Related Manuals for Leica DM5000 B
Summarization of Contents
Safety Notes
General Safety Notes
Covers general safety guidelines for the microscope, including compliance with safety regulations.
Electrical Safety
Details electrical specifications and safety precautions for the microscope and its power supply units.
Disposal
Provides instructions on the proper disposal of the microscope and its components, complying with regulations.
Unpacking the Microscope
Installation Location
Specifies the recommended environmental conditions for microscope installation and use.
Transport
Details precautions for shipping and transporting the microscope and its accessory components.
Assembling the Microscope
Specimen Stage
Instructions for mounting and securing the specimen stage onto the microscope stand.
Condenser
Step-by-step guide for installing and centering the microscope condenser.
Tube and Eyepieces
Procedures for mounting the microscope tube and inserting eyepieces.
Objectives
Guidance on installing objectives into the objective turret and proper handling.
Light Sources for Transmitted Light
Instructions for installing and connecting lamp housings for transmitted light illumination.
Light Sources for Incident Light
Detailed steps for installing and connecting lamp housings for incident light.
Equipping the Incident Light Turret Disk
Guide on how to insert filter and reflector cubes into the incident light turret disk.
Polarizer and Analyzer
Instructions for installing transmitted and incident light polarizers and analyzers.
DIC Prisms
Steps for inserting objective prism slides and DIC prisms for differential interference contrast.
Optional Accessories
Information on installing optional accessories like ErgoModule, booster lenses, and mirror housings.
Connection to Power Supply
Procedure for connecting the microscope stand to the power supply unit.
Connection to CTR5000 Electronics Box
Instructions for connecting the CTR5000 electronics box for specific microscope models.
Startup
Functional Principle
Explains the basic operational principles and function key usage of the microscope.
Switching on the Unit
Step-by-step guide on how to power on and initialize the microscope.
The Display
Description of the microscope's display screen and its various indicators.
The Function Keys
Details on the fixed and variable function keys and their assignments.
Köhler Illumination
Procedure for setting up Köhler illumination for optimal microscope performance.
Transmitted Light Köhler Illumination
Specific steps for performing Köhler illumination with transmitted light.
Incident Light Köhler Illumination
Specific steps for performing Köhler illumination with incident light.
Checking Phase Contrast Rings
Procedure for checking and centering the phase contrast light rings.
Setting Motorized Polarizers
Instructions for setting motorized polarizers for specific microscope models.
Adjusting Light Sources
Guidance on adjusting and centering the microscope's halogen and discharge lamps.
Operation
Switching on the Unit
Steps for powering on the microscope and its associated units.
Stages and Object Displacement
Details on operating the specimen stage and adjusting movement rates.
Focusing
Instructions for using the focus wheels for coarse and fine adjustment.
Tubes
Information on adjusting microscope tubes, including interpupillary distance and beamsplitting.
Eyepieces
Guidance on using eyepieces, including reticle focusing and vision correction.
Objectives
Instructions for selecting and using objectives, including immersion oil and centering.
Magnification Changer
Details on operating the manual magnification changer and its settings.
HCP 1x/1.6x Tube Optics with Bertrand Lens
Information on HCP tube optics for polarizing microscopy, including Bertrand lens use.
Light Sources
Instructions for adjusting light intensity and brightness for transmitted and incident light.
Aperture and Field Diaphragm
Guidance on adjusting the aperture and field diaphragms for optimal contrast.
Contrast Methods for Leica DM4000 B/DM4500 P/DM5000 B
Transmitted Light Contrast Methods
Covers bright field, phase contrast, dark field, and polarization methods for transmitted light.
Bright Field (TL)
Procedure for setting up and using the bright field contrast method.
Phase Contrast (TL)
Procedure for setting up and using the phase contrast method.
Dark Field (TL)
Procedure for setting up and using the dark field contrast method.
Polarization (TL)
Instructions for setting up and performing polarization microscopy.
Differential Interference Contrast (TL)
Steps for setting up and using differential interference contrast (DIC) methods.
DIC for DM4500 P
Specific instructions for differential interference contrast on the DM4500 P model.
DIC for DM5000 B
Specific instructions for differential interference contrast on the DM5000 B model.
Fluorescence
Guide to using fluorescence microscopy, including filter cubes and intensity adjustment.
Contrast Methods for Leica DM4000 M
Incident Light Contrast Methods
Covers bright field, dark field, polarization, and interference contrast for incident light.
Bright Field (IL)
Procedure for setting up and using the bright field contrast method with incident light.
Dark Field (IL)
Procedure for setting up and using the dark field contrast method with incident light.
Polarization (IL)
Instructions for setting up and performing polarization microscopy with incident light.
Interference Contrast (RL)
Steps for setting up and using interference contrast methods with incident light.
Transmitted Light Contrast Methods
Covers bright field and polarization methods for transmitted light.
Bright Field (TL)
Procedure for setting up and using the bright field contrast method with transmitted light.
Polarization (TL)
Instructions for setting up and performing polarization microscopy with transmitted light.
Troubleshooting
Stand Issues
Common problems related to the microscope stand not responding and their solutions.
Illumination Problems
Troubleshooting for issues like dark images, uneven illumination, and flickering lights.
Bright Field Issues
Solutions for problems encountered in bright field microscopy, such as focus or contrast issues.
Dark Field Issues
Troubleshooting steps for achieving proper dark field contrast and image quality.
Phase Contrast Issues
Solutions for problems that prevent phase contrast observation.
Polarization Issues
Common problems in polarization microscopy and their remedies.
Fluorescence Issues
Troubleshooting steps for fluorescence microscopy, including dark or weak fluorescence.
Display Errors
Resolving issues where the display flashes or shows error messages like 'FAIL!'.
Care of the Microscope
Dust Cover Usage
Guidelines for using the dust cover to protect the microscope and accessories.
Cleaning Procedures
Detailed instructions for cleaning various parts of the microscope, including optics and surfaces.
Handling Acids and Bases
Precautions for working with acids and bases to protect optical and mechanical parts.
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