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DMT MYOGRAPH SYSTEM 120CP User Manual

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MYOGRAPH SYSTEM – 120CP
U S E R M A N U A L V O L . 3 . 6

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Summary of Contents for DMT MYOGRAPH SYSTEM 120CP

  • Page 1 MYOGRAPH SYSTEM – 120CP U S E R M A N U A L V O L . 3 . 6...

  • Page 2: Table Of Contents

    CONTENTS SAFETY CHAPTER 1 - SYSTEM OVERVIEW 1.1 INTERFACE 1.2 IMAGING PRESSURE MYOGRAPH CHAPTER 2 – SETTING UP THE MYOGRAPH 2.1 SETTING UP THE COMPLETE IMAGING PRESSURE MYOGRAPH SYSTEM 2.2 SETTING UP STEP-BY-STEP 2.3 THE FIRST FORCE AND PRESSURE CALIBRATIONS 2.4 EXTERNAL PRESSURE MYOGRAPH CONNECTIONS CHAPTER 3 - PRESSURE INTERFACE MENUS 3.1 GENERAL DESCRIPTION OF HOW TO NAVIGATE THE TOUCH SCREEN...

  • Page 3: Safety

    SAFETY The Imaging Pressure Myograph System has been designed for use only in teaching and research applications. It is not intended for clinical or critical life-care use and should never be used for these purposes. Nor for the prevention, diagnosis, curing, treatment, or alleviation of disease, injury, or handicap.

  • Page 4: Ec Declaration Of Conformity

    EC DECLARATION OF CONFORMITY DMT A/S Certify and declare that the following apparatus: Imaging Pressure Myograph System – DMT120CP Restrictive use: Only for laboratory use. Manufactured by: DMT A/S Rho 14 DK-8382 Hinnerup Denmark 2014/30/EU Conforms with the essential requirements of the EMC Directive...

  • Page 5: Chapter 1 - System Overview

    CHAPTER 1 - SYSTEM OVERVIEW 1.1 INTERFACE Touch display Power indicator 2x500ml Schott Duran bottles Figure 1.1 Pressure Interface - front...
  • Page 6 ON/OFF SWITCH Ground connection Flowmeter Inlet for 24 DC port for power oxygen/garbogen to pressure bottles (max 1bar) Connection port for USB connection port Connection port for pH Myograph chamber for computer electrode (optional) Analogue recorder output - connection to external data acquisition Figure 1.2 Pressure Interface - rear...

  • Page 7: Imaging Pressure Myograph

    1.2 IMAGING PRESSURE MYOGRAPH Figure 1.3 Imaging Pressure Myograph – 120CP with close-up detail of the chamber...

  • Page 8: Chapter 2 - Setting Up The Myograph

    CHAPTER 2 – SETTING UP THE MYOGRAPH 2.1 SETTING UP THE COMPLETE IMAGING PRESSURE MYOGRAPH SYSTEM Figure 2.1 Imaging Pressure Myograph – 120CP with close-up detail of the chamber...
  • Page 9 The Imaging Pressure Myograph System will also work with a range of different inverted microscopes for dimensional studies. The DMT Microscope is a standard inverted microscope with a built-in digital CCD USB camera. The DMT Microscope is very effective and recommended in setups requiring standard image analysis.

  • Page 10: Setting Up Step-By-Step

    2.2 SETTING UP STEP-BY-STEP This section describes how to connect the cables in the Imaging Pressure Myograph System as illustrated in figure 2.1. NOTE: BEFORE PROCEEDING SETTING UP STEP-BY STEP MAKE SURE THAT THE PRESSURE INTERFACE AND THE COMPUTER ARE SWITCHED OFF 1.
  • Page 11 4. Microscope/camera to computer connection: (optional for dimensional analysis) A. DMT Inverted Microscope: Inside the DMT Microscope there is a USB camera wired to a USB cable and one USB cable to power the microscope light. Connect the two USB cables from the DMT Microscope to the computer USB ports.

