Shodex GPC KD-800 Series Operation Manual

Shodex GPC KD-800 Series Operation Manual

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(Please read this manual carefully to achieve the best and consistent column performance for a long time)
Important Handling Instructions
Caution!
· Please consult the Safety Data Sheet (SDS) of reagents and solvents used with the column
and understand their proper handling methods to prevent potential health hazards or death
from occurring.
· Please wear appropriate personal protective equipment such as lab goggles and gloves
when handling organic solvents and acid and alkaline reagents. Avoid any direct physical
contact to prevent chemical injuries.
Before Using the Column
(1) Please visually inspect the package and outside of the column for any damage.
(2) Please check if product name and serial number written on the column package, column name tag, and
enclosed Certificate of Analysis (CoA) are matching and correct.
1. Introduction
Thank you for purchasing the Shodex product. Shodex GPC KD-800 series is a size exclusion
chromatography column used with organic solvents. The column series is suitable for the separations of organic
compounds and/or oligomers, and molecular weight distribution analysis of high molecular weight polymers.
The columns with different exclusion limits are available. Please select columns with target molecular weight
ranges (see below) that meet the molecular weight range of your samples.
2. Column Components
3. Column Specifications
Product
Product Name
Code
GPC KD-801
F6028210
GPC KD-802
F6028220
GPC KD-802.5
F6028225
GPC KD-803
F6028230
GPC KD-804
F6028240
GPC KD-805
F6028250
GPC KD-806
F6028260
GPC KD-806M
F6028290
Operation Manual
Shodex
GPC KD-800 series
Column Size
Particle
(mm)
Size
(μm)
I.D.
Length
8.0
300
6
8.0
300
6
8.0
300
6
8.0
300
6
8.0
300
7
8.0
300
10
8.0
300
10
8.0
300
10
SHOWA DENKO K.K. (https://www.shodex.com/)
Theoretical
Target Molecular
Plate Number
Weight Range
(Per Column)
≥ 17,000
100 - 1,500
≥ 17,000
200 - 4,000
≥ 17,000
400 - 10,000
≥ 17,000
1,000 - 50,000
≥ 17,000
4,000 - 200,000
≥ 11,000
30,000 - (4,000,000)
≥ 11,000
30,000 - (40,000,000)
≥ 13,000
1,000 - (40,000,000)
ver.211124E
Exclusion
*1
*1
Limit
2,500
7,000
20,000
70,000
200,000
*2
*2
(4,000,000)
*2
*2
(40,000,000)
*2
(40,000,000)
*2
1

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Summary of Contents for Shodex GPC KD-800 Series

  • Page 1 Certificate of Analysis (CoA) are matching and correct. 1. Introduction Thank you for purchasing the Shodex product. Shodex GPC KD-800 series is a size exclusion chromatography column used with organic solvents. The column series is suitable for the separations of organic compounds and/or oligomers, and molecular weight distribution analysis of high molecular weight polymers.
  • Page 2   30 % o-Chlorophenol/Chloroform   ×     30 % HFIP/Chloroform   × ×   Tetrahydrofuran (THF)  × ×   Chloroform  × × Dimethyl sulfoxide (DMSO)  × × Carbon tetrachloride  SHOWA DENKO K.K. (https://www.shodex.com/)
  • Page 3: Eluent Preparation

    To suppress influences from highly viscos samples, it is recommended to lower their sample solution concentrations. Please use the below table as a reference when preparing sample solutions for molecular weight distribution analyses. SHOWA DENKO K.K. (https://www.shodex.com/)
  • Page 4: Column Installation

    If a KD-806M was used together with columns with different pore sizes, this changes the mixing ratio of the gels and consequently it may deform the linear calibration curve. Caution! · Verify that there is no solvent leak. It may cause electronic leakage, rust, and/or chemical injury. SHOWA DENKO K.K. (https://www.shodex.com/)
  • Page 5: Column Cleaning

    (e.g., trichlorobenzene which has high melting point), replace the in-column solvent with the shipping solvent. Refer to section 7.3 Solvent Exchange for how to replace the eluent. Attention! · Never allow inside of the column to dry. It can damage the column. SHOWA DENKO K.K. (https://www.shodex.com/)
  • Page 6 (2) Do not make a strong impact on the column. Do not drop or hit the column on a hard surface. Refer to the Shodex™ website (https://www.shodex.com/) for product details and their applications. For additional assistance, contact the distributor from whom you purchased the column or contact your regional Shodex...
  • Page 7 11. Warranty (Ver. 4COLUMN) (1) Showa Denko K. K. warrants that the Shodex product, at the time of delivery to the user, will conform to the specification of the attached CERTIFICATE OF ANALYSIS, if the Shodex product is used in accordance with the attached operating manual.

This manual is also suitable for:

Gpc kd-801Gpc kd-802Gpc kd-802.5Gpc kd-803Gpc kd-804Gpc kd-805 ... Show all

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