DMT 820MS User Manual
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DMT 820MS User Manual

Muscle strip myograph system
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USER MANUAL, VOL.2.0
MUSCLE STRIP MYOGRAPH SYSTEM - 820MS

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Summary of Contents for DMT 820MS

  • Page 1 USER MANUAL, VOL.2.0 MUSCLE STRIP MYOGRAPH SYSTEM - 820MS...
  • Page 2 MUSCLE STRIP MYOGRAPH SYSTEM MODEL 820MS MUSCLE STRIP MYOGRAPH SYSTEM - MODEL 820MS USER MANUAL...

  • Page 3: Trademarks

    Changes may have been made to the software and hardware it describes since then. New information may be supplied separately. This documentation is provided with the DMT Muscle Strip Myograph System – Model 820MS Document Number: 820MS – UG2.2A All rights reserved. No part of this manual may be reproduced or transmitted in any form or by any means without the written permission of Danish Myo Technology A/S.

  • Page 4: Table Of Contents

    4.3 Force Transducer Replacement ..............................26 4.4 Myograph Maintenance ................................. 28 4.5 Cleaning the myograph .................................. 29 Chapter 5 - The mounting supports and mounting in the 820MS chamber ......................30 Appendix 1 - System specifications ...................................... 31 MUSCLE STRIP MYOGRAPH SYSTEM - MODEL 820MS...

  • Page 5: Introduction

    The Muscle Strip Myograph System - 820MS represents the first state-of-the-art 4-channel myograph system for muscle strips of up to 15 mm in length. DMT developed this system for simultaneous measurement of up to 4 isolated muscle preparations, mounted as strips, between clamp supports. Several types of mounting supports exist, depending on the user’s preferences.

  • Page 6: Safety

    SAFETY The Muscle Strip Myograph System - 820MS has been designed for use in teaching and research only. It is not intended for clinical or critical life-care use and should never be used for these purposes. The 820MS also should not be used for the prevention, diagnosis, curing, treatment, or alleviation of disease, injury, or handicap.

  • Page 7: Emc / Emi

    EMC / EMI This equipment has been tested and found to comply with the limits for a Class B Digital device, pursuant to part 15 of the FCC rules. These limits are designed to provide reasonable protection against harmful interference in residential installations. This equipment generates, uses and can radiate radio frequency energy and, if not installed and used in accordance with the instructions, may cause harmful interference to radio communications.

  • Page 8: Certificate Of Conformity

    CERTIFICATE OF CONFORMITY DMT A/S, Skejbyparken 152, 8200 Aarhus N., Denmark, hereby declares its responsibility that the following product: Muscle Strip Myograph System Model 820MS is covered by this certificate and marked with CE-label conforms with the following standards: EN 61010-1:2001 Safety requirements for electrical equipment for measurement, con- EN 61010-1/Corr.1:2003...

  • Page 9: About This Manual

    Chapter 1 provides an overview of the construction and basic features of the Myo-Interface and the myograph unit. Chapter 2 describes step-by-step how to set-up the complete Muscle Strip Myograph System – 820MS including accessories. Chapter 3 is a complete manual to the Myo-Interface. The chapter describes in detail the construction of the menu system and how to use all the features of the Muscle Strip Myograph System - 820MS.

  • Page 10: Unpacking The Myograph System

    UNPACKING THE MYOGRAPH SYSTEM Take a few minutes to carefully inspect your new Muscle Strip Myograph System - 820MS for damage which may have occurred during handling and shipping. If you suspect any kind of damage, please contact DMT immediately and the matter will be pursued soon as possible.

  • Page 11: Chapter 1 - System Overview

    1.1 Myo-Interface front panel Valve buttons Myo-Interface display Heat indicator Power indicator Figure 1.1 - Interface Front Panel with 820MS chambers 1.2 Interface Rear Panel 4 Transducer connections Gas regulator (needle valve) RS 232 Port for serial connection to PC...

  • Page 12: Muscle Strip Myograph Unit

    Connection to Myo-Interface Micrometer Figure 1.3 Muscle Strip myograph unit Myograph clamp support connected to micrometer Force transducer pin Myograph clamp support connected to force transducer Figure 1.4 Close up of myograph unit MUSCLE STRIP MYOGRAPH SYSTEM - MODEL 820MS USER MANUAL...

  • Page 13: Chapter 2 - Setting-Up

    Figure 2.1 The complete Muscle Strip Myograph System - 820MS 2.2 Setting up step-by-step This chapter contains a complete step-by-step description of how to set up a complete Muscle Strip Myograph System - 820MS as illustrated in figure 2.1 above.

  • Page 14: The First Weight Calibration

    2.3 The first weight calibration Prior to the shipment of the Muscle Strip Myograph - 820MS it has gone through two days of continuous testing including a final weight calibration. However in order to ensure that the myograph is working at highest performance, DMT recommends that a new weight calibration is performed before starting to use the myograph system.

  • Page 15: Chapter 3 - The Interface Menus

    Chapter 3 is a complete manual for the 820MS Interface. This chapter contains a detailed description of how to navigate the touch-screen menus and how to use the special features of the 820MS myograph. The 820MS system can be used in 2 different modes.

