
Instructions for adjusting light sources, including centering.
Operation
Switching on the Microscope
Steps to power on the microscope and its components.
Stage and Specimen Movement
How to adjust stage movement and torque.
Focusing Procedure
Instructions for using the focus dials for coarse and fine adjustment.
Tube Adjustments
Information on adjusting viewing distance, angle, and eyepiece extension for different tubes.
Eyepiece Usage
Guidance on using eyepieces, including aperture protectors and vision correction.
Objective Handling
How to manually move objectives into position and how the microscope recognizes them.
Magnification Changer Operation
Details on operating the coded magnification changer.
Light Source Brightness
How to adjust light source brightness using function keys.
Diaphragm Settings
How to change aperture and field diaphragm settings.
Imaging Procedure for Leica DM4000 B/DM5000 B
Bright Field (TL)
Steps for bright field imaging with transmitted light.
Phase Contrast
Procedure for phase contrast imaging with transmitted light.
Dark Field (TL)
Steps for dark field imaging with transmitted light.
Polarization (TL)
Procedure for polarization imaging using transmitted light.
Differential Interference Contrast (TL)
Steps for DIC imaging using transmitted light.
Fluorescence Imaging
Guide to performing fluorescence imaging, including intensity adjustment.
Imaging Procedure for Leica DM4000 M
Bright Field (Incident Light)
Steps for bright field imaging using incident light.
Dark Field (Incident Light)
Procedure for dark field imaging using incident light.
Polarization (Incident Light)
Steps for polarization imaging using incident light.
Interference Contrast (Incident Light)
Guide for interference contrast imaging using incident light.
Bright Field (Transmitted Light)
Steps for bright field imaging using transmitted light.
Trouble Shooting
Stand Issues
Troubleshooting for issues related to the microscope stand and power.
Illumination Problems
Solutions for dark or flickering illumination problems.
Bright Field Troubleshooting
Common issues and remedies for bright field imaging.
Dark Field Troubleshooting
Solutions for problems encountered with dark field imaging.
Phase Contrast Troubleshooting
Remedies for issues preventing phase contrast observation.
Polarization Troubleshooting
Common problems and solutions for polarization imaging.
Fluorescence Troubleshooting
Solutions for dark or weak fluorescence imaging.
Display Troubleshooting
What to do if the display flashes or shows error messages.
Care of the Microscope
Dust Cover Usage
Instructions for using the dust cover to protect the microscope.
General Cleaning Guidelines
General guidelines for cleaning microscope components.
Cleaning Glass Surfaces and Objectives
Procedures for cleaning glass surfaces, lenses, and objectives.
Handling Acids and Bases
Safety precautions for handling chemicals around the microscope.

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