Shodex HILICpak VT-50 2D Operation Manual

(Please read this operation manual carefully to achieve the best and consistent column performance for a long time.)
Important Handling Instructions
Caution!
· Please consult the Safety Data Sheet (SDS) of reagents and solvents used with the column
and understand their proper handling methods to prevent potential health hazards or death
from occurring.
· Please wear appropriate personal protective equipment such as lab goggles and gloves
when handling organic solvents and acid and alkaline reagents. Avoid any direct physical
contact to prevent chemical injuries.
Before Using the Column
(1) Please visually inspect the column package and the column surface for any damage.
(2) Please check the product name and serial number (Serial no. or S/N) written on the column package and
adhesive label on the column body.
(3) Please download the Certificate of Analysis (CoA) for the purchased product. The CoA can be downloaded
from Shodex website (https://www.shodex.com/download/). You will be asked to enter the serial number.
1. Introduction
Thank you for purchasing the Shodex product. Shodex HILICpak VT-50 2D is a polymer-based hydrophilic
chromatography (HILIC) chlromatography column modified with amino group. It is suitable for separation and
analysis of anion compounds such as phosphorylated sugars and organic acids.
2. Column Components
Please refer to the Shodex website: https://www.shodex.com/en/da/07.html
3. Column Specifications
Product
Product Name
Code
F7630400 HILICpak VT-50 2D
F6711300 HILICpak VT-50G 2A
Base Material : Spherical porous particles of polyvinyl alcohol modified with quaternary amino group
Column Housing : PEEK
Screw Type : Internally-threaded type No.10-32 UNF
Shipping Solvent : 25 mM Formic Acid aq. Solution/Acetonitrile =15/85
4. Usable Conditions
Product Name
HILICpak VT-50 2D
HILICpak VT-50G 2A
Usable solvents are listed below.
(1) A mixture of water and acetonitrile or water and methanol of any ratio can be used. Up to 100 % water,
acetonitrile, and methanol can be used. Use of organic solvents other than acetonitrile or methanol is not
warrantied.
Operation Manual
Shodex HILICpak VT-50 2D
Column Size (mm)
I.D.
2.0
2.0
Flow Rate (mL/min)
Recommended Maximum
0.2 - 0.3
-
Resonac Corporation (https://www.shodex.com/)
Particle Size
(μm)
Length
150 5
10 5
Maximum
Pressure
(MPa/column)
0.5 10
- -
ver.20221202E
Theoretical Plate Number
(Per Column)
≥ 4,500
(Guard Column)
pH Temperature
Range Range (°C)
2 - 13 4 - 60
1