  • Page 16: Interface Menus - Calibrated Mode

    BNC outputs on the rear of the interface. The total voltage output from the BNC connectors is 2.5 volts. Power-Up Screen After turning on the 820MS Interface, an “Introduction” screen appears. The system is auto-calibrating the A/D converters while this screen is dis- played.
  • Page 17 Zero Menu This menu is used to zero the output of the transducers. When using a data acquisition program like LabChart by AD Instruments ®, using this feature will reset the baseline of the chart traces without affecting the calibrations or physically changing any pre-load tensions placed on the mounted ves- sels.
  • Page 18 The pin should be as close as possible to the clamp support or mounting pin without touching in order to get the most accurate calibration. Press “NEXT STEP” when the calibration kit has been properly placed. MUSCLE STRIP MYOGRAPH SYSTEM - MODEL 820MS USER MANUAL...
  • Page 19 Step 3 Initiates the heating process for the chambers. In order to perform an accurate calibration the transducers must be heated to the desired ex- perimental temperature to accommodate heat-induced expansion of the electronic parts in the transducer. Otherwise inaccurate readings and trans- ducer drift may occur.
  • Page 20 NOTE: ANYTIME FORCE REC. OUT IS CHANGED, A NEW WEIGHT CALIBRATION OF THE TRANSDUCERS SHOULD BE PERFORMED, ENTERING THE NEW VOLTAGE VALUES INTO THE DATA ACQUISITION SYSTEM BEING USED. MUSCLE STRIP MYOGRAPH SYSTEM - MODEL 820MS USER MANUAL...
  • Page 21 4. Measurement Range: The MEASUREMENT RANGE sub-menu in SETTINGS determines the maxi- mum force capacity of the transducer. The factory setting is 200 mN, but the transducer capacity can be changed to 400 mN, 800 mN or a maximum of 1600 mN of force detection, depending on the size of the vessel used. The “SELECT”...

  • Page 22: Interface Menus - Direct Mode

    The signals produced from the transducers are not processed (not channelled through an amplifier). The total voltage output from the BNC connectors is ±2.5 volts and the effective force range will be 600mN. Power-Up Screen After turning on the 820MS Interface, an “Introduction” screen appears. MUSCLE STRIP MYOGRAPH SYSTEM - MODEL 820MS USER MANUAL...
  • Page 23 After a few seconds, the DMT 820MS Direct Force Output display will ap- pear. When in Direct Mode, the ACTUAL FORCE display will not be shown, as is the case when the system is in the Calibrated Mode (see “3.2 Interface Menus –...

  • Page 24: Chapter 4 - The Muscle Strip Myograph Unit

    CHAPTER 4 - THE MUSCLE STRIP MYOGRAPH UNIT Chapter 4 contains a complete explanation of how to calibrate and maintain the 820MS myograph to ensure the equipment is always performing at peak performance. This Chapter also covers the mounting supports and how to mount a muscle strip in the 820MS chamber.
  • Page 25 Muscle with relative large contractile forces (>200mN) For muscle strips of interest producing relatively large contractile forces, the following calibration procedure is recommended. For example, muscle strips that may potentially be producing forces around 300 mN, then the FORCE REC. OUTPUT and MEAS- UREMENT RANGE options should be set appropriately (see “3.2 Interface Menus –...

  • Page 26: Checking The Force Transducer

    AND the transducer cannot be recalibrated, then the force transducer is broken and needs to be replaced. If any other problems related to the force transducer are encountered, please contact DMT for advice or further instructions. 4.3 Force Transducer Replacement If the force transducer breaks and needs to be replaced, follow this step-by-step replacement procedure carefully: 1.
  • Page 27 4. The replacement transducer will be shipped with the new transducer inside a new transducer house. 5. Place a VERY small amount of vacuum grease (clear or whitish grease) around the bottom inside of the transducer housing to seal the transducer housing when put back in place. An arrow in figure 4.5 below indicate the place that the grease needs to be applied.

  • Page 28: Myograph Maintenance

    4.4 Myograph Maintenance The Muscle Strip Myograph System - 820MS is a very delicate and sophisticated piece of research equipment. DMT recommends that the following sections are read carefully and that the instructions are followed at all times. Myograph chamber tubing To prevent the tubing from becoming blocked with buffer salt deposits after an experiment, remove the chamber cover from the Myograph Chamber.

  • Page 29: Cleaning The Myograph

    4.5 Cleaning the myograph DMT strongly recommends that the myograph chambers and surrounding areas are cleaned after each experiment. At the end of each experiment, use the following procedure to clean the myograph chambers and supports: 1. Fill the myograph chamber to the edge with an 8% acetic acid solution and allow it to work for a few minutes to dissolve calcium deposits and other salt build-up.

  • Page 30: Chapter 5 - The Mounting Supports And Mounting In The 820Ms Chamber

    CHAPTER 5 - THE MOUNTING SUPPORTS AND MOUNTING IN THE 820MS CHAM- Each chamber contains supports with mounting clamps to facilitate the mounting of muscle strips in the myograph chambers. These clamps have been designed to optimally “clamp” the tissue without slipping during a contraction. They are also designed to effectively “grab”...

  • Page 31: Appendix 1 - System Specifications

    APPENDIX 1 - SYSTEM SPECIFICATIONS Technical specifications Vessel size: >3 mm - 19 mm Chamber: Four individual chambers Chamber material: Acid-resistant stainless steel Chamber volume: Max. 4 ml Chamber suction: Manual or automatic, time controlled, user defined Chamber cover: Supplied with connections for gassing Individually controlled per chamber by needle valves Chamber gassing: Force range:...

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