  • Page 12: The First Force And Pressure Calibrations

    However, in order to ensure that the Imaging Pressure Myograph System is working at highest performance, DMT recommends that new force and pressure calibrations are performed before the first use. The force and pressure calibration procedures are described in detail in chapter 3.7.
  • Page 13 Perfusion inlet P1 Perfusion outlet P2 Figure 2.2 External connections for Pressure Myograph 120CP Extra perfusion inlet Figure 2.3 Pressure Interface tube connections with the Pressure Myograph- 120CP...
  • Page 14 NOTE: TO FILL THE TUBING AND AVOID AIR BUBBLES, MOUNT THE CALIBRATION SHUNT (RESISTANCE TUBE) AS SHOWN BELOW AND DESCRIBED IN CHAPTER 3.7.2.1. Calibration shunt tube replacing the silicone tubes to bypass the chamber (delivered in a small box with the system) Figure 2.4 Mounting of the Calibration Shunt tube on the 120CP...
  • Page 15 Fill the P1 Schott bottle with a maximum of 250ml of buffer. In the pressure interface menu, set the pressure P1 and P2 as follow: P1 = 150mm Hg P2 = 70mm Hg Set the Pressure ON Set Flow ON IMPORTANT: •...
  • Page 16 Detach the silicone from the short steel pipe here and attach a 5ml syringe with an 18-gauge needle 4. Attach a 5ml syringe with an 18-gauge needle to the short silicone tubing on the P1 glass cannula and use the syringe to drag buffer into the P1 glass cannula and silicone tube. When all air is removed, re-connect the short silicone tube to the steel pipe on the chamber again.
  • Page 17 7. Inspect the glass cannulas and the silicone tubing in the chamber for air bubbles using a dissection microscope. If no air bubbles are visible, then continue with the mounting of the artery (See USER GUIDE). If not, then continue with step 8-17. 8.
  • Page 18 NOTE: REMEMBER TO CONNECT THE CHAMBER TO THE PRESSURE INTERFACE TO MONITOR P1 AND P2 PRESSURE DURING THIS STEP. 11. Attach a 5-10ml syringe at the extra P2 perfusion Inlet and push a small volume of buffer through the 3-way valve to remove air in the valve. Then close the 3-way valve toward the P2 buffer flask as shown below.
  • Page 19 Creating a superfusion flow in the Imaging Pressure Myograph chamber requires an external peristaltic pump, which is not part of the basic Imaging Pressure Myograph System. Contact DMT for further information about recommended perfusion pump models, specifications, and prices. The superfusion...
  • Page 20 FlowMeter as soon as it is connected to the Pressure Interface and will be ready for use immediately. The External DMT 162FM Flowmeter is connected at the P2 side of the 120CP chamber using the applied silicone tubing with the internal diameter of 1mm. Connect the P2 outlet from the 120CP chamber with the DMT 162FM Flowmeter Inlet and then connect the DMT 162FM Flowmeter outlet with the P2 connection on the Pressure Interface (see in figure below).
  • Page 21 2.4.6 CONNECTING A SUCTION DEVICE Connect the “large” pipe on the Imaging Pressure Myograph chamber cover (see figure 2.8) to a vacuum pump via a suction bottle and a vacuum valve as illustrated in figure 2.1. The internal diameter of the silicone tube used for this connection must be 2mm. (2mm tubing is not included with the Imaging Pressure Myograph System).

  • Page 22: Chapter 3 - Pressure Interface Menus

    CHAPTER 3 - PRESSURE INTERFACE MENUS This chapter contains a detailed description of how to navigate the touch screen menus and how to use the special features of the Pressure Interface. 3.1 GENERAL DESCRIPTION OF HOW TO NAVIGATE THE TOUCH SCREEN Menus on the Pressure Interface are all accessible by a touch screen.

  • Page 23: Power-Up Screen

    3.2 POWER-UP SCREEN After turning on the Pressure Interface, an introduction screen appears. It displays the software version number. While this screen is displayed the system is auto-initializing. After a few seconds, the Main Menu display will appear. The firmware version is shown on the Introduction screen – software revision.

  • Page 24: Main Menu

    MAIN MENU The Main Menu gives a good overall picture of how the Pressure Interface is working. It displays values for the input pressure, output pressure, force, pH, probe temperature and time, and the status of systems including the time, heat, flow and pressure. See next page for a description of the Main Menu. Four sub-menus are accessible from the Main Menu screen: Pressure Menu Heat Menu, Timer and Buzzer Menu Setting Menu...
  • Page 25 Buzzer icon: This icon indicates the status on the buzzer. If the icon is grey the buzzer is OFF. If the icon is green, the buzzer is ON (active) and will make a sound when the timer reaches zero. Timer: 00:00:00 The Timer is a countdown timer that can be set to maximum 24 hours.

  • Page 26: Pressure Menu

    3.4 PRESSURE MENU The pressure is controlled from this menu. Use SELECT to select the line to be changed. When selected the line changes to blue (edit mode). Use DEFAULT or UP and DOWN arrows to change the setpoint value. Pressing ENTER will save the new setpoint.
  • Page 27 The pressure regulator and the flow are also controlled from this menu. To turn the pressure regulator on, press ON outside the line for Pressure. When activated it turns green. In Main Menu the pressure icon also turns green when pressure regulation is on.

  • Page 28: Heat Menu

    3.5 HEAT MENU The chamber and bottle heating temperature are controlled from this menu. To turn the heat on, or to change the temperature for the system, press the HEAT in the Main Menu. The display will enter the Heat Menu and allow the user to change the system temperature, as well as turn heat on or off.

