Shodex HILICpak VG-25 4D Operation Manual

(Please read this operation manual carefully to achieve the best and consistent column performance for a long time.)
Important Handling Instructions
Caution!
· Please consult the Safety Data Sheet (SDS) of reagents and solvents used with the column
and understand their proper handling methods to prevent potential health hazards or death
from occurring.
· Please wear appropriate personal protective equipment such as lab goggles and gloves
when handling organic solvents and acid and alkaline reagents. Avoid any direct physical
contact to prevent chemical injuries.
Before Using the Column
(1) Please visually inspect the column package and the column surface for any damage.
(2) Please check the product name and serial number (Serial no. or S/N) written on the column package and
adhesive label on the column body.
(3) Please download the Certificate of Analysis (CoA) for the purchased product. The CoA can be downloaded
from Shodex website (https://www.shodex.com/download/). You will be asked to enter the serial number.
1. Introduction
Thank you for purchasing the Shodex product. Shodex HILICpak VG-25 series is polymer-based hydrophilic
chromatography (HILIC) chlromatography columns modified with amino group. It is suitable for separation and
analysis of sugars and sugar alcohols.
2. Column Components
Please refer to the Shodex website: https://www.shodex.com/en/da/07.html
3. Column Specifications
Product
Product Name
Code
F7631000 HILICpak VG-25 4D
F6711800 HILICpak VG-25G 4A
Base Material : Spherical porous particles of polyvinyl alcohol modified with amino group
Column Housing : PEEK
Screw Type : Internally-threaded type No.10-32 UNF
Shipping Solvent : Water/Acetonitrile = 20/80
4. Usable Conditions
Product Name
HILICpak VG-25 4D
HILICpak VG-25G 4A
Usable solvents are listed below.
(1) A mixture of water and acetonitrile or water and methanol of any ratio can be used. Up to 100 % water,
acetonitrile, and methanol can be used. Use of organic solvents other than acetonitrile or methanol is not
warrantied.
Operation Manual
Shodex HILICpak VG-25 4D
Column Size (mm)
I.D.
4.6
4.6
Flow Rate (mL/min)
Recommended Maximum
0.3 - 0.8
-
Resonac Corporation (https://www.shodex.com/)
Particle Size
(μm)
Length
150 2.5
10 2.5
Maximum
Pressure
(MPa/column)
1.0 15
- -
ver.20250116E
Theoretical Plate Number
(Per Column)
≥ 16,500
(Guard Column)
pH Temperature
Range Range (°C)
2 - 13 4 - 60
1