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Summary of Contents for Labconco Auto Densi-Flow
- Page 1 Auto Densi-Flow® Model 4517000 – 115V Model 4517200 – 230V INSTRUCTION MANUAL Product designs are subject to change without notice © 1998 Labconco Corporation 0114520, Rev. C / ECO 9467 Printed in U.S.A.
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Page 3: Table Of Contents
General Description Component Identification Probe Setting Pump Assembly & Operating Controls Installation Pump Level Adjustment Probe Normal Operation Pump Removal of the Gradient Removal of the Specific Bands Layering the Gradient Maintenance Specifications Warranty & Contacting Labconco Corporation Shipping Claims... -
Page 4: Preface
PREFACE Thank you for displaying confidence in us by selecting a Labconco Auto Densi-Flow®. Our design engineers, assemblers and inspectors have utilized their skills and years of experience to ensure that the new Labconco Auto Densi-Flow meets our high standards of quality and performance. -
Page 5: Introduction
As shipped, the carton should contain the following: Catalog No. Description Qty. 4517000 Auto Densi-Flow, 115V model 4517200 Auto Densi-Flow, 230V model 5930001 5/64" Allen Wrench 5310020 Fuse 1 Amp 3 AB SB, 115 model... -
Page 6: General Description
INTRODUCTION General Description The Labconco Auto Densi-Flow is a single apparatus for both depositing a preformed gradient smoothly and continuously and gently removing centrifuged sample layers. The instrument obtains a high degree of resolution and can deposit or remove samples within 0.1 mm (0.004 inch) of the gradient surface. -
Page 7: Component Identification
A nylon strip is placed over the stainless steel rollers and is clipped in position at the block on the front edge of the top of the Auto Densi-Flow. This nylon strip should not be tight. It should bow out on the output side of the pumping head. -
Page 8: Probe Setting
INTRODUCTION Figure 1 Probe Setting... -
Page 9: Pump Assembly & Operating Controls
INTRODUCTION 1. Pump Assembly 10. Power Switch 2. Probe 9. Pump Direction 3. Delrin Probe Holder Switch 4. Override Button 8. Pump Speed Control 5. Function Switch 7. Universal Centering Jaws 6. Leveling Feet 6. Spirit Level Figure 2 Pump Assembly and Operating Controls... -
Page 10: Installation
INSTALLATION Installation Pump 1. Remove the pressure bar. 2. Loop the tubing over the rollers with the nylon strip between the roller and the tubing. 3. Replace the pressure bar and tighten it slightly with the swivel clamp. 4. Loop the tubing around the 2 pins located in the semi-circular cutouts in the block retainer located in front of the pumping head. -
Page 11: Normal Operation
NORMAL OPERATION Pump 1. Immerse the inlet end of the tubing (if depositing) or the end of the probe (for removal) into a suitable liquid. 2. Set the speed control to #9 and turn the switch to forward or reverse, as required. 3. -
Page 12: Layering The Gradient
NORMAL OPERATION probe is placed just below the desired band. A syringe connected to the probe through a short length of tubing will usually be more useful for withdrawing the band than the pump. If more than one band has to be withdrawn, start with the uppermost band. After it is taken out, depress the override button to the next band, and so forth until all the bands of interest have been drawn out. -
Page 13: Maintenance
MAINTENANCE Care and Maintenance After completion of a run, rinse the probe with distilled water with the pump to prevent sucrose or any other material from crystallizing or fouling the probe and connecting tubing. Special care is required after use with cesium and other salt gradients because they can permanently damage the probe. -
Page 14: Specifications
SPECIFICATIONS Specifications Resolution: 0.1 mm (0.004 inches) Withdrawal (or deposit) rate: Controlled by pump and tube size from 1 to 500 ml/hr. Probe Materials: Stainless Steel and Teflon®*. Range of Centrifuge Tube Diameters: 10 mm to 35 mm Housing: Anodized and painted aluminum. Dimensions: 12"... -
Page 15: Warranty & Contacting Labconco Corporation
The warranty for all Labconco products will expire one year from date of installation or two years from date of shipment from Labconco, whichever is sooner, except the following: •... -
Page 16: Shipping Claims
NOTE: Do not return goods. Goods returned without prior authorization will not be accepted. Labconco Corporation and its dealers are not responsible for shipping damage. Claims must be filed directly with the freight carrier by the recipient. If authorization has been received to return this product, by accepting this approval, the user assumes all responsibility and liability for biological and chemical decontamination and cleansing.
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