  • Page 29: Timer And Buzzer Menu

    3.6 TIMER AND BUZZER MENU The timer and buzzer are controlled from this menu. Use SELECT to program the timer. The timer can be programmed in intervals from 0 to 23:59:59 (Hours: minutes: seconds). When Set Timer is selected press the UP and DOWN arrows to program the timer.

  • Page 30: Setting Menu

    Prior to shipping, the Imaging Pressure Myograph System has gone through two days of continuous testing, including final force calibrations. However, DMT recommends that new force calibrations are performed before starting to use the Imaging Pressure Myograph System for the first time. The calibration procedure needs to be performed to calibrate the system.
  • Page 31 Enter Force Calibration Menu by pressing Settings in the Main Menu and then press Force Calibration Menu to begin the transducer calibration. The display will show the force calibration procedure. The calibration procedure is listed in 6 individual steps and needs to be performed one at a time to calibrate the force transducer.
  • Page 32 Figure 3.1 Illustration of how to fit the T-balance just behind the P2 glass cannula arm...
  • Page 33 Step 3 - This step initiates the heating process for the chamber. In order for the calibration to be accurate, the transducer must be heated to the same temperature as used in your experiments. This allows for heat-induced expansion of the electronic parts in the transducer.
  • Page 34 Step 6 - This step verifies that the calibration was performed correctly. The Force Chamber reading should be 9.81 ±0.1mN. If the Force Chamber reading is off by more than 0.1mN then remove the weight, press BACK to return to step 4, and repeat the calibration process.
  • Page 35 PRESSURE CALIBRATION PROCEDURE FOR IMAGING PRESSURE MYOGRAPH 120CP 1. Connect the Pressure Interface with the Imaging Pressure Myograph. 2. Connect the Big Ben Pressure Manometer to the 3-way valve at P1 on the Imaging Pressure Myograph as illustrated in figure 3.2. 3.
  • Page 36 6. Close the inlet from the Pressure Interface P1 by turning the handle on the 3-way valve towards the P1 bottle, see figure 3.4C. The inlet to P1 now comes from the pressure manometer and the Imaging Pressure Myograph system is now ready for a calibration procedure. Figure 3.4 Three-way valve flow settings at P1 3.7.2.2 Set-up the pressure calibration on the Pressure Interface for the 120CP System...
  • Page 37 Step 2 - Apply a pressure of 50mmHg to the Imaging Pressure Myograph using the Big Ben Pressure Manometer. When the 3 blue lines of relative values stabilize, go to NEXT STEP. The relative values should be between 3300 to 3900 Step 3 - Apply a pressure of 125mmHg to the Imaging PRESSURE CALIBRATION Pressure Myograph using the Big Ben Pressure Manometer.
  • Page 38 DMT to unlock the pH module. By ordering the pH sensor from DMT, the pH sensor is delivered with the unlocking code and a quick guide how to do it. Entering the code will open the pH Calibration menu.
  • Page 39 If Manual is selected, the manual temperature is used in the pH calibration formula, and the temperature probe is de- activated. In the Manual mode, the temperature of the calibration buffers is measured with a thermometer and entered manually in the Manual Temp.
  • Page 40 Step 3 - Place the pH electrode and temperature probe in the low pH CALIBRATION buffer solution (here pH 4) and turn on stirring of the low buffer solution. When the relative pH output in the blue line is stable, Step no.: go to NEXT STEP.
  • Page 41 SELECT ANALOG OUTPUT (OPTIONAL) 3.7.4 The Analog Output Menu determines which data is sent to the BNC analog outputs (REC 1, REC 2 REC 3, REC 4) on SETTINGS the back of the Pressure Interface, four in total. Each output is individually programmable.
  • Page 42 3.7.5. INTERFACE SETTINGS The Interface Settings sub-menu in Settings has an additional three sub-menus which include: • Temperature Difference • pH Set-up • Factory Diagnostics 3.7.5.1 Temperature Difference (offset) The temperature difference function allows the user to fine- tune the temperature setpoint of the system. Although the temperature setpoint for the system can be set in the Heat Menu, the actual temperature for the system may not heat to the exact setpoint.
  • Page 43 3.7.5.3 Factory Diagnostics LOGIN CODE TO DIAGNOSTICS Entering Factory Diagnostics will display the Login code to Type login code to get acces diagnostics window. This window is for trained technicians and is used for diagnostics and troubleshooting purposes. The general user will not have access to this window. However, entering the proper five-digit login code, will give the trained technician access to diagnostics panels that will Code init value.
  • Page 44 Danish Myo Technology A/S DMT-USA, Inc. E-mail: sales@dmt.dk E-mail: sales@dmt-usa.com Tel.: +45 87 41 11 00 Tel.: +1 734 707 0250 Fax: +45 87 41 11 01 Fax: +1 678 302 7013 DMT-Asia Pacific DMT-China Tel.: +61 2 8814 1597 Tel.: +86 21 6486 9685...

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