DMT 750 TOBS User Manual
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DMT 750 TOBS User Manual

Tissue organ bath system
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USER GUIDE, VOL.2.0
TISSUE ORGAN BATH SYSTEM - 750TOBS

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Summary of Contents for DMT 750 TOBS

  • Page 1 USER GUIDE, VOL.2.0 TISSUE ORGAN BATH SYSTEM - 750TOBS...

  • Page 3: Table Of Contents

    CONTENTS CHAPTER 1 - SYSTEM OVERVIEW 1.1 Tissue Organ Bath System - 750 TOBS: Front Panel 1.2 Tissue Organ Bath System - 750 TOBS: Rear Panel 1.3 Tissue Organ Bath System - 750 TOBS: Chamber CHAPTER 2: SETTING UP THE TISSUE ORGAN BATH 2.1 Tissue chamber handling procedure...

  • Page 4: Chapter 1 - System Overview

    CHAPTER 1 - SYSTEM OVERVIEW 1.1 TISSUE ORGAN BATH SYSTEM - 750 TOBS: FRONT PANEL Mounted to the lid inside the bottles: A constant flow shunt or a fill/empty shunt Micrometer positioner Transducer Perfusion buffer inlet Front door Tissue chamber...

  • Page 5: Tissue Organ Bath System - 750 Tobs: Rear Panel

    1.2 TISSUE ORGAN BATH SYSTEM - 750 TOBS: REAR PANEL 115-230V/50-60Hz Communication indicator automatic voltage selector USB connection ON/OFF switch Analogue recorder output Power connector Suction connection for vacuum pump Oxygen/ Carbogen Pressure inlet, max 10bar/145psi...

  • Page 6: Tissue Organ Bath System - 750 Tobs: Chamber

    1.3 TISSUE ORGAN BATH SYSTEM - 750 TOBS: CHAMBER Chamber detector Tissue chamber Air heating Sample holder (lower part shown) Figure A: 20ml Tissue chamber NOTE: The Chamber Detector registers when the tissue chamber is placed. If the chamber is placed correctly the blue indicator lamp will be lit and the tissue organ bath will work.
  • Page 7 Knob for dismounting chamber disc and temperature probe Glass indicator: On: Glass mounted Oxygenation/ CO Off: Glass removed regulator Temperature Probe Oxygenation/ Carbogen airpipe tubing Perfusion Buffer inlet Drainage tube NOTE: Regarding the oxygenation/CO regulator: the regulators need to be greased AT LEAST twice a year.

  • Page 8: Chapter 2: Setting Up The Tissue Organ Bath

    CHAPTER 2: SETTING UP THE TISSUE ORGAN BATH 2.1 TISSUE CHAMBER HANDLING PROCEDURE The following is a description of how to handle the tissue chambers and the different kinds of sample holders available. 1: Move the transducer 2: Open the chamber and mounting supports/ door.

  • Page 9: Adjusting The Transducer

    2.2 ADJUSTING THE TRANSDUCER When tissue is placed in the sampleholder do a course adjustment on the tissue holder to fit the tissue just between the holder and the transducer without stretching it. With the tissue submerged in the buffer set the force to 0.

  • Page 10: Different Types Of Tissue Sample Holders

    Figure D: Triangular hooks w/stimulation Figure E: Triangular hooks w/circular electrodes clips (optional) stimulation electrodes (optional) NOTE: The above mounting supports are only examples of supports delivered by DMT. If other mounting supports are needed ask your DMT sales representative.

  • Page 11: Isometric Transducer

    750 TOBS system. As part of the general maintenance of the 750 TOBS system, DMT also recommends that the bath is weight calibrated at least once every month, every time the system has been moved or has not been used over a long period.

  • Page 12: Chapter 3: Cleaning And Maintenance

    The 750TOBS is a very delicate and sophisticated piece of research equipment. In order to keep it working at its best, DMT recommend that the following sections are read carefully and that the instructions are followed at all times. DMT strongly recommends that the Tissue Organ Bath and surroundings should be cleaned after each experiment.
  • Page 13 7. Carefully dismount the tissue bath chamber and perform a normal glass wash. 8. If aggressive solution or drugs have been used it is possible to disconnect the temperature probe (see figure 3.1 below) and take out the chamber disc. Clean both with double distilled water, and dry with a paper towel.

  • Page 14: Appendix 1 - Buffer Recipes

    APPENDIX 1 - BUFFER RECIPES PHYSIOLOGICAL SALINE SOLUTION (PSS) 1x PSS (1000ml): Solution 1: Chemical MW (g/mol) Conc. (mmol/l) Conc. (g/l) NaCl 58.44 118.99 6.954 74.56 4.69 0.350 MgSO 246.48 1.17 0.289 KH2PO4 136.09 1.18 0.161 Solution 2: Chemical MW (g/mol) Conc.
  • Page 15 25x Concentrated PSS (1000ml) Solution 1: Chemical MW (g/mol) Conc. (mmol/l) Conc. (g/l) NaCl 58.44 118.99 173.850 74.56 4.69 8.750 CaCl 147.02 2.50 9.200 Solution 2: Chemical MW (g/mol) Conc. (mmol/l) Conc. (g/l) MgSO 246.48 1.17 7.225 KH2PO4 136.09 1.18 4.025 Solution 3: Chemical...
  • Page 16 A.4.1.3 KPSS 1 x KPSS (1000ml): Solution 1 Chemical MW (g/mol) Conc. (mmol/l) Conc. (g/l) 74.56 123.70 9.223 MgSO 246.48 1.17 0.289 136.09 1.18 0.161 Solution 2 Chemical MW (g/mol) Conc. (mmol/l) Conc. (g/l) CaCl 147.02 2.50 0.368 Solution 3 Chemical MW (g/mol) Conc.
  • Page 17 25 x KPSS (1000ml) Solution 1: Chemical MW (g/mol) Conc. (mmol/l) Conc. (g/l) 74.56 123.70 230.575 CaCl 147.02 2.50 9.200 Solution 2: Chemical MW (g/mol) Conc. (mmol/l) Conc. (g/l) MgSO 246.48 1.17 7.225 136.09 1.18 4.025 Solution 3: Chemical MW (g/mol) Conc.
  • Page 18 Danish Myo Technology A/S DMT-USA,Inc. E-mail: sales@dmt.dk E-mail: sales@dmt-usa.com Tel.: +45 87 41 11 00 Tel.: +1 734 707 0250 Fax: +45 87 41 11 01 Fax: +1 678 302 7013 DMT-China DMT-Asia Pacific Tel.: 86-21-6486 9685 Tel.: +61 2 8814 1597